Description | 982 amino acids; 120 kDa; the overall structure of CBLb is very close to the sructure of CBL: the evolutionarily conserved aminoterminal region of CBL is composed of three interacting domains: a four-helix bundle (4H), an EF-hand calcium binding domain, and a divergent SH2 domain; the three domains together form an integrated phosphoprotein -recognition module; this aminoterminal region is followed by a central Ring finger with a Cys3HisCys4 motif and an aminoterminal region with multiple proline-rich sequences, a putative leucine zipper and several potential tyrosine phosphorylation sites; two alternatively spliced mRNA code form CBLb proteins lacking the leucine zipper |
Expression | expression of CBLb was seen in several human adult tissues including lung, spleen and testis but not in human brain, liver, pancreas, salivary gland or skeletal muscle; expression was also detected in fetal brain and liver, in hematopoietic cell lines and in normal and malignant mammary epithelial cell lines |
Localisation | cytoplasmic |
Function | regulation of signal transduction; CBLb has been shown to inhibit c-Jun terminal kinase (JNK) activation mediated by the (VAV oncoprotein and EGF receptor (EGFR) signaling; in contrast, a direct interaction between CBLb and the kinase ZAP-70 induces a positive signal in T cells |
Cbl-b, a member of the Sli-1/c-Cbl protein family, inhibits Vav-mediated c-Jun N-terminal kinase activation. |
Bustelo XR, Crespo P, López-Barahona M, Gutkind JS, Barbacid M |
Oncogene. 1997 ; 15 (21) : 2511-2520. |
PMID 9399639 |
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cbl-b inhibits epidermal growth factor receptor signaling. |
Ettenberg SA, Keane MM, Nau MM, Frankel M, Wang LM, Pierce JH, Lipkowitz S |
Oncogene. 1999 ; 18 (10) : 1855-1866. |
PMID 10086340 |
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Cloning and characterization of cbl-b: a SH3 binding protein with homology to the c-cbl proto-oncogene. |
Keane MM, Rivero-Lezcano OM, Mitchell JA, Robbins KC, Lipkowitz S |
Oncogene. 1995 ; 10 (12) : 2367-2377. |
PMID 7784085 |
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The CBL-related protein CBLB participates in FLT3 and interleukin-7 receptor signal transduction in pro-B cells. |
Lavagna-Sé C, Marchetto S, Birnbaum D, Rosnet O |
The Journal of biological chemistry. 1998 ; 273 (24) : 14962-14967. |
PMID 9614102 |
|
A direct interaction between the adaptor protein Cbl-b and the kinase zap-70 induces a positive signal in T cells. |
Zhang Z, Elly C, Qiu L, Altman A, Liu YC |
Current biology : CB. 1999 ; 9 (4) : 203-206. |
PMID 10074432 |
|