NONO

Identity

Hugo	NONO
Location	Xq12

DNA/RNA

Transcription

alternative splicing; 2.4 mRNA complete cds; coding sequence: CDS 129 ... 1550

Protein

Description	the protein encoded by NONO is called p54nrb (nuclear RNA binbing); 471 amino acids; 54 kDa; N-term glutamine/histidine rich region, tandem RNA binding domains (amino acids 75 to 228), helix-turn-helix implicated in DNA binding, and a proline rich region in C-term; N-term and C-term allow protein-protein interactions.
Expression	wide
Function	binds independantly to DNA and RNA; forms a heterodimer with PSF, a protein sharing vast homologies; both form complexes with DNA topoisomerase I, which renders this enzyme much more active; NONO also enhances the binding of several DNA-binding proteins (but not all).
Homology	with PSF and other proteins with a DBHS domain (Drosophila behaviour, human splicing) which includes the tandem RNA binding domains.

Implicated in

Entity	inv(X)(p11.2q12) in renal cell carcinoma -->NonO/TFE3
Disease	only one case of papillary renal cell carcinoma
Hybrid/Mutated Gene	5' NONO- 3' TFE3
Abnormal Protein	N-term NONO and most of it except the C-term proline rich sequence fused to the DNA binding domains of TFE3 (excluding the acidic transcriptional activation domain, including the C-term helix-loop-helix, and the leucine zipper); the reciprocal transcript is found

External links

	Nomenclature
Hugo	NONO
GDB	NONO
Entrez_Gene	NONO  4841  non-POU domain containing, octamer-binding
	Cards
Atlas	NONOID168
GeneCards	NONO
Ensembl	NONO [Search_View]   ENSG00000147140 [Gene_View]
Genatlas	NONO
GeneLynx	NONO
eGenome	NONO
euGene	4841
	Genomic and cartography
GoldenPath	NONO  -  Xq12   chrX:70420192-70437741 +  Xq13.1    (hg18-Mar_2006)
Ensembl	NONO - Xq13.1 [CytoView]
NCBI	Mapview
OMIM	Disease map [OMIM]
HomoloGene	NONO
	Gene and transcription
Genbank	AK055406 [ ENTREZ ]
Genbank	AK291140 [ ENTREZ ]
Genbank	AU119361 [ ENTREZ ]
Genbank	BC002364 [ ENTREZ ]
Genbank	BC003129 [ ENTREZ ]
RefSeq	NM_007363 [ SRS ]    NM_007363 [ ENTREZ ]
RefSeq	AC_000066 [ SRS ]    AC_000066 [ ENTREZ ]
RefSeq	NC_000023 [ SRS ]    NC_000023 [ ENTREZ ]
RefSeq	NT_011669 [ SRS ]    NT_011669 [ ENTREZ ]
RefSeq	NW_927711 [ SRS ]    NW_927711 [ ENTREZ ]
AceView	NONO AceView - NCBI
Unigene	Hs.700344 [ SRS ]    Hs.700344 [ NCBI ]     HS700344 [ spliceNest ]
Fast-db	10541 (alternative variants)
	Protein : pattern, domain, 3D structure
SwissProt	Q15233 [ SRS]    Q15233 [ EXPASY ]     Q15233 [ INTERPRO ]
Prosite	PS50102 RRM [ SRS ]    PS50102 RRM [ Expasy ]
Interpro	IPR012677 a_b_plait_nuc_bd [ SRS ]    IPR012677 a_b_plait_nuc_bd [ EBI ]
Interpro	IPR012975 NOPS [ SRS ]    IPR012975 NOPS [ EBI ]
Interpro	IPR000504 RRM_RNP1 [ SRS ]    IPR000504 RRM_RNP1 [ EBI ]
CluSTr	Q15233
Pfam	PF08075 NOPS [ SRS ]    PF08075 NOPS [ Sanger ]    pfam08075 [ NCBI-CDD ]
Pfam	PF00076 RRM_1 [ SRS ]    PF00076 RRM_1 [ Sanger ]    pfam00076 [ NCBI-CDD ]
Smart	SM00360 RRM [EMBL]
Blocks	Q15233
HPRD	02098
	Protein Interaction databases
DIP	Q15233
IntAct	Q15233
	Polymorphism : SNP, mutations, diseases
OMIM	300084    [ map ]
GENECLINICS	300084
SNP	NONO [dbSNP-NCBI]
SNP	NM_007363 [SNP-NCI]
SNP	NONO [GeneSNPs - Utah]  NONO] [HGBASE - SRS]
HAPMAP	NONO [HAPMAP]
COSMIC	NONO [Somatic mutation (COSMIC-CGP-Sanger)]
HGMD	NONO
	General knowledge
Family Browser	NONO [UCSC Family Browser]
SOURCE	NM_007363
SMD	Hs.700344
SAGE	Hs.700344
GO	nucleotide binding [Amigo]  nucleotide binding
GO	DNA binding [Amigo]  DNA binding
GO	RNA binding [Amigo]  RNA binding
GO	nucleus [Amigo]  nucleus
GO	DNA repair [Amigo]  DNA repair
GO	DNA recombination [Amigo]  DNA recombination
GO	transcription [Amigo]  transcription
GO	regulation of transcription, DNA-dependent [Amigo]  regulation of transcription, DNA-dependent
GO	mRNA processing [Amigo]  mRNA processing
GO	RNA splicing [Amigo]  RNA splicing
GO	identical protein binding [Amigo]  identical protein binding
BIOCARTA	RNA polymerase III transcription    [Genes]
PubGene	NONO
TreeFam	NONO
	Other databases
	Probes
Probe	NONO Related clones (RZPD - Berlin)
	PubMed
PubMed	36 Pubmed reference(s) in LocusLink

Bibliography

Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6.

Dong B, Horowitz DS, Kobayashi R, Krainer AR

Nucleic acids research. 1993 ; 21 (17) : 4085-4092.

PMID 8371983

NonO, a non-POU-domain-containing, octamer-binding protein, is the mammalian homolog of Drosophila nonAdiss.

Yang YS, Hanke JH, Carayannopoulos L, Craft CM, Capra JD, Tucker PW

Molecular and cellular biology. 1993 ; 13 (9) : 5593-5603.

PMID 8355702

Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma.

Clark J, Lu YJ, Sidhar SK, Parker C, Gill S, Smedley D, Hamoudi R, Linehan WM, Shipley J, Cooper CS

Oncogene. 1997 ; 15 (18) : 2233-2239.

PMID 9393982

NonO enhances the association of many DNA-binding proteins to their targets.

Yang YS, Yang MC, Tucker PW, Capra JD

Nucleic acids research. 1997 ; 25 (12) : 2284-2292.

PMID 9171077

The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction.

Straub T, Grue P, Uhse A, Lisby M, Knudsen BR, Tange TO, Westergaard O, Boege F

The Journal of biological chemistry. 1998 ; 273 (41) : 26261-26264.

PMID 9756848

REVIEW articles	automatic search in PubMed
Last year publications	automatic search in PubMed

Search in all EBI   NCBI

Contributor(s)

Written	01-1999	Jean-Loup Huret

Citation

This paper should be referenced as such :

Huret JL . NONO. Atlas Genet Cytogenet Oncol Haematol. January 1999 . URL : http://AtlasGeneticsOncology.org/Genes/NONOID168.html

© Atlas of Genetics and Cytogenetics in Oncology and Haematology

indexed on : Mon May 12 18:06:54 2008