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TGFBI (transforming growth factor, beta-induced, 68kDa)

Identity

Other namesBIGH3
CDB1
CDG2
CDGG1
CSD
CSD1
CSD2
CSD3
EBMD
Kerato-epithelin
LCD1
RGD-CAP
HGNC (Hugo) TGFBI
LocusID (NCBI) 7045
Location 5q31.1
Location_base_pair Starts at 135364584 and ends at 135399507 bp from pter ( according to hg19-Feb_2009)  [Mapping]

DNA/RNA

Description 19 exons.
Transcription 2.8 Kb mRNA, 2049 bp open reading frame.

Protein

 
  TGFBI contains a secretory signal peptide (SP) at the N-terminus, followed by a cysteine-rich domain (CRD), four internal homologous domains (FAS), and a C-terminal RGD motif.
Description TGFBI is a 683 amino acid extracellular matrix protein, 68 kDa. Contains an N-terminal secretory signal peptide, a cysteine-rich domain, four internal homologous repeats (fasciclin-like FAS domains), and a C-terminal RGD motif.
Expression TGFBI is normally found in thymus, bone marrow, spleen, brain, heart, skeleton muscle, lung, kidney, liver, pancreas, and prostate.
Localisation TGFBI is an extracellular matrix protein. It localizes in the extracellular matrix.
Function Binds to type I, II, IV, VI collagens and fibronectin. The RGD motif may serve as a ligand recognition sequence for integrins. The protein may be involved in cell-matrix interactions, cell adhesion, migration and differentiation. The protein may be involved in endochondrial bone formation in cartilage. The roles of TGFBI in malignant progression are controversial. Some studies suggested that TGFBI suppresses the progression of ovarian, lung cancer and neuroblastomas, while other reports identify TGFBI as an overexpressed gene in colon, pancreatic, and liver cancer.
Homology TGFBI contains four FAS1 domains. Proteins cantaining the FAS domain include Arabidopsis fasciclin-like arabinogalactan proteins, bacterial immunogenic protein MPT70, human extracellular matrix protein periostin, and mammalian stabilin proteins.

Mutations

Germinal Mutations in the human TGFBI gene have been linked to several inherited autosmal dominant corneal dystrophies. The abnormal protein deposits in the forms of amyloid fibrils and/or non-amyloid amorphous affregations in the corneal matrix. Progressive corneal cloudiness eventually leads to severe visual loss in later stage disease. Based on the clinial histopathological properties of the deposits, corneal dystrophy can be divided into two main types: lattice corneal dystrophy (LCD) and granular corneal dystrophy (GCD). These two types of corneal dystrophies are further divided into subtypes according to the differences in the clinical features of the disease. So far, 33 mutations have been identified in the TGFBI gene associated with all the GCDs and most of the LCDs, with two major mutational sites Arg124 and Arg555, accounting for more than half of all the patients with the disease.

Implicated in

Entity Colon Cancer
Prognosis Colon cancers associated with overexpression of TGFBI may have an increased metastatic potential, leading to poor prognosis in cancer patients.
Oncogenesis Upregulation of TGFBI is associated with high-grade human colon cancers. We have found that TGFBI promotes extravasation, a critical step in the metastatic dissemination of cancer cells, by inducing the dissociation of VE-cadherin junctions between endothelial cells via activation of the integrin alphavbeta5-Src signaling pathway.
  
Entity Pancreatic Cancer
Oncogenesis TGFBI was found induced by TGFbeta1 in pancreatic cancer cell lines (CAPAN-1, PANC-1). In human pancreatic tissues, TGFBI was 32.4 fold upregulated in pancreatic cancers in comparison to normal control tissues at mRNA level.
  
Entity Ovarian Cancer
Oncogenesis Loss of TGFBI induce specific resistance to paclitaxel and mitotic spindle abnormalities in ovarian cancer cells. TGFBI expression restores the paclitaxel sensitivity via FAK- and RHO- dependent stabilization of microtubules.
  
Entity Lung Cancer
Oncogenesis TGFBI protein was absent or reduced in 45 of 130 primary lung carcinomas in comparison to normal lung tissues.
  
Entity Neuroblastoma
Oncogenesis Enhanced expression of TGFBI in human neuroblastoma cells suppresses neuroblastoma cell cohesion and adhesion to various ECM proteins. TGFBI also inhibits neuroblastoma cell proliferation and invasion.
  
Entity Liver Cancer
Oncogenesis TGFBI expression promotes cell adhesion, invasion and MMP secretion of human hepatoma cell line SMMC-7721.
  

External links

Nomenclature
HGNC (Hugo)TGFBI   11771
Cards
AtlasTGFBIID42539ch5q31
Entrez_Gene (NCBI)TGFBI  7045  transforming growth factor, beta-induced, 68kDa
GeneCards (Weizmann)TGFBI
Ensembl (Hinxton)ENSG00000120708 [Gene_View]  chr5:135364584-135399507 [Contig_View]  TGFBI [Vega]
ICGC DataPortalENSG00000120708
AceView (NCBI)TGFBI
Genatlas (Paris)TGFBI
WikiGenes7045
SOURCE (Princeton)NM_000358
Genomic and cartography
GoldenPath (UCSC)TGFBI  -  5q31.1   chr5:135364584-135399507 +  5q31   [Description]    (hg19-Feb_2009)
EnsemblTGFBI - 5q31 [CytoView]
Mapping of homologs : NCBITGFBI [Mapview]
OMIM121820   121900   122200   601692   602082   607541   608470   608471   
Gene and transcription
Genbank (Entrez)AB209598 AK093916 AK094055 AK094581 AK222833
RefSeq transcript (Entrez)NM_000358
RefSeq genomic (Entrez)AC_000137 NC_000005 NC_018916 NG_012646 NT_034772 NW_001838952 NW_004929323
Consensus coding sequences : CCDS (NCBI)TGFBI
Cluster EST : UnigeneHs.369397 [ NCBI ]
CGAP (NCI)Hs.369397
Alternative Splicing : Fast-db (Paris)GSHG0024260
Alternative Splicing GalleryENSG00000120708
Gene ExpressionTGFBI [ NCBI-GEO ]     TGFBI [ SEEK ]   TGFBI [ MEM ]
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ15582 (Uniprot)
NextProtQ15582  [Medical]
With graphics : InterProQ15582
Splice isoforms : SwissVarQ15582 (Swissvar)
Domaine pattern : Prosite (Expaxy)EMI (PS51041)    FAS1 (PS50213)   
Domains : Interpro (EBI)EMI_domain    FAS1_domain    TGFb-ind_bIGH3/osteoblast_fac2   
Related proteins : CluSTrQ15582
Domain families : Pfam (Sanger)Fasciclin (PF02469)   
Domain families : Pfam (NCBI)pfam02469   
Domain families : Smart (EMBL)FAS1 (SM00554)  
DMDM Disease mutations7045
Blocks (Seattle)Q15582
PDB (SRS)1X3B    2LTB    2LTC    2VXP   
PDB (PDBSum)1X3B    2LTB    2LTC    2VXP   
PDB (IMB)1X3B    2LTB    2LTC    2VXP   
PDB (RSDB)1X3B    2LTB    2LTC    2VXP   
Human Protein AtlasENSG00000120708
Peptide AtlasQ15582
HPRD03409
IPIIPI00018219   IPI00873923   IPI00965868   IPI00556324   IPI00940091   IPI00965607   IPI00965111   IPI00967087   IPI00966527   IPI00966279   
Protein Interaction databases
DIP (DOE-UCLA)Q15582
IntAct (EBI)Q15582
FunCoupENSG00000120708
BioGRIDTGFBI
IntegromeDBTGFBI
STRING (EMBL)TGFBI
Ontologies - Pathways
QuickGOQ15582
Ontology : AmiGOangiogenesis  chondrocyte differentiation  integrin binding  protein binding  collagen binding  extracellular region  basement membrane  extracellular space  trans-Golgi network  plasma membrane  cell adhesion  negative regulation of cell adhesion  visual perception  cell proliferation  extracellular matrix organization  extracellular matrix  extracellular matrix  extracellular matrix binding  response to stimulus  extracellular vesicular exosome  
Ontology : EGO-EBIangiogenesis  chondrocyte differentiation  integrin binding  protein binding  collagen binding  extracellular region  basement membrane  extracellular space  trans-Golgi network  plasma membrane  cell adhesion  negative regulation of cell adhesion  visual perception  cell proliferation  extracellular matrix organization  extracellular matrix  extracellular matrix  extracellular matrix binding  response to stimulus  extracellular vesicular exosome  
REACTOMEQ15582 [protein]
REACTOME PathwaysREACT_116125 Disease [pathway]
Protein Interaction DatabaseTGFBI
Wikipedia pathwaysTGFBI
Gene fusion - rearrangments
Polymorphisms : SNP, mutations, diseases
SNP Single Nucleotide Polymorphism (NCBI)TGFBI
SNP (GeneSNP Utah)TGFBI
SNP : HGBaseTGFBI
Genetic variants : HAPMAPTGFBI
1000_GenomesTGFBI 
ICGC programENSG00000120708 
CONAN: Copy Number AnalysisTGFBI 
Somatic Mutations in Cancer : COSMICTGFBI 
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD - Leiden Open Variation Database
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
DECIPHER (Syndromes)5:135364584-135399507
Mutations and Diseases : HGMDTGFBI
OMIM121820    121900    122200    601692    602082    607541    608470    608471   
MedgenTGFBI
GENETestsTGFBI
Disease Genetic AssociationTGFBI
Huge Navigator TGFBI [HugePedia]  TGFBI [HugeCancerGEM]
Genomic VariantsTGFBI  TGFBI [DGVbeta]
Exome VariantTGFBI
dbVarTGFBI
ClinVarTGFBI
snp3D : Map Gene to Disease7045
DGIdb (Curated mutations)TGFBI
DGIdb (Drug Gene Interaction db)TGFBI
General knowledge
Homologs : HomoloGeneTGFBI
Homology/Alignments : Family Browser (UCSC)TGFBI
Phylogenetic Trees/Animal Genes : TreeFamTGFBI
Chemical/Protein Interactions : CTD7045
Chemical/Pharm GKB GenePA36484
Clinical trialTGFBI
Cancer Resource (Charite)ENSG00000120708
Other databases
Probes
Litterature
PubMed185 Pubmed reference(s) in Entrez
CoreMineTGFBI
GoPubMedTGFBI
iHOPTGFBI

Bibliography

cDNA cloning and sequence analysis of beta ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta.
Skonier J, Neubauer M, Madisen L, Bennett K, Plowman GD, Purchio AF.
DNA Cell Biol. 1992 Sep;11(7):511-22.
PMID 1388724
 
Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/beta ig-h3) that binds to collagen.
Hashimoto K, Noshiro M, Ohno S, Kawamoto T, Satakeda H, Akagawa Y, Nakashima K, Okimura A, Ishida H, Okamoto T, Pan H, Shen M, Yan W, Kato Y.
Biochim Biophys Acta. 1997 Mar 1;1355(3):303-14.
PMID 9061001
 
The transforming growth factor-beta-inducible matrix protein (beta)ig-h3 interacts with fibronectin.
Billings PC, Whitbeck JC, Adams CS, Abrams WR, Cohen AJ, Engelsberg BN, Howard PS, Rosenbloom J.
J Biol Chem. 2002 Aug 2;277(31):28003-9. Epub 2002 May 28.
PMID 12034705
 
Induction and expression of betaig-h3 in pancreatic cancer cells.
Schneider D, Kleeff J, Berberat PO, Zhu Z, Korc M, Friess H, Buchler MW.
Biochim Biophys Acta. 2002 Oct 9;1588(1):1-6.
PMID 12379307
 
Keratoepithelin suppresses the progression of experimental human neuroblastomas.
Becker J, Erdlenbruch B, Noskova I, Schramm A, Aumailley M, Schorderet DF, Schweigerer L.
Cancer Res. 2006 May 15;66(10):5314-21.
PMID 16707457
 
TGFBI gene mutations in corneal dystrophies.
Kannabiran C, Klintworth GK.
Hum Mutat. 2006 Jul;27(7):615-25. Review
PMID 16683255
 
Beta ig-h3 interacts directly with biglycan and decorin, promotes collagen VI aggregation, and participates in ternary complexing with these macromolecules.
Reinboth B, Thomas J, Hanssen E, Gibson MA.
J Biol Chem. 2006 Mar 24;281(12):7816-24. Epub 2006 Jan 24.
PMID 16434404
 
Loss of Betaig-h3 protein is frequent in primary lung carcinoma and related to tumorigenic phenotype in lung cancer cells.
Zhao Y, El-Gabry M, Hei TK.
Mol Carcinog. 2006 Feb;45(2):84-92.
PMID 16329146
 
The extracellular matrix protein TGFBI induces microtubule stabilization and sensitizes ovarian cancers to paclitaxel.
Ahmed AA, Mills AD, Ibrahim AE, Temple J, Blenkiron C, Vias M, Massie CE, Iyer NG, McGeoch A, Crawford R, Nicke B, Downward J, Swanton C, Bell SD, Earl HM, Laskey RA, Caldas C, Brenton JD.
Cancer Cell. 2007 Dec;12(6):514-27.
PMID 18068629
 
Expression patterns of betaig-h3 in chondrocyte differentiation during endochondral ossification.
Han MS, Kim JE, Shin HI, Kim IS.
Exp Mol Med. 2008 Aug 31;40(4):453-60.
PMID 18779658
 
TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions.
Irigoyen M, Anso E, Salvo E, de las Herrerias JD, Martinez-Irujo JJ, Rouzaut A.
Cell Mol Life Sci. 2008 Jul;65(14):2244-55.
PMID 18560760
 
Transforming growth factor-beta-induced gene product, as a novel ligand of integrin alphaMbeta2, promotes monocytes adhesion, migration and chemotaxis.
Kim HJ, Kim IS.
Int J Biochem Cell Biol. 2008;40(5):991-1004. Epub 2007 Nov 13.
PMID 18083624
 
Extracellular matrix protein betaig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation.
Ma C, Rong Y, Radiloff DR, Datto MB, Centeno B, Bao S, Cheng AW, Lin F, Jiang S, Yeatman TJ, Wang XF.
Genes Dev. 2008 Feb 1;22(3):308-21.
PMID 18245446
 
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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Contributor(s)

Written02-2009Chaoyu Ma, Xiao-Fan Wang
Dept of Pharmacology & Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA

Citation

This paper should be referenced as such :
Ma, C ; Wang, XF
TGFBI (transforming growth factor, beta-induced, 68kDa)
Atlas Genet Cytogenet Oncol Haematol. 2010;14(1):-.
Free online version   Free pdf version   [Bibliographic record ]
URL : http://AtlasGeneticsOncology.org/Genes/TGFBIID42539ch5q31.html

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indexed on : Thu Dec 4 15:35:03 CET 2014

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