GLIS2 (GLIS family zinc finger 2)

2013-04-01   Clarisse Thiollier 

Gustave Roussy Institute, Villejuif, France

Identity

HGNC
LOCATION
16p13.3
LOCUSID
ALIAS
NKL,NPHP7
FUSION GENES

DNA/RNA

Note

Size: 7383 b; 6 coding exons.

Transcription

2 transcripts: 3,7 kb RNA and 4,469 kb RNA (Ensembl).

Proteins

Note

524 amino acids; molecular weight: 55,688 kD.
Member of the Krüppel-like zinc finger proteins family, forming a subfamily with Glis1 and Glis3 closely related to Gli and Zic protein families.

Description

Similarly to Gli and Zic proteins, Glis2 contains 5 Cys2-His2 zinc finger motifs separated by a conserved consensus region T/SGEKPY/FX (Zhang et al., 2002).

Expression

In mouse tissues: high expression level of Glis2 in kidney, moderate level in heart, lung, low level in prostate, brain, colon (Zhang et al., 2002).
In rat embryos: high expression in embryonic kidneys (particularly within the ureteric bud in 16-dpc and 19-dpc rat).
Expression of Glis2 detected in caudal somites and in the neural tubes of 13-dpc in rat embryos (Zhang et al., 2002); high expression level in neural tube in Xenopus, mouse and chick embryos (Lamar et al., 2001).
In human: GLIS2 mRNA is highly expressed in kidney, low level in heart, lung and placenta (Zhang and Jetten, 2001).

Localisation

Nuclear localization (Zhang et al., 2002); the 3rd Zinc Finger motif contains a region required for the nuclear localization (Vasanth et al., 2011).

Function

Krüppel-like zinc finger family proteins can act as activator or repressor of gene transcription. They are involved in regulation of embryonic development and various physiological mechanisms in adults (Dang et al., 2000).
Kang and colleagues described the regulation of gene expression by Glis1-3 through the interaction with transcriptional mediators that are recruited by specific repressor and activation domains within the respective Glis protein.
According to cell context, Glis2 can thus act as a transcriptional activator or repressor. Glis2 is especially implicated in the maintenance of normal kidney structure and function; both activation and repression roles are involved during embryonic development and adult kidney (Zhang et al., 2002; Attanasio et al., 2007).
GLIS2 has also been described as NKL (neuronal Krüppel-like protein): NKL is broadly expressed in the neural tube and peripheral nervous system when neural precursors are differentiating. NKL is implicated in neurogenesis, promoting neuronal differentiation (Lamar et al., 2001).
In mice, Zhang and colleagues (Zhang et al., 2002) highlighted the presence of an activation domain located between Gly71 and Gly137. Two repressor domains were identified: one is located between Ser148 and Arg171 and the other is located within the first zinc finger motif. CtBP1 (C-terminal binding protein 1) and HDAC3 (histone deacetylase 3) are two co-repressor recruited by Glis2 for its repression activity, though a repression complex (Kim et al., 2005).

Homology

GLI proteins are homologous of Cubitus interruptus (Ci) in Drosophila melanogaster which plays an important role in wing development. Ci and GLI are involved in Sonic-Hedgehog pathway in Dosophila and vertebrates. (Zhang and Jetten, 2001).
Glis2 shows high homologies with Gli and Zic Krüppel-like protein families.
In mice highest homology is observed in the zinc finger motifs: 57%, 56%, 56% and 49% homology with Gli1, Gli2, Gli3 and Zic1 respectively. 3rd and 5th zinc finger domains show the highest homology with Gli proteins (70 and 88% homology) (Zhang et al., 2002).
In human: highest homology is observed with GLI1: 58% between Zinc Finger domains. 3rd, 4th and 5th Zinc Finger show the highest homology: 65-80% homology when compared to those of GLI and ZIC proteins (Zhang and Jetten, 2001).
This suggests that Glis2 interacts with DNA in the way Gli and Zic proteins do (Zhang et al., 2002).
In both human and mouse, little homology with Gli and Zic protein family is observed outside the zinc finger domain, which show that Glis2 belongs to another subfamily of Krüppel-like zinc finger proteins (Zhang et al., 2002; Zhang and Jetten, 2001).

Implicated in

Entity name
Acute megakaryoblastic leukemia (AMKL, AML-M7 (FAB classification))
Note
CBFA2T3-GLIS2 or ETO2-GLIS2 fusion oncogene (Thiollier et al., 2012; Gruber et al., 2012; Masetti et al., 2013).
For the first time, GLIS2 has been implicated in the haematological context. GLIS2 is indeed not expressed in normal hematopoietic cells. Its fusion to CBFA2T3 leads to the expression of GLIS2 in blasts.
Prognosis
Worse overall survival at 5 years than other pediatric AMKL patients.
Cytogenetics
The ETO2-GLIS2 fusion oncogene results from a chromosomal inversion inv(16)(p13.3q24.3). This is a cryptic inversion not visible by on a karyotype.
Fusion protein
Different fusions points have been described: 1) between exon 11 of CBFA2T3 and exon 3 of GLIS2 (most frequent); 2) between exon 10 of CBFA2T3 and exon 2 of GLIS2; 3) between exon 12 of CBFA2T3 and exon 1 of GLIS2. All fusion proteins are predicted to contain the GLIS2 DNA binding C-terminal Zinc Finger domains and the three CBFA2T3 N-terminal nervy homology regions that mediate protein interactions.
Entity name
Nephronophthisis (NPHP)
Disease
Nephronophthisis, an autosomal recessive kidney disease.
Cytogenetics
Mutation in GLIS2 gene: homozygous transversion IVS5+1G>T that leads to an abrogation of the obligatory splice site and to a non-functional protein. This mutation defines a new cause of NPHP (Attanasio et al., 2007).

Bibliography

Pubmed IDLast YearTitleAuthors
176182852007Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis.Attanasio M et al
111374512000The biology of the mammalian Krüppel-like family of transcription factors.Dang DT et al
231535402012An Inv(16)(p13.3q24.3)-encoded CBFA2T3-GLIS2 fusion protein defines an aggressive subtype of pediatric acute megakaryoblastic leukemia.Gruber TA et al
163268622005Krüppel-like zinc finger protein Gli-similar 2 (Glis2) represses transcription through interaction with C-terminal binding protein 1 (CtBP1).Kim SC et al
112622342001Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation.Lamar E et al
234075492013CBFA2T3-GLIS2 fusion transcript is a novel common feature in pediatric, cytogenetically normal AML, not restricted to FAB M7 subtype.Masetti R et al
230456052012Characterization of novel genomic alterations and therapeutic approaches using acute megakaryoblastic leukemia xenograft models.Thiollier C et al
211270752011Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2).Vasanth S et al
117388172001Genomic structure of the gene encoding the human GLI-related, Krüppel-like zinc finger protein GLIS2.Zhang F et al
117419912002Characterization of Glis2, a novel gene encoding a Gli-related, Krüppel-like transcription factor with transactivation and repressor functions. Roles in kidney development and neurogenesis.Zhang F et al

Other Information

Locus ID:

NCBI: 84662
MIM: 608539
HGNC: 29450
Ensembl: ENSG00000126603

Variants:

dbSNP: 84662
ClinVar: 84662
TCGA: ENSG00000126603
COSMIC: GLIS2

RNA/Proteins

Gene IDTranscript IDUniprot
ENSG00000126603ENST00000262366Q9BZE0
ENSG00000126603ENST00000433375Q9BZE0

Expression (GTEx)

0
10
20
30
40
50
60
70
80
90

References

Pubmed IDYearTitleCitations
176182852007Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis.104
235594092013Identification of 99 novel mutations in a worldwide cohort of 1,056 patients with a nephronophthisis-related ciliopathy.82
231535402012An Inv(16)(p13.3q24.3)-encoded CBFA2T3-GLIS2 fusion protein defines an aggressive subtype of pediatric acute megakaryoblastic leukemia.69
210681282011Mutation analysis of 18 nephronophthisis associated ciliopathy disease genes using a DNA pooling and next generation sequencing strategy.60
117419912002Characterization of Glis2, a novel gene encoding a Gli-related, Krüppel-like transcription factor with transactivation and repressor functions. Roles in kidney development and neurogenesis.36
173444762007The transcriptional repressor Glis2 is a novel binding partner for p120 catenin.34
172890292007The Krüppel-like zinc finger protein Glis2 functions as a negative modulator of the Wnt/beta-catenin signaling pathway.23
211270752011Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2).14
282924422017ETO2-GLIS2 Hijacks Transcriptional Complexes to Drive Cellular Identity and Self-Renewal in Pediatric Acute Megakaryoblastic Leukemia.12
241275502013DHH-RHEBL1 fusion transcript: a novel recurrent feature in the new landscape of pediatric CBFA2T3-GLIS2-positive acute myeloid leukemia.11

Citation

Clarisse Thiollier

GLIS2 (GLIS family zinc finger 2)

Atlas Genet Cytogenet Oncol Haematol. 2013-04-01

Online version: http://atlasgeneticsoncology.org/gene/44598/glis2