| Description | 357 amino acids; 37,563.75 Da; Isoelectric point: 5.122; Mov34/MPN/PAD1 domain. |
| Expression | Ubiquitous. |
| Localisation | Cytoplasmic. There is also evidence of its existence in the nucleus. |
| Function | eIF3f is a subunit of eIF3 complex and is highly conserved among species. eIF3f interacts with the caspase-processed isoform of CDK11 (CDK11p46), which appears to be a down-stream effector in apoptotic signaling. eIF3f can be phosphorylated at Ser46 and Thr119 by CDK11p46 during apoptosis. The phosphorylation of eIF3f contributes to translation inhibition and apoptosis. The expression of eIF3f is significantly decreased in pancreatic cancer and melanoma. Loss of the eIF3f allele has been reported in pancreatic cancer and melanoma. Ectopic expression of eIF3f causes rRNA degradation, inhibits translation and cell proliferation, and induces apoptosis in pancreatic cancer and melanoma cells. On the other hand, knockdown of eIF3f prevents apoptosis in pancreatic cancer and melanoma cells. Recent studies also showed decreased cell growth, cell proliferation, colony formation and increased apoptosis in eIF3f-overexpressing NIH3T3 cells. |
| Homology | eIF3f is not found in S. cerevisiae; However it is found in Schizosaccharomyces pombe (SPBC4C3.07, 35% identity) and in Drosophila Melanogaster (CG9769, 49% identity). |
| Loss of the eukaryotic initiation factor 3f in melanoma. |
| Doldan A, Chandramouli A, Shanas R, Bhattacharyya A, Leong SP, Nelson MA, Shi J. |
| Mol Carcinog. 2008 Oct;47(10):806-13. |
| PMID 18381585 |
| |
| All translation elongation factors and the e, f, and h subunits of translation initiation factor 3 are encoded by 5'-terminal oligopyrimidine (TOP) mRNAs. |
| Iadevaia V, Caldarola S, Tino E, Amaldi F, Loreni F. |
| RNA. 2008 Sep;14(9):1730-6. Epub 2008 Jul 24. |
| PMID 18658124 |
| |
| mRNA retrotransposition coupled with 5' inversion as a possible source of new genes. |
| Kojima KK, Okada N. |
| Mol Biol Evol. 2009 Jun;26(6):1405-20. Epub 2009 Mar 16. |
| PMID 19289598 |
| |
| The initiation factor eIF3-f is a major target for atrogin1/MAFbx function in skeletal muscle atrophy. |
| Lagirand-Cantaloube J, Offner N, Csibi A, Leibovitch MP, Batonnet-Pichon S, Tintignac LA, Segura CT, Leibovitch SA. |
| EMBO J. 2008 Apr 23;27(8):1266-76. Epub 2008 Mar 20. |
| PMID 18354498 |
| |
| Reconstitution reveals the functional core of mammalian eIF3. |
| Masutani M, Sonenberg N, Yokoyama S, Imataka H. |
| EMBO J. 2007 Jul 25;26(14):3373-83. Epub 2007 Jun 21. |
| PMID 17581632 |
| |
| The p34cdc2-related cyclin-dependent kinase 11 interacts with the p47 subunit of eukaryotic initiation factor 3 during apoptosis. |
| Shi J, Feng Y, Goulet AC, Vaillancourt RR, Sachs NA, Hershey JW, Nelson MA. |
| J Biol Chem. 2003 Feb 14;278(7):5062-71. Epub 2002 Nov 20. |
| PMID 12446680 |
| |
| Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis. |
| Shi J, Hershey JW, Nelson MA. |
| FEBS Lett. 2009 Mar 18;583(6):971-7. Epub 2009 Feb 24. |
| PMID 19245811 |
| |
| Decreased expression of eukaryotic initiation factor 3f deregulates translation and apoptosis in tumor cells. |
| Shi J, Kahle A, Hershey JW, Honchak BM, Warneke JA, Leong SP, Nelson MA. |
| Oncogene. 2006 Aug 10;25(35):4923-36. Epub 2006 Mar 13. |
| PMID 16532022 |
| |
| Inhibition of HIV-1 replication by eIF3f. |
| Valente ST, Gilmartin GM, Mott C, Falkard B, Goff SP. |
| Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4071-8. Epub 2009 Feb 23. |
| PMID 19237569 |
| |
