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BRWD3 (bromodomain and WD repeat domain containing 3)

Written2006-09Claudia Kalla
DKFZ, Div. Molecular Genetics (B060), Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany

(Note : for Links provided by Atlas : click)


HGNC Alias symbFLJ38568
LocusID (NCBI) 254065
Atlas_Id 42978
Location Xq21.1  [Link to chromosome band Xq21]
Location_base_pair Starts at 80669503 and ends at 80809877 bp from pter ( according to GRCh38/hg38-Dec_2013)  [Mapping BRWD3.png]
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)
ARHGAP20 (11q23.1)::BRWD3 (Xq21.1)BRWD3 (Xq21.1)::ARHGAP20 (11q23.1)BRWD3 (Xq21.1)::HMGN5 (Xq21.1)


  Genomic organization (A) and transcript variants (B) of BRWD3. (A) Gene structure (drawn to scale): black boxes represent exons. (B) Transcripts (drawn to scale): boxes, exons; UTR, untranslated region; light shaded box, coding region; shaded and dark shaded boxes, nucleotide sequences coding for protein domains (WD40: WD40 tandem repeats; BROMO: bromodomain). For transcript variants BRWD3-C to BRWD3-P only the largest possible coding regions are indicated, though translation of different short proteins by using the start sites of BRWD3-A and BRWD3-B might also be possible.
Description 44 exons spanning 132.7 kb genomic DNA
Transcription 5.6-6.2 kb mRNA, coding sequence: 4.2-5.4 kb
Alternative splicing results in the expression of at least 15 transcript variants (BRWD3-A to BRWD3-P): The two most abundant transcript variants A and B are the result of alternative splicing of the first four exons (BRWD3-A contains exons 1 to 4, whereas BRWD3-B starts with an extended version of exon 4). BRWD3-C to BRWD3-P represent alternatively spliced variants of A and B, which contain additional exons 6a, 6b, and/or 12a and lack exons 3, 5, 6, 7, 8, and/or 9.
Pseudogene None.


  Schematic representation of protein variants BRWD3-A and BRWD3-B as deduced from the transcripts. WD40: WD40 tandem repeats; BROMO: bromodomain. Translation initiation in exons 11 and 13 of transcripts BRWD3-C to BRWD3-P produces proteins, which retain four and three of the eight WD40 repeats, respectively.
Description The amino-terminal region consists of eight tandem WD40 repeats, which had been identified as the structural determinant of the beta -subunit of the G-proteins that mediateting transmembrane signal transduction. The carboxy terminus is predicted to contain two bromodomains with the potential to mediate protein-protein interactions in DNA-binding proteins. BRWD3-C to BRWD3-P are amino-terminally truncated versions of BRWD3-A and BRWD3-B, which retain three or four of the eight WD40 repeats and both bromodomains.
Expression Expressed in a variety of adult tissues (lymphocytes, brain, heart, kidney, placenta) and in fetal liver.
Function By performing a systematic genomewide survey for genes required for JAK/STAT pathway activity (involved in cell proliferation and haematopoiesis), the Drosophila homologon of BRWD3 was isolated as a member of the JAK/STAT signalling cascade acting downstream of JAK. In vivo analysis demonstrated that disrupted Drosophila BRWD3 functions as a suppressor of leukemia-like blood cell tumors.
Homology Drosophila melanogaster: BRWD3
Mouse: Brwd3
Pan troglodytes: BRWD3

Implicated in

Note In the tumour cells of one case of B-cell chronic lymphocytic leukemia (B-CLL), BRWD3 was affected by a translocation that rearranged the gene with ARHGAP20 (11q23). No fusion transcripts were generated. BRWD3 transcript expression is downregulated in B-CLL lymphocytes vs. CD19+ control B cells.
Disease B-cell chronic lymphocytic leukemia
Cytogenetics t(X;11)(q21;q23)
Hybrid/Mutated Gene ARHGAP20 - BRWD3
Abnormal Protein None detected.


Translocation t(X;11)(q13;q23) in B-cell chronic lymphocytic leukemia disrupts two novel genes.
Kalla C, Nentwich H, Schlotter M, Mertens D, Wildenberger K, Döhner H, Stilgenbauer S, Lichter P
Genes, chromosomes & cancer. 2005 ; 42 (2) : 128-143.
PMID 15543602
Identification of JAK/STAT signalling components by genome-wide RNA interference.
Müller P, Kuttenkeuler D, Gesellchen V, Zeidler MP, Boutros M
Nature. 2005 ; 436 (7052) : 871-875.
PMID 16094372


This paper should be referenced as such :
Kalla, C
BRWD3 (bromodomain, WD repeat domain containing 3)
Atlas Genet Cytogenet Oncol Haematol. 2007;11(1):10-11.
Free journal version : [ pdf ]   [ DOI ]

Other Leukemias implicated (Data extracted from papers in the Atlas) [ 2 ]
  t(X;11)(q13;q23) KMT2A::FOXO4
t(X;11)(q21;q23) BRWD3::ARHGAP20

External links


HGNC (Hugo)BRWD3   17342
Entrez_Gene (NCBI)BRWD3    bromodomain and WD repeat domain containing 3
AliasesBRODL; MRX93
GeneCards (Weizmann)BRWD3
Ensembl hg19 (Hinxton)ENSG00000165288 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000165288 [Gene_View]  ENSG00000165288 [Sequence]  chrX:80669503-80809877 [Contig_View]  BRWD3 [Vega]
ICGC DataPortalENSG00000165288
TCGA cBioPortalBRWD3
Genatlas (Paris)BRWD3
SOURCE (Princeton)BRWD3
Genetics Home Reference (NIH)BRWD3
Genomic and cartography
GoldenPath hg38 (UCSC)BRWD3  -     chrX:80669503-80809877 -  Xq21.1   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)BRWD3  -     Xq21.1   [Description]    (hg19-Feb_2009)
GoldenPathBRWD3 - Xq21.1 [CytoView hg19]  BRWD3 - Xq21.1 [CytoView hg38]
Genome Data Viewer NCBIBRWD3 [Mapview hg19]  
OMIM300553   300659   
Gene and transcription
Genbank (Entrez)AA716141 AK090573 AK095887 AY497046 AY497047
RefSeq transcript (Entrez)NM_153252
Consensus coding sequences : CCDS (NCBI)BRWD3
Gene ExpressionBRWD3 [ NCBI-GEO ]   BRWD3 [ EBI - ARRAY_EXPRESS ]   BRWD3 [ SEEK ]   BRWD3 [ MEM ]
Gene Expression Viewer (FireBrowse)BRWD3 [ Firebrowse - Broad ]
GenevisibleExpression of BRWD3 in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)254065
GTEX Portal (Tissue expression)BRWD3
Human Protein AtlasENSG00000165288-BRWD3 [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ6RI45   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtQ6RI45  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProQ6RI45
Domaine pattern : Prosite (Expaxy)BROMODOMAIN_2 (PS50014)    WD_REPEATS_1 (PS00678)    WD_REPEATS_2 (PS50082)    WD_REPEATS_REGION (PS50294)   
Domains : Interpro (EBI)Bromodomain    Bromodomain-like_sf    WD40/YVTN_repeat-like_dom_sf    WD40_repeat    WD40_repeat_CS    WD40_repeat_dom    WD40_repeat_dom_sf   
Domain families : Pfam (Sanger)Bromodomain (PF00439)    WD40 (PF00400)   
Domain families : Pfam (NCBI)pfam00439    pfam00400   
Domain families : Smart (EMBL)BROMO (SM00297)  WD40 (SM00320)  
Conserved Domain (NCBI)BRWD3
AlphaFold pdb e-kbQ6RI45   
Human Protein Atlas [tissue]ENSG00000165288-BRWD3 [tissue]
Protein Interaction databases
IntAct (EBI)Q6RI45
Ontologies - Pathways
Ontology : AmiGOnucleus  regulation of transcription by RNA polymerase II  cytoskeleton organization  cytoskeleton organization  regulation of cell shape  regulation of cell shape  
Ontology : EGO-EBInucleus  regulation of transcription by RNA polymerase II  cytoskeleton organization  cytoskeleton organization  regulation of cell shape  regulation of cell shape  
NDEx NetworkBRWD3
Atlas of Cancer Signalling NetworkBRWD3
Wikipedia pathwaysBRWD3
Orthology - Evolution
GeneTree (enSembl)ENSG00000165288
Phylogenetic Trees/Animal Genes : TreeFamBRWD3
Homologs : HomoloGeneBRWD3
Homology/Alignments : Family Browser (UCSC)BRWD3
Gene fusions - Rearrangements
Fusion : QuiverBRWD3
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerBRWD3 [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)BRWD3
Exome Variant ServerBRWD3
GNOMAD BrowserENSG00000165288
Varsome BrowserBRWD3
ACMGBRWD3 variants
Genomic Variants (DGV)BRWD3 [DGVbeta]
DECIPHERBRWD3 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisBRWD3 
ICGC Data PortalBRWD3 
TCGA Data PortalBRWD3 
Broad Tumor PortalBRWD3
OASIS PortalBRWD3 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICBRWD3  [overview]  [genome browser]  [tissue]  [distribution]  
Somatic Mutations in Cancer : COSMIC3DBRWD3
Mutations and Diseases : HGMDBRWD3
LOVD (Leiden Open Variation Database)[gene] [transcripts] [variants]
DgiDB (Drug Gene Interaction Database)BRWD3
DoCM (Curated mutations)BRWD3
CIViC (Clinical Interpretations of Variants in Cancer)BRWD3
NCG (London)BRWD3
Impact of mutations[PolyPhen2] [Provean] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
OMIM300553    300659   
Genetic Testing Registry BRWD3
NextProtQ6RI45 [Medical]
Target ValidationBRWD3
Huge Navigator BRWD3 [HugePedia]
ClinGenBRWD3 (curated)
Clinical trials, drugs, therapy
Protein Interactions : CTDBRWD3
Pharm GKB GenePA134900775
Clinical trialBRWD3
DataMed IndexBRWD3
PubMed24 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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indexed on : Fri Oct 8 21:13:51 CEST 2021

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