Atlas of Genetics and Cytogenetics in Oncology and Haematology

Home   Genes    Leukemias    Solid Tumors    Cancer-Prone    Deep Insight    Case Reports    Journals   Portal    Teaching   
X Y 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 NA

JAK2 mutations in myeloproliferative neoplasms

Naseema Gangat, Ayalew Tefferi

Division of Hematology, Mayo Clinic, Rochester, MN, United States

Correspondence: Ayalew Tefferi, MD Mayo Clinic 200 First Street SW Rochester MN 55905
Tel: +1 507 284 2479
Fax: +1 507 266 4972
tefferi.ayalew@mayo.edu

 

September 2008

 

 

 

 

 

Introduction

Myeloproliferative neoplasms (MPN) are clonal disorders of hematopoietic stem cells that clinically manifest as overproduction of cells that contribute to the myeloid lineage. These include the "classical MPN's" chronic myeloid leukemia (CML), polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF) (Vardiman et al., 2002), as well as "atypical MPN's"; chronic eosinophilic leukemia (James et al., 2005), chronic myelomonocytic leukemia (CMML), and systemic mastocytosis (SM) (Tefferi and Gilliland, 2006).
In 1951, CML, PV, ET and PMF were recognized to have significant overlap in both clinical and biological features and were felt to be related diseases (Dameshek, 1951). In 1960, CML was recognized as a distinct entity after discovery of the Philadelphia chromosome (Nowell and Hungerford, 1960).
In the early 1980s, Fialkow analyzed X chromosome inactivation patterns in women with PV, ET, PMF or CML carrying a polymorphic variant of the gene for glucose-6-phosphate dehydrogenase. Based on the observations, they established that all four diseases were clonal stem cell disorders (Adamson et al., 1976; Fialkow et al., 1977; Fialkow et al., 1981). Recently in 2005, several independent groups identified a somatic mutation involving a protein tyrosine kinase in patients with PV, ET and PMF (Baxter et al., 2005; James et al., 2005; Kralovics et al., 2005; Levine et al., 2005). This was a JAK2 point mutation at codon 617, subsequently named JAK2V617F which results from a G -->T transversion at nucleotide 1849 in exon 14 of the JAK2 gene, the consequence of which is substitution of valine by phenylalanine at codon 617. The mutation is present in hematopoietic cells from affected individuals, but not in the germline, suggesting that this mutation is acquired as a somatic disease allele in the hematopoietic compartment. This mutation is observed in 95% of patients with PV, and 50% of patients with ET and PMF (Tefferi and Gilliland, 2005). In the last 2 years, additional JAK2 mutations have been reported and have shown to induce PV-(JAK2)-like phenotype in mice (Scott et al., 2007b).


JAK structure and function

The acronym JAK stands for Just Another Kinase, which refers to the discovery of multiple tyrosine kinase family members. Janus kinases (JAKs) are named after the Roman god with two faces because they contain two symmetrical kinase-like domains; the C-terminal JAK homology 1 (JH1) and the immediately adjacent JH2 or pseudokinase domain (Saharinen and Silvennoinen, 2002; Feener et al., 2004). Valine 617 is within the JH2 domain. There are four mammalian members of the JAK family of receptor-associated tyrosine kinases: JAK1, JAK2, JAK3 and tyrosine kinase 2 (TYK2). They comprise of seven homologous JH domains organized into four regions; kinase (JH1), pseudo-kinase (JH2), FERM (the N-terminal JH7, JH6, JH5 and part of JH4) and SH2-like (JH3 and part of JH4) (Schindler et al., 2007). The carboxy-terminal portion of these molecules includes a distinctive kinase domain (JH1) which is catalytically active and a catalytically inactive pseudo kinase domain (JH2) which is felt to regulate the activity of JH1. The amino-terminal JH domains, JH3-JH7, constitute a FERM (four-point-one, ezrin, radixin, moesin) domain and mediate association with receptors (Funakoshi-Tago et al., 2006).
In humans, JAK1 is located on chromosome 1p31.3, JAK2 on 9p24, JAK3 on 19p13.1 and TYK2 on 19p13.2. Each one of these genes ranges in size from 120 to 130 kDa containing 20 - 25 exons. Expression is ubiquitous for JAK1, JAK2 and TYK2 but restricted to hematopoietic cells for JAK3.


Typically, JAK kinases function through their interaction with cytokine receptors that lack intrinsic kinase activity. Ligand binding (e.g. erythropoietin, thrombopoietin) to the appropriate cytokine receptor (type 1 or type 2 cytokine receptors; e.g. EpoR, MPL) results in juxtaposition of JAKs followed by JAK kinase phosphorylation and activation, cytokine receptor phosphorylation and creates a docking site for the recruitment and activation of signal transducers and activators of transcription (STATs) (Mertens and Darnell, 2007). Following phosphorylation, activated STATs dimerize and translocate into the nucleus to induce target gene transcription. This entire process is tightly regulated at multiple levels by protein tyrosine phosphatases, suppressors of cytokine signaling (SOCS) and protein inhibitors of activated STAT (Starr and Hilton, 1999; Sasaki et al., 2000; Stofega et al., 2000).
JAK2 was first identified in 1993 (Harpur et al., 1992) and was found to be an essential mediator for erythropoietin signaling (Witthuhn et al., 1993). The JAK2 gene is located on chromosome 9p24. Genetic deletion of JAK2 results in embryonic death due to lack of definitive erythropoiesis, and JAK2- deficient hematopoietic progenitors do not respond to erythropoietin stimulation, suggesting that JAK2 is the only JAK kinase responsible for erythropoietin receptor signaling (Parganas et al., 1998).


JAK2V617F mutations in PV, ET and PMF

In 2005, several independent groups identified a recurrent mutation in the JAK2 tyrosine kinase in most patients with PV, ET or PMF (Baxter et al., 2005; James et al., 2005; Kralovics et al., 2005; Levine et al., 2005). Subsequently, the mutation was also described in other myeloid neoplasms (Steensma et al., 2005). JAK2V617F is an exon 14 G to T somatic mutation. This mutation is a guanine-to-thymidine substitution, which results in a substitution of valine for phenylalanine at codon 617 within the JH2 domain of JAK2 (JAK2V617F). The JH2 domain is believed to be auto-inhibitory (Saharinen and Silvennoinen, 2002), and valine 617 plays an important role in JAK2 kinase auto-inhibition (Lindauer et al., 2001). Thus, the valine to phenylalanine substitution at codon 617 results in constitutive kinase activity resulting in a gain-of-function mutation (Ihle and Gilliland, 2007). Studies have shown that expression of JAK2V617F results in transformation of Ba/F3 cells to IL-3 independent growth, unlike wild-type JAK2 (James et al., 2005). Similarly, coexpression of JAK2V617F and erythropoietin receptor, thrombopoietin receptor (MPL), or granulocyte-macrophage colony stimulating factor (GM-CSF) receptor (all homodimeric Type 1 cytokine receptors) result in cytokine independent growth and activation of signal transduction (Lu et al., 2005). In addition, expression of JAK2V617F, results in constitutive activation of downstream signaling pathways including the JAK-STAT, PI3K/AKT and MAPK/ERK pathways (James et al., 2005; Kralovics et al., 2005; Levine et al., 2005).
The JAK2V617F mutation has been reported in over 95% of patients with PV, 50% of patients with ET or PMF, 20% in certain other MPNs including refractory anemia with ring sideroblasts and thrombocytosis (RARS-T), and less than 5% in AML or MDS (Renneville et al., 2006; Steensma et al., 2006; Verstovsek et al., 2006). JAK2V617F is a somatically acquired mutation and a subset of patients with PV are homozygous for the JAK2V617F allele as a result of mitotic recombination and duplication of the mutant allele, known as uniparental disomy. Uniparental disomy of chromosomal locus 9p24, including JAK2, had previously been noted in PV (Kralovics et al., 2002), and later the JAK2V617F allele was identified through analysis of the minimal region of uniparental disomy (Kralovics et al., 2005). It has been shown that most patients with PV possess JAK2V617F homozygous mutant erythroid progenitors, while most patients with ET possess only heterozygous and wild-type erythroid colonies (Scott et al., 2006). These observations suggest that mitotic recombination and JAK2V617F homozygosity is an early genetic event in the development of PV, but not ET.
In humans, JAK2V617F mutation occurs at the stem cell level and is present in hematopoietic stem cell progenitors (Baxter et al., 2005; Jamieson et al., 2006). It is believed to be myeloid lineage specific because it is present in erythroid and granulocyte-macrophage progenitors (Baxter et al., 2005; James et al., 2005). However, some reports have suggested JAK2V617F clonal involvement of B (Ishii et al., 2006), T (Ishii et al., 2006), and NK (Bellanne-Chantelot et al., 2006) lymphocytes. These observations confirm the stem cell nature of JAK2V617F MPN's.
In retroviral transplant mouse models JAK2V617F induces a PV-like phenotype: erythrocytosis, low serum erythropoietin level, splenomegaly due to extramedullary hematopoiesis, leukocytosis, megakaryocytic hyperplasia and ultimately evolution to myelofibrosis (Lacout et al., 2006; Wernig et al., 2006). It has also been shown that in JAK2V617F transgenic mice, manipulation of mutant gene expression results in either an ET (lower expression compared with wild-type allele) or PV phenotype with (equal expression) or without (higher expression) thrombocytosis (Tiedt et al., 2008). Based on the above experiments, we observe that mutant allele burden in patients with ET is significantly lower than that seen in patients with either PV or PMF (Kittur et al., 2007; (Tefferi et al., 2007; Vannucchi et al., 2007b; Tefferi et al., 2008). In PV, a higher allele burden is the result of JAK2V617F homozygosity.
Although JAK2V617F is central to the pathogenesis of PV, ET, PMF, the presence of the same allele in three clinically distinct MPN's suggests that there might be additional inherited or acquired genetic predisposition. A familial tendency has been reported in 72 families with at least two members with MPN's that were tested for JAK2V617F (Bellanne-Chantelot et al., 2006). The presence of JAK2V617F in these families ranged from some families in which all members carried the mutation, to some in which none of the members with MPN had the JAK2V617F mutation. This pattern is consistent with a two-hit hypothesis, with an inherited genetic predisposition to MPN (Pardanani et al., 2006a).
There is further evidence to suggest that other somatic alleles are involved along with JAK2V617F in the development of MPN's. It is noted that in a subset of JAK2V617F positive MPN who transform to AML, the leukemic blasts are JAK2V617F mutation negative (Theocharides et al., 2007).
Other reported JAK2 exon 14 mutations include D620E (PV, MPN, unclassifiable), E627E (MPN, unclassifiable), C616Y (PV), V617F from c.1848_1849delinsCT (post-ET MF) and V617F/C618R from c.1849 - 1852GTCT > TTTC (PV) (Grunebach et al., 2006; Schnittger et al., 2006; Wong et al., 2007; Zhang et al., 2007).


JAK2 exon 12 mutations

A small proportion of patients with PV are JAK2V617F negative when tested by sensitive allele-specific assays (Jones et al., 2005). However, in 2007, Scott and colleagues identified a set of JAK2 exon 12 mutations in JAK2V617F-negative patients with PV (Scott et al., 2007b). The majority of these cases were found to harbor 1 of 4 exon 12 JAK2 mutant alleles: N542-E543del, F537-K539delinsL, a point mutation that results in substitution of lysine for leucine at codon 539 (K539L), and H538QK539L. All four exon 12 mutant alleles induced cytokine-independent/hypersensitive proliferation in erythropoietin receptor-expressing cell lines and constitutive activation of JAK-STAT signalling (Scott et al., 2007b). In addition, JAK2K539L induced a PV phenotype in a murine transplant model. Unlike JAK2V617F, JAK2 exon 12 mutations are only observed in JAK2V617F- negative PV (i.e. approximately 5% of all PV cases) (Scott et al., 2007a; Butcher et al., 2008), and are specific to patients with isolated erythrocytosis without concomitant leukocytosis or thrombocytosis.
Several other exon 12 mutation variants have been identified; R541 - E543delinsK, I540 - E543delinsMK, V536-I546dup11, F537-I546dup10 + 547L and E543-D544del (Percy et al., 2007; Butcher et al., 2008; Pietra et al., 2008).


MPL mutations

Approximately 50% of ET and PMF patients are JAK2V617F negative. This led Pikman and colleagues (Pikman et al., 2006) to study whether other genes in the JAK-STAT signaling pathway might be mutated in JAK2V617F negative ET and PMF. This led to the identification of mutations of the thrombopoietin receptor (MPL), which substitute either leucine or lysine by tryptophan at codon 515. These mutations occur in 10% of JAK2V617F negative PMF and in 2% of JAK2V617F negative ET, but not observed in PV or other myeloid malignancies (Pardanani et al., 2006b).


Clinical correlates of JAK2 mutations in PV, ET and PMF

The prognostic significance of the JAK2V617F mutation has not been precisely delineated. In ET, the presence of JAK2V617F has been associated with advanced age, higher hemoglobin level, higher leukocyte counts and lower platelet counts (Antonioli et al., 2005; Campbell et al., 2005; Wolanskyj et al., 2005; Kittur et al., 2007). In addition in mutation-positive patients with ET, JAK2V617F allele burden has been directly correlated with leukocyte count, platelet count and the presence of palpable splenomegaly (Kittur et al., 2007; Vannucchi et al., 2007b). Although JAK2V617F mutant and wild- type patients differ in regards to laboratory parameters and clinical features, there were no differences in overall survival, or myelofibrotic and leukemic transformations. In PV, JAK2V617F homozygous patients were noted to have a higher hemoglobin level, higher leukocyte count, lower platelet count, and an increased incidence of pruritus but not an increased risk of thrombosis (Vannucchi et al., 2007b). A similar set of correlations were made for higher mutant allele burden in PV, measured by quantitative assays (Tefferi et al., 2007; Vannucchi et al., 2007a). In PMF, the presence of JAK2V617F was associated with older age at diagnosis, higher leukocyte count and presence of pruritus (Tefferi et al., 2005). Furthermore, JAK2V617F “homozygous” PMF patients displayed even higher leukocyte count and spleen size (Barosi et al., 2007). In PMF, the presence of the JAK2V617F mutation is associated with poorer overall survival (Campbell et al., 2006). However, these data suggest that JAK2V617F mutational status may have prognostic significance in PV, ET and PMF, but additional studies of large patient cohorts are needed to confirm these findings.
The prevalence of JAK2 exon 12 is too low to enable accurate assessment of prognostic impact. Nevertheless, JAK2 exon 12 mutations have been associated with a PV phenotype that is more likely to present with isolated erythrocytosis (Scott et al., 2007b; Pietra et al., 2008).

Conclusion

The discovery of the JAK2V617F mutation in 2005 has been pivotal to our understanding of the pathogenesis of PV, ET and PMF. The subsequent discovery in 2007 of the JAK2 exon 12 mutations in JAK2V617F negative PV suggests that activation of the JAK-STAT pathway is important in the pathogenesis of JAK2V617F-negative MPN. Understanding the molecular pathogenesis of diseases, such as bcr-abl in CML, and now JAK2V617F in PV, ET, and PMF has led to the development of small molecule inhibitors of JAK2.
Accordingly, a number of anti-JAK2 small molecule drugs have been tested in preclinical models and some have been introduced into clinical trials (Pardanani et al., 2007; Lasho et al., 2008; Pardanani, 2008). Preliminary results from currently ongoing JAK2 inhibitor drug trials (INCB018424, XL019) suggest remarkable activity in alleviating symptomatic splenomegaly and constitutional symptoms in patients with primary or post-PV/ET myelofibrosis (Verstovsek, 2007a; Verstovsek, 2007b). Despite the discovery of JAK2 and MPL mutations there are several unanswered questions regarding the etiology of PV, ET and PMF; how does a single disease allele contribute to three distinct clinical disorders? Will JAK2 inhibitor therapy offer clinical benefit to patients with PV, ET and PMF?

Bibliography

Some speculations on the myeloproliferative syndromes.
Dameshek W.
Blood. 1951 Apr;6(4):372-5.
PMID 14820991
 
Chromosome studies on normal and leukemic human leukocytes.
NOWELL PC, HUNGERFORD DA.
J Natl Cancer Inst. 1960 Jul;25: 85-109.
PMID 14427847
 
Polycythemia vera: stem-cell and probable clonal origin of the disease.
Adamson JW, Fialkow PJ, Murphy S, Prchal JF, Steinmann L.
N Engl J Med. 1976 Oct 21;295(17): 913-6.
PMID 967201
 
Chronic myelocytic leukemia: clonal origin in a stem cell common to the granulocyte, erythrocyte, platelet and monocyte/macrophage.
Fialkow PJ, Jacobson RJ, Papayannopoulou T.
Am J Med. 1977 Jul;63(1): 125-30.
PMID 267431
 
Evidence that essential thrombocythemia is a clonal disorder with origin in a multipotent stem cell.
Fialkow PJ, Faguet GB, Jacobson RJ, Vaidya K, Murphy S.
Blood. 1981 Nov;58(5):916-9.
PMID 7296002
 
JAK2, a third member of the JAK family of protein tyrosine kinases.
Harpur AG, Andres AC, Ziemiecki A, Aston RR, Wilks AF.
Oncogene. 1992 Jul;7(7):1347-53.
PMID 1620548
 
JAK2 associates with the erythropoietin receptor and is tyrosine phosphorylated and activated following stimulation with erythropoietin.
Witthuhn BA, Quelle FW, Silvennoinen O, Yi T, Tang B, Miura O, Ihle JN.
Cell. 1993 Jul 30;74(2):227-36.
PMID 8343951
 
Jak2 is essential for signaling through a variety of cytokine receptors.
Parganas E, Wang D, Stravopodis D, Topham DJ, Marine JC, Teglund S, Vanin EF, Bodner S, Colamonici OR, van Deursen JM, Grosveld G, Ihle JN.
Cell. 1998 May 1;93(3):385-95.
PMID 9590173
 
Negative regulation of the JAK/STAT pathway.
Starr R, Hilton DJ.
Bioessays. 1999 Jan;21(1):47-52.
PMID 10070253
 
CIS3/SOCS-3 suppresses erythropoietin (EPO) signaling by binding the EPO receptor and JAK2.
Sasaki A, Yasukawa H, Shouda T, Kitamura T, Dikic I, Yoshimura A.
J Biol Chem. 2000 Sep 22;275(38):29338-47.
PMID 10882725
 
Mutation of the SHP-2 binding site in growth hormone (GH) receptor prolongs GH-promoted tyrosyl phosphorylation of GH receptor, JAK2, and STAT5B.
Stofega MR, Herrington J, Billestrup N, Carter-Su C.
Mol Endocrinol. 2000 Sep;14(9):1338-50.
PMID 10976913
 
Prediction of the structure of human Janus kinase 2 (JAK2) comprising the two carboxy-terminal domains reveals a mechanism for autoregulation.
Lindauer K, Loerting T, Liedl KR, Kroemer RT.
Protein Eng. 2001 Jan;14(1):27-37.
PMID 11287676
 
Acquired uniparental disomy of chromosome 9p is a frequent stem cell defect in polycythemia vera.
Kralovics R, Guan Y, Prchal JT.
Exp Hematol. 2002 Mar;30(3):229-36.
PMID 11882360
 
The pseudokinase domain is required for suppression of basal activity of Jak2 and Jak3 tyrosine kinases and for cytokine-inducible activation of signal transduction.
Saharinen P, Silvennoinen O.
J Biol Chem. 2002 Dec 6;277(49):47954-63.
PMID 12351625
 
The World Health Organization (WHO) classification of the myeloid neoplasms.
Vardiman JW, Harris NL, Brunning RD.
Blood. 2002 Oct 1;100(7):2292-302.
PMID 12239137
 
Tyrosine phosphorylation of Jak2 in the JH2 domain inhibits cytokine signaling.
Feener EP, Rosario F, Dunn SL, Stancheva Z, Myers MG Jr.
Mol Cell Biol. 2004 Jun;24(11):4968-78.
PMID 15143188
 
Clinical implications of the JAK2 V617F mutation in essential thrombocythemia.
Antonioli E, Guglielmelli P, Pancrazzi A, Bogani C, Verrucci M, Ponziani V, Longo G, Bosi A, Vannucchi AM.
Leukemia. 2005 Oct;19(10):1847-9
PMID 16079890
 
Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders.
Baxter EJ, Scott LM, Campbell PJ, East C, Fourouclas N, Swanton S, Vassiliou GS, Bench AJ, Boyd EM, Curtin N, Scott MA, Erber WN, Green AR.
Lancet. 2005 Mar 19-25;365(9464):1054-61.
PMID 15781101
 
Definition of subtypes of essential thrombocythaemia and relation to polycythaemia vera based on JAK2 V617F mutation status: a prospective study.
Campbell PJ, Scott LM, Buck G, Wheatley K, East CL, Marsden JT, Duffy A, Boyd EM, Bench AJ, Scott MA, Vassiliou GS, Milligan DW, Smith SR, Erber WN, Bareford D, Wilkins BS, Reilly JT, Harrison CN, Green AR; United Kingdom Myeloproliferative Disorders Study Group; Medical Research Council Adult Leukaemia Working Party; Australasian Leukaemia and Lymphoma Group.
Lancet. 2005 Dec 3;366(9501):1945-53.
PMID 16325696
 
A unique clonal JAK2 mutation leading to constitutive signalling causes polycythaemia vera.
James C, Ugo V, Le Couedic JP, Staerk J, Delhommeau F, Lacout C, Garçon L, Raslova H, Berger R, Bennaceur-Griscelli A, Villeval JL, Constantinescu SN, Casadevall N, Vainchenker W.
Nature. 2005 Apr 28;434(7037):1144-8.
PMID 15793561
 
Widespread occurrence of the JAK2 V617F mutation in chronic myeloproliferative disorders.
Jones AV, Kreil S, Zoi K, Waghorn K, Curtis C, Zhang L, Score J, Seear R, Chase AJ, Grand FH, White H, Zoi C, Loukopoulos D, Terpos E, Vervessou EC, Schultheis B, Emig M, Ernst T, Lengfelder E, Hehlmann R, Hochhaus A, Oscier D, Silver RT, Reiter A, Cross NC.
Blood. 2005 Sep 15;106(6):2162-8.
PMID 15920007
 
A gain-of-function mutation of JAK2 in myeloproliferative disorders.
Kralovics R, Passamonti F, Buser AS, Teo SS, Tiedt R, Passweg JR, Tichelli A, Cazzola M, Skoda RC.
N Engl J Med. 2005 Apr 28;352(17):1779-90
PMID 15858187
 
Activating mutation in the tyrosine kinase JAK2 in polycythemia vera, essential thrombocythemia, and myeloid metaplasia with myelofibrosis.
Levine RL, Wadleigh M, Cools J, Ebert BL, Wernig G, Huntly BJ, Boggon TJ, Wlodarska I, Clark JJ, Moore S, Adelsperger J, Koo S, Lee JC, Gabriel S, Mercher T, D'Andrea A, Frohling S, Dohner K, Marynen P, Vandenberghe P, Mesa RA, Tefferi A, Griffin JD, Eck MJ, Sellers WR, Meyerson M, Golub TR, Lee SJ, Gilliland DG.
Cancer Cell. 2005 Apr;7(4):387-97.
PMID 15837627
 
Expression of a homodimeric type I cytokine receptor is required for JAK2V617F-mediated transformation.
Lu X, Levine R, Tong W, Wernig G, Pikman Y, Zarnegar S, Gilliland DG, Lodish H.
Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):18962-7.
PMID 16365288
 
The JAK2 V617F activating tyrosine kinase mutation is an infrequent event in both "atypical" myeloproliferative disorders and myelodysplastic syndromes.
Steensma DP, Dewald GW, Lasho TL, Powell HL, McClure RF, Levine RL, Gilliland DG, Tefferi A.
Blood. 2005 Aug 15;106(4):1207-9.
PMID 15860661
 
JAK2 in myeloproliferative disorders is not just another kinase.
Tefferi A, Gilliland DG.
Cell Cycle. 2005 Aug;4(8):1053-6.
PMID 15970705
 
The JAK2(V617F) tyrosine kinase mutation in myelofibrosis with myeloid metaplasia: lineage specificity and clinical correlates.
Tefferi A, Lasho TL, Schwager SM, Steensma DP, Mesa RA, Li CY, Wadleigh M, Gary Gilliland D.
Br J Haematol. 2005 Nov;131(3):320-8.
PMID 16225651
 
JAK2 mutation in essential thrombocythaemia: clinical associations and long-term prognostic relevance.
Wolanskyj AP, Lasho TL, Schwager SM, McClure RF, Wadleigh M, Lee SJ, Gilliland DG, Tefferi A.
Br J Haematol. 2005 Oct;131(2):208-13.
PMID 16197451
 
Genetic and clinical implications of the Val617Phe JAK2 mutation in 72 families with myeloproliferative disorders.
Bellanne-Chantelot C, Chaumarel I, Labopin M, Bellanger F, Barbu V, De Toma C, Delhommeau F, Casadevall N, Vainchenker W, Thomas G, Najman A.
Blood. 2006 Jul 1;108(1):346-52.
PMID 16537803
 
V617F mutation in JAK2 is associated with poorer survival in idiopathic myelofibrosis.
Campbell PJ, Griesshammer M, Dohner K, Dohner H, Kusec R, Hasselbalch HC, Larsen TS, Pallisgaard N, Giraudier S, Le Bousse-Kerdiles MC, Desterke C, Guerton B, Dupriez B, Bordessoule D, Fenaux P, Kiladjian JJ, Viallard JF, Briere J, Harrison CN, Green AR, Reilly JT.
Blood. 2006 Mar 1;107(5):2098-100.
PMID 16293597
 
Receptor specific downregulation of cytokine signaling by autophosphorylation in the FERM domain of Jak2.
Funakoshi-Tago M, Pelletier S, Matsuda T, Parganas E, Ihle JN.
Embo J. 2006 Oct 18;25(20):4763-72
PMID 17024180
 
Detection of a new JAK2 D620E mutation in addition to V617F in a patient with polycythemia vera.
Grunebach F, Bross-Bach U, Kanz L, Brossart P.
Leukemia. 2006 Dec;20(12):2210-1.
PMID 17008888
 
Involvement of various hematopoietic-cell lineages by the JAK2V617F mutation in polycythemia vera.
Ishii T, Bruno E, Hoffman R, Xu M.
Blood. 2006 Nov 1;108(9):3128-34.
PMID 16757685
 
The JAK2 V617F mutation occurs in hematopoietic stem cells in polycythemia vera and predisposes toward erythroid differentiation.
Jamieson CH, Gotlib J, Durocher JA, Chao MP, Mariappan MR, Lay M, Jones C, Zehnder JL, Lilleberg SL, Weissman IL.
Proc Natl Acad Sci U S A. 2006 Apr 18;103(16):6224-9.
PMID 16603627
 
JAK2V617F expression in murine hematopoietic cells leads to MPD mimicking human PV with secondary myelofibrosis.
Lacout C, Pisani DF, Tulliez M, Gachelin FM, Vainchenker W, Villeval JL.
Blood. 2006 Sep 1;108(5):1652-60.
PMID 16670266
 
Discordant distribution of JAK2V617F mutation in siblings with familial myeloproliferative disorders.
Pardanani A, Lasho T, McClure R, Lacy M, Tefferi A.
Blood. 2006a Jun 1;107(11):4572-3.
PMID 16717134
 
MPL515 mutations in myeloproliferative and other myeloid disorders: a study of 1182 patients.
Pardanani AD, Levine RL, Lasho T, Pikman Y, Mesa RA, Wadleigh M, Steensma DP, Elliott MA, Wolanskyj AP, Hogan WJ, McClure RF, Litzow MR, Gilliland DG, Tefferi A.
Blood. 2006b Nov 15;108(10):3472-6.
PMID 16868251
 
MPLW515L is a novel somatic activating mutation in myelofibrosis with myeloid metaplasia.
Pikman Y, Lee BH, Mercher T, McDowell E, Ebert BL, Gozo M, Cuker A, Wernig G, Moore S, Galinsky I, DeAngelo DJ, Clark JJ, Lee SJ, Golub TR, Wadleigh M, Gilliland DG, Levine RL.
PLoS Med. 2006 Jul;3(7):e270.
PMID 16834459
 
High occurrence of JAK2 V617 mutation in refractory anemia with ringed sideroblasts associated with marked thrombocytosis.
Renneville A, Quesnel B, Charpentier A, Terriou L, Crinquette A, Lai JL, Cossement C, Lionne-Huyghe P, Rose C, Bauters F, Preudhomme C.
Leukemia. 2006 Nov;20(11):2067-70.
PMID 16990759
 
Report on two novel nucleotide exchanges in the JAK2 pseudokinase domain: D620E and E627E.
Schnittger S, Bacher U, Kern W, Schroder M, Haferlach T, Schoch C.
Leukemia. 2006 Dec;20(12):2195-7.
PMID 16871281
 
Progenitors homozygous for the V617F mutation occur in most patients with polycythemia vera, but not essential thrombocythemia.
Scott LM, Scott MA, Campbell PJ, Green AR.
Blood. 2006 Oct 1;108(7):2435-7.
PMID 16772604
 
JAK2 V617F is a rare finding in de novo acute myeloid leukemia, but STAT3 activation is common and remains unexplained.
Steensma DP, McClure RF, Karp JE, Tefferi A, Lasho TL, Powell HL, DeWald GW, Kaufmann SH.
Leukemia. 2006 Jun;20(6):971-8.
PMID 16598306
 
Classification of chronic myeloid disorders: from Dameshek towards a semi-molecular system.
Tefferi A, Gilliland G.
Best Pract Res Clin Haematol. 2006;19(3):365-85.
PMID 16781478
 
JAK2V617F mutational frequency in polycythemia vera: 100%, >90%, less?
Verstovsek S, Silver RT, Cross NC, Tefferi A.
Leukemia. 2006 Nov;20(11):2067.
PMID 16990780
 
Expression of Jak2V617F causes a polycythemia vera-like disease with associated myelofibrosis in a murine bone marrow transplant model.
Wernig G, Mercher T, Okabe R, Levine RL, Lee BH, Gilliland DG.
Blood. 2006 Jun 1;107(11):4274-81.
PMID 16478879
 
JAK2 V617F mutational status predicts progression to large splenomegaly and leukemic transformation in primary myelofibrosis.
Barosi G, Bergamaschi G, Marchetti M, Vannucchi AM, Guglielmelli P, Antonioli E, Massa M, Rosti V, Campanelli R, Villani L, Viarengo G, Gattoni E, Gerli G, Specchia G, Tinelli C, Rambaldi A, Barbui T; Gruppo Italiano Malattie Ematologiche Maligne dell'Adulto (GIMEMA) Italian Registry of Myelofibrosis.
Blood. 2007 Dec 1;110(12):4030-6.
PMID 17712047
 
Jak2: normal function and role in hematopoietic disorders.
Ihle JN, Gilliland DG.
Curr Opin Genet Dev. 2007 Feb;17(1):8-14.
PMID 17208428
 
Clinical correlates of JAK2V617F allele burden in essential thrombocythemia.
Kittur J, Knudson RA, Lasho TL, Finke CM, Gangat N, Wolanskyj AP, Li CY, Wu W, Ketterling RP, Pardanani A, Tefferi A.
Cancer. 2007 Jun 1;109(11):2279-84.
PMID 17440984
 
SnapShot: JAK-STAT signaling.
Mertens C, Darnell JE Jr.
Cell. 2007 Nov 2;131(3):612.
PMID 17981126
 
TG101209, a small molecule JAK2-selective kinase inhibitor potently inhibits myeloproliferative disorder-associated JAK2V617F and MPLW515L/K mutations.
Pardanani A, Hood J, Lasho T, Levine RL, Martin MB, Noronha G, Finke C, Mak CC, Mesa R, Zhu H, Soll R, Gilliland DG, Tefferi A.
Leukemia. 2007 Aug;21(8):1658-68.
PMID 17541402
 
The frequency of JAK2 exon 12 mutations in idiopathic erythrocytosis patients with low serum erythropoietin levels.
Percy MJ, Scott LM, Erber WN, Harrison CN, Reilly JT, Jones FG, Green AR, McMullin MF.
Haematologica. 2007 Dec;92(12):1607-14.
PMID 18055983
 
JAK-STAT signaling: from interferons to cytokines.
Schindler C, Levy DE, Decker T.
J Biol Chem. 2007 Jul 13;282(28):20059-63.
PMID 17502367
 
Prevalance of JAK2 V617F and exon 12 mutations in polycythaemia vera.
Scott LM, Beer PA, Bench AJ, Erber WN, Green AR.
Br J Haematol. 2007a Nov;139(3):511-2.
PMID 17910642
 
JAK2 exon 12 mutations in polycythemia vera and idiopathic erythrocytosis.
Scott LM, Tong W, Levine RL, Scott MA, Beer PA, Stratton MR, Futreal PA, Erber WN, McMullin MF, Harrison CN, Warren AJ, Gilliland DG, Lodish HF, Green AR.
N Engl J Med. 2007b Feb 1;356(5):459-68.
PMID 17267906
 
Bone marrow JAK2V617F allele burden and clinical correlates in polycythemia vera.
Tefferi A, Strand JJ, Lasho TL, Knudson RA, Finke CM, Gangat N, Pardanani A, Hanson CA, Ketterling RP.
Leukemia. 2007 Sep;21(9):2074-5.
PMID 17476276
 
Leukemic blasts in transformed JAK2-V617F-positive myeloproliferative disorders are frequently negative for the JAK2-V617F mutation.
Theocharides A, Boissinot M, Girodon F, Garand R, Teo SS, Lippert E, Talmant P, Tichelli A, Hermouet S, Skoda RC.
Blood. 2007 Jul 1;110(1):375-9.
PMID 17363731
 
Prospective identification of high-risk polycythemia vera patients based on JAK2(V617F) allele burden.
Vannucchi AM, Antonioli E, Guglielmelli P, Longo G, Pancrazzi A, Ponziani V, Bogani C, Ferrini PR, Rambaldi A, Guerini V, Bosi A, Barbui T; MPD Research Consortium.
Leukemia. 2007a Sep;21(9):1952-9.
PMID 17625606
 
Clinical profile of homozygous JAK2 617V>F mutation in patients with polycythemia vera or essential thrombocythemia.
Vannucchi AM, Antonioli E, Guglielmelli P, Rambaldi A, Barosi G, Marchioli R, Marfisi RM, Finazzi G, Guerini V, Fabris F, Randi ML, De Stefano V, Caberlon S, Tafuri A, Ruggeri M, Specchia G, Liso V, Rossi E, Pogliani E, Gugliotta L, Bosi A, Barbui T.
Blood. 2007b Aug 1;110(3):840-6.
PMID 17379742
 
INCB018424, an oral, selective JAK2 inhibitor, shows significant clinical activity in a phase I/II study in patients with primary myelofibrosis (PMF) and post polycythemia vera/essential thrombocythemia myelofibrosis (Post-PV/ET MF).
Verstovsek S, Kantarjian H. Pardanani A.
Blood. 2007a;110(11):558.
 
A phase I study of XL019, a selective JAK2 inhibitor, in patients with primary myelofibrosis and post-polycythemia vera/essential thrombocythemia myelofibrosis.
Verstovsek S, Pardanani A.D, Shah NP
Blood. 2007b;110(11):553.
 
JAK2 V617F due to a novel TG --> CT mutation at nucleotides 1848-1849: diagnostic implication.
Wong CL, Ma ES, Wang CL, Lam HY, Ma SY.
Leukemia. 2007 Jun;21(6):1344-6.
PMID 17344912
 
The investigation of JAK2 mutation in Chinese myeloproliferative diseases-identification of a novel C616Y point mutation in a PV patient.
Zhang SJ, Li JY, Li WD, Song JH, Xu W, Qiu HX.
Int J Lab Hematol. 2007 Feb;29(1):71-2.
PMID 17224012
 
Two novel JAK2 exon 12 mutations in JAK2V617F-negative polycythaemia vera patients.
Butcher CM, Hahn U, To LB, Gecz J, Wilkins EJ, Scott HS, Bardy PG, D'Andrea RJ.
Leukemia. 2008 Apr;22(4):870-3.
PMID 17914411
 
TG101348, a JAK2-selective antagonist, inhibits primary hematopoietic cells derived from myeloproliferative disorder patients with JAK2V617F, MPLW515K or JAK2 exon 12 mutations as well as mutation negative patients.
Lasho TL, Tefferi A, Hood JD, Verstovsek S, Gilliland DG, Pardanani A.
Leukemia. 2008 Mar 20
PMID 18354492
 
JAK2 inhibitor therapy in myeloproliferative disorders: rationale, preclinical studies and ongoing clinical trials.
Pardanani A.
Leukemia. 2008 Jan;22(1):23-30. (review)
PMID 17882282
 
Somatic mutations of JAK2 exon 12 in patients with JAK2 (V617F)-negative myeloproliferative disorders.
Pietra D, Li S, Brisci A, Passamonti F, Rumi E, Theocharides A, Ferrari M, Gisslinger H, Kralovics R, Cremonesi L, Skoda R, Cazzola M.
Blood. 2008 Feb 1;111(3):1686-9.
PMID 17984312
 
Low JAK2V617F allele burden in primary myelofibrosis, compared to either a higher allele burden or unmutated status, is associated with inferior overall and leukemia-free survival.
Tefferi A, Lasho TL, Huang J, Finke C, Mesa RA, Li CY, Wu W, Hanson CA, Pardanani A.
Leukemia. 2008 Apr;22(4):756-61.
PMID 18216871
 
Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice.
Tiedt R, Hao-Shen H, Sobas MA, Looser R, Dirnhofer S, Schwaller J, Skoda RC.
Blood. 2008 Apr 15;111(8):3931-40.
PMID 18160670
 
Written2008-09Naseema Gangat, Ayalew Tefferi
of Hematology, Mayo Clinic, Rochester, MN, United States

Citation

This paper should be referenced as such :
Gangat, N ; Tefferi, A
JAK2 mutations in myeloproliferative neoplasms
Atlas Genet Cytogenet Oncol Haematol. 2009;13(8):612-617.
Free journal version : [ pdf ]   [ DOI ]
On line version : http://AtlasGeneticsOncology.org/Deep/JAK2mutationID20065.htm

© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Wed Nov 28 17:09:25 CET 2018

Home   Genes    Leukemias    Solid Tumors    Cancer-Prone    Deep Insight    Case Reports    Journals   Portal    Teaching   
X Y 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 NA

For comments and suggestions or contributions, please contact us

jlhuret@AtlasGeneticsOncology.org.