| Note | SSX2IP was discovered due to its interaction with SSX2 in a yeast two-hybrid system and believed to regulate the function of SSX2 in the testes and malignant cells (de Bruijn et al., 2002). |
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| | Structural representation of SSX2IP protein in human, mouse and chicken showing the binding regions (Breslin et al., 2007). |
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| Description | There are 3-4 coil coiled regions in each version of SSX2IP. Only human SSX2IP has a nuclear localisation signal (NLS). |
| Expression | Expression observed in various normal tissues, the highest being in the brain (de Bruijn et al., 2002). It is expressed less but significantly in kidney, testes, spinal cord, liver, heart, lung, pancreas, skeletal muscle, ovary, placenta, foetal liver and foetal brain (Breslin et al., 2007). |
| Localisation | SSX2IP co-localises with SSX2 to the nucleus and some fragments to the cytoplasm (de Bruijn et al., 2002). It has also been seen to localise on the surface of myeloid cell lines and primary AML (Denniss et al., 2007). ADIP co-localises with afadin at adherens junctions and in perinuclear regions (Asada et al., 2003). |
| Function | It has been suggested that SSX2IP regulates the function of SSX2 (de Bruijn et al., 2002). Rodent ADIP binds with F-actin binding proteins afadin and α-actinin and could therefore be involved in forming actin structure at cell-cell adherens junction as well as construction of actin bundle at nectin-based cell adhesion sites (Asada et al., 2003). Through its interaction with the actin-binding protein β-spectrin, ADIP may have a role in actin-dependent organization of the Golgi complex. ADIP binds β'-cop, which is a subunit of the coatomer complex, proposing a role in vesicle trafficking (Asada et al., 2004). |
| Afadin- and alpha-actinin-binding protein ADIP directly binds beta'-COP, a subunit of the coatomer complex. |
| Asada M, Irie K, Yamada A, Takai Y. |
| Biochem Biophys Res Commun. 2004 Aug 20;321(2):350-4. |
| PMID 15358183 |
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| SSX2IP: an emerging role in cancer. |
| Breslin A, Denniss FA, Guinn BA. |
| Biochem Biophys Res Commun. 2007 Nov 23;363(3):462-5. Epub 2007 Sep 24. (REVIEW) |
| PMID 17904521 |
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| The leukaemia-associated antigen, SSX2IP, is expressed during mitosis on the surface of myeloid leukaemia cells. |
| Denniss FA, Breslin A, Ingram W, Hardwick NR, Mufti GJ, Guinn BA. |
| Br J Haematol. 2007 Sep;138(5):668-9. |
| PMID 17686061 |
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| Elevated expression of the leukemia-associated antigen SSX2IP predicts survival in acute myeloid leukemia patients who lack detectable cytogenetic rearrangements. |
| Guinn B, Greiner J, Schmitt M, Mills KI. |
| Blood. 2009 Jan 29;113(5):1203-4. |
| PMID 19179477 |
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| Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia. |
| Guinn BA, Bland EA, Lodi U, Liggins AP, Tobal K, Petters S, Wells JW, Banham AH, Mufti GJ. |
| Biochem Biophys Res Commun. 2005 Oct 7;335(4):1293-304. |
| PMID 16112646 |
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| SSX2IP expression in acute myeloid leukaemia: an association with mitotic spindle failure in t(8;21), and cell cycle in t(15;17) patients. |
| Guinn BA, Bullinger L, Thomas NS, Mills KI, Greiner J. |
| Br J Haematol. 2008 Jan;140(2):250-1. Epub 2007 Nov 19. |
| PMID 18028484 |
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| AceView: a comprehensive cDNA-supported gene and transcripts annotation. |
| Thierry-Mieg D, Thierry-Mieg J. |
| Genome Biol. 2006;7 Suppl 1:S12.1-14. Epub 2006 Aug 7. |
| PMID 16925834 |
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| The cancer-related protein SSX2 interacts with the human homologue of a Ras-like GTPase interactor, RAB3IP, and a novel nuclear protein, SSX2IP. |
| de Bruijn DR, dos Santos NR, Kater-Baats E, Thijssen J, van den Berk L, Stap J, Balemans M, Schepens M, Merkx G, van Kessel AG. |
| Genes Chromosomes Cancer. 2002 Jul;34(3):285-98. |
| PMID 12007189 |
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