| Identity |
| Other names | EIF3S5 |
| eIF-3-epsilon | |
| eIF3-epsilon | |
| eIF3-p47 | |
| eIF3f | |
| eIF3 subunit p47 | |
| p47 | |
| HGNC (Hugo) | EIF3F |
| LocusID (NCBI) | 8665 |
| Location | 11p15.4 |
| Location_base_pair | Starts at 8008867 and ends at 8017719 bp from pter ( according to hg19-Feb_2009) [Mapping] |
| Note | eIF3f is one of at least 13 subunits comprising the eukaryotic initiation factor 3 (eIF3) complex. |
| DNA/RNA |
| Description | 10.82kb long. |
| Transcription | 8 exons; transcript length: 3,228 bps; 1073 bp coding sequence. |
| Pseudogene | Chromosome 2 from 58,332,079 to 58,333,164 bp (AC007250.3, Ensembl). |
| Protein |
| Description | 357 amino acids; 37,563.75 Da; Isoelectric point: 5.122; Mov34/MPN/PAD1 domain. |
| Expression | Ubiquitous. |
| Localisation | Cytoplasmic. There is also evidence of its existence in the nucleus. |
| Function | eIF3f is a subunit of eIF3 complex and is highly conserved among species. eIF3f interacts with the caspase-processed isoform of CDK11 (CDK11p46), which appears to be a down-stream effector in apoptotic signaling. eIF3f can be phosphorylated at Ser46 and Thr119 by CDK11p46 during apoptosis. The phosphorylation of eIF3f contributes to translation inhibition and apoptosis. The expression of eIF3f is significantly decreased in pancreatic cancer and melanoma. Loss of the eIF3f allele has been reported in pancreatic cancer and melanoma. Ectopic expression of eIF3f causes rRNA degradation, inhibits translation and cell proliferation, and induces apoptosis in pancreatic cancer and melanoma cells. On the other hand, knockdown of eIF3f prevents apoptosis in pancreatic cancer and melanoma cells. Recent studies also showed decreased cell growth, cell proliferation, colony formation and increased apoptosis in eIF3f-overexpressing NIH3T3 cells. |
| Homology | eIF3f is not found in S. cerevisiae; However it is found in Schizosaccharomyces pombe (SPBC4C3.07, 35% identity) and in Drosophila Melanogaster (CG9769, 49% identity). |
| Mutations |
| Note | No eIF3f mutations have been reported. |
| Implicated in |
| Entity | Pancreatic cancer |
| Disease | Decreased eIF3f mRNA and protein was found in pancreatic cancer. |
| Oncogenesis | Decreased eIF3f gene expression may result from loss of eIF3f gene allele and down regulation of transcription. Overexpression of eIF3f in pancreatic cancer and melanoma cells can lead to 28S rRNA degradation, decreased translation and increased apoptosis. On the other hand, knockdown of eIF3f attenuates apoptosis in tumor cells. |
| Entity | Melanoma |
| Disease | Decreased eIF3f mRNA and protein was found in melanoma. |
| Oncogenesis | See above |
| Entity | Muscle atrophy |
| Disease | eIF3f is a key target of MAFbx, an E3 ubiquitin ligase, during muscle atrophy and has a major role in skeletal muscle hypertrophy. During muscle atrophy, MAFbx targets eIF3f for ubiquitination and degradation. Thus, eIF3f appears to be an attractive therapeutic target. |
| To be noted |
| eIF3f is a potential tumor suppressor in pancreatic cancer and melanoma. Loss of heterozygosity and decreased expression of eIF3f has been found in most human pancreatic cancer and melanoma specimens. Silencing of eIF3f increases cell proliferation and colony formation. Restoration of eIF3f expression induces cancer cell death. |
| External links |
| Bibliography |
| The p34cdc2-related cyclin-dependent kinase 11 interacts with the p47 subunit of eukaryotic initiation factor 3 during apoptosis. |
| Shi J, Feng Y, Goulet AC, Vaillancourt RR, Sachs NA, Hershey JW, Nelson MA. |
| J Biol Chem. 2003 Feb 14;278(7):5062-71. Epub 2002 Nov 20. |
| PMID 12446680 |
| Decreased expression of eukaryotic initiation factor 3f deregulates translation and apoptosis in tumor cells. |
| Shi J, Kahle A, Hershey JW, Honchak BM, Warneke JA, Leong SP, Nelson MA. |
| Oncogene. 2006 Aug 10;25(35):4923-36. Epub 2006 Mar 13. |
| PMID 16532022 |
| Reconstitution reveals the functional core of mammalian eIF3. |
| Masutani M, Sonenberg N, Yokoyama S, Imataka H. |
| EMBO J. 2007 Jul 25;26(14):3373-83. Epub 2007 Jun 21. |
| PMID 17581632 |
| Loss of the eukaryotic initiation factor 3f in pancreatic cancer. |
| Doldan A, Chandramouli A, Shanas R, Bhattacharyya A, Cunningham JT, Nelson MA, Shi J. |
| Mol Carcinog. 2008 Mar;47(3):235-44. |
| PMID 17918192 |
| Loss of the eukaryotic initiation factor 3f in melanoma. |
| Doldan A, Chandramouli A, Shanas R, Bhattacharyya A, Leong SP, Nelson MA, Shi J. |
| Mol Carcinog. 2008 Oct;47(10):806-13. |
| PMID 18381585 |
| All translation elongation factors and the e, f, and h subunits of translation initiation factor 3 are encoded by 5'-terminal oligopyrimidine (TOP) mRNAs. |
| Iadevaia V, Caldarola S, Tino E, Amaldi F, Loreni F. |
| RNA. 2008 Sep;14(9):1730-6. Epub 2008 Jul 24. |
| PMID 18658124 |
| The initiation factor eIF3-f is a major target for atrogin1/MAFbx function in skeletal muscle atrophy. |
| Lagirand-Cantaloube J, Offner N, Csibi A, Leibovitch MP, Batonnet-Pichon S, Tintignac LA, Segura CT, Leibovitch SA. |
| EMBO J. 2008 Apr 23;27(8):1266-76. Epub 2008 Mar 20. |
| PMID 18354498 |
| mRNA retrotransposition coupled with 5' inversion as a possible source of new genes. |
| Kojima KK, Okada N. |
| Mol Biol Evol. 2009 Jun;26(6):1405-20. Epub 2009 Mar 16. |
| PMID 19289598 |
| Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis. |
| Shi J, Hershey JW, Nelson MA. |
| FEBS Lett. 2009 Mar 18;583(6):971-7. Epub 2009 Feb 24. |
| PMID 19245811 |
| Inhibition of HIV-1 replication by eIF3f. |
| Valente ST, Gilmartin GM, Mott C, Falkard B, Goff SP. |
| Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4071-8. Epub 2009 Feb 23. |
| PMID 19237569 |
| REVIEW articles | automatic search in PubMed |
| Last year publications | automatic search in PubMed |
| Contributor(s) |
| Written | 07-2009 | Jiaqi Shi, Mark A Nelson |
| Department of Surgery, Arizona Cancer Center, University of Arizona, USA (JS); Department of Pathology, Arizona Cancer Center, University of Arizona, USA (JS, MN) |
| Citation |
| This paper should be referenced as such : |
| Shi J, Nelson MA . EIF3F (eukaryotic translation initiation factor 3, subunit F). Atlas Genet Cytogenet Oncol Haematol. July 2009 . URL : http://AtlasGeneticsOncology.org/Genes/EIF3FID44407ch11p15.html |
This paper is referenced by INIST as such : |
| http://documents.irevues.inist.fr/bitstream/2042/44774/1/07-2009-EIF3FID44407ch11p15.pdf [ Bibliographic record ] |
| © Atlas of Genetics and Cytogenetics in Oncology and Haematology | indexed on : Wed May 1 13:06:04 CEST 2013 |
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