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SPA17 (sperm autoantigenic protein 17)

Written2007-09Leri S. Faried, Ahmad Faried
Department of Gynecology, Reproductive Medicine, Graduate School of Medicine, Gunma University, Japan (FLS); Department of Surgery I, Gunma University, Japan (FA).

(Note : for Links provided by Atlas : click)

Identity

Alias_symbol (synonym)SP17
CT22
Other aliasSP17-1
HGNC (Hugo) SPA17
LocusID (NCBI) 53340
Atlas_Id 42360
Location 11q24.2  [Link to chromosome band 11q24]
Location_base_pair Starts at 124673844 and ends at 124694791 bp from pter ( according to hg19-Feb_2009)  [Mapping SPA17.png]
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)

DNA/RNA

Description The SPA17 gene contains 5 exons and 4 introns and was predicted to span over approximately 23 kb of the genomic DNA. The first exon is encodes solely the 5' untranslated sequence. The exon 2 encodes the first 51 amino acid residue. Exon 3 and 4 are only 71 and 87 bp length and contain coding region. Exon 5 contains stop codon and followed by 3' untranslated sequence. The size of introns 2, 3 and 4 were 5970, 10.235 and 2520 bp, respectively.
Transcription Two transcript variants encoding the same protein have been identified for SP17-1 gene. This variant which was named SP17-1A and SP17-1B, both cDNA encodes identical protein sequence and only differed in 5' UTR part. One hundred percent homology can be detected from the poly A tail up to SP17-1 position -62 for variant A and -31 for variant B.
Pseudogene There is one pseudogene, SP17-2 intronless gene has all the hallmark of retro-position giving rise to a pseudogene, located on chromosome 10. SP17-2 gene had 61 point mutations, 4 nucleotide insertions and 2 deletions leading to internal stop codons and frame shifts which do not result in ORF encoding a full-length SPA17 polypeptide.

Protein

Description The SPA17 consists of 151 amino acids corresponding to a molecular weight of 17.4 kDa. SPA17 protein indicated to have functional role in cell regulation by its calmodulin binding site located at its C-terminal end. The N-terminal end of SPA17, similar to human cyclic adenosine 3'-5'-monophosphate (cAMP)-dependent protein kinase type II regulatory subunits (RII), may facilitate dimmer formation. SPA17's central domain is necessary for heparin-binding and exhibits the greatest sequence divergence of all three domains. This region plays an important role in cell-cell and/or cell matrix recognition process and/or cell regulation, possibly via its interaction with extra cellular heparin sulfate.
Expression The SPA17 expression was originally found to be expressed exclusively in the testis. Recently, this gene also found in other somatic tissues.
Localisation SPA17 is present throughout the cytoplasm and is particularly concentrated around the nucleus. It's also present on the surface of B-lymphocytes and granulocytes; T-lymphocytes and monocytes; most malignant B and T lymphocytes cell lines.
Function SPA17 is highly antigenic, testes specific antigen and known have function to bind sperm to the zona pellucida of the oocyte. In addition, SPA17 may also have an alternative role in highly proliferating and neoplastic cells, such as a mediator of signal transduction, protein synthesis and/or unregulated growth.
Homology The homologues of SPA17 protein sequences were found from eutherian and marsupial mammals, including rat, mouse, macaque, baboon, human, marmoset, rabbit, Monodelphis and wallaby.

Implicated in

Note
  
Entity Immune system
Disease SPA17 is a highly immunogenic protein. More than 90% of male spontaneously developed B-cell immunity of SPA17 following vasectomy. SPA17 can be easily generated SPA17-specific cytotoxic T lymphocytes (CTLs) from peripheral blood of healthy donors and also from peripheral blood of cancer-bearing patients. SPA17 have been tested as tumor vaccines in a phase I study. In addition, SPA17 gene was described in human rheumatoid arthritis (RA).
  
  
Entity Malignancy
Disease SPA17 reported to be over-expressed in female reproduction organ, multiple myeloma, esthesioneuroblastoma, nervous system tumors and esophageal cancer.
Prognosis SPA17 expression closely related with poor prognosis in malignancy.
Oncogenesis SPA17 was identified as a candidate gene related to chemoresistance of clear cell adenocarcinoma of the ovary, which has a poor prognosis due to its chemoresistancy and early metastasis to the lymph nodes.
  

Bibliography

Genomic organization of an intron-containing sperm protein 17 gene (Sp17-1) and an intronless pseudogene (Sp17-2) in humans: a new model.
Buchli R, De Jong A, Robbins DL
Biochimica et biophysica acta. 2002 ; 1578 (1-3) : 29-42.
PMID 12393185
 
Sperm protein 17 expression defines 2 subsets of primary esthesioneuroblastoma.
Bumm K, Grizzi F, Franceschini B, Koch M, Iro H, Wurm J, Ceva-Grimaldi G, Dimmler A, Cobos E, Dioguardi N, Sinha UK, Kast WM, Chiriva-Internati M
Human pathology. 2005 ; 36 (12) : 1289-1293.
PMID 16311122
 
Successful generation of sperm protein 17 (Sp17)-specific cytotoxic T lymphocytes from normal donors: implication for tumour-specific adoptive immunotherapy following allogeneic stem cell transplantation for Sp17-positive multiple myeloma.
Chiriva-Internati M, Wang Z, Salati E, Wroblewski D, Lim SH
Scandinavian journal of immunology. 2002 ; 56 (4) : 429-433.
PMID 12234264
 
Characterization of sperm protein 17 in human somatic and neoplastic tissue.
De Jong A, Buchli R, Robbins D
Cancer letters. 2002 ; 186 (2) : 201-209.
PMID 12213290
 
Sperm protein 17 is expressed in human nervous system tumours.
Grizzi F, Gaetani P, Franceschini B, Di Ieva A, Colombo P, Ceva-Grimaldi G, Bollati A, Frezza EE, Cobos E, Rodriguez y Baena R, Dioguardi N, Chiriva-Internati M
BMC cancer. 2006 ; 6 : page 23.
PMID 16438728
 
Clinical significance of sperm protein 17 expression and immunogenicity in esophageal cancer.
Gupta G, Sharma R, Chattopadhyay TK, Gupta SD, Ralhan R
International journal of cancer. Journal international du cancer. 2007 ; 120 (8) : 1739-1747.
PMID 17230514
 
Sperm protein 17 is expressed on normal and malignant lymphocytes and promotes heparan sulfate-mediated cell-cell adhesion.
Lacy HM, Sanderson RD
Blood. 2001 ; 98 (7) : 2160-2165.
PMID 11568003
 
Autoimmunogenicity of the human sperm protein Sp17 in vasectomized men and identification of linear B cell epitopes.
Lea IA, Adoyo P, O'Rand MG
Fertility and sterility. 1997 ; 67 (2) : 355-361.
PMID 9022615
 
Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17.
Lea IA, Richardson RT, Widgren EE, O'Rand MG
Biochimica et biophysica acta. 1996 ; 1307 (3) : 263-266.
PMID 8688458
 
Idiotypic protein-pulsed dendritic cell vaccination in multiple myeloma.
Lim SH, Bailey-Wood R
International journal of cancer. Journal international du cancer. 1999 ; 83 (2) : 215-222.
PMID 10471530
 
Sperm protein 17 is a novel cancer-testis antigen in multiple myeloma.
Lim SH, Wang Z, Chiriva-Internati M, Xue Y
Blood. 2001 ; 97 (5) : 1508-1510.
PMID 11222401
 
Sperm protein 17 influences the tissue-specific malignancy of clear cell adenocarcinoma in human epithelial ovarian cancer.
Nakazato T, Kanuma T, Tamura T, Faried LS, Aoki H, Minegishi T
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society. 2007 ; 17 (2) : 426-432.
PMID 17309563
 
Characterization of the rabbit sperm membrane autoantigen, RSA, as a lectin-like zona binding protein.
O'Rand MG, Widgren EE, Fisher SJ
Developmental biology. 1988 ; 129 (1) : 231-240.
PMID 3410159
 
Expression of sperm protein 17 (Sp17) in ovarian cancer.
Straughn JM Jr, Shaw DR, Guerrero A, Bhoola SM, Racelis A, Wang Z, Chiriva-Internati M, Grizzle WE, Alvarez RD, Lim SH, Strong TV
International journal of cancer. Journal international du cancer. 2004 ; 108 (6) : 805-811.
PMID 14712480
 
cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes.
Taylor SS, Buechler JA, Yonemoto W
Annual review of biochemistry. 1990 ; 59 : 971-1005.
PMID 2165385
 
Characterization of Sp17: a ubiquitous three domain protein that binds heparin.
Wen Y, Richardson RT, Widgren EE, O'Rand MG
The Biochemical journal. 2001 ; 357 (Pt 1) : 25-31.
PMID 11415432
 

Citation

This paper should be referenced as such :
Faried, LS ; Faried, A
SPA17 (sperm autoantigenic protein 17)
Atlas Genet Cytogenet Oncol Haematol. 2008;12(2):159-160.
Free journal version : [ pdf ]   [ DOI ]
On line version : http://AtlasGeneticsOncology.org/Genes/SPA17ID42360ch11q24.html


External links

Nomenclature
HGNC (Hugo)SPA17   11210
Cards
AtlasSPA17ID42360ch11q24
Entrez_Gene (NCBI)SPA17  53340  sperm autoantigenic protein 17
AliasesCT22; SP17; SP17-1
GeneCards (Weizmann)SPA17
Ensembl hg19 (Hinxton)ENSG00000064199 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000064199 [Gene_View]  chr11:124673844-124694791 [Contig_View]  SPA17 [Vega]
ICGC DataPortalENSG00000064199
TCGA cBioPortalSPA17
AceView (NCBI)SPA17
Genatlas (Paris)SPA17
WikiGenes53340
SOURCE (Princeton)SPA17
Genetics Home Reference (NIH)SPA17
Genomic and cartography
GoldenPath hg38 (UCSC)SPA17  -     chr11:124673844-124694791 +  11q24.2   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)SPA17  -     11q24.2   [Description]    (hg19-Feb_2009)
EnsemblSPA17 - 11q24.2 [CytoView hg19]  SPA17 - 11q24.2 [CytoView hg38]
Mapping of homologs : NCBISPA17 [Mapview hg19]  SPA17 [Mapview hg38]
OMIM608621   
Gene and transcription
Genbank (Entrez)AF334735 AK307271 AK311806 BC019224 BC032457
RefSeq transcript (Entrez)NM_017425
RefSeq genomic (Entrez)
Consensus coding sequences : CCDS (NCBI)SPA17
Cluster EST : UnigeneHs.286233 [ NCBI ]
CGAP (NCI)Hs.286233
Alternative Splicing GalleryENSG00000064199
Gene ExpressionSPA17 [ NCBI-GEO ]   SPA17 [ EBI - ARRAY_EXPRESS ]   SPA17 [ SEEK ]   SPA17 [ MEM ]
Gene Expression Viewer (FireBrowse)SPA17 [ Firebrowse - Broad ]
SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
GenevestigatorExpression in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)53340
GTEX Portal (Tissue expression)SPA17
Human Protein AtlasENSG00000064199-SPA17 [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ15506   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtQ15506  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProQ15506
Splice isoforms : SwissVarQ15506
PhosPhoSitePlusQ15506
Domaine pattern : Prosite (Expaxy)IQ (PS50096)   
Domains : Interpro (EBI)cAMP_dep_PK_reg_su_I/II_a/b    IQ_motif_EF-hand-BS    Sp17   
Domain families : Pfam (Sanger)IQ (PF00612)    RIIa (PF02197)   
Domain families : Pfam (NCBI)pfam00612    pfam02197   
Domain families : Smart (EMBL)IQ (SM00015)  RIIa (SM00394)  
Conserved Domain (NCBI)SPA17
DMDM Disease mutations53340
Blocks (Seattle)SPA17
SuperfamilyQ15506
Human Protein Atlas [tissue]ENSG00000064199-SPA17 [tissue]
Peptide AtlasQ15506
HPRD12272
IPIIPI00017966   
Protein Interaction databases
DIP (DOE-UCLA)Q15506
IntAct (EBI)Q15506
FunCoupENSG00000064199
BioGRIDSPA17
STRING (EMBL)SPA17
ZODIACSPA17
Ontologies - Pathways
QuickGOQ15506
Ontology : AmiGOepithelial cilium movement  protein binding  cytoplasm  cilium  spermatogenesis  single fertilization  binding of sperm to zona pellucida  external side of plasma membrane  motile cilium  sperm fibrous sheath  sperm principal piece  
Ontology : EGO-EBIepithelial cilium movement  protein binding  cytoplasm  cilium  spermatogenesis  single fertilization  binding of sperm to zona pellucida  external side of plasma membrane  motile cilium  sperm fibrous sheath  sperm principal piece  
NDEx NetworkSPA17
Atlas of Cancer Signalling NetworkSPA17
Wikipedia pathwaysSPA17
Orthology - Evolution
OrthoDB53340
GeneTree (enSembl)ENSG00000064199
Phylogenetic Trees/Animal Genes : TreeFamSPA17
HOVERGENQ15506
HOGENOMQ15506
Homologs : HomoloGeneSPA17
Homology/Alignments : Family Browser (UCSC)SPA17
Gene fusions - Rearrangements
Tumor Fusion PortalSPA17
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerSPA17 [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)SPA17
dbVarSPA17
ClinVarSPA17
1000_GenomesSPA17 
Exome Variant ServerSPA17
ExAC (Exome Aggregation Consortium)ENSG00000064199
GNOMAD BrowserENSG00000064199
Genetic variants : HAPMAP53340
Genomic Variants (DGV)SPA17 [DGVbeta]
DECIPHERSPA17 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisSPA17 
Mutations
ICGC Data PortalSPA17 
TCGA Data PortalSPA17 
Broad Tumor PortalSPA17
OASIS PortalSPA17 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICSPA17  [overview]  [genome browser]  [tissue]  [distribution]  
Mutations and Diseases : HGMDSPA17
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
BioMutasearch SPA17
DgiDB (Drug Gene Interaction Database)SPA17
DoCM (Curated mutations)SPA17 (select the gene name)
CIViC (Clinical Interpretations of Variants in Cancer)SPA17 (select a term)
intoGenSPA17
NCG5 (London)SPA17
Cancer3DSPA17(select the gene name)
Impact of mutations[PolyPhen2] [SIFT Human Coding SNP] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
Diseases
OMIM608621   
Orphanet
DisGeNETSPA17
MedgenSPA17
Genetic Testing Registry SPA17
NextProtQ15506 [Medical]
TSGene53340
GENETestsSPA17
Target ValidationSPA17
Huge Navigator SPA17 [HugePedia]
snp3D : Map Gene to Disease53340
BioCentury BCIQSPA17
ClinGenSPA17
Clinical trials, drugs, therapy
Chemical/Protein Interactions : CTD53340
Chemical/Pharm GKB GenePA36047
Clinical trialSPA17
Miscellaneous
canSAR (ICR)SPA17 (select the gene name)
Probes
Litterature
PubMed34 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
CoreMineSPA17
EVEXSPA17
GoPubMedSPA17
iHOPSPA17
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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