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EIF3F (eukaryotic translation initiation factor 3, subunit F)

Identity

Other namesEIF3S5
eIF-3-epsilon
eIF3-epsilon
eIF3-p47
eIF3f
eIF3 subunit p47
p47
HGNC (Hugo) EIF3F
LocusID (NCBI) 8665
Location 11p15.4
Location_base_pair Starts at 8008867 and ends at 8017719 bp from pter ( according to hg19-Feb_2009)  [Mapping]
Note eIF3f is one of at least 13 subunits comprising the eukaryotic initiation factor 3 (eIF3) complex.

DNA/RNA

Description 10.82kb long.
Transcription 8 exons; transcript length: 3,228 bps; 1073 bp coding sequence.
Pseudogene Chromosome 2 from 58,332,079 to 58,333,164 bp (AC007250.3, Ensembl).

Protein

Description 357 amino acids; 37,563.75 Da; Isoelectric point: 5.122; Mov34/MPN/PAD1 domain.
Expression Ubiquitous.
Localisation Cytoplasmic. There is also evidence of its existence in the nucleus.
Function eIF3f is a subunit of eIF3 complex and is highly conserved among species. eIF3f interacts with the caspase-processed isoform of CDK11 (CDK11p46), which appears to be a down-stream effector in apoptotic signaling. eIF3f can be phosphorylated at Ser46 and Thr119 by CDK11p46 during apoptosis. The phosphorylation of eIF3f contributes to translation inhibition and apoptosis. The expression of eIF3f is significantly decreased in pancreatic cancer and melanoma. Loss of the eIF3f allele has been reported in pancreatic cancer and melanoma. Ectopic expression of eIF3f causes rRNA degradation, inhibits translation and cell proliferation, and induces apoptosis in pancreatic cancer and melanoma cells. On the other hand, knockdown of eIF3f prevents apoptosis in pancreatic cancer and melanoma cells. Recent studies also showed decreased cell growth, cell proliferation, colony formation and increased apoptosis in eIF3f-overexpressing NIH3T3 cells.
Homology eIF3f is not found in S. cerevisiae; However it is found in Schizosaccharomyces pombe (SPBC4C3.07, 35% identity) and in Drosophila Melanogaster (CG9769, 49% identity).

Mutations

Note No eIF3f mutations have been reported.

Implicated in

Entity Pancreatic cancer
Disease Decreased eIF3f mRNA and protein was found in pancreatic cancer.
Oncogenesis Decreased eIF3f gene expression may result from loss of eIF3f gene allele and down regulation of transcription. Overexpression of eIF3f in pancreatic cancer and melanoma cells can lead to 28S rRNA degradation, decreased translation and increased apoptosis. On the other hand, knockdown of eIF3f attenuates apoptosis in tumor cells.
  
Entity Melanoma
Disease Decreased eIF3f mRNA and protein was found in melanoma.
Oncogenesis See above
  
Entity Muscle atrophy
Disease eIF3f is a key target of MAFbx, an E3 ubiquitin ligase, during muscle atrophy and has a major role in skeletal muscle hypertrophy. During muscle atrophy, MAFbx targets eIF3f for ubiquitination and degradation. Thus, eIF3f appears to be an attractive therapeutic target.
  

To be noted

eIF3f is a potential tumor suppressor in pancreatic cancer and melanoma. Loss of heterozygosity and decreased expression of eIF3f has been found in most human pancreatic cancer and melanoma specimens. Silencing of eIF3f increases cell proliferation and colony formation. Restoration of eIF3f expression induces cancer cell death.

External links

Nomenclature
HGNC (Hugo)EIF3F   3275
Cards
AtlasEIF3FID44407ch11p15
Entrez_Gene (NCBI)EIF3F  8665  eukaryotic translation initiation factor 3, subunit F
GeneCards (Weizmann)EIF3F
Ensembl (Hinxton)ENSG00000175390 [Gene_View]  chr11:8008867-8017719 [Contig_View]  EIF3F [Vega]
AceView (NCBI)EIF3F
Genatlas (Paris)EIF3F
WikiGenes8665
SOURCE (Princeton)NM_003754
Genomic and cartography
GoldenPath (UCSC)EIF3F  -  11p15.4   chr11:8008867-8017719 +  11p15.4   [Description]    (hg19-Feb_2009)
EnsemblEIF3F - 11p15.4 [CytoView]
Mapping of homologs : NCBIEIF3F [Mapview]
OMIM603914   
Gene and transcription
Genbank (Entrez)AK027739 AK093511 AK095574 AK289637 AK291354
RefSeq transcript (Entrez)NM_003754
RefSeq genomic (Entrez)AC_000143 NC_000011 NC_018922 NT_009237 NW_001838021 NW_004929378
Consensus coding sequences : CCDS (NCBI)EIF3F
Cluster EST : UnigeneHs.516023 [ NCBI ]
CGAP (NCI)Hs.516023
Alternative Splicing : Fast-db (Paris)GSHG0004618
Alternative Splicing GalleryENSG00000175390
Gene ExpressionEIF3F [ NCBI-GEO ]     EIF3F [ SEEK ]   EIF3F [ MEM ]
Protein : pattern, domain, 3D structure
UniProt/SwissProtO00303 (Uniprot)
NextProtO00303  [Medical]
With graphics : InterProO00303
Splice isoforms : SwissVarO00303 (Swissvar)
Catalytic activity : Enzyme3.4.19.12 [ Enzyme-Expasy ]   3.4.19.123.4.19.12 [ IntEnz-EBI ]   3.4.19.12 [ BRENDA ]   3.4.19.12 [ KEGG ]   
Domains : Interpro (EBI)eIF3f    JAB_MPN_dom    Rpn11/EIF3F_C   
Related proteins : CluSTrO00303
Domain families : Pfam (Sanger)JAB (PF01398)    MitMem_reg (PF13012)   
Domain families : Pfam (NCBI)pfam01398    pfam13012   
Domain families : Smart (EMBL)JAB_MPN (SM00232)  
DMDM Disease mutations8665
Blocks (Seattle)O00303
Human Protein AtlasENSG00000175390
Peptide AtlasO00303
HPRD04887
IPIIPI00941255   IPI00654777   IPI01013021   IPI01013988   IPI00979872   
Protein Interaction databases
DIP (DOE-UCLA)O00303
IntAct (EBI)O00303
FunCoupENSG00000175390
BioGRIDEIF3F
InParanoidO00303
Interologous Interaction database O00303
IntegromeDBEIF3F
STRING (EMBL)EIF3F
Ontologies - Pathways
Ontology : AmiGOformation of translation preinitiation complex  translation initiation factor activity  translation initiation factor activity  translation initiation factor activity  protein binding  cytosol  eukaryotic translation initiation factor 3 complex  eukaryotic translation initiation factor 3 complex  translation  translational initiation  translational initiation  translational initiation  regulation of translational initiation  proteolysis  cysteine-type peptidase activity  gene expression  eukaryotic 43S preinitiation complex  eukaryotic 48S preinitiation complex  cellular protein metabolic process  
Ontology : EGO-EBIformation of translation preinitiation complex  translation initiation factor activity  translation initiation factor activity  translation initiation factor activity  protein binding  cytosol  eukaryotic translation initiation factor 3 complex  eukaryotic translation initiation factor 3 complex  translation  translational initiation  translational initiation  translational initiation  regulation of translational initiation  proteolysis  cysteine-type peptidase activity  gene expression  eukaryotic 43S preinitiation complex  eukaryotic 48S preinitiation complex  cellular protein metabolic process  
Pathways : KEGGRNA transport   
REACTOMEEIF3F
Protein Interaction DatabaseEIF3F
Wikipedia pathwaysEIF3F
Gene fusion - rearrangments
Polymorphisms : SNP, mutations, diseases
SNP Single Nucleotide Polymorphism (NCBI)EIF3F
SNP (GeneSNP Utah)EIF3F
SNP : HGBaseEIF3F
Genetic variants : HAPMAPEIF3F
1000_GenomesEIF3F 
ICGC programENSG00000175390 
Somatic Mutations in Cancer : COSMICEIF3F 
CONAN: Copy Number AnalysisEIF3F 
Mutations and Diseases : HGMDEIF3F
OMIM603914   
GENETestsEIF3F
Disease Genetic AssociationEIF3F
Huge Navigator EIF3F [HugePedia]  EIF3F [HugeCancerGEM]
Genomic VariantsEIF3F  EIF3F [DGVbeta]
Exome VariantEIF3F
dbVarEIF3F
ClinVarEIF3F
snp3D : Map Gene to Disease8665
General knowledge
Homologs : HomoloGeneEIF3F
Homology/Alignments : Family Browser (UCSC)EIF3F
Phylogenetic Trees/Animal Genes : TreeFamEIF3F
Chemical/Protein Interactions : CTD8665
Chemical/Pharm GKB GenePA162384806
Clinical trialEIF3F
Cancer Resource (Charite)ENSG00000175390
Other databases
Probes
Litterature
PubMed55 Pubmed reference(s) in Entrez
CoreMineEIF3F
iHOPEIF3F

Bibliography

The p34cdc2-related cyclin-dependent kinase 11 interacts with the p47 subunit of eukaryotic initiation factor 3 during apoptosis.
Shi J, Feng Y, Goulet AC, Vaillancourt RR, Sachs NA, Hershey JW, Nelson MA.
J Biol Chem. 2003 Feb 14;278(7):5062-71. Epub 2002 Nov 20.
PMID 12446680
 
Decreased expression of eukaryotic initiation factor 3f deregulates translation and apoptosis in tumor cells.
Shi J, Kahle A, Hershey JW, Honchak BM, Warneke JA, Leong SP, Nelson MA.
Oncogene. 2006 Aug 10;25(35):4923-36. Epub 2006 Mar 13.
PMID 16532022
 
Reconstitution reveals the functional core of mammalian eIF3.
Masutani M, Sonenberg N, Yokoyama S, Imataka H.
EMBO J. 2007 Jul 25;26(14):3373-83. Epub 2007 Jun 21.
PMID 17581632
 
Loss of the eukaryotic initiation factor 3f in pancreatic cancer.
Doldan A, Chandramouli A, Shanas R, Bhattacharyya A, Cunningham JT, Nelson MA, Shi J.
Mol Carcinog. 2008 Mar;47(3):235-44.
PMID 17918192
 
Loss of the eukaryotic initiation factor 3f in melanoma.
Doldan A, Chandramouli A, Shanas R, Bhattacharyya A, Leong SP, Nelson MA, Shi J.
Mol Carcinog. 2008 Oct;47(10):806-13.
PMID 18381585
 
All translation elongation factors and the e, f, and h subunits of translation initiation factor 3 are encoded by 5'-terminal oligopyrimidine (TOP) mRNAs.
Iadevaia V, Caldarola S, Tino E, Amaldi F, Loreni F.
RNA. 2008 Sep;14(9):1730-6. Epub 2008 Jul 24.
PMID 18658124
 
The initiation factor eIF3-f is a major target for atrogin1/MAFbx function in skeletal muscle atrophy.
Lagirand-Cantaloube J, Offner N, Csibi A, Leibovitch MP, Batonnet-Pichon S, Tintignac LA, Segura CT, Leibovitch SA.
EMBO J. 2008 Apr 23;27(8):1266-76. Epub 2008 Mar 20.
PMID 18354498
 
mRNA retrotransposition coupled with 5' inversion as a possible source of new genes.
Kojima KK, Okada N.
Mol Biol Evol. 2009 Jun;26(6):1405-20. Epub 2009 Mar 16.
PMID 19289598
 
Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis.
Shi J, Hershey JW, Nelson MA.
FEBS Lett. 2009 Mar 18;583(6):971-7. Epub 2009 Feb 24.
PMID 19245811
 
Inhibition of HIV-1 replication by eIF3f.
Valente ST, Gilmartin GM, Mott C, Falkard B, Goff SP.
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4071-8. Epub 2009 Feb 23.
PMID 19237569
 
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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Contributor(s)

Written07-2009Jiaqi Shi, Mark A Nelson
Department of Surgery, Arizona Cancer Center, University of Arizona, USA (JS); Department of Pathology, Arizona Cancer Center, University of Arizona, USA (JS, MN)

Citation

This paper should be referenced as such :
Shi J, Nelson MA . EIF3F (eukaryotic translation initiation factor 3, subunit F). Atlas Genet Cytogenet Oncol Haematol. July 2009 .
URL : http://AtlasGeneticsOncology.org/Genes/EIF3FID44407ch11p15.html

The various updated versions of this paper are referenced and archived by INIST as such :
http://documents.irevues.inist.fr/bitstream/2042/44774/1/07-2009-EIF3FID44407ch11p15.pdf   [ Bibliographic record ]

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indexed on : Fri Apr 18 17:34:30 CEST 2014

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