MVP ( major vault protein)

Identity

Other names	LRP (lung resistance-related protein)
Hugo	MVP
Location	16p11.2

Note	drug resistance gene, codes for a "lung resistance-related protein" (LRP) originally detected in a lung carcinoma cell line resistant to chemotherapy

DNA/RNA

Transcription

coding sequence, corresponding to full mRNA, is 2840 bp long

Protein

Description	110 KD protein, 896 amino acids; major cytoplasmic vault protein; vaults are nucleoproteic complexes composed of the MVP associated with two high-molecular weight proteins p240 and p193 which surround a small 140 nucleotides RNA species
Expression	expressed in a variety of human tumor cell lines, the major part being multidrug resistant; expressed also in primary solid tumors, mainly colon carcinoma or ovarian carcinoma, as well as in hematopoietic cancers (myelodysplastic syndrome, acute myeloid or lymphoid leukemia, multiple myeloma); in normal tissues, LRP expression has a wide distribution, with particularly high levels in epithelial cells of the digestive tract
Localisation	cytoplasmic, in the cytosol, with a little fraction (approximately 5%) located in the nuclear membrane and nuclear pores
Function	vault are cytoplasmic organelles which mediate bidirectional nucleocytoplasmic transport of a wide range of substrates, including cytotoxic drugs; vaults would be involved both in vesicule and cytosolic transport of molecules
Homology	104 kD rat MVP

Implicated in

Entity	implicated in induced multidrug-resistance to anticancer chemotherapy
Disease	it includes resistance to doxorubicin, vinca alkaloids, mitoxantrone and etoposide; the role of LRP by itself in multidrug-resistance is not completely clear because it is only a part of a nucleo-proteic complex involved in macromolecules transport; the introduction and expression of the LRP gene in cells by transfection does not confer a multidrug-resistance phenotype; overexpression of LRP has been associated with poor response to chemotherapy in various types of leukemia and in ovarian carcinoma, however the role of LRP as pronostic factor remains controversial; in multi-resistant cell lines, LRP amplification within a homogeneous staining region is exceptional

External links

	Nomenclature
Hugo	MVP
GDB	MVP
Entrez_Gene	MVP  9961  major vault protein
	Cards
Atlas	LRPID120
GeneCards	MVP
Ensembl	MVP [Search_View]   ENSG00000013364 [Gene_View]
Genatlas	MVP
GeneLynx	MVP
eGenome	MVP
euGene	9961
	Genomic and cartography
GoldenPath	MVP  -  16p11.2   chr16:29739289-29766842 +  16p13.1-p11.2   [Description]    (hg18-Mar_2006)
Ensembl	MVP - 16p13.1-p11.2 [CytoView]
NCBI	Mapview
OMIM	Disease map [OMIM]
HomoloGene	MVP
	Gene and transcription
Genbank	AJ291366 [ ENTREZ ]
Genbank	AJ291367 [ ENTREZ ]
Genbank	BC008932 [ ENTREZ ]
Genbank	BC015623 [ ENTREZ ]
Genbank	CR618185 [ ENTREZ ]
RefSeq	NM_005115 [ SRS ]    NM_005115 [ ENTREZ ]
RefSeq	NM_017458 [ SRS ]    NM_017458 [ ENTREZ ]
RefSeq	AC_000059 [ SRS ]    AC_000059 [ ENTREZ ]
RefSeq	NC_000016 [ SRS ]    NC_000016 [ ENTREZ ]
RefSeq	NT_010393 [ SRS ]    NT_010393 [ ENTREZ ]
RefSeq	NW_926539 [ SRS ]    NW_926539 [ ENTREZ ]
AceView	MVP AceView - NCBI
Unigene	Hs.632177 [ SRS ]    Hs.632177 [ NCBI ]     HS632177 [ spliceNest ]
Fast-db	6887 (alternative variants)
	Protein : pattern, domain, 3D structure
SwissProt	Q14764 [ SRS]    Q14764 [ EXPASY ]     Q14764 [ INTERPRO ]
Prosite	PS51224 MVP [ SRS ]    PS51224 MVP [ Expasy ]
Interpro	IPR002499 Vault_N [ SRS ]    IPR002499 Vault_N [ EBI ]
CluSTr	Q14764
Pfam	PF01505 Vault [ SRS ]    PF01505 Vault [ Sanger ]    pfam01505 [ NCBI-CDD ]
Blocks	Q14764
PDB	1Y7X [ SRS ]    1Y7X [ PdbSum ],   1Y7X [ IMB ]   1Y7X [ RSDB ]
HPRD	05475
	Protein Interaction databases
DIP	Q14764
IntAct	Q14764
	Polymorphism : SNP, mutations, diseases
OMIM	605088    [ map ]
GENECLINICS	605088
SNP	MVP [dbSNP-NCBI]
SNP	NM_005115 [SNP-NCI]
SNP	NM_017458 [SNP-NCI]
SNP	MVP [GeneSNPs - Utah]  MVP] [HGBASE - SRS]
HAPMAP	MVP [HAPMAP]
HGMD	MVP
	General knowledge
Family Browser	MVP [UCSC Family Browser]
SOURCE	NM_005115
SOURCE	NM_017458
SMD	Hs.632177
SAGE	Hs.632177
GO	protein binding [Amigo]  protein binding
GO	nucleus [Amigo]  nucleus
GO	nuclear pore [Amigo]  nuclear pore
GO	cytoplasm [Amigo]  cytoplasm
GO	protein transport [Amigo]  protein transport
GO	ribonucleoprotein complex [Amigo]  ribonucleoprotein complex
GO	response to drug [Amigo]  response to drug
GO	mRNA transport [Amigo]  mRNA transport
GO	intracellular protein transport across a membrane [Amigo]  intracellular protein transport across a membrane
PubGene	MVP
TreeFam	MVP
CTD	9961 [Comparative ToxicoGenomics Database]
	Other databases
	Probes
Probe	MVP Related clones (RZPD - Berlin)
	PubMed
PubMed	49 Pubmed reference(s) in LocusLink

Bibliography

Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line.

Cole SP, Bhardwaj G, Gerlach JH, Mackie JE, Grant CE, Almquist KC, Stewart AJ, Kurz EU, Duncan AM, Deeley RG

Science (New York, N.Y.). 1992 ; 258 (5088) : 1650-1654.

PMID 1360704

The LRP gene encoding a major vault protein associated with drug resistance maps proximal to MRP on chromosome 16: evidence that chromosome breakage plays a key role in MRP or LRP gene amplification.

Slovak ML, Ho JP, Cole SP, Deeley RG, Greenberger L, de Vries EG, Broxterman HJ, Scheffer GL, Scheper RJ

Cancer research. 1995 ; 55 (19) : 4214-4219.

PMID 7671223

Major vault protein LRP-related multidrug resistance.

Izquierdo MA, Scheffer GL, Flens MJ, Schroeijers AB, van der Valk P, Scheper RJ

European journal of cancer (Oxford, England : 1990). 1996 ; 32A (6) : 979-984.

PMID 8763338

Expression of the human major vault protein LRP in acute myeloid leukemia.

Hart SM, Ganeshaguru K, Scheper RJ, Prentice HG, Hoffbrand AV, Mehta AB

Experimental hematology. 1997 ; 25 (12) : 1227-1232.

PMID 9357965

Increased LRP mRNA expression is associated with the MDR phenotype in intrinsically resistant human cancer cell lines.

Laurenˆßot CM, Scheffer GL, Scheper RJ, Shoemaker RH

International journal of cancer. Journal international du cancer. 1997 ; 72 (6) : 1021-1026.

PMID 9378536

Coexpression of multidrug resistance involve proteins: a flow cytometric analysis.

Boutonnat J, Bonnefoix T, Mousseau M, Seigneurin D, Ronot X

Anticancer research. 1998 ; 18 (4C) : 2993-2999.

PMID 9713498

P-glycoprotein (PGP), and not lung resistance-related protein (LRP), is a negative prognostic factor in secondary leukemias.

Damiani D, Michieli M, Ermacora A, Candoni A, Raspadori D, Geromin A, Stocchi R, Grimaz S, Masolini P, Michelutti A, Scheper RJ, Baccarani M

Haematologica. 1998 ; 83 (4) : 290-297.

PMID 9592977

Relationship between major vault protein/lung resistance protein, multidrug resistance-associated protein, P-glycoprotein expression, and drug resistance in childhood leukemia.

den Boer ML, Pieters R, Kazemier KM, Rottier MM, Zwaan CM, Kaspers GJ, Janka-Schaub G, Henze G, Creutzig U, Scheper RJ, Veerman AJ

Blood. 1998 ; 91 (6) : 2092-2098.

PMID 9490695

Expression of the lung resistance protein predicts poor outcome in de novo acute myeloid leukemia.

Filipits M, Pohl G, Stranzl T, Suchomel RW, Scheper RJ, Jˆ§ger U, Geissler K, Lechner K, Pirker R

Blood. 1998 ; 91 (5) : 1508-1513.

PMID 9473213

Multidrug resistance in leukemia.

Leith C

Current opinion in hematology. 1998 ; 5 (4) : 287-291.

PMID 9747635

Expression of lung resistance protein and correlation with other drug resistance proteins and outcome in myelodysplastic syndromes.

Lepelley P, Poulain S, Grardel N, Preudhomme C, Cosson A, Fenaux P

Leukemia & lymphoma. 1998 ; 29 (5-6) : 547-551.

PMID 9643568

Lung-resistance-related protein expression is a negative predictive factor for response to conventional low but not to intensified dose alkylating chemotherapy in multiple myeloma.

Raaijmakers HG, Izquierdo MA, Lokhorst HM, de Leeuw C, Belien JA, Bloem AC, Dekker AW, Scheper RJ, Sonneveld P

Blood. 1998 ; 91 (3) : 1029-1036.

PMID 9446666

Multidrug resistance and its reversal.

Volm M

Anticancer research. 1998 ; 18 (4C) : 2905-2917.

PMID 9713485

Expression of the lung resistance protein predicts poor outcome in patients with multiple myeloma.

Filipits M, Drach J, Pohl G, Schuster J, Stranzl T, Ackermann J, Kˆ�nigsberg R, Kaufmann H, Gisslinger H, Huber H, Ludwig H, Pirker R

Clinical cancer research : an official journal of the American Association for Cancer Research. 1999 ; 5 (9) : 2426-2430.

PMID 10499614

Increased expression of multidrug resistance related proteins Pgp, MRP1, and LRP/MVP occurs early in colorectal carcinogenesis.

Meijer GA, Schroeijers AB, Flens MJ, Meuwissen SG, van der Valk P, Baak JP, Scheper RJ

Journal of clinical pathology. 1999 ; 52 (6) : 450-454.

PMID 10562814

Expression of the lung resistance protein (LRP) in primary breast cancer.

Pohl G, Filipits M, Suchomel RW, Stranzl T, Depisch D, Pirker R

Anticancer research. 1999 ; 19 (6B) : 5051-5055.

PMID 10697509

P-glycoprotein and multidrug resistance-associated protein, but not lung resistance protein, lower the intracellular daunorubicin accumulation in acute myeloid leukaemic cells.

Borg AG, Burgess R, Green LM, Scheper RJ, Liu Yin JA

British journal of haematology. 2000 ; 108 (1) : 48-54.

PMID 10651723

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Contributor(s)

Written	05-2000	Franck Viguié

Citation

This paper should be referenced as such :

Viguié F . MVP ( major vault protein). Atlas Genet Cytogenet Oncol Haematol. May 2000 . URL : http://AtlasGeneticsOncology.org/Genes/LRPID120.html

© Atlas of Genetics and Cytogenetics in Oncology and Haematology

indexed on : Wed Jul 2 08:24:43 2008