Atlas of Genetics and Cytogenetics in Oncology and Haematology
EPHA7 (EPH receptor A7)
| Identity |
| Other names | EHK3 |
| HEK11 | |
| HGNC (Hugo) | EPHA7 |
| LocusID (NCBI) | 2045 |
| Location | 6q16.1 |
| Location_base_pair | Starts at 93949740 and ends at 94129300 bp from pter ( according to hg19-Feb_2009) [Mapping] |
 | |
| Probe(s) - Courtesy Mariano Rocchi, Resources for Molecular Cytogenetics | |
| DNA/RNA |
| Description | The EPHA7 gene maps on chromosome 6q16.1 spanning 178,134 bp. it contains 17 exons, the orientation of the gene is complement. |
| Transcription | rTanscript of 5,229 bp. |
| Protein |
 | |
| Description | EPHA7 encodes 998 amino acids, theoretical pI is 5.58, theoretical molecular weight is 112 KDa, tyrosine kinase, catalytic domain, sterile alpha motif, 2 fibronectin type 3 domains, ephrin receptor ligand binding domain and tumor necrosis factor receptor domain. |
| Expression | in brain, skeletal muscle, lung, kidney, liver, colorectum and nerve system. |
| Localisation | Located in the membrane. |
| Function | ATP Binding, ephrin receptor activity, nucleotide binding, protein binding, receptor activity, transferase activity. |
| Homology | Homo sapiens: EPHA5 isoform b [NP_872272] (64%), EPHA5 isoform a [NP_004430] (63%), EPHA4 [NP_004429] (63%), EPHA3 [AAG43576] (63%). |
| Implicated in |
| Entity | Colorectal cancer |
| Note | A significant reduction of EphA7 expression in human colorectal cancers was shown using semiquantitative reverse transcription-polymerase chain reaction analysis in 59 colorectal cancer tissues, compared to corresponding normal mucosas (P=0.008), and five colon cancer cell lines. To investigate the mechanism of EphA7 downregulation in colorectal cancer, we examined the methylation status of the 5'CpG island around the translation start site in five colon cancer cell lines using restriction enzymes, methylation-specific PCR, and bisulfite sequencing and found evidence of aberrant methylation. The expression of EphA7 in colon cancer cell lines was restored after treatment with 5-aza-2'-deoxycytidine. Analysis of methylation status in totally 75 tumors compared to clinicopathological parameters revealed that hypermethylation of colorectal cancers was more frequent in male than in female, and in moderately differentiated than in well-differentiated adenocarcinomas. There was a tendency that hypermethylation in rectal cancers was more frequent than in colon cancers. Hypermethylation was also observed in colorectal adenomas. This is the first report describing the downregulation of an Eph family gene in a solid tumor via aberrant 5'CpG island methylation. It provides the evidence that EphA7 gene is involved in human colorectal carcinogenesis. |
| External links |
| Bibliography |
| Regulation of repulsion versus adhesion by different splice forms of an Eph receptor. |
| Holmberg J, Clarke DL, Frisˆ©n J |
| Nature. 2000 ; 408 (6809) : 203-206. |
| PMID 11089974 |
| Differential gene expression of Eph receptors and ephrins in benign human tissues and cancers. |
| Hafner C, Schmitz G, Meyer S, Bataille F, Hau P, Langmann T, Dietmaier W, Landthaler M, Vogt T |
| Clinical chemistry. 2004 ; 50 (3) : 490-499. |
| PMID 14726470 |
| Downregulation of EphA7 by hypermethylation in colorectal cancer. |
| Wang J, Kataoka H, Suzuki M, Sato N, Nakamura R, Tao H, Maruyama K, Isogaki J, Kanaoka S, Ihara M, Tanaka M, Kanamori M, Nakamura T, Shinmura K, Sugimura H |
| Oncogene. 2005 ; 24 (36) : 5637-5647. |
| PMID 16007213 |
| Inhibition of EphA7 up-regulation after spinal cord injury reduces apoptosis and promotes locomotor recovery. |
| Figueroa JD, Benton RL, Velazquez I, Torrado AI, Ortiz CM, Hernandez CM, Diaz JJ, Magnuson DS, Whittemore SR, Miranda JD |
| Journal of neuroscience research. 2006 ; 84 (7) : 1438-1451. |
| PMID 16983667 |
| Expression profile of Eph receptors and ephrin ligands in human skin and downregulation of EphA1 in nonmelanoma skin cancer. |
| Hafner C, Becker B, Landthaler M, Vogt T |
| Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 2006 ; 19 (10) : 1369-1377. |
| PMID 16862074 |
| Absence of tyrosine kinase mutations in Japanese colorectal cancer patients. |
| Shao RX, Kato N, Lin LJ, Muroyama R, Moriyama M, Ikenoue T, Watabe H, Otsuka M, Guleng B, Ohta M, Tanaka Y, Kondo S, Dharel N, Chang JH, Yoshida H, Kawabe T, Omata M |
| Oncogene. 2007 ; 26 (14) : 2133-2135. |
| PMID 17016444 |
| Mutations in HOXD13 underlie syndactyly type V and a novel brachydactyly-syndactyly syndrome. |
| Zhao X, Sun M, Zhao J, Leyva JA, Zhu H, Yang W, Zeng X, Ao Y, Liu Q, Liu G, Lo WH, Jabs EW, Amzel LM, Shan X, Zhang X |
| American journal of human genetics. 2007 ; 80 (2) : 361-371. |
| PMID 17236141 |
| REVIEW articles | automatic search in PubMed |
| Last year publications | automatic search in PubMed |
| Contributor(s) |
| Written | 02-2007 | Haruhiko Sugimura, Hiroki Mori, Tomoyasu Bunai, Masaya Suzuki |
| Citation |
| This paper should be referenced as such : |
| Sugimura H, Mori H, Bunai T, Suzuki M . EPHA7 (EPH receptor A7). Atlas Genet Cytogenet Oncol Haematol. February 2007 . URL : http://AtlasGeneticsOncology.org/Genes/EPHA7ID40466ch6q16.html |
This paper is referenced by INIST as such : |
| http://documents.irevues.inist.fr/bitstream/2042/38435/1/02-2007-EPHA7ID40466ch6q16.pdf [ Bibliographic record ] |
| © Atlas of Genetics and Cytogenetics in Oncology and Haematology | indexed on : Wed May 1 13:04:50 CEST 2013 |
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