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CENPW (centromere protein W)

Written2013-06Seyoung Jeon, Soojin Lee
Department of Microbiology, Molecular Biology, Chungnam National Institute, 305-764, Daejeon, Republic of Korea

(Note : for Links provided by Atlas : click)

Identity

Alias_namesC6orf173
chromosome 6 open reading frame 173
Alias_symbol (synonym)CUG2
Other alias
HGNC (Hugo) CENPW
LocusID (NCBI) 387103
Atlas_Id 51452
Location 6q22.32  [Link to chromosome band 6q22]
Location_base_pair Starts at 126339789 and ends at 126348875 bp from pter ( according to hg19-Feb_2009)  [Mapping CENPW.png]
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)

DNA/RNA

 
  Figure 1. A genomic structure of the CENPW gene.
Description Exons: 3, coding exons: 3, introns: 2.
Transcription Transcript length: 750 bps, open reading frame: 264 bps.
Pseudogene No pseudogene.

Protein

 
  Figure 2. Schematic representation of CENPW protein. Each domain is represented with the unique color and name. One nuclear localization signal (NLS) sequence of 17 amino acids starts at amino acid 13.
Description CENP-W gene encodes 88-amino acid protein which shows ~27% of amino acid sequence similarity to a general transcription repressor, human DR1. It contains a typical NLS sequence and histone fold region which is critical for binding to centromeric DNA.
Expression Widely expressed; kidney, colon, breast, liver, lung, ovary, prostate, thyroid, etc.
Localisation Intracellular, nucleus, centromere, kinetochore, nucleus matrix, nucleolus.
Function CENP-W was originally identified as a cancer-upregulated gene 2 (CUG2) which is commonly overexpressed in various human cancer tissues. Although it has high oncogenic activities, CENP-W also induces cell apoptosis when overexpressed in certain cell lines. After it was revealed that CENP-W forms a stable heterodimer with CENP-T and is localized in kinetochores during mitosis, CENP-W become recognized as a new member of the inner centromere protein complex. Subsequent studies have also shown that CENP-T-W-S-X forms a unique centromeric nucleosome-like heterotetramer structure which binds to and supercoils DNA. As a nucleolus-associated protein, CENP-W may play critical role in the formation of functional kinetochore, possibly by facilitating the recruitment of other centromeric components during interphase, which is required for proper chromosome segregation during mitosis.
Homology According to NCBI-HomoloGene:
Pan troglodytes (chimpanzee): centromere protein W (NP_001012525.1, 88 aa)
Macacamulatta (Rhesus monkey): centromere protein W (XP_001107034.2, 88 aa)
Canis lupus familiaris (dog): centromere protein W-like (XP_003638832.1, 88 aa)
Bostaurus (cattle): centromere protein W (NP_001104731.1, 88 aa)
Mus musculus (house mouse): centromere protein W (NP_001103217.1, 86 aa)
Rattus norvegicus (Norway rat): centromere protein W (XP_001070657.1, 86 aa).

Mutations

Note Over expressed in various cancer tissues; notably high in the ovary (6.3-fold), liver (6.0), lung (4.9), and pancreas (3.8).
 
  Figure 3. mRNA expression of CUG2 in various tumors. DNA chip microarray profile in tumors (closed bar) and normal (open bar) tissues from the Gene Express Oncology DatasuiteTM of Gene Logic Inc.

Implicated in

Note
  
Entity Various human cancers
Oncogenesis CENP-W has been found to be upregulated in several tumor tissues. When the expression level of CENP-W was examined by DNA chip microarray and RT-PCR in tumors, six tissues (ovary, liver, lung, pancreas, colon, and stomach) showed high-fold increases in expression profiles. Also, CENP-W-transformed NIH3T3 cells exhibited exceptionally prominent cancerous phenotypes in vitro as well as in vivo tumor forming assays.
  

Bibliography

New centromeric component CENP-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein.
Chun Y, Park B, Koh W, Lee S, Cheon Y, Kim R, Che L, Lee S.
J Biol Chem. 2011 Dec 9;286(49):42758-69. doi: 10.1074/jbc.M111.228411. Epub 2011 Oct 14.
PMID 22002061
 
Cancer-upregulated gene 2 (CUG2), a new component of centromere complex, is required for kinetochore function.
Kim H, Lee M, Lee S, Park B, Koh W, Lee DJ, Lim DS, Lee S.
Mol Cells. 2009 Jun 30;27(6):697-701. doi: 10.1007/s10059-009-0083-2. Epub 2009 Jun 12.
PMID 19533040
 
Sp1 and Sp3 mediate basal and serum-induced expression of human CENP-W.
Kim H, Lee S, Park B, Che L, Lee S.
Mol Biol Rep. 2010 Oct;37(7):3593-600. doi: 10.1007/s11033-010-0008-3. Epub 2010 Feb 24.
PMID 20180024
 
Cug2 is essential for normal mitotic control and CNS development in zebrafish.
Kim HT, So JH, Jung SH, Ahn DG, Koh W, Kim NS, Kim SH, Lee S, Kim CH.
BMC Dev Biol. 2011 Aug 15;11:49. doi: 10.1186/1471-213X-11-49.
PMID 21838932
 
Molecular cloning and functional analysis of a novel oncogene, cancer-upregulated gene 2 (CUG2).
Lee S, Gang J, Jeon SB, Choo SH, Lee B, Kim YG, Lee YS, Jung J, Song SY, Koh SS.
Biochem Biophys Res Commun. 2007 Aug 31;360(3):633-9. Epub 2007 Jun 28.
PMID 17610844
 
Cancer-upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV-3 cells.
Lee S, Koh W, Kim HT, Kim CH, Lee S.
Cell Biochem Funct. 2010 Aug;28(6):461-8. doi: 10.1002/cbf.1678.
PMID 20648695
 
CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold.
Nishino T, Takeuchi K, Gascoigne KE, Suzuki A, Hori T, Oyama T, Morikawa K, Cheeseman IM, Fukagawa T.
Cell. 2012 Feb 3;148(3):487-501. doi: 10.1016/j.cell.2011.11.061.
PMID 22304917
 
CUG2, a novel oncogene confers reoviral replication through Ras and p38 signaling pathway.
Park EH, Park EH, Cho IR, Srisuttee R, Min HJ, Oh MJ, Jeong YJ, Jhun BH, Johnston RN, Lee S, Koh SS, Chung YH.
Cancer Gene Ther. 2010 May;17(5):307-14. doi: 10.1038/cgt.2009.83. Epub 2010 Jan 15.
PMID 20075984
 
Premitotic assembly of human CENPs -T and -W switches centromeric chromatin to a mitotic state.
Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan KF.
PLoS Biol. 2011 Jun;9(6):e1001082. doi: 10.1371/journal.pbio.1001082. Epub 2011 Jun 14.
PMID 21695110
 

Citation

This paper should be referenced as such :
Jeon, S ; Lee, S
CENPW (centromere protein W)
Atlas Genet Cytogenet Oncol Haematol. 2014;18(1):5-7.
Free journal version : [ pdf ]   [ DOI ]
On line version : http://AtlasGeneticsOncology.org/Genes/CENPWID51452ch6q22.html


External links

Nomenclature
HGNC (Hugo)CENPW   21488
Cards
AtlasCENPWID51452ch6q22
Entrez_Gene (NCBI)CENPW  387103  centromere protein W
AliasesC6orf173; CENP-W; CUG2
GeneCards (Weizmann)CENPW
Ensembl hg19 (Hinxton)ENSG00000203760 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000203760 [Gene_View]  chr6:126339789-126348875 [Contig_View]  CENPW [Vega]
ICGC DataPortalENSG00000203760
TCGA cBioPortalCENPW
AceView (NCBI)CENPW
Genatlas (Paris)CENPW
WikiGenes387103
SOURCE (Princeton)CENPW
Genetics Home Reference (NIH)CENPW
Genomic and cartography
GoldenPath hg38 (UCSC)CENPW  -     chr6:126339789-126348875 +  6q22.32   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)CENPW  -     6q22.32   [Description]    (hg19-Feb_2009)
EnsemblCENPW - 6q22.32 [CytoView hg19]  CENPW - 6q22.32 [CytoView hg38]
Mapping of homologs : NCBICENPW [Mapview hg19]  CENPW [Mapview hg38]
OMIM611264   
Gene and transcription
Genbank (Entrez)AI207187 AL832235 AY902475 BC017928 BC039556
RefSeq transcript (Entrez)NM_001012507 NM_001286524 NM_001286525
RefSeq genomic (Entrez)
Consensus coding sequences : CCDS (NCBI)CENPW
Cluster EST : UnigeneHs.486401 [ NCBI ]
CGAP (NCI)Hs.486401
Alternative Splicing GalleryENSG00000203760
Gene ExpressionCENPW [ NCBI-GEO ]   CENPW [ EBI - ARRAY_EXPRESS ]   CENPW [ SEEK ]   CENPW [ MEM ]
Gene Expression Viewer (FireBrowse)CENPW [ Firebrowse - Broad ]
SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
GenevisibleExpression in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)387103
GTEX Portal (Tissue expression)CENPW
Human Protein AtlasENSG00000203760-CENPW [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ5EE01   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtQ5EE01  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProQ5EE01
Splice isoforms : SwissVarQ5EE01
PhosPhoSitePlusQ5EE01
Domains : Interpro (EBI)CENP-W    Histone-fold   
Domain families : Pfam (Sanger)CENP-W (PF15510)   
Domain families : Pfam (NCBI)pfam15510   
Conserved Domain (NCBI)CENPW
DMDM Disease mutations387103
Blocks (Seattle)CENPW
SuperfamilyQ5EE01
Human Protein Atlas [tissue]ENSG00000203760-CENPW [tissue]
Peptide AtlasQ5EE01
HPRD16660
IPIIPI00554528   IPI00645095   IPI00640704   
Protein Interaction databases
DIP (DOE-UCLA)Q5EE01
IntAct (EBI)Q5EE01
FunCoupENSG00000203760
BioGRIDCENPW
STRING (EMBL)CENPW
ZODIACCENPW
Ontologies - Pathways
QuickGOQ5EE01
Ontology : AmiGOmitotic cell cycle  chromosome, centromeric region  kinetochore  condensed chromosome kinetochore  DNA binding  protein binding  nucleoplasm  nucleoplasm  nucleolus  chromosome segregation  chromosome segregation  nuclear matrix  CENP-A containing nucleosome assembly  protein heterodimerization activity  chromosome organization  cell division  kinetochore assembly  
Ontology : EGO-EBImitotic cell cycle  chromosome, centromeric region  kinetochore  condensed chromosome kinetochore  DNA binding  protein binding  nucleoplasm  nucleoplasm  nucleolus  chromosome segregation  chromosome segregation  nuclear matrix  CENP-A containing nucleosome assembly  protein heterodimerization activity  chromosome organization  cell division  kinetochore assembly  
REACTOMEQ5EE01 [protein]
REACTOME PathwaysR-HSA-606279 [pathway]   
NDEx NetworkCENPW
Atlas of Cancer Signalling NetworkCENPW
Wikipedia pathwaysCENPW
Orthology - Evolution
OrthoDB387103
GeneTree (enSembl)ENSG00000203760
Phylogenetic Trees/Animal Genes : TreeFamCENPW
HOVERGENQ5EE01
HOGENOMQ5EE01
Homologs : HomoloGeneCENPW
Homology/Alignments : Family Browser (UCSC)CENPW
Gene fusions - Rearrangements
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerCENPW [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)CENPW
dbVarCENPW
ClinVarCENPW
1000_GenomesCENPW 
Exome Variant ServerCENPW
ExAC (Exome Aggregation Consortium)ENSG00000203760
GNOMAD BrowserENSG00000203760
Genetic variants : HAPMAP387103
Genomic Variants (DGV)CENPW [DGVbeta]
DECIPHERCENPW [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisCENPW 
Mutations
ICGC Data PortalCENPW 
TCGA Data PortalCENPW 
Broad Tumor PortalCENPW
OASIS PortalCENPW [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICCENPW  [overview]  [genome browser]  [tissue]  [distribution]  
Mutations and Diseases : HGMDCENPW
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD - Leiden Open Variation Database
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
BioMutasearch CENPW
DgiDB (Drug Gene Interaction Database)CENPW
DoCM (Curated mutations)CENPW (select the gene name)
CIViC (Clinical Interpretations of Variants in Cancer)CENPW (select a term)
intoGenCENPW
NCG5 (London)CENPW
Cancer3DCENPW(select the gene name)
Impact of mutations[PolyPhen2] [SIFT Human Coding SNP] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
Diseases
OMIM611264   
Orphanet
MedgenCENPW
Genetic Testing Registry terí=CANPW TARGET=GTR>CENPW
NextProtQ5EE01 [Medical]
TSGene387103
GENETestsCENPW
Target ValidationCENPW
Huge Navigator CENPW [HugePedia]
snp3D : Map Gene to Disease387103
BioCentury BCIQCENPW
ClinGenCENPW
Clinical trials, drugs, therapy
Chemical/Protein Interactions : CTD387103
Chemical/Pharm GKB GenePA165617841
Clinical trialCENPW
Miscellaneous
canSAR (ICR)CENPW (select the gene name)
Probes
Litterature
PubMed33 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
CoreMineCENPW
EVEXCENPW
GoPubMedCENPW
iHOPCENPW
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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indexed on : Thu Oct 12 16:18:49 CEST 2017

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