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| | Shematic structures of HSP105alpha and HSP105beta proteins. Shaded box represents the spliced out region of HSP105alpha which is lacking in HSP105beta. |
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| Description | Hsp105alpha: 858 amino acids, 105 kDa; contains an ATP binding domain (residues 1-383), b-sheet domain (residues 384-511), loop domain (residues 512-607) and alpha-helix domain (residues 608-858). Hsp105beta: 814 amino acids, 90 kDa; contains an ATP binding domain (residues 1-383), b-sheet domain (residues 384-511), loop domain (residues 512-563) and alpha-helix domain (residues 564-814). |
| Expression | wide, highly expressed in brain |
| Localisation | Hsp105alpha, cytoplasmic; Hsp105beta, nuclear |
| Function | Hsp105alpha and Hsp105beta suppress the aggregation of denatured proteins; function as a substitute for Hsp70 family proteins to suppress the aggregation of denatured proteins in cells under severe stress; regulate substrate binding cycle of Hsp70/Hsc70 by inhibiting the ATPase activity of Hsp70/Hsc70. |
| Homology | With mouse apg-1, mouse apg-2, sea urchin egg receptor, C. elegans 86.9-kDa protein, A. thaliana hsp91 and S. cerevisiae SSE1, human hsp70 and human hsc70. |
| Synthetic small interfering RNA targeting heat shock protein 105 induces apoptosis of various cancer cells both in vitro and in vivo. |
| Hosaka S, Nakatsura T, Tsukamoto H, Hatayama T, Baba H, Nishimura Y |
| Cancer science. 2006 ; 97 (7) : 623-632. |
| PMID 16827803 |
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| Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105. |
| Ishihara K, Yasuda K, Hatayama T |
| Biochimica et biophysica acta. 1999 ; 1444 (1) : 138-142. |
| PMID 9931472 |
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| Heat shock protein 105 is overexpressed in a variety of human tumors. |
| Kai M, Nakatsura T, Egami H, Senju S, Nishimura Y, Ogawa M |
| Oncology reports. 2003 ; 10 (6) : 1777-1782. |
| PMID 14534695 |
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| DNA vaccination of HSP105 leads to tumor rejection of colorectal cancer and melanoma in mice through activation of both CD4 T cells and CD8 T cells. |
| Miyazaki M, Nakatsura T, Yokomine K, Senju S, Monji M, Hosaka S, Komori H, Yoshitake Y, Motomura Y, Minohara M, Kubo T, Ishihara K, Hatayama T, Ogawa M, Nishimura Y |
| Cancer science. 2005 ; 96 (10) : 695-705. |
| PMID 16232202 |
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| Heat shock protein 105 is overexpressed in squamous cell carcinoma and extramammary Paget disease but not in basal cell carcinoma. |
| Muchemwa FC, Nakatsura T, Ihn H, Kageshita T |
| The British journal of dermatology. 2006 ; 155 (3) : 582-585. |
| PMID 16911285 |
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| Gene cloning of immunogenic antigens overexpressed in pancreatic cancer. |
| Nakatsura T, Senju S, Yamada K, Jotsuka T, Ogawa M, Nishimura Y |
| Biochemical and biophysical research communications. 2001 ; 281 (4) : 936-944. |
| PMID 11237751 |
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| Hsp105 family proteins suppress staurosporine-induced apoptosis by inhibiting the translocation of Bax to mitochondria in HeLa cells. |
| Yamagishi N, Ishihara K, Saito Y, Hatayama T |
| Experimental cell research. 2006 ; 312 (17) : 3215-3223. |
| PMID 16857185 |
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| Genomic cloning and promoter analysis of the mouse 105-kDa heat shock protein (HSP105) gene. |
| Yasuda K, Ishihara K, Nakashima K, Hatayama T |
| Biochemical and biophysical research communications. 1999 ; 256 (1) : 75-80. |
| PMID 10066425 |
| |
| Cloning and expression of murine high molecular mass heat shock proteins, HSP105. |
| Yasuda K, Nakai A, Hatayama T, Nagata K |
| The Journal of biological chemistry. 1995 ; 270 (50) : 29718-29723. |
| PMID 8530361 |
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