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CAPG (capping protein (actin filament), gelsolin-like)

Identity

Other namesAFCP
MCP
HGNC (Hugo) CAPG
LocusID (NCBI) 822
Location 2p11.2
Location_base_pair Starts at 85621871 and ends at 85641197 bp from pter ( according to hg19-Feb_2009)  [Mapping]

DNA/RNA

 
Description DNA size: 15.58 kb; mRNA size: 1220 bp; 10 exons.

Protein

 
Description 348 amino acids; 38.518 kDa. CapG protein contains three gelsolin-like repeats in the position 27-75 (49), 148-188 (41), and 261-307 (47); and a nuclear localization signal (NES) 137-146 (10). Three natural variants, VAR-047776 (V41I), VAR-047777 (R198W), and VAR-047778 (R335H) have been reported.
Isoforms: Altogether 15 different isoforms (eight complete, one COOH complete, and six partial) have been predicted. Isoform jApr07 is annotated, contains 79 amino acids N-terminal to the first AUG.
Expression Ubiquitously expressed; highly expressed in prostate, lung, and kidney.
Localisation Cytoplasmic (52.2 %), cytoskeletal (21.7 %), and nuclear (13.0 %), according to PSORT II prediction.
Function CAPG encodes a member of the gelsolin/villin family of actin-regulatory proteins that reversibly blocks (capping) the barbed ends of F-actin filaments in a Ca 2+and phosphoinositide-regulated manner, but does not sever preformed actin filaments. Capping contributes to the control of actin-based motility in non-muscle cells. Dysregulation of actin-based motility is a prominent factor in cell transformation and is probably associated with carcinogenesis. CAPG is also involved in cell signaling, receptor-mediated membrane ruffling, and phagocytosis.
Homology The percent identity below represents identity of CAPG over an aligned region in UniGene.
- Pan troglodytes: 99.7 % (percentage identity)
- Macaca mulatta: 99.7 %
- Mus musculus: 95.4 %
- Rattus norvegicus: 92.8 %
- Bos taurus: 91.1 %
- Danio rerio: 61.7 %

Implicated in

Entity Breast cancer
Disease CAPG is expressed at higher levels in metastasizing breast cancer, than in normal breast epithelium. Furthermore, CapG protein is also transported more rapidly into the nucleus of the breast cancerous cells than the corresponding benign cells. CapG knockdown reduces invasiveness in Matrigel invasion assay, which supports the role of CAPG in cell motility. Thus, increased expression of CAPG leads to dysregulation of cell motility that promotes tumor invasion.
  
Entity Oral carcinogenesis
Note Significant overexpression of CapG protein was detected in oral squamous-cell carcinoma cells (OSCCs) and oral premalignant lesions (OPLs). Enhanced expression of CapG was also associated with large tumour size and advanced staging of OSCCs. Therefore, CapG may initiate or activate the neoplastic process of OSCC cells including the regulation of biologic behaviour of aggressive forms of cells rather than as a suppressor.
  
Entity Ovarian carcinomas
Disease In a Swedish women population, CAPG was expressed more in tumors among deceased patients than survivors with ovarian carcinomas. The stage III serous papillary adenocarcinomas of the ovary show higher CAPG expression and this is possibly correlated with the advance disease stage. Overexpression of CAPG promotes aggressive tumor progression.
  
Entity Pancreatic cancer
Note Up regulation and multiple isoforms of CapG were detected in pancreatic cancer tissue and cell lines. High level of CapG protein may possibly play an important role in pancreatic cancer cell motility and consequently dissemination.
  
Entity Ocular melanoma
Note CapG, which is a unique regulator of the actin cytoskeleton, is expressed in malignant melanomas of the uvea but not to a significant extent in normal uveal melanocytes.
  
Entity Tumorigenic progression in cell lines
Note Expression of the CapG protein was lost in the tumorigenic cell line, isolated from a human diploid fibroblast lineage. The CapG protein expression was lost also in cancer cell lines including stomach cancer, lung cancer and melanoma. The human stomach cancer cell line AZ 521 became non-tumorigenic after the introduction of CapG cDNA. Moreover, CapG expression was repressed in small-cell lung cancer tissues. These results indicate that CapG is a tumor suppressor gene involved in the tumorigenic progression of certain cancers.
  
  

External links

Nomenclature
HGNC (Hugo)CAPG   1474
Cards
AtlasCAPGID44449ch2p11
Entrez_Gene (NCBI)CAPG  822  capping protein (actin filament), gelsolin-like
GeneCards (Weizmann)CAPG
Ensembl hg19 (Hinxton)ENSG00000042493 [Gene_View]  chr2:85621871-85641197 [Contig_View]  CAPG [Vega]
Ensembl hg38 (Hinxton)ENSG00000042493 [Gene_View]  chr2:85621871-85641197 [Contig_View]  CAPG [Vega]
ICGC DataPortalENSG00000042493
cBioPortalCAPG
AceView (NCBI)CAPG
Genatlas (Paris)CAPG
WikiGenes822
SOURCE (Princeton)CAPG
Genomic and cartography
GoldenPath hg19 (UCSC)CAPG  -     chr2:85621871-85641197 -  2p11.2   [Description]    (hg19-Feb_2009)
GoldenPath hg38 (UCSC)CAPG  -     2p11.2   [Description]    (hg38-Dec_2013)
EnsemblCAPG - 2p11.2 [CytoView hg19]  CAPG - 2p11.2 [CytoView hg38]
Mapping of homologs : NCBICAPG [Mapview hg19]  CAPG [Mapview hg38]
OMIM153615   
Gene and transcription
Genbank (Entrez)AK130130 AK225374 AK225378 AK300507 AK309290
RefSeq transcript (Entrez)NM_001256139 NM_001256140 NM_001747
RefSeq genomic (Entrez)AC_000134 NC_000002 NC_018913 NT_022184 NW_001838769 NW_004929300
Consensus coding sequences : CCDS (NCBI)CAPG
Cluster EST : UnigeneHs.516155 [ NCBI ]
CGAP (NCI)Hs.516155
Alternative Splicing : Fast-db (Paris)GSHG0017770
Alternative Splicing GalleryENSG00000042493
Gene ExpressionCAPG [ NCBI-GEO ]     CAPG [ SEEK ]   CAPG [ MEM ]
SOURCE (Princeton)Expression in : [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
Protein : pattern, domain, 3D structure
UniProt/SwissProtP40121 (Uniprot)
NextProtP40121  [Medical]
With graphics : InterProP40121
Splice isoforms : SwissVarP40121 (Swissvar)
Domains : Interpro (EBI)ADF-H/Gelsolin-like_dom    Gelsolin-like_dom    Villin/Gelsolin   
Related proteins : CluSTrP40121
Domain families : Pfam (Sanger)Gelsolin (PF00626)   
Domain families : Pfam (NCBI)pfam00626   
Domain families : Smart (EMBL)GEL (SM00262)  
DMDM Disease mutations822
Blocks (Seattle)P40121
PDB (SRS)1J72    1JHW   
PDB (PDBSum)1J72    1JHW   
PDB (IMB)1J72    1JHW   
PDB (RSDB)1J72    1JHW   
Human Protein AtlasENSG00000042493
Peptide AtlasP40121
HPRD01088
IPIIPI00027341   IPI00908511   IPI00916783   IPI00972980   IPI00917562   IPI01011662   
Protein Interaction databases
DIP (DOE-UCLA)P40121
IntAct (EBI)P40121
FunCoupENSG00000042493
BioGRIDCAPG
IntegromeDBCAPG
STRING (EMBL)CAPG
Ontologies - Pathways
QuickGOP40121
Ontology : AmiGOactin binding  nucleus  nucleolus  nucleolus  cytoplasm  protein complex assembly  F-actin capping protein complex  cell projection assembly  nuclear membrane  melanosome  barbed-end actin filament capping  extracellular vesicular exosome  
Ontology : EGO-EBIactin binding  nucleus  nucleolus  nucleolus  cytoplasm  protein complex assembly  F-actin capping protein complex  cell projection assembly  nuclear membrane  melanosome  barbed-end actin filament capping  extracellular vesicular exosome  
Protein Interaction DatabaseCAPG
DoCM (Curated mutations)CAPG
Wikipedia pathwaysCAPG
Gene fusion - rearrangements
Polymorphisms : SNP, variants
NCBI Variation ViewerCAPG [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)CAPG
dbVarCAPG
ClinVarCAPG
1000_GenomesCAPG 
Exome Variant ServerCAPG
SNP (GeneSNP Utah)CAPG
SNP : HGBaseCAPG
Genetic variants : HAPMAPCAPG
Genomic VariantsCAPG  CAPG [DGVbeta]
Mutations
ICGC Data PortalENSG00000042493 
Somatic Mutations in Cancer : COSMICCAPG 
CONAN: Copy Number AnalysisCAPG 
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
Impact of mutations[PolyPhen2] [SIFT Human Coding SNP] [Buck Institute : MutDB] [Mutation Assessor] 
Diseases
DECIPHER (Syndromes)2:85621871-85641197
Mutations and Diseases : HGMDCAPG
OMIM153615   
MedgenCAPG
NextProtP40121 [Medical]
GENETestsCAPG
Disease Genetic AssociationCAPG
Huge Navigator CAPG [HugePedia]  CAPG [HugeCancerGEM]
snp3D : Map Gene to Disease822
DGIdb (Drug Gene Interaction db)CAPG
General knowledge
Homologs : HomoloGeneCAPG
Homology/Alignments : Family Browser (UCSC)CAPG
Phylogenetic Trees/Animal Genes : TreeFamCAPG
Chemical/Protein Interactions : CTD822
Chemical/Pharm GKB GenePA26056
Clinical trialCAPG
Cancer Resource (Charite)ENSG00000042493
Other databases
Other databasePSORTII
Probes
Litterature
PubMed37 Pubmed reference(s) in Entrez
CoreMineCAPG
GoPubMedCAPG
iHOPCAPG

Bibliography

Molecular cloning of human macrophage capping protein cDNA. A unique member of the gelsolin/villin family expressed primarily in macrophages.
Dabiri GA, Young CL, Rosenbloom J, Southwick FS.
J Biol Chem. 1992 Aug 15;267(23):16545-52.
PMID 1322908
 
The identification and differential expression of calcium-binding proteins associated with ocular melanoma.
Van Ginkel PR, Gee RL, Walker TM, Hu DN, Heizmann CW, Polans AS.
Biochim Biophys Acta. 1998 Dec 10;1448(2):290-7.
PMID 9920419
 
Cap G, a gelsolin family protein modulating protective effects of unidirectional shear stress.
Pellieux C, Desgeorges A, Pigeon CH, Chambaz C, Yin H, Hayoz D, Silacci P.
J Biol Chem. 2003 Aug 1;278(31):29136-44. Epub 2003 May 17.
PMID 12754261
 
Suppression of tumorigenicity, but not anchorage independence, of human cancer cells by new candidate tumor suppressor gene CapG.
Watari A, Takaki K, Higashiyama S, Li Y, Satomi Y, Takao T, Tanemura A, Yamaguchi Y, Katayama I, Shimakage M, Miyashiro I, Takami K, Kodama K, Yutsudo M.
Oncogene. 2006 Nov 30;25(56):7373-80. Epub 2006 Jun 12.
PMID 16767159
 
Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility.
Thompson CC, Ashcroft FJ, Patel S, Saraga G, Vimalachandran D, Prime W, Campbell F, Dodson A, Jenkins RE, Lemoine NR, Crnogorac-Jurcevic T, Yin HL, Costello E.
Gut. 2007 Jan;56(1):95-106. Epub 2006 Jul 17.
PMID 16847067
 
Clinical significance of gelsolin-like actin-capping protein expression in oral carcinogenesis: an immunohistochemical study of premalignant and malignant lesions of the oral cavity.
Nomura H, Uzawa K, Ishigami T, Kouzu Y, Koike H, Ogawara K, Siiba M, Bukawa H, Yokoe H, Kubosawa H, Tanzawa H.
BMC Cancer. 2008 Feb 1;8:39.
PMID 18237446
 
Four potential biomarkers as prognostic factors in stage III serous ovarian adenocarcinomas.
Partheen K, Levan K, Osterberg L, Claesson I, Fallenius G, Sundfeldt K, Horvath G.
Int J Cancer. 2008 Nov 1;123(9):2130-7.
PMID 18709641
 
Invasive breast cancer cells exhibit increased mobility of the actin-binding protein CapG.
Renz M, Betz B, Niederacher D, Bender HG, Langowski J.
Int J Cancer. 2008 Apr 1;122(7):1476-82.
PMID 18059028
 
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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Contributor(s)

Written11-2009Peeyush Kumar Singh, Ranjan Tamuli
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati-781 039, Assam, India

Citation

This paper should be referenced as such :
Singh, PK ; Tamuli, R
CAPG (capping protein (actin filament), gelsolin-like)
Atlas Genet Cytogenet Oncol Haematol. 2010;14(9):-.
Free online version   Free pdf version   [Bibliographic record ]
URL : http://AtlasGeneticsOncology.org/Genes/CAPGID44449ch2p11.html

© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Sun Dec 21 02:53:04 CET 2014

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