The gene spans approximately 30 kb and contained 14 exons. The largest exon (901 bp) contains the end of the ETS domain, the carboxy-terminal domain and the 3-untranslated region. The remaining exons varied from 48 bp (exon 5) to 266 bp (exon 9).

Nuclear (Monté et al., 1994; Takahashi et al., 2005). Ubiquitinated protein is localized in the dot-like structure in the nuclear (Takahashi et al., 2005).

ETV4 is capable of regulating transcription by binding to the Ets-binding site in the promoter of its target genes. Biologically, it contributes in a number of processes including neuronal pathfinding, mammary gland development, and male sexual function (Laing et al., 2000; Ladle et al., 2002; Kurpios et al., 2003). In various malignancies, its over-expression has been observed and it was also associated with tumor progression and outcome of patients with these malignancies. As mechanism of such function, regulation of hepatocyte growth factor (HGF)-induced cell migration (Hakuma et al., 2005), HER2-mediated malignant potential (Benz et al., 1997), and other ETV4-related factors including cyclooxygenase (COX)-2 and matrix metalloproteinases (MMPs) have been reported (Higashino et al., 1995; Horiuchi et al., 2003; Shindoh et al., 2004). In addition, ETV4 promotes cell cycle progression via upregulation of cyclin D3 transcription in breast cancer cell (MDA231 cell) (Jiang et al., 2007). In contrast, ETV4 function as negative regulator of sonic hedgehog expression (Mao et al., 2009).