1 CNRS-Pierre Fabre, USR3388, Epigenetic Targeting of Cancer (ETaC), Toulouse, France / MNHN CNRS UMR7196, INSERM U565, Paris, France / Université Pierre et Marie Curie, Paris, France
2 MNHN CNRS UMR7196, INSERM U565, Paris, France
3 CNRS-Pierre Fabre, USR3388, Epigenetic Targeting of Cancer (ETaC), Toulouse, France
Almost every cell in a human organism share the exact same DNA sequence, still it exist more than 200 different cell types that have very distinct functions (e.g fibroblast, neuron, pancreatic cell...). Epigenetic mechanisms participate to instruct cells to acquire and maintain a specific identity. An operational definition of epigenetics is "an epigenetic trait is a stably heritable phenotype resulting from changes in a chromosome without alterations in the DNA sequence" (Berger et al., 2009). In other words epigenetic modifications (marks) modulate gene expression mainly by changing the access to the genetic information. A major modification is DNA methylation, the first epigenetic mark to be identified, and the most studied (Baylin and Jones, 2011). DNA methylation is heritable, does not alter the DNA sequence and is the most stable mark. Its role is critical in normal development and defects in DNA methylation pattern is systematically observed in cancer cells.
I- What is DNA methylation?
DNA methylation is a chemical modification (a methyl group) of the DNA that does not alter the information coded by the DNA but it participates to the modulation of gene expression. In mammals DNA methylation occurs on position 5 of cytosine (C) mainly upstream of guanine (G) in the DNA double-helix, the so-called CpG dinucleotide. This reaction is catalysed by the DNA methyltransferase enzymes (DNMT) that uses the S-adenosyl-L-methionine (AdoMet) as methyl donor leading to 5-methylcytosine (5-mC) (Figure 1).
Figure 1. The DNA methyltransferase (DNMT) catalyses the methylation reaction.
DNMTs are a well-conserved protein family including DNMT1 and DNMT3 that can be distinguish by their main function. DNMT1 (Bestor et al., 1988), which as a high preference for hemi-methylated DNA, is in charge of the "maintenance" of DNA methylation pattern through cell divisions by copying the DNA methylation pattern on the newly synthesized strand. Accordingly, DNMT1 is the most expressed in somatic cells, particularly at the S phase (Robertson et al., 2000; Hermann et al., 2004) (Figure 2). DNMT3A and DNMT3B are involved in de novo DNA methylation that occurs essentially during embryonic development, right after implantation and also during the male and female gametogenesis (Kafri et al., 1992; Okano et al., 1999). DNMT3L is a non-catalytic co-factor for DNMT3A and B that enhance their activity.
Beside DNA methylation, other DNA modification have been reported, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), and are currently widely studied in particular for their role in active DNA demethylation (Franchini et al., 2012; Fu and He, 2012).
Figure 2. Role of the DNMTs. DNMT1 is mainly involved in DNA methylation maintenance while DNMT3A and DNMT3B are de novo methyltransferases. The methyl group on the CpG site is indicated by a red lollipop.
II- Where is DNA methylation located?
In the human genome, CpGs dinucleotides are not randomly distributed but are regrouped in CpG islands. Actually, CpGs are under-represented in the genome (1% versus 4% expected) due to the chemical instability of the 5-mC that is spontaneously, but sporadically, converted into thymine (T) by deamination inducing a C to T transition (Schorderet and Gartler, 1992). However, some parts of the genome are enriched in CpGs and show dense DNA methylation:
- Repeated sequences (LINE and SINE retrotransposons, satellite DNA at centromeres) that represent near half of the genome are the major site of DNA methylation (Yoder et al., 1997).
- In human, around 65% of genes contain in their promoter (including first exons) CpG islands. These islands have been defined by regions of at least 200 base pairs, containing more than 55% of GC and a ratio between the observed CpGs observed and the expected CpGs higher than 0.65 (Gardiner-Garden and Frommer, 1987; Bestor et al., 1988; Takai and Jones, 2002). Importantly, most of these CpG islands are not methylated in somatic cells. Those that harbour DNA methylation are related to tissue-specific and imprinting genes and have an important role in defining the identity of the cell.
III- What is the role of DNA methylation?
DNA methylation is essential for normal embryonic development. Mice Knock-Out for DNMT1, 3A and 3B lead to early embryos death (8.5 to 10.5 dpc) or a few weeks after birth (Li et al., 1992; Okano et al., 1999). In human, the ICF syndrome (Immunodeficiency, Centromere instability, Facial anomalies) is a rare autosomal recessive disease caused by mutations in DNMT3B gene. It is characterized by loss of centromeric methylation and genome instability (Hansen et al., 1999). Indeed DNA methylation has a critical role in genome integrity by repressing transcription at repeated sequences, including highly mutagenic retrotransposon elements.
When located in promoters, CpG island DNA hypermethylation is also associated with a repressed chromatin and thus transcription inhibition (Iguchi-Ariga and Schaffner, 1989; Watt and Molloy, 1988; Prendergast and Ziff, 1991; Clozel et al., 2013). Genes located downstream are silenced although their genomic sequence is intact. In differentiated cells, promote DNA methylation is a common way to shut down the expression of genes that are not implicated in the specific function of a liver cell, retina cell, neuron... DNA methylation is also involved in the establishment and maintenance of genomic imprinting and, in female cells, X-chromosome inactivation. In all these cases, DNA methylation is a transcriptional repressor.
In other contexts like in gene bodies, DNA methylation can play an opposite role and rather be associated with an active transcription (Ball et al., 2009). Finally it can be also involved in splicing and in the silencing of alternative promoters.
IV- DNA methylation & cancer
It has been now established that aberrant DNA methylation plays a crucial role, together with genetic alterations, in tumorigenesis and tumor maintenance.
1. Aberrant DNA methylation pattern in cancer cells
In tumors, a global DNA hypomethylation is associated with a local DNA hypermethylation of specific loci (Figure 3). This seems very contradictory but theses two phenomena act at different sites of DNA methylation and participate both to cancer formation.
Figure 3. Aberrant DNA methylation profile in cancer cells. In tumor cells, a global loss of DNA methylation at repeated sequences leading to global hypomethylation of the genome is observed together with DNA hypermethylation at specific sites, such as tumor suppressor genes promoters, inducing the silencing the corresponding gene. White lollipop: non-methylated CpG, red lollipop: methylated CpG.
2. Global DNA hypomethylation of the genome
One of the first epigenetic alterations found in cancer cells was a global decrease in DNA methylation. Repeated sequences, which are highly methylated, are hypomethylated, triggering transcriptional re-activation of parasitic DNA sequences that can randomly integrated into the genome contributing to the high genetic instability of cancer cells (Dante et al., 1992; Alves et al., 1996; Howard et al., 2008). Moreover examples of loss of DNA methylation have been reported at gene promoters leading to their re-expression. This is the case of MAGE gene family that are normally repressed in all cell types at the exception of testicular cells (De Smet et al., 1999). Oncogenes such as R-RAS, RHOB and ELK1 have also been described as re-expressed in gastric cancers following demethylation of their promoters (Nishigaki et al., 2005). All these events participate to tumorigenesis at early steps of the disease.
3. Localized DNA hypermethylation
In the human genome, CpG islands are mainly located in gene promoters and are the targets of DNA hypermethylation in cancers. It is estimated that 5 to 10% of promoters are hypermethylated in cancer leading to the silencing of downstream genes (Bird, 2002). Mainly this process concerns tumor suppressor genes (TSG) thus favoring cancer development. The first TSG that was described to be silenced by hypermethylation was Retinoblastoma (Rb) (Greger et al., 1989), involved in the control of the cell cycle. Next a battery of hypermethylated genes were found to be involved in crucial processes such as DNA repair (BRCA1) (Esteller et al., 2000), cell proliferation (CDKN2A) (Merlo et al., 1995), cell adhesion (CDH1) (Graff et al., 1995), angiogenesis (VHL) (Herman et al., 1994) and other essential functions. Interestingly, each cancer has a DNA methylation profile that can be used as signature to develop diagnostic biomarkers (Costello et al., 2000; Esteller et al., 2001; Paz et al., 2003). The first commercial DNA methylation tests for diagnosis are based on the detection of Septin9 hypermethylation in blood plasma for colon cancer (ColoVantage® test) and GSTPI hypermethylation in urine for prostate cancer (test by LabCorp). In addition, promoter DNA hypermethylation can be used to predict the response to treatment, such as MGMT hypermethylation in glioblastoma patients for temozolomide treatment (test by MDxHealth).
Since loss of TSG expression by aberrant DNA methylation gives to tumor cells a strong proliferative advantage, DNA hypermethylation could be set up randomly and only DNA methylation profiles conferring a selective advantage would be maintained in the tumor (as for genetic mutations). However several observations pointed out that cancer DNA hypermethylation can be an instructive mechanism. DNA methylation is an early event of the tumor formation (Issa, 2004; Feinberg et al., 2006; Troyer et al., 2009). Short DNA sequences are found enriched at hypermethylated promoters suggesting a sequence-specific targeting (Feltus et al., 2003; Feltus et al., 2006; Keshet et al., 2006). Interestingly, entire chromosome regions can be subject to the same epigenetic repression (LRES: Long-Range Epigenetic Silencing) (Frigola et al., 2006; Coolen et al., 2010) or reactivation (LREA: Long-Range Epigenetic Activation) (Bert et al., 2013). Hypermethylation can cluster on chromosomes, which are delimitated by insulator proteins like CTCF (Witcher and Emerson, 2009); LINE and SINE retrotransposons sequences protect adjacent promoters from DNA methylation (Estécio et al., 2010); polycomb target genes are predispose to hypermethylation (Ohm et al., 2007; Schlesinger et al., 2007; Widschwendter et al., 2007); and non-coding RNAs can also direct specifically DNA hypermethylation by interacting with DNMT (Di Ruscio et al., 2013). It seems that many parameters (gene localisation, promoter sequence, transcription activity, developmental program...) are, all together, important to define if a promoter will be subjected to DNA methylation during tumorigenesis.
V- Targeting DNA methylation in cancers
The study of the cancer epigenome - the ensemble of epigenetic marks on the genome - is of great interest as it can help clinical diagnosis, prognostic and treatment strategies. Today several consortium are built to determine the human epigenomes in several contexts, such as the Human Epigenome Project (HEP, at http://www.epigenome.org/) that aims at measuring the DNA methylation profile in all major tissues or such as The Cancer Genome Atlas (TCGA at
https://tcga-data.nci.nih.gov/tcga/tcgaHome2.jsp) that determine the genetic and epigenetic profiles in cancers.
Noteworthy, by altering DNA methylation cells modulate at once several biological processes and signaling cascades, making its targeting a therapeutic advantage (Azad et al., 2013).
VI- Present and the future of DNA methylation cancer therapy
In addition, contrary to genetic mutations, epimutations are reversible and thus offer interesting therapeutic perspectives. By targeting DNA methylation, cells are reprogrammed; reexpression of TSG can induce cell arrest, differentiation and death, but also sensitivity to apoptosis, immuno-response and drug treatment. An example is the reversal of the resistance to doxorubicine in diffuse large B-cell lymphoma (DLBL) by low-doses of DNMT inhibitors (Clozel et al., 2013).
It exists two classes of DNMT inhibitors (DNMTi): the nucleoside analogues that are incorporated into nucleic acids and form a covalent complex with the enzyme, and the non-nucleoside inhibitors that present different mechanisms of inhibition.
1. DNMTi: Nucleoside analogues and anti-cancer therapy
Nucleoside analogues are based on ribose or deoxyribose analogs of cytidine, in particular 5-aza-cytidine (5aza) and 5-aza-2'deoxycytidine (5azadC) are used in clinics for the treatment of myeolodysplastic syndromes (MDS) and acute myeloid leukemia (AML) (FDA approval in 2004 and 2006, respectively, for MDS, AML and CMML (chronic myelomonocytic leukemia) (Figure 4).
Figure 4. A) Chemical structure of 5-aza-cytidine, 5-aza-2' deoxycytidine, prodrug SGI110. B) Catalytic reaction by DNMTs. C) Inhibition by 5azacytosine in DNA.
In cells, these compounds are metabolized by kinases to convert them into nucleotides that are incorporated into RNA (5aza) or into DNA (5azadC and 5aza after conversion of the ribose in deoxyribose). Since these compounds need to be integrated into the DNA to be active, they are particularly efficient in highly proliferative cells like cancer ones. During the catalytic reaction, DNMTs binds covalently to position 6 of the cytosine ring and after transfer of the methyl group a β-elimination reaction frees the enzyme (Figure 4B). In the case of the aza-nucleoside the β-elimination cannot occur and the enzyme is trapped on the DNA forming a suicide complex (Figure 4C), and eventually it is degraded by the proteasome. Because of their mechanism of action, these compounds are not selective towards the different DNMTs and thereby induce a global decrease of DNA methylation. In addition, 5aza (Vidaza) and 5azadC (Dacongen) are limited to hemotological cancer because of their chemical instability. Recently, a prodrug, a 5azadCpdG analog, SGI110 (developed by Astex) (Chuang et al., 2010), is in clinical trials for haematological diseases, and solid cancers in combination; the results are encouraging. Indeed, very promising results have been obtained with the 5aza-nucleosides in combination with HDAC inhibitors (Juergens et al., 2011) and/or when used to restore chemosensitivity to anticancer treatments (Clozel et al., 2013; Vijayaraghavalu et al., 2013; Wrangle et al., 2013). Still, these molecules are non-specific toward the DNMTs, act on the whole genome, are chemically instable and have secondary effects in patients (renal toxicity, myelotoxicity), therefore there are continuous efforts aiming at finding non-nucleoside inhibitors.
2. Non-nucleoside inhibitors for DNMT selectivity
Non-nucleoside inhibitors share the common feature of not being incorporated in DNA. However they inhibit the catalytic reaction by different mechanisms of action: they can compete with the cofactor, the AdoMet, the DNA substrate or bind to allosteric sites. Today many are described, of different nature, but few are well characterized for their mechanism of action or their selectivity towards a specific DNMT (for review Fahy et al., 2012; Gros et al., 2012). For example, SGI-1027 belongs to a family of minor groove DNA binders developed by Denny and collaborators (Datta et al., 2009) and has been shown to inhibit DNA methylation in enzymatic tests and in cancer cells. We developed two families of DNMT inhibitors: procainamide conjugates that are selective of DNA methyltransferases versus histone methyltransferases (Halby et al., 2012) and flavanoid derivatives that also inhibit DNA methylation in an in vivo model of zebrafish development (Ceccaldi et al., 2011). Since the small molecules identified so far showed less anti-cancer activities than nucleoside analogs and present limited therapeutic interest, we and others have developed high-throughput screening and drug design strategies to discover new inhibitors (Ceccaldi et al., 2013; Kilgore et al., 2013; Weng et al., 2014).
3. Perspectives in DNMTi
The success of 5aza and 5azad as anti-cancer treatments alone and more recently in combination leads researchers to focus on new compounds with improved bioavaibility and specificity. Many efforts are also dedicated to better understand the mechanism of action in cancer cells of the azanucleoside and their long-term consequences. There is still an urgent need to find new small molecules specific to each DNMTs to better understand the role of each enzyme in tumorigenesis and cancer maintenance and to decrease the off-targets. These new drugs will benefit to cancer patients but not only since abnormal DNA methylation pattern is also involved in other pathologies such as autoimmune and neurological disorders.
|CpG islands in vertebrate genomes.|
|Gardiner-Garden M, Frommer M.|
|J Mol Biol. 1987 Jul 20;196(2):261-82.|
|Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases.|
|Bestor T, Laudano A, Mattaliano R, Ingram V.|
|J Mol Biol. 1988 Oct 20;203(4):971-83.|
|Cytosine methylation prevents binding to DNA of a HeLa cell transcription factor required for optimal expression of the adenovirus major late promoter.|
|Watt F, Molloy PL.|
|Genes Dev. 1988 Sep;2(9):1136-43.|
|Epigenetic changes may contribute to the formation and spontaneous regression of retinoblastoma.|
|Greger V, Passarge E, Hopping W, Messmer E, Horsthemke B.|
|Hum Genet. 1989 Sep;83(2):155-8.|
|CpG methylation of the cAMP-responsive enhancer/promoter sequence TGACGTCA abolishes specific factor binding as well as transcriptional activation.|
|Iguchi-Ariga SM, Schaffner W.|
|Genes Dev. 1989 May;3(5):612-9.|
|Methylation-sensitive sequence-specific DNA binding by the c-Myc basic region.|
|Prendergast GC, Ziff EB.|
|Science. 1991 Jan 11;251(4990):186-9.|
|Methylation patterns of long interspersed repeated DNA and alphoid repetitive DNA from human cell lines and tumors.|
|Dante R, Dante-Paire J, Rigal D, Roizes G.|
|Anticancer Res. 1992 Mar-Apr;12(2):559-63.|
|Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line.|
|Kafri T, Ariel M, Brandeis M, Shemer R, Urven L, McCarrey J, Cedar H, Razin A.|
|Genes Dev. 1992 May;6(5):705-14.|
|Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.|
|Li E, Bestor TH, Jaenisch R.|
|Cell. 1992 Jun 12;69(6):915-26.|
|Analysis of CpG suppression in methylated and nonmethylated species.|
|Schorderet DF, Gartler SM.|
|Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):957-61.|
|Silencing of the VHL tumor-suppressor gene by DNA methylation in renal carcinoma.|
|Herman JG, Latif F, Weng Y, Lerman MI, Zbar B, Liu S, Samid D, Duan DS, Gnarra JR, Linehan WM, et al.|
|Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9700-4.|
|E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas.|
|Graff JR, Herman JG, Lapidus RG, Chopra H, Xu R, Jarrard DF, Isaacs WB, Pitha PM, Davidson NE, Baylin SB.|
|Cancer Res. 1995 Nov 15;55(22):5195-9.|
|5' CpG island methylation is associated with transcriptional silencing of the tumour suppressor p16/CDKN2/MTS1 in human cancers.|
|Merlo A, Herman JG, Mao L, Lee DJ, Gabrielson E, Burger PC, Baylin SB, Sidransky D.|
|Nat Med. 1995 Jul;1(7):686-92.|
|Differential methylation of human LINE-1 retrotransposons in malignant cells.|
|Alves G, Tatro A, Fanning T.|
|Gene. 1996 Oct 17;176(1-2):39-44.|
|Cytosine methylation and the ecology of intragenomic parasites.|
|Yoder JA, Walsh CP, Bestor TH.|
|Trends Genet. 1997 Aug;13(8):335-40. (REVIEW)|
|DNA methylation is the primary silencing mechanism for a set of germ line- and tumor-specific genes with a CpG-rich promoter.|
|De Smet C, Lurquin C, Lethe B, Martelange V, Boon T.|
|Mol Cell Biol. 1999 Nov;19(11):7327-35.|
|The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome.|
|Hansen RS, Wijmenga C, Luo P, Stanek AM, Canfield TK, Weemaes CM, Gartler SM.|
|Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14412-7.|
|DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development.|
|Okano M, Bell DW, Haber DA, Li E.|
|Cell. 1999 Oct 29;99(3):247-57.|
|Aberrant CpG-island methylation has non-random and tumour-type-specific patterns.|
|Costello JF, Fruhwald MC, Smiraglia DJ, Rush LJ, Robertson GP, Gao X, Wright FA, Feramisco JD, Peltomaki P, Lang JC, Schuller DE, Yu L, Bloomfield CD, Caligiuri MA, Yates A, Nishikawa R, Su Huang H, Petrelli NJ, Zhang X, O'Dorisio MS, Held WA, Cavenee WK, Plass C.|
|Nat Genet. 2000 Feb;24(2):132-8.|
|Promoter hypermethylation and BRCA1 inactivation in sporadic breast and ovarian tumors.|
|Esteller M, Silva JM, Dominguez G, Bonilla F, Matias-Guiu X, Lerma E, Bussaglia E, Prat J, Harkes IC, Repasky EA, Gabrielson E, Schutte M, Baylin SB, Herman JG.|
|J Natl Cancer Inst. 2000 Apr 5;92(7):564-9.|
|Differential mRNA expression of the human DNA methyltransferases (DNMTs) 1, 3a and 3b during the G(0)/G(1) to S phase transition in normal and tumor cells.|
|Robertson KD, Keyomarsi K, Gonzales FA, Velicescu M, Jones PA.|
|Nucleic Acids Res. 2000 May 15;28(10):2108-13.|
|A gene hypermethylation profile of human cancer.|
|Esteller M, Corn PG, Baylin SB, Herman JG.|
|Cancer Res. 2001 Apr 15;61(8):3225-9.|
|DNA methylation patterns and epigenetic memory.|
|Genes Dev. 2002 Jan 1;16(1):6-21. (REVIEW)|
|Comprehensive analysis of CpG islands in human chromosomes 21 and 22.|
|Takai D, Jones PA.|
|Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3740-5. Epub 2002 Mar 12.|
|Predicting aberrant CpG island methylation.|
|Feltus FA, Lee EK, Costello JF, Plass C, Vertino PM.|
|Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12253-8. Epub 2003 Sep 30.|
|A systematic profile of DNA methylation in human cancer cell lines.|
|Paz MF, Fraga MF, Avila S, Guo M, Pollan M, Herman JG, Esteller M.|
|Cancer Res. 2003 Mar 1;63(5):1114-21.|
|The Dnmt1 DNA-(cytosine-C5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites.|
|Hermann A, Goyal R, Jeltsch A.|
|J Biol Chem. 2004 Nov 12;279(46):48350-9. Epub 2004 Aug 31.|
|CpG island methylator phenotype in cancer.|
|Nat Rev Cancer. 2004 Dec;4(12):988-93. (REVIEW)|
|Discovery of aberrant expression of R-RAS by cancer-linked DNA hypomethylation in gastric cancer using microarrays.|
|Nishigaki M, Aoyagi K, Danjoh I, Fukaya M, Yanagihara K, Sakamoto H, Yoshida T, Sasaki H.|
|Cancer Res. 2005 Mar 15;65(6):2115-24.|
|The epigenetic progenitor origin of human cancer.|
|Feinberg AP, Ohlsson R, Henikoff S.|
|Nat Rev Genet. 2006 Jan;7(1):21-33. (REVIEW)|
|DNA motifs associated with aberrant CpG island methylation.|
|Feltus FA, Lee EK, Costello JF, Plass C, Vertino PM.|
|Genomics. 2006 May;87(5):572-9. Epub 2006 Feb 17.|
|Epigenetic remodeling in colorectal cancer results in coordinate gene suppression across an entire chromosome band.|
|Frigola J, Song J, Stirzaker C, Hinshelwood RA, Peinado MA, Clark SJ.|
|Nat Genet. 2006 May;38(5):540-9. Epub 2006 Apr 23.|
|Evidence for an instructive mechanism of de novo methylation in cancer cells.|
|Keshet I, Schlesinger Y, Farkash S, Rand E, Hecht M, Segal E, Pikarski E, Young RA, Niveleau A, Cedar H, Simon I.|
|Nat Genet. 2006 Feb;38(2):149-53.|
|A stem cell-like chromatin pattern may predispose tumor suppressor genes to DNA hypermethylation and heritable silencing.|
|Ohm JE, McGarvey KM, Yu X, Cheng L, Schuebel KE, Cope L, Mohammad HP, Chen W, Daniel VC, Yu W, Berman DM, Jenuwein T, Pruitt K, Sharkis SJ, Watkins DN, Herman JG, Baylin SB.|
|Nat Genet. 2007 Feb;39(2):237-42. Epub 2007 Jan 9.|
|Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer.|
|Schlesinger Y, Straussman R, Keshet I, Farkash S, Hecht M, Zimmerman J, Eden E, Yakhini Z, Ben-Shushan E, Reubinoff BE, Bergman Y, Simon I, Cedar H.|
|Nat Genet. 2007 Feb;39(2):232-6. Epub 2006 Dec 31.|
|Epigenetic stem cell signature in cancer.|
|Widschwendter M, Fiegl H, Egle D, Mueller-Holzner E, Spizzo G, Marth C, Weisenberger DJ, Campan M, Young J, Jacobs I, Laird PW.|
|Nat Genet. 2007 Feb;39(2):157-8. Epub 2006 Dec 31.|
|Activation and transposition of endogenous retroviral elements in hypomethylation induced tumors in mice.|
|Howard G, Eiges R, Gaudet F, Jaenisch R, Eden A.|
|Oncogene. 2008 Jan 10;27(3):404-8. Epub 2007 Jul 9.|
|Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells.|
|Ball MP, Li JB, Gao Y, Lee JH, LeProust EM, Park IH, Xie B, Daley GQ, Church GM.|
|Nat Biotechnol. 2009 Apr;27(4):361-8. doi: 10.1038/nbt.1533. Epub 2009 Mar 29.|
|An operational definition of epigenetics.|
|Berger SL, Kouzarides T, Shiekhattar R, Shilatifard A.|
|Genes Dev. 2009 Apr 1;23(7):781-3. doi: 10.1101/gad.1787609.|
|A new class of quinoline-based DNA hypomethylating agents reactivates tumor suppressor genes by blocking DNA methyltransferase 1 activity and inducing its degradation.|
|Datta J, Ghoshal K, Denny WA, Gamage SA, Brooke DG, Phiasivongsa P, Redkar S, Jacob ST.|
|Cancer Res. 2009 May 15;69(10):4277-85. doi: 10.1158/0008-5472.CAN-08-3669. Epub 2009 May 5.|
|Prostate cancer detected by methylated gene markers in histopathologically cancer-negative tissues from men with subsequent positive biopsies.|
|Troyer DA, Lucia MS, de Bruine AP, Mendez-Meza R, Baldewijns MM, Dunscomb N, Van Engeland M, McAskill T, Bierau K, Louwagie J, Bigley JW.|
|Cancer Epidemiol Biomarkers Prev. 2009 Oct;18(10):2717-22. doi: 10.1158/1055-9965.EPI-09-0068. Epub 2009 Sep 15.|
|Epigenetic silencing of the p16(INK4a) tumor suppressor is associated with loss of CTCF binding and a chromatin boundary.|
|Witcher M, Emerson BM.|
|Mol Cell. 2009 May 15;34(3):271-84. doi: 10.1016/j.molcel.2009.04.001.|
|S110, a 5-Aza-2'-deoxycytidine-containing dinucleotide, is an effective DNA methylation inhibitor in vivo and can reduce tumor growth.|
|Chuang JC, Warner SL, Vollmer D, Vankayalapati H, Redkar S, Bearss DJ, Qiu X, Yoo CB, Jones PA.|
|Mol Cancer Ther. 2010 May;9(5):1443-50. doi: 10.1158/1535-7163.MCT-09-1048. Epub 2010 May 4.|
|Consolidation of the cancer genome into domains of repressive chromatin by long-range epigenetic silencing (LRES) reduces transcriptional plasticity.|
|Coolen MW, Stirzaker C, Song JZ, Statham AL, Kassir Z, Moreno CS, Young AN, Varma V, Speed TP, Cowley M, Lacaze P, Kaplan W, Robinson MD, Clark SJ.|
|Nat Cell Biol. 2010 Mar;12(3):235-46. doi: 10.1038/ncb2023. Epub 2010 Feb 21.|
|Genome architecture marked by retrotransposons modulates predisposition to DNA methylation in cancer.|
|Estecio MR, Gallegos J, Vallot C, Castoro RJ, Chung W, Maegawa S, Oki Y, Kondo Y, Jelinek J, Shen L, Hartung H, Aplan PD, Czerniak BA, Liang S, Issa JP.|
|Genome Res. 2010 Oct;20(10):1369-82. doi: 10.1101/gr.107318.110. Epub 2010 Aug 17.|
|A decade of exploring the cancer epigenome - biological and translational implications.|
|Baylin SB, Jones PA.|
|Nat Rev Cancer. 2011 Sep 23;11(10):726-34. doi: 10.1038/nrc3130. (REVIEW)|
|C5-DNA methyltransferase inhibitors: from screening to effects on zebrafish embryo development.|
|Ceccaldi A, Rajavelu A, Champion C, Rampon C, Jurkowska R, Jankevicius G, Senamaud-Beaufort C, Ponger L, Gagey N, Ali HD, Tost J, Vriz S, Ros S, Dauzonne D, Jeltsch A, Guianvarc'h D, Arimondo PB.|
|Chembiochem. 2011 Jun 14;12(9):1337-45. doi: 10.1002/cbic.201100130. Epub 2011 Jun 1.|
|Combination epigenetic therapy has efficacy in patients with refractory advanced non-small cell lung cancer.|
|Juergens RA, Wrangle J, Vendetti FP, Murphy SC, Zhao M, Coleman B, Sebree R, Rodgers K, Hooker CM, Franco N, Lee B, Tsai S, Delgado IE, Rudek MA, Belinsky SA, Herman JG, Baylin SB, Brock MV, Rudin CM.|
|Cancer Discov. 2011 Dec;1(7):598-607. doi: 10.1158/2159-8290.CD-11-0214. Epub 2011 Nov 9.|
|DNA methyltransferase inhibitors in cancer: a chemical and therapeutic patent overview and selected clinical studies.|
|Fahy J, Jeltsch A, Arimondo PB.|
|Expert Opin Ther Pat. 2012 Dec;22(12):1427-42. doi: 10.1517/13543776.2012.729579. Epub 2012 Oct 3. (REVIEW)|
|5-Methylcytosine DNA demethylation: more than losing a methyl group.|
|Franchini DM, Schmitz KM, Petersen-Mahrt SK.|
|Annu Rev Genet. 2012;46:419-41. doi: 10.1146/annurev-genet-110711-155451. Epub 2012 Sep 4. (REVIEW)|
|Nucleic acid modifications with epigenetic significance.|
|Fu Y, He C.|
|Curr Opin Chem Biol. 2012 Dec;16(5-6):516-24. doi: 10.1016/j.cbpa.2012.10.002. Epub 2012 Oct 22. (REVIEW)|
|DNA methylation inhibitors in cancer: recent and future approaches.|
|Gros C, Fahy J, Halby L, Dufau I, Erdmann A, Gregoire JM, Ausseil F, Vispe S, Arimondo PB.|
|Biochimie. 2012 Nov;94(11):2280-96. doi: 10.1016/j.biochi.2012.07.025. Epub 2012 Aug 11. (REVIEW)|
|Rapid synthesis of new DNMT inhibitors derivatives of procainamide.|
|Halby L, Champion C, Senamaud-Beaufort C, Ajjan S, Drujon T, Rajavelu A, Ceccaldi A, Jurkowska R, Lequin O, Nelson WG, Guy A, Jeltsch A, Guianvarc'h D, Ferroud C, Arimondo PB.|
|Chembiochem. 2012 Jan 2;13(1):157-65. doi: 10.1002/cbic.201100522. Epub 2011 Dec 14.|
|The future of epigenetic therapy in solid tumours--lessons from the past.|
|Azad N, Zahnow CA, Rudin CM, Baylin SB.|
|Nat Rev Clin Oncol. 2013 May;10(5):256-66. doi: 10.1038/nrclinonc.2013.42. Epub 2013 Apr 2. (REVIEW)|
|Regional activation of the cancer genome by long-range epigenetic remodeling.|
|Bert SA, Robinson MD, Strbenac D, Statham AL, Song JZ, Hulf T, Sutherland RL, Coolen MW, Stirzaker C, Clark SJ.|
|Cancer Cell. 2013 Jan 14;23(1):9-22. doi: 10.1016/j.ccr.2012.11.006. Epub 2012 Dec 13.|
|Identification of novel inhibitors of DNA methylation by screening of a chemical library.|
|Ceccaldi A, Rajavelu A, Ragozin S, Senamaud-Beaufort C, Bashtrykov P, Testa N, Dali-Ali H, Maulay-Bailly C, Amand S, Guianvarc'h D, Jeltsch A, Arimondo PB.|
|ACS Chem Biol. 2013 Mar 15;8(3):543-8. doi: 10.1021/cb300565z. Epub 2013 Jan 11.|
|Mechanism-based epigenetic chemosensitization therapy of diffuse large B-cell lymphoma.|
|Clozel T, Yang S, Elstrom RL, Tam W, Martin P, Kormaksson M, Banerjee S, Vasanthakumar A, Culjkovic B, Scott DW, Wyman S, Leser M, Shaknovich R, Chadburn A, Tabbo F, Godley LA, Gascoyne RD, Borden KL, Inghirami G, Leonard JP, Melnick A, Cerchietti L.|
|Cancer Discov. 2013 Sep;3(9):1002-19. doi: 10.1158/2159-8290.CD-13-0117. Epub 2013 Aug 16.|
|DNMT1-interacting RNAs block gene-specific DNA methylation.|
|Di Ruscio A, Ebralidze AK, Benoukraf T, Amabile G, Goff LA, Terragni J, Figueroa ME, De Figueiredo Pontes LL, Alberich-Jorda M, Zhang P, Wu M, D'Alo F, Melnick A, Leone G, Ebralidze KK, Pradhan S, Rinn JL, Tenen DG.|
|Nature. 2013 Nov 21;503(7476):371-6. doi: 10.1038/nature12598. Epub 2013 Oct 9.|
|Identification of DNMT1 selective antagonists using a novel scintillation proximity assay.|
|Kilgore JA, Du X, Melito L, Wei S, Wang C, Chin HG, Posner B, Pradhan S, Ready JM, Williams NS.|
|J Biol Chem. 2013 Jul 5;288(27):19673-84. doi: 10.1074/jbc.M112.443895. Epub 2013 May 13.|
|Highly synergistic effect of sequential treatment with epigenetic and anticancer drugs to overcome drug resistance in breast cancer cells is mediated via activation of p21 gene expression leading to G2/M cycle arrest.|
|Vijayaraghavalu S, Dermawan JK, Cheriyath V, Labhasetwar V.|
|Mol Pharm. 2013 Jan 7;10(1):337-52. doi: 10.1021/mp3004622. Epub 2012 Dec 24.|
|Alterations of immune response of Non-Small Cell Lung Cancer with Azacytidine.|
|Wrangle J, Wang W, Koch A, Easwaran H, Mohammad HP, Vendetti F, Vancriekinge W, Demeyer T, Du Z, Parsana P, Rodgers K, Yen RW, Zahnow CA, Taube JM, Brahmer JR, Tykodi SS, Easton K, Carvajal RD, Jones PA, Laird PW, Weisenberger DJ, Tsai S, Juergens RA, Topalian SL, Rudin CM, Brock MV, Pardoll D, Baylin SB.|
|Oncotarget. 2013 Nov;4(11):2067-79.|
|Identification of kazinol q, a natural product from formosan plants, as an inhibitor of DNA methyltransferase.|
|Weng JR, Lai IL, Yang HC, Lin CN, Bai LY.|
|Phytother Res. 2014 Jan;28(1):49-54. doi: 10.1002/ptr.4955. Epub 2013 Feb 28.|
|Written||2013-11||Céline Moison, Anne-Laure Guieysse-Peugeot, Paola B Arimondo|
|Fabre, USR3388, Epigenetic Targeting of Cancer (ETaC), Toulouse, France, MNHN CNRS UMR7196, INSERM U565, Paris, France, Universite Pierre et Marie Curie, Paris, France (CM); MNHN CNRS UMR7196, INSERM U565, Paris, France (ALGP); CNRS-Pierre Fabre, USR3388, Epigenetic Targeting of Cancer (ETaC), Toulouse, France (PBA)|
|This paper should be referenced as such :|
|Moison, C ; Guieysse-Peugeot, AL ; Arimondo, PB|
|DNA methylation in cancer|
|Atlas Genet Cytogenet Oncol Haematol. 2014;18(4):285-292.|
|Free journal version : [ pdf ] [ DOI ]|
|On line version : http://AtlasGeneticsOncology.org/Deep/DNAMethylationID20127.htm|
|© Atlas of Genetics and Cytogenetics in Oncology and Haematology||indexed on : Mon Jul 8 16:01:27 CEST 2019|
For comments and suggestions or contributions, please contact us