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SPARC (secreted protein acidic and cysteine-rich)

Written2016-12Ana C. Pavanelli, Flávia Regina Mangone, Maria A. Nagai.
Discipline of Oncology, Department of Radiology and Oncology, Faculty of Medicine, University of São Paulo, 01246-903, São Paulo, and Laboratory of Molecular Genetics, Center for Translational Research in Oncology, Cancer Institute of the State of ão Paulo (ICESP), 01246-000, São Paulo, Brazil;

Abstract Review on SPARC, with data on DNA, on the protein encoded, and where the gene is implicated.

Keywords SPARC; cChromosome 5; Matricellular glycoprotein; Osteogenesis Imperfecta; Osteoporosis; Pulmonary Fibrosis Cardiac Fibrosis; Breast cancer.

(Note : for Links provided by Atlas : click)


Alias (NCBI)Secreted Protein, Acidic
Cysteine-Rich (Osteonectin)
Basement-Membrane Protein 40
Osteonectin; BM-40; ON
Secreted Protein Acidic And Rich In Cysteine
Cysteine-Rich Protein
HGNC Alias symbONT
HGNC Alias namecysteine-rich protein
 secreted protein acidic and rich in cysteine
HGNC Previous nameON
HGNC Previous nameosteonectin
LocusID (NCBI) 6678
Atlas_Id 42369
Location 5q31.3-q32  [Link to chromosome band 5q31]
Location_base_pair Starts at 151661096 and ends at 151686915 bp from pter ( according to GRCh38/hg38-Dec_2013)  [Mapping SPARC.png]
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)


Description DNA size: 26,070 kb; Exon count: 10; mRNA size: 3604 bp NM_003118. A CPG-rich sequence has been identified at the 5' region of the SPARC gene, characterizing the presence of CpG island spanning from exon 1 to intron 1 (Yang et al., 2007).
Transcription Three transcript variants encoding different isoforms have been found for this gene.
NM_003118.3 - Homo sapiens secreted protein acidic and cysteine-rich (SPARC), transcript variant 1, mRNA: NP_003109.1. Transcript size: 3604 bp. Variant 1 encodes the predominant isoform.
NM_001309443.1 - Homo sapiens secreted protein acidic and cysteine-rich (SPARC), transcript variant 2, mRNA: NP_001296372.1. Transcript size: 3601 bp. Variant 2 uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform has the same N- and C-termini but is 1 aa shorter compared to isoform 1.
NM_001309444.1 - Homo sapiens secreted protein acidic and cysteine-rich (SPARC), transcript variant 3, mRNA: NP_001296373.1 . Transcript size: 3602 bp. Variant 3 uses an alternate splice junction at the 5' end of the last exon compared to variant 1 that causes a frameshift. The resulting isoform has a longer and distinct C-terminus compared to isoform 1.


Note SPARC encodes a cysteine-rich acidic matrix-associated protein that belongs to a family of SPARC-related proteins composed of others six members, that include SPOCK1, SPOCK2, SPOCK3, SPARCL1, SMOC1, SMOC2 (testican-1, -2, -3, SPARC-like 1 (or hevin, Mast9), and SPARC-related modular calcium binding (SMOC)-1, and -2). All members of this protein family share the three similar domains (Bradshaw and Sage, 2001; Brekken and Sage, 2000). SPARC protein is required for the collagen in the bone to become calcified. SPARC is also involved in extracellular matrix synthesis and remodeling being associated with the promotion of changes in cell shape. SPARC protein has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion (GeneCard RefSeq).
molecular weight: 303aa, 43 kDa; Isoelectric point: 4.4719. Although, after cleavage of the signal sequence, SPARC is a 32-kDa protein (Mason et al. 1986), the secreted form is identified as a 43 kDa protein on SDS-PAGE, which is due to the addition of carbohydrate (Sage et al. 1984).
NP_003109.1: molecular weight: 303 aa; NP_001296372.1: molecular weight: 302 aa; NP_001296373.1: molecular weight: 341 aa
  Figure 1 - Schematic representation of the 303 aa human SPARC protein with its functional and structural domains. Box represents the three functional domains: acidic domain (18-52aa), follistatin-like (53-137aa), extracellular Ca2+-binding (138- 286aa). The first seventeen amino acids correspond to the signaling peptide. Stars and triangles represent some of the structural domains: yellow and green stars represent collagen-binding and high-affinity Ca2+-binding residues, respectively. Triangles represent cleavage sites for cathepsin (blue), MMP2, MMP3, MMP7, MMP9, and MMP13 (red), and MMP3 (purple). (based on Brekken and Sage, 2001; Chlenski and Cohn, 2010)
Description SPARC is a 43kDa protein, composed of 303 aminoacids. The first 17 amino acids containing the signaling peptide sequence is removed during protein processing. SPARC protein has three structural domains: N-terminal domain (NT; aa 3-51) encoded by exons 3 and 4, follistatin-like domain (FS; 53-137 aa) encoded by exons 5 and 6, the Extra Cellular domain (EC; aa 138-286) encoded by exons 7 to 9. It has eleven collagen- and six high-affinity Ca2+ binding residues. Also, the protein presents cleavage sites for cathepsin and members of the metalloproteinases family (Brekken and Sage, 2000; figure 1).
The NT domain binds hydroxyapatite and calcium ions. The FS domain contains several internal disulfide bonds that stabilize two weakly interacting modules. The N-terminus region of the FS domain has a very twisted-hairpin structure that is linked by disulfide bonds at cysteines 1-3, and 2-4. This distribution of disulfide bonds makes the FS-domain structurally homologous to epidermal growth factor (EGF)-like domain of factor IX, a coagulation factor. At the other end of the FS-domain, its C-terminus region has structural similarity to Kazal family of serine proteases. It has antiparallel alpha-helices connected to small three-stranded antiparallel alpha-sheets with disulfide bonds linking cysteines 5-9, 6-8, 7-10. The EC domain contains two E-F hand motifs that bind calcium with high affinity, and comprise almost entirely of alpha-helices (Hohenester et al., 1997).
Expression The evaluation of the expression pattern of SPARC protein and mRNA during human embryonic and fetal development revealed that it is usually expressed in tissues undergoing rapid proliferation (Mundlos et al., 1992). These authors also showed that earlier developmental stages showed a more general distribution, changing to more heterogeneity expression pattern in later stages. SPARC expression was observed in bone, cartilage, teeth, kidney, gonads, adrenal gland, lung, eye, vessels (Mundlos et al., 1992). In adults SPARC is expressed in different tissues and organs, including bone marrow, whole blood, lymph node, thymus, brain, cerebellum, retina, heart, smooth muscle, skeletal muscle, spinal cord, intestine, colon, adipocyte, kidney, liver, pancreas, thyroid, salivary gland, skin, ovary, uterus, placenta, cervix and prostate (Wang et al, 2014). It is also described that SPARC is expressed by different cell types including active osteoblasts, bone marrow progenitor cells, odontoblasts endothelial cells, fibroblasts, pericytes, astrocytes and macrophages (McCurdy et al. 2010; Rosseta and Bradshaw, 2016).
In cancer, according to PrognoScan database, SPARC expression was observed in bladder, blood, brain, breast, colorectal, eye tumors, glioma, head and neck cancer, lung, esophagus, ovarian, and skin cancer tissues (Wang et al., 2014).
There are evidences that SPARC expression is transcriptionally regulated by methylation in different types of neoplasia such as ovarian cancer (Socha et al, 2009), pancreatic cancer (Vaz et al, 2015), hepatocellular carcinoma (Zhang et al, 2012) colorectal (Cheetham et al., 2008) and breast (Matteucci et al, 2016). Loss of heterozygosity (LOH) at 5q has been demonstrated in pancreatic cancer (Hahn et al., 1995), in pulmonary fibrosis (Demopoulos et al., 2002) and myelodysplastic syndromes (Giagounidis et al., 2014).
Localisation SPARC is a secreted glycoprotein found mainly in the extracellular compartment. However, it has also been described to be localized both to cell nucleus and to cytoplasm (Hudson et al., 2005; Baldini et al. 2008).
Function SPARC is an evolutionarily conserved matricellular glycoprotein that is involved in diverse biological processes, including tissue remodeling, wound repair, morphogenesis, cell differentiation, proliferation, migration, and angiogenesis. Matricellular glycoprotein proteins are a family of proteins that can be associated with structural elements and mediates cell-matrix interaction rather than functions as extracellular matrix (ECM) structural elements (Bornstein, 1995).
SPARC was first described in skeletal tissue as a bone-specific protein that binds selectively to both hydroxyapatite and collagen (Termine et al., 1981). Posteriorly, it was shown that this protein is broadly expressed both in mineralized and non-mineralized tissues being associated to ECM, regulating cell-matrix interactions and cellular functions, than contributing to ECM organization (Bornstein, 2000; Bradshaw, 2012).
SPARC has also been shown to regulate the activity of matrix metalloproteinases (MMP), a family of enzymes capable of breaking down proteins, such as collagen, normally found in ECM and considered to be the mediators of ECM proteolysis and turnover. Angiogenesis, healing and metastasis, processes that require ECM restructuring are associated with higher SPARC production.
The influence of SPARC on MMP-1, MMP-3, and MMP-9 activity was first described by Tremble et al., 1993. Further studies were carried out in transformed cells and tumor, and it was demonstrated that SPARC could increase MMP-2 activity in glioma cells and breast cancer cells but not in melanoma cells (McClung et al., 2007, Nischt et al., 2001, Gilles et al., 1998).
Homology The human SPARC gene shows 92% and 31% identity with the mouse and nematode homologs, respectively.
SPARC is conserved in a wide variety of evolutionarily diverse organisms (e.g., C. elegans, Drosophila, brine shrimp, trout, chicken, mice, and humans), suggesting that it plays an important function in multicellular biology (Bradshaw and Sage, 2001).

Implicated in

Entity Osteogenesis Imperfecta, Type XVII
Note SPARC gene mutations have been correlated with a severe disease known as osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Whole-exome sequencing was carried out in 2 unrelated girls carrying osteogenesis imperfecta (OI17; 616507) leading to the identification of two different homozygous missense mutations, R166H (VAR_075142) and E263K (VAR_075143) (Mendoza-Londono et al., 2015). Previous studies described diminished expression of SPARC in osteoblasts from patients with osteogenesis imperfecta (Muriel et al., 1991) and in osteoblasts obtained from the fro/fro mouse, an animal with fragile bones (Vetter et al., 1991)
Entity Osteoporosis
Note The involvement of SPARC in bone remodeling has been described, and its expression is observed in osteoblasts express (Kelm et al., 1992). SPARC-null mice were reported to have decreased numbers of osteoblasts and osteoclasts, indicating decreased bone turnover, resulting in low turnover osteoporosis-like phenotype affecting trabecular bone (Delany, et al., 2000). Also, in osteonectin-null mice, osteonectin levels have been shown to play a role in modulating the balance of bone formation and resorption in response to PTH treatment (Machado do Reis et al., 2008).
Entity Pulmonary and Cardiac Fibrosis
Note Fibrosis is characterized by excessive deposition of extracellular matrix, resulting in tissue remodeling and thus interfering with normal tissue architecture and function.
SPARC expression and up-regulation have been reported in multiple types of fibrosis both human and animal fibrotic models. It has been shown that SPARC can influence TGFB1 (TGF-beta), a known regulator of fibrosis. Thus it is suggested that SPARC may regulate TGF-beta activity in fibrotic tissues (Trombetta and Bradshaw, 2012).
Entity Cardiac disease
Note SPARC expression was reported in cardiac disease. It is highly expressed in fibroblasts and endothelial cells and less expressed in cardiac myocytes. By screening analysis, SPARC was found as differentially expressed and potentially associated with myocardial infarction and transverse aortic constriction (Wang et al., 2015). Also, SPARC demonstrated to have potential therapeutic applications in inhibiting cardiac dilatation and dysfunction after myocardial infarction (Schelling et al., 2009).
Entity Cancer
Note SPARC protein modulates different cell functions like as adhesion, proliferation, angiogenesis, cell survival, and has been associated with tumor development and progression (Arnold and Brekken, 2009; Nagaraju et al., 2014).
SPARC is differentially expressed in different types of cancer, and its ability to inhibit or promote tumor progression is dependent on the cellular type, tumoral stage and the type of established interactions among the different components of cellular microenvironment (Arnold and Brekken, 2009).
The pleiotropic effects of SPARC reflect the complexity of actions of this protein, which can act as an oncogene or tumor suppressor (Podhajcer et al.,2008; Arnold and Brekken, 2009). Hence, the role of SPARC in the process of tumorigenesis and as a tumor biomarker is still controversial. Higher levels of SPARC were observed in malignant tumors, including breast, esophagus, brain, prostate, glioma, and melanoma, suggesting that increased SPARC expression is associated with tumor progression (Framson and Sage, 2004; Bos et al.,2004; Watkins et al.,2005; Koblinski et al.,2005). On the other hands, other studies have suggested that SPARC may act as a tumor suppressor, promoting apoptosis in ovarian cancer cells and presenting an anti-tumoral effect in pancreatic and breast cancers (Chlenski and Cohn, 2010; Nagai et al.,2011).
Tumor with high metastatic potentials such as glioblastomas, melanoma, breast and prostate cancer, express higher levels of SPARC while less metastatic tumor-like ovarian, pancreatic and colorectal tumors, expresses lower or undetectable SPARC (Feng and Tang, 2014).
The diversity of SPARC expression effects has been observed in different types of cancers. SPARC has been associated with tumor development in melanoma, esophagus cancer, gastric cancer and glioma, and data suggests that higher expression is correlated with a more aggressive phenotype such as tumor size, metastasis and poor prognosis (Yamashita K et al., 2003; Bos et al.,2004; Framson and Sage, 2004; Wang  et al., 2004; Koblinsk et al.,2005; Zhao et al., 2010; Fenouille et al., 2011; Liu et al., 2011; Rocco et al., 2011; McClung et al., 2012; Kim et al., 2013) The expression of SPARC does not seem to directly influence cellular transformation since SPARC knockout mice do not develop tumors. However, SPARC might significantly influence tumor-stroma interactions contributing to tumor progression and therapy response (Said et al., 2013).
In different tumor types such as prostatic carcinoma, neuroblastoma, pulmonary carcinoma, leukemia, pancreatic and colorectal cancer, it was described that SPARC inhibits tumor growth and reverts drug resistance increasing chemotherapy response. (Brekken et al., 2003; Sato et al., 2003; Puolakkainen et al., 2004; Said and Motamed, 2005; Tai et al., 2005; DiMartino et al., 2006; Tai and Tang, 2007; Cheetam et al., 2008; Pan et al., 2008; Wong et al., 2008; Socha et al., 2009; Bhoopathi et al., 2011; Davids and Steensma, 2010; Chew et al., 2011; Rahman et al., 2011).
Cheetham et al., 2008, showed that the demethylating agent 5-Aza-2'deoxycytidine (5-Aza) leads to the expression of SPARC and increased chemosensitivity in colon cancer cells. In irinotecan-resistant cancer cells, endogenous or exogenous SPARC exposure triggers senescence associated with increased levels of p16 and TP53 phosphorylation (Chan et al., 2010). Also, in vitro and in vivo studies have demonstrated that over-expression of the NT-domain of SPARC leads to a significantly greater sensitivity to chemotherapy and tumor regression that involves an interplay between the NT-domain, BCL2 and CASP8 (caspase 8), which increases apoptosis and confers greater chemosensitivity (Rahman et al., 2011). More recently, Fan et al., demonstrated that over-expression of SPARC increased gemcitabine-induced apoptosis in pancreatic cancer cells via up-regulation of the expression of apoptosis-related proteins. These findings provide insight on the role played by SPARC in drug sensitivity and that its re-expression has a potential to restore chemosensitivity.
Entity Breast cancer
Note In breast cancer, SPARC is expressed in more invasive but not in non-invasive cell lines (Giles et al., 1998). In normal mammary tissue, SPARC expression was undetectable or slightly detectable and in benign mammary lesions the expression was weakly positive. However, the stromal cell of 75% of in situ and invasive breast cancer samples was strongly positive for SPARC (Bellahcène and Castronovo, 1995; Barth et al., 2005; Matteucci et al., 2016).
As previous described, the role of SPARC in breast cancer is also controversial. SPARC expression is not detected in MCF-7 breast cancer cell line, however, in response to c-Jun overexpression, SPARC expression is highly induced being associated to increased invasive and migration potential (Briggs et al., 2002). Instead, in the tumorigenic model of breast cancer cells, MDA-MD-231, SPARC expression inhibited invasion and metastasis (Koblinski et al., 2005).
In breast tumors increased SPARC expression was associated with tumor progression and aggressiveness phenotype (Watkins et al., 2005 and Helleman et al. 2008). On the other hand, the reduction of SPARC protein expression was associated with poor prognosis of breast cancer patients (Hsiao et al., 2010 e Nagai et al., 2011). In breast cancer brain metastasis SPARC expression was down-regulated in comparison to primary tumors, apart from the tumoral subtype. However, among the primary tumors evaluated, triple negative subtype expressed the higher protein level (Wikman et al., 2014). Previous data of our group, have already shown that association between SPARC and triple negative tumors, and positivity to SPARC was a marker of good prognosis in comparison to those patients with reduced SPARC level (Nagai et al., 2011).


SPARC: a matricellular regulator of tumorigenesis
Arnold SA, Brekken RA
J Cell Commun Signal 2009 Dec;3(3-4):255-73
PMID 19809893
Sparc localizes to the blebs of hobit cells and human primary osteoblasts
Baldini G, Ponti C, Bortul R, Narducci P, Grill V, Martelli AM
J Cell Biochem 2008 Aug 15;104(6):2310-23
PMID 18442048
Stromal remodeling and SPARC (secreted protein acid rich in cysteine) expression in invasive ductal carcinomas of the breast
Barth PJ, Moll R, Ramaswamy A
Virchows Arch 2005 May;446(5):532-6
PMID 15838642
Increased expression of osteonectin and osteopontin, two bone matrix proteins, in human breast cancer
Bellahcène A, Castronovo V
Am J Pathol 1995 Jan;146(1):95-100
PMID 7856741
SPARC stimulates neuronal differentiation of medulloblastoma cells via the Notch1/STAT3 pathway
Bhoopathi P, Chetty C, Dontula R, Gujrati M, Dinh DH, Rao JS, Lakka SS
Cancer Res 2011 Jul 15;71(14):4908-19
PMID 21613407
Matricellular proteins: an overview
Bornstein P
J Cell Commun Signal 2009 Dec;3(3-4):163-5
PMID 19779848
International Hermelin brain tumor symposium on matricellular proteins in normal and cancer cell-matrix interactions
Bos TJ, Cohn SL, Kleinman HK, Murphy-Ulrich JE, Podhajcer OL, Rempel SA, Rich JN, Rutka JT, Sage EH, Thompson EW
Matrix Biol 2004 Apr;23(1):63-9
PMID 15230275
Diverse biological functions of the SPARC family of proteins
Bradshaw AD
Int J Biochem Cell Biol 2012 Mar;44(3):480-8
PMID 22249026
SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury
Bradshaw AD, Sage EH
J Clin Invest 2001 May;107(9):1049-54
PMID 11342565
SPARC, a matricellular protein: at the crossroads of cell-matrix communication
Brekken RA, Sage EH
Matrix Biol 2001 Jan;19(8):816-27
PMID 11223341
Transcriptional upregulation of SPARC, in response to c-Jun overexpression, contributes to increased motility and invasion of MCF7 breast cancer cells
Briggs J, Chamboredon S, Castellazzi M, Kerry JA, Bos TJ
Oncogene 2002 Oct 10;21(46):7077-91
PMID 12370830
Secreted protein acidic and rich in cysteine-induced cellular senescence in colorectal cancers in response to irinotecan is mediated by P53
Chan JM, Ho SH, Tai IT
Carcinogenesis 2010 May;31(5):812-9
PMID 20164124
SPARC promoter hypermethylation in colorectal cancers can be reversed by 5-Aza-2'deoxycytidine to increase SPARC expression and improve therapy response
Cheetham S, Tang MJ, Mesak F, Kennecke H, Owen D, Tai IT
Br J Cancer 2008 Jun 3;98(11):1810-9
PMID 18458674
SPARC, FOXP3, CD8 and CD45 correlation with disease recurrence and long-term disease-free survival in colorectal cancer
Chew A, Salama P, Robbshaw A, Klopcic B, Zeps N, Platell C, Lawrance IC
PLoS One 2011;6(7):e22047
PMID 21818290
Modulation of matrix remodeling by SPARC in neoplastic progression
Chlenski A, Cohn SL
Semin Cell Dev Biol 2010 Feb;21(1):55-65
PMID 19958839
The molecular pathogenesis of myelodysplastic syndromes
Davids MS, Steensma DP
Cancer Biol Ther 2010 Aug 15;10(4):309-19
PMID 20592488
Osteopenia and decreased bone formation in osteonectin-deficient mice
Delany AM, Amling M, Priemel M, Howe C, Baron R, Canalis E
J Clin Invest 2000 Apr;105(7):915-23
PMID 10749571
MYCL1, FHIT, SPARC, p16(INK4) and TP53 genes associated to lung cancer in idiopathic pulmonary fibrosis
Demopoulos K, Arvanitis DA, Vassilakis DA, Siafakas NM, Spandidos DA
J Cell Mol Med 2002 Apr-Jun;6(2):215-22
PMID 12169206
Low or absent SPARC expression in acute myeloid leukemia with MLL rearrangements is associated with sensitivity to growth inhibition by exogenous SPARC protein
DiMartino JF, Lacayo NJ, Varadi M, Li L, Saraiya C, Ravindranath Y, Yu R, Sikic BI, Raimondi SC, Dahl GV
Leukemia 2006 Mar;20(3):426-32
PMID 16424866
Secreted protein acidic and rich in cysteine enhances the chemosensitivity of pancreatic cancer cells to gemcitabine
Fan X, Mao Z, Ma X, Cui L, Qu J, Lv L, Dang S, Wang X, Zhang J
Tumour Biol 2016 Feb;37(2):2267-73
PMID 26358255
SPARC in Tumor Pathophysiology and as a Potential Therapeutic Target
Feng J, Tang L
Curr Pharm Des 2014;20(39):6182-90
PMID 24947586
SPARC functions as an anti-stress factor by inactivating p53 through Akt-mediated MDM2 phosphorylation to promote melanoma cell survival
Fenouille N, Puissant A, Tichet M, Zimniak G, Abbe P, Mallavialle A, Rocchi S, Ortonne JP, Deckert M, Ballotti R, Tartare-Deckert S
Oncogene 2011 Dec 8;30(49):4887-900
PMID 21685937
SPARC and tumor growth: where the seed meets the soil? J Cell Biochem
Framson PE, Sage EH
2004 Jul 1;92(4):679-90 Review
PMID 15211566
Lenalidomide as a disease-modifying agent in patients with del(5q) myelodysplastic syndromes: linking mechanism of action to clinical outcomes
Giagounidis A, Mufti GJ, Fenaux P, Germing U, List A, MacBeth KJ
Ann Hematol 2014 Jan;93(1):1-11
PMID 24018623
SPARC/osteonectin induces matrix metalloproteinase 2 activation in human breast cancer cell lines
Gilles C, Bassuk JA, Pulyaeva H, Sage EH, Foidart JM, Thompson EW
Cancer Res 1998 Dec 1;58(23):5529-36
PMID 9850090
Allelotype of pancreatic adenocarcinoma using xenograft enrichment
Hahn SA, Seymour AB, Hoque AT, Schutte M, da Costa LT, Redston MS, Caldas C, Weinstein CL, Fischer A, Yeo CJ, et al
Cancer Res 1995 Oct 15;55(20):4670-5
PMID 7553647
Association of an extracellular matrix gene cluster with breast cancer prognosis and endocrine therapy response
Helleman J, Jansen MP, Ruigrok-Ritstier K, van Staveren IL, Look MP, Meijer-van Gelder ME, Sieuwerts AM, Klijn JG, Sleijfer S, Foekens JA, Berns EM
Clin Cancer Res 2008 Sep 1;14(17):5555-64
PMID 18765548
Crystal structure of a pair of follistatin-like and EF-hand calcium-binding domains in BM-40
Hohenester E, Maurer P, Timpl R
EMBO J 1997 Jul 1;16(13):3778-86
PMID 9233787
SPARC (osteonectin) in breast tumors of different histologic types and its role in the outcome of invasive ductal carcinoma
Hsiao YH, Lien HC, Hwa HL, Kuo WH, Chang KJ, Hsieh FJ
Breast J 2010 May-Jun;16(3):305-8
PMID 20210803
Spreading of embryologically distinct urothelial cells is inhibited by SPARC
Hudson AE, Feng WC, Delostrinos CF, Carmean N, Bassuk JA
J Cell Physiol 2005 Feb;202(2):453-63
PMID 15389586
Characterization of human osteoblast and megakaryocyte-derived osteonectin (SPARC)
Kelm RJ Jr, Hair GA, Mann KG, Grant BW
Blood 1992 Dec 15;80(12):3112-9
PMID 1467517
Expression of Secreted Protein Acidic and Rich in Cysteine in the Stroma of a Colorectal Carcinoma is Associated With Patient Prognosis
Kim JY, Jeong D, Ahn TS, Kim HJ, Park DS, Park SY, Bae SB, Lee S, Lee SS, Lee MS, Cho HD, Baek MJ
Ann Coloproctol 2013 Jun;29(3):93-9
PMID 23862126
Endogenous osteonectin/SPARC/BM-40 expression inhibits MDA-MB-231 breast cancer cell metastasis
Koblinski JE, Kaplan-Singer BR, VanOsdol SJ, Wu M, Engbring JA, Wang S, Goldsmith CM, Piper JT, Vostal JG, Harms JF, Welch DR, Kleinman HK
Cancer Res 2005 Aug 15;65(16):7370-7
PMID 16103089
Knockdown of secreted protein acidic and rich in cysteine (SPARC) expression diminishes radiosensitivity of glioma cells
Liu H, Zhang H, Jiang X, Ma Y, Xu Y, Feng S, Liu F
Cancer Biother Radiopharm 2011 Dec;26(6):705-15
PMID 22145659
Accentuated osteoclastic response to parathyroid hormone undermines bone mass acquisition in osteonectin-null mice
Machado do Reis L, Kessler CB, Adams DJ, Lorenzo J, Jorgetti V, Delany AM
Bone 2008 Aug;43(2):264-73
PMID 18499553
Coordinate regulation of microenvironmental stimuli and role of methylation in bone metastasis from breast carcinoma
Matteucci E, Maroni P, Disanza A, Bendinelli P, Desiderio MA
Biochim Biophys Acta 2016 Jan;1863(1):64-76
PMID 26481505
Deletion of the SPARC acidic domain or EGF-like module reduces SPARC-induced migration and signaling through p38 MAPK/HSP27 in glioma
McClung HM, Golembieski WA, Schultz CR, Jankowski M, Schultz LR, Rempel SA
Carcinogenesis 2012 Feb;33(2):275-84
PMID 22114076
Cardiac extracellular matrix remodeling: fibrillar collagens and Secreted Protein Acidic and Rich in Cysteine (SPARC)
McCurdy S, Baicu CF, Heymans S, Bradshaw AD
J Mol Cell Cardiol 2010 Mar;48(3):544-9
PMID 19577572
, Tétreault M, Nadaf J, Kannu P, Sochett E, Howard A, Stimec J, Dupuis L, Roschger P, Klaushofer K, Palomo T, Ouellet J, Al-Jallad H, Mort JS, Moffatt P, Boudko S, Bächinger HP, Rauch F
Mendoza-Londono R, Fahiminiya S, Majewski J; Care4Rare Canada Consortium
Recessive osteogenesis imperfecta caused by missense mutations in SPARC Am J Hum Genet
PMID 26027498
Distribution of osteonectin mRNA and protein during human embryonic and fetal development
Mundlos S, Schwahn B, Reichert T, Zabel B
J Histochem Cytochem 1992 Feb;40(2):283-91
PMID 1552170
Morphological and biochemical studies of a mouse mutant (fro/fro) with bone fragility
Muriel MP, Bonaventure J, Stanescu R, Maroteaux P, Guénet JL, Stanescu V
Bone 1991;12(4):241-8
PMID 1793673
Prognostic value of NDRG1 and SPARC protein expression in breast cancer patients
Nagai MA, Gerhard R, Fregnani JH, Nonogaki S, Rierger RB, Netto MM, Soares FA
Breast Cancer Res Treat 2011 Feb;126(1):1-14
PMID 20369286
Molecular mechanisms underlying the divergent roles of SPARC in human carcinogenesis
Nagaraju GP, Dontula R, El-Rayes BF, Lakka SS
Carcinogenesis 2014 May;35(5):967-73
PMID 24675529
BM-40 and MMP-2 expression are not coregulated in human melanoma cell lines
Nischt R, Wallich M, Reibetanz M, Baumann P, Krieg T, Mauch C
Cancer Lett 2001 Jan 26;162(2):223-30
PMID 11146229
The nonsteroidal anti-inflammatory drug NS398 reactivates SPARC expression via promoter demethylation to attenuate invasiveness of lung cancer cells
Pan MR, Chang HC, Chuang LY, Hung WC
Exp Biol Med (Maywood) 2008 Apr;233(4):456-62
PMID 18367635
The role of the matricellular protein SPARC in the dynamic interaction between the tumor and the host
Podhajcer OL, Benedetti LG, Girotti MR, Prada F, Salvatierra E, Llera AS
Cancer Metastasis Rev 2008 Dec;27(4):691-705
PMID 18542844
Enhanced growth of pancreatic tumors in SPARC-null mice is associated with decreased deposition of extracellular matrix and reduced tumor cell apoptosis
Puolakkainen PA, Brekken RA, Muneer S, Sage EH
Mol Cancer Res 2004 Apr;2(4):215-24
PMID 15140943
A peptide of SPARC interferes with the interaction between caspase8 and Bcl2 to resensitize chemoresistant tumors and enhance their regression in vivo
Rahman M, Chan AP, Tang M, Tai IT
PLoS One 2011;6(11):e26390
PMID 22069448
Proteomic profiling of human melanoma metastatic cell line secretomes
Rocco M, Malorni L, Cozzolino R, Palmieri G, Rozzo C, Manca A, Parente A, Chambery A
J Proteome Res 2011 Oct 7;10(10):4703-14
PMID 21815687
SPARC/osteonectin in mineralized tissue
Rosset EM, Bradshaw AD
Matrix Biol 2016 May-Jul;52-54:78-87
PMID 26851678
Characterization of a novel serum albumin-binding glycoprotein secreted by endothelial cells in culture
Sage H, Johnson C, Bornstein P
J Biol Chem 1984 Mar 25;259(6):3993-4007
PMID 6368555
Loss of SPARC in bladder cancer enhances carcinogenesis and progression
Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D
J Clin Invest 2013 Feb;123(2):751-66
PMID 23321672
Absence of host-secreted protein acidic and rich in cysteine (SPARC) augments peritoneal ovarian carcinomatosis
Said N, Motamed K
Am J Pathol 2005 Dec;167(6):1739-52
PMID 16314484
SPARC/osteonectin is a frequent target for aberrant methylation in pancreatic adenocarcinoma and a mediator of tumor-stromal interactions
Sato N, Fukushima N, Maehara N, Matsubayashi H, Koopmann J, Su GH, Hruban RH, Goggins M
Oncogene 2003 Aug 7;22(32):5021-30
PMID 12902985
Absence of SPARC results in increased cardiac rupture and dysfunction after acute myocardial infarction
Schellings MW, Vanhoutte D, Swinnen M, Cleutjens JP, Debets J, van Leeuwen RE, d'Hooge J, Van de Werf F, Carmeliet P, Pinto YM, Sage EH, Heymans S
J Exp Med 2009 Jan 16;206(1):113-23
PMID 19103879
Aberrant promoter methylation of SPARC in ovarian cancer
Socha MJ, Said N, Dai Y, Kwong J, Ramalingam P, Trieu V, Desai N, Mok SC, Motamed K
Neoplasia 2009 Feb;11(2):126-35
PMID 19177197
Genome-wide expression analysis of therapy-resistant tumors reveals SPARC as a novel target for cancer therapy
Tai IT, Dai M, Owen DA, Chen LB
J Clin Invest 2005 Jun;115(6):1492-502
PMID 15902309
A novel interaction between procaspase 8 and SPARC enhances apoptosis and potentiates chemotherapy sensitivity in colorectal cancers
Tang MJ, Tai IT
J Biol Chem 2007 Nov 23;282(47):34457-67
PMID 17897953
Osteonectin, a bone-specific protein linking mineral to collagen
Termine JD, Kleinman HK, Whitson SW, Conn KM, McGarvey ML, Martin GR
Cell 1981 Oct;26(1 Pt 1):99-105
PMID 7034958
SPARC, a secreted protein associated with morphogenesis and tissue remodeling, induces expression of metalloproteinases in fibroblasts through a novel extracellular matrix-dependent pathway
Tremble PM, Lane TF, Sage EH, Werb Z
J Cell Biol 1993 Jun;121(6):1433-44
PMID 8509459
The Function of SPARC as a Mediator of Fibrosis
Trombetta-Esilva J, Bradshaw AD
Open Rheumatol J 2012;6:146-55
PMID 22802913
Osteogenesis imperfecta: changes in noncollagenous proteins in bone
Vetter U, Fisher LW, Mintz KP, Kopp JB, Tuross N, Termine JD, Robey PG
J Bone Miner Res 1991 May;6(5):501-5
PMID 2068957
Integrative genomic analyses of secreted protein acidic and rich in cysteine and its role in cancer prediction
Wang B, Chen K, Xu W, Chen D, Tang W, Xia TS
Mol Med Rep 2014 Sep;10(3):1461-8
PMID 24938427
Overexpression of SPARC gene in human gastric carcinoma and its clinic-pathologic significance
Wang CS, Lin KH, Chen SL, Chan YF, Hsueh S
Br J Cancer 2004 Nov 29;91(11):1924-30
PMID 15558074
Association of serum SPARC level with severity of coronary artery lesion in type 2 diabetic patients with coronary heart disease
Wang Z, Song HY, An MM, Zhu LL
Int J Clin Exp Med 2015 Oct 15;8(10):19290-6
PMID 26770566
Increased levels of SPARC (osteonectin) in human breast cancer tissues and its association with clinical outcomes
Watkins G, Douglas-Jones A, Bryce R, Mansel RE, Jiang WG
Prostaglandins Leukot Essent Fatty Acids 2005 Apr;72(4):267-72
PMID 15763438
Loss of CADM1 expression is associated with poor prognosis and brain metastasis in breast cancer patients
Wikman H, Westphal L, Schmid F, Pollari S, Kropidlowski J, Sielaff-Frimpong B, Glatzel M, Matschke J, Westphal M, Iljin K, Huhtala H, Terracciano L, Kallioniemi A, Sauter G, Müller V, Witzel I, Lamszus K, Kemming D, Pantel K
Oncotarget 2014 May 30;5(10):3076-87
PMID 24833255
Analyses of the role of endogenous SPARC in mouse models of prostate and breast cancer
Wong SY, Crowley D, Bronson RT, Hynes RO
Clin Exp Metastasis 2008;25(2):109-18
PMID 18058030
Clinical significance of secreted protein acidic and rich in cystein in esophageal carcinoma and its relation to carcinoma progression
Yamashita K, Upadhay S, Mimori K, Inoue H, Mori M
Cancer 2003 May 15;97(10):2412-9
PMID 12733139
Frequent inactivation of SPARC by promoter hypermethylation in colon cancers
Yang E, Kang HJ, Koh KH, Rhee H, Kim NK, Kim H
Int J Cancer 2007 Aug 1;121(3):567-75
PMID 17397030
Aberrant methylation of SPARC in human hepatocellular carcinoma and its clinical implication
Zhang Y, Yang B, Du Z, Bai T, Gao YT, Wang YJ, Lou C, Wang FM, Bai Y
World J Gastroenterol 2012 May 7;18(17):2043-52
PMID 22563191
SPARC is associated with gastric cancer progression and poor survival of patients
Zhao ZS, Wang YY, Chu YQ, Ye ZY, Tao HQ
Clin Cancer Res 2010 Jan 1;16(1):260-8
PMID 20028745


This paper should be referenced as such :
Ana C Pavanelli, Flavia Regina Mangone, Maria A Nagai
SPARC (secreted protein acidic and cysteine-rich)
Atlas Genet Cytogenet Oncol Haematol. 2017;21(10):351-357.
Free journal version : [ pdf ]   [ DOI ]

Other Leukemias implicated (Data extracted from papers in the Atlas) [ 1 ]
  Classification of myelodysplastic syndromes 2015

External links


HGNC (Hugo)SPARC   11219
Entrez_Gene (NCBI)SPARC    secreted protein acidic and cysteine rich
AliasesBM-40; OI17; ON; ONT
GeneCards (Weizmann)SPARC
Ensembl hg19 (Hinxton)ENSG00000113140 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000113140 [Gene_View]  ENSG00000113140 [Sequence]  chr5:151661096-151686915 [Contig_View]  SPARC [Vega]
ICGC DataPortalENSG00000113140
Genatlas (Paris)SPARC
Genetics Home Reference (NIH)SPARC
Genomic and cartography
GoldenPath hg38 (UCSC)SPARC  -     chr5:151661096-151686915 -  5q33.1   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)SPARC  -     5q33.1   [Description]    (hg19-Feb_2009)
GoldenPathSPARC - 5q33.1 [CytoView hg19]  SPARC - 5q33.1 [CytoView hg38]
Genome Data Viewer NCBISPARC [Mapview hg19]  
OMIM182120   616507   
Gene and transcription
Genbank (Entrez)AK096969 AK299441 AK315591 AL540785 AL541830
RefSeq transcript (Entrez)NM_001309443 NM_001309444 NM_003118
Consensus coding sequences : CCDS (NCBI)SPARC
Gene Expression Viewer (FireBrowse)SPARC [ Firebrowse - Broad ]
GenevisibleExpression of SPARC in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)6678
GTEX Portal (Tissue expression)SPARC
Human Protein AtlasENSG00000113140-SPARC [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtP09486   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtP09486  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProP09486
Domaine pattern : Prosite (Expaxy)EF_HAND_1 (PS00018)    KAZAL_2 (PS51465)    OSTEONECTIN_1 (PS00612)    OSTEONECTIN_2 (PS00613)   
Domains : Interpro (EBI)EF-hand-dom_pair    EF_Hand_1_Ca_BS    Fol_N    Follistatin/Osteonectin_EGF    Kazal_dom    Kazal_dom_sf    Osteonectin_CS    SPARC    SPARC/Testican_Ca-bd-dom   
Domain families : Pfam (Sanger)FOLN (PF09289)    Kazal_1 (PF00050)    SPARC_Ca_bdg (PF10591)   
Domain families : Pfam (NCBI)pfam09289    pfam00050    pfam10591   
Domain families : Smart (EMBL)FOLN (SM00274)  KAZAL (SM00280)  
Conserved Domain (NCBI)SPARC
PDB (RSDB)1BMO    1NUB    1SRA    2V53   
PDB Europe1BMO    1NUB    1SRA    2V53   
PDB (PDBSum)1BMO    1NUB    1SRA    2V53   
PDB (IMB)1BMO    1NUB    1SRA    2V53   
Structural Biology KnowledgeBase1BMO    1NUB    1SRA    2V53   
SCOP (Structural Classification of Proteins)1BMO    1NUB    1SRA    2V53   
CATH (Classification of proteins structures)1BMO    1NUB    1SRA    2V53   
AlphaFold pdb e-kbP09486   
Human Protein Atlas [tissue]ENSG00000113140-SPARC [tissue]
Protein Interaction databases
IntAct (EBI)P09486
Ontologies - Pathways
Ontology : AmiGOossification  negative regulation of endothelial cell proliferation  platelet degranulation  extracellular matrix structural constituent  calcium ion binding  calcium ion binding  protein binding  collagen binding  collagen binding  extracellular region  extracellular region  extracellular region  basement membrane  extracellular space  cytoplasm  cytoplasm  mitochondrion  plasma membrane  plasma membrane  receptor-mediated endocytosis  heart development  response to gravity  cell surface  response to lead ion  positive regulation of endothelial cell migration  nuclear matrix  negative regulation of angiogenesis  regulation of cell morphogenesis  extracellular matrix organization  lung development  platelet alpha granule  platelet alpha granule membrane  platelet alpha granule lumen  response to lipopolysaccharide  response to L-ascorbic acid  response to cytokine  wound healing  intracellular membrane-bounded organelle  response to peptide hormone  response to ethanol  response to cadmium ion  inner ear development  anatomical structure development  regulation of synapse organization  extracellular matrix binding  response to glucocorticoid  response to cAMP  response to calcium ion  collagen-containing extracellular matrix  endocytic vesicle lumen  glutamatergic synapse  
Ontology : EGO-EBIossification  negative regulation of endothelial cell proliferation  platelet degranulation  extracellular matrix structural constituent  calcium ion binding  calcium ion binding  protein binding  collagen binding  collagen binding  extracellular region  extracellular region  extracellular region  basement membrane  extracellular space  cytoplasm  cytoplasm  mitochondrion  plasma membrane  plasma membrane  receptor-mediated endocytosis  heart development  response to gravity  cell surface  response to lead ion  positive regulation of endothelial cell migration  nuclear matrix  negative regulation of angiogenesis  regulation of cell morphogenesis  extracellular matrix organization  lung development  platelet alpha granule  platelet alpha granule membrane  platelet alpha granule lumen  response to lipopolysaccharide  response to L-ascorbic acid  response to cytokine  wound healing  intracellular membrane-bounded organelle  response to peptide hormone  response to ethanol  response to cadmium ion  inner ear development  anatomical structure development  regulation of synapse organization  extracellular matrix binding  response to glucocorticoid  response to cAMP  response to calcium ion  collagen-containing extracellular matrix  endocytic vesicle lumen  glutamatergic synapse  
REACTOMEP09486 [protein]
REACTOME PathwaysR-HSA-3000497 [pathway]   
Atlas of Cancer Signalling NetworkSPARC
Wikipedia pathwaysSPARC
Orthology - Evolution
GeneTree (enSembl)ENSG00000113140
Phylogenetic Trees/Animal Genes : TreeFamSPARC
Homologs : HomoloGeneSPARC
Homology/Alignments : Family Browser (UCSC)SPARC
Gene fusions - Rearrangements
Fusion : QuiverSPARC
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerSPARC [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)SPARC
Exome Variant ServerSPARC
GNOMAD BrowserENSG00000113140
Varsome BrowserSPARC
ACMGSPARC variants
Genomic Variants (DGV)SPARC [DGVbeta]
DECIPHERSPARC [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisSPARC 
ICGC Data PortalSPARC 
TCGA Data PortalSPARC 
Broad Tumor PortalSPARC
OASIS PortalSPARC [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICSPARC  [overview]  [genome browser]  [tissue]  [distribution]  
Somatic Mutations in Cancer : COSMIC3DSPARC
Mutations and Diseases : HGMDSPARC
LOVD (Leiden Open Variation Database)[gene] [transcripts] [variants]
DgiDB (Drug Gene Interaction Database)SPARC
DoCM (Curated mutations)SPARC
CIViC (Clinical Interpretations of Variants in Cancer)SPARC
Impact of mutations[PolyPhen2] [Provean] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
OMIM182120    616507   
Genetic Testing Registry SPARC
NextProtP09486 [Medical]
Target ValidationSPARC
Huge Navigator SPARC [HugePedia]
Clinical trials, drugs, therapy
Protein Interactions : CTDSPARC
Pharm GKB GenePA36055
Clinical trialSPARC
DataMed IndexSPARC
PubMed329 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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indexed on : Fri Oct 8 21:28:34 CEST 2021

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