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JAG1 jagged 1 (Alagille syndrome)

Written2005-10Michèle Meunier-Rotival, Catherine Driancourt, Julie Boyer-Di Ponio
INSERM E0020 80 rue du General Leclerc, F-94276 Le Kremlin-Bicetre Cedex, France

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Identity

Other aliasJAGGED1
HJ1
hJ1
JAGL1
LocusID (NCBI) 182
Atlas_Id 41029
Location 20p12.2  [Link to chromosome band 20p12]
Location_base_pair Starts at and ends at bp from pter
Local_order telomere PLCB1, PLCB4, PAK7, SNAP25, MKKS, JAG1 centromere
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)
JAG1 (20p12.2) / HOOK3 (8p11.21)JAG1 (20p12.2) / JAG1 (20p12.2)SRRM2 (16p13.3) / JAG1 (20p12.2)

DNA/RNA

 
  Table 1. Polymorphisms in the cDNA of JAG1. GenBank Accession no : HSU73936.
Description The gene spans 36 kb on the short arm of chromosome 20. It contains 26 exons (size from 28 bp to 2 kb) and 25 introns (size from 89 bp to nearly 9 kb) : table 1. Intron 19 contains a CA dinucleotide repeat which is a highly polymorphic marker : D20S1154 (12 alleles with heterozygosity of 85.8% and PIC of 0.844).

Size of exons and introns of the human JAG1 gene
exon 1 : 494; intron 1 : 443; exon 2 : 306; intron 2 : 8686; exon 3 : 52; intron 3 : 5240; exon 4 : 255; intron 4 : 2009; exon 5 : 61; intron 5 : 3799; exon 6 : 131; intron 6 : 217; exon 7 : 120; intron 7 : 436; exon 8 : 114; intron 8 : 1220; exon 9 : 114; intron 9 : 611; exon 10 : 114; intron 10: 414; exon 11 : 47; intron 11 : 338; exon 12 : 174; intron 12 : 438; exon 13 : 151; intron 13 : 856; exon 14 : 165; intron 14 : 854; exon 15 : 114; intron 15 : 501; exon 16 : 114; intron 16 99; exon 17 : 114; intron 17 : 163; exon 18 : 117; intron 18 : 478; exon 19 : 28; intron 19 : 493; exon 20 : 86; intron 20 : 1176; exon 21 : 114; intron 21 : 595; exon 22 : 110; intron 22 : 89; exon 23 : 234; intron 23 : 215; exon 24 : 132; intron 24 : 179; exon 25 : 151; intron 25 : 827; exon 26 : 1979

Polymorphisms were described in the cDNA sequence (table 1).

Transcription JAG1 is transcribed from centromere to telomere. The 26 exons are coding ; exon 1 is coding on the last 81 bases, and exon 26 on the first 455 bases. The transcript size is 5.5 kb.

Protein

 
  Figure 1. Schematic representation of the JAG1 protein (1218 amino acids). It contains signal peptide : SP (1-33), delta, serrate, lag-2 domain : DSL (185-229), 16 EGF-like repeats (230-856 ; cf table 3), cysteine-rich region : CR (863-1002), transmembrane domain : TM (1068-1093), intracellular (cytoplasmic) part : IC (1094-1218).
Table 2. EGF-like repeats of the human JAG1 protein. A : the 16 EGF motifs are aligned. A 24-amino acid insertion is present in EGF10 (in grey, as in human JAG2 protein). The numbers above the sequences refer to cysteine residues (C in blue). Each EGF-like repeat contains 6 cysteine residues, able to make disulfide bond bridges : 1st with 3th ; 2nd with 4th and 5th with 6th. Some of these repeats are calcium-binding EGF-like domains, which have at their amino-terminus, negatively charged or polar residues such as aspartic acid (D), glutamic acid (E), glutamine (Q), and asparagine (N). B : consensus sequence of an EGF-like repeat. x is any amino acid. Three glycine (G) residues are conserved (in green). The amino acid Z (in yellow) could be either phenylalanine (F), tryptophan (W), tyrosine (Y) or histidine (H).
Description glycosylated transmembrane protein ; 1218 amino acids,
predicted glycosylation sites : 960; 991; 1045; 1064.
Apparent size on Western blot : about 180 kDa.
Expression very wide; in heart, arteries, kidney, lung, pancreas, skeletal muscle, central nervous system, limb bud, etc. during embryonic and fetal development ; in adult tissues ; in tumors.
Localisation transmembrane plasma protein
Function ligand of the NOTCH family of receptors. The Notch signaling pathway plays a crucial role during embryonic pattern formation, controls many conserved cell determination events and defines a fundamental mechanism controlling cell fate. It is involved in lineage cell decisions in a variety of tissues. It plays a role in hematopoiesis, vascular development and angiogenesis, myogenesis, neurogenesis, somitogenesis ; kidney, eye, ear, and tooth development etc.
Homology
  • serrate in D. melanogaster
  • lag-2 in C. elegans
  • jagged 1a and jagged 1b in zebrafish (D. rerio)
  • jagged2 ou serrateB in zebrafish (D. rerio)
  • X-serrate-1 in tadpole (Xenopus laevis)
  • C-serrate-1 and C-serrate-2 in chicken (Gallus gallus)
  • jagged1 and jagged2 in mouse (Mus musculus)
  • jagged1 and jagged2 in rat (Rattus norvegicus)
  • jagged1 and jagged2 in dog (Canis familiaris)
  • partial jagged1 in Bos taurus JAGGED2 in Homo sapiens
  • Mutations

    Note Heterozygous mutations in JAG1 gene cause Alagille syndrome.
    Five per cent are deletions on the short arm of chromosome 20 that could be visible in cytogenetics : the whole gene or part of the gene, or a region larger than the gene can be deleted. : del(20p), del(20)(p11.2), del(20)(p12.3-p11.23), del(20)(p13-p12.2), ins(7;20), t(2;20).
    Ninety five per cent are point intragenic mutations that are spread over the entire gene, with the exception of the part of the gene encoding the intracellular part of the protein (see the structure of the protein in Figure 2). Seventy per cent of mutations are nonsense or frameshift mutations leading to premature stop codons ; 15% are missense mutations and 14% are splice site mutations (Figure 3). The most frequent mutation ( "delCAGT" in exon 17) accounts for 5% of all mutations.
    Some AGS probands present with no mutation in the DNA of the 26 exons and exon boundaries of JAG1. In those instances, no prenatal diagnosis can be performed.
     
      Figure 3. Distribution of 344 intragenic JAG1 mutations in Alagille patients. We summarized all the mutations published so far and unpublished results from our laboratory and from A. Mantel (Hospital of Kremlin-Bicêtre). Seventy five per cent mutations (257/344) are different at the DNA level. Sequencing exons 2, 4, 6, 9, 17, 23, and 24 which correspond to 35% of cDNA, detect 53% of all mutations.
    The signal peptide is encoded by exon 1, and the DSL domain by part of exon 4, the 16 EGF-like repeats correspond to exons 5-21, the cysteine-rich region partially overlaps exons 22-24, and the transmembrane domain and the intracellular region are encoded by exon 26.
    Germinal Most mutations (70%) are de novo
    Somatic Cases of mosaicisms are described

    Implicated in

    Note
      
    Entity Alagille syndrome (AGS)
    Disease syndrome associating 5 major features (complete syndrome) : paucity of interlobular bile ducts, pulmonary artery stenosis, butterfly-like vertebrae, posterior embryotoxon and a peculiar face. Only the 2 first ones are symptomatic. Incomplete syndrome is very frequent. AGS presents with a highly variable expressivity and nearly complete penetrance.
      
      
    Entity Tetralogy of Fallot
    Disease The heterozygous mutation (G274D) in EGF2 of JAG1 has been reported in one family : affected family members also had characteristic facies.
      
      
    Entity Familial deafness, congenital heart defects, and posterior embryotoxon
    Disease The heterozygous mutation (C234Y) in EGF1 of JAG1 has been reported in one family.
      

    Bibliography

    Syndromic paucity of interlobular bile ducts (Alagille syndrome or arteriohepatic dysplasia): review of 80 cases.
    Alagille D, Estrada A, Hadchouel M, Gautier M, Odièvre M, Dommergues JP
    The Journal of pediatrics. 1987 ; 110 (2) : 195-200.
    PMID 3806290
     
    Alagille syndrome and deletion of 20p.
    Anad F, Burn J, Matthews D, Cross I, Davison BC, Mueller R, Sands M, Lillington DM, Eastham E
    Journal of medical genetics. 1990 ; 27 (12) : 729-737.
    PMID 2074558
     
    Expression of mutant JAGGED1 alleles in patients with Alagille syndrome.
    Boyer J, Crosnier C, Driancourt C, Raynaud N, Gonzales M, Hadchouel M, Meunier-Rotival M
    Human genetics. 2005 ; 116 (6) : 445-453.
    PMID 15772854
     
    Fifteen novel mutations in the JAGGED1 gene of patients with Alagille syndrome.
    Crosnier C, Driancourt C, Raynaud N, Hadchouel M, Meunier-Rotival M
    Human mutation. 2001 ; 17 (1) : 72-73.
    PMID 11139247
     
    The DNA sequence and comparative analysis of human chromosome 20.
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    Nature. 2001 ; 414 (6866) : 865-871.
    PMID 11780052
     
    Familial Tetralogy of Fallot caused by mutation in the jagged1 gene.
    Eldadah ZA, Hamosh A, Biery NJ, Montgomery RA, Duke M, Elkins R, Dietz HC
    Human molecular genetics. 2001 ; 10 (2) : 163-169.
    PMID 11152664
     
    Parental mosaicism of JAG1 mutations in families with Alagille syndrome.
    Giannakudis J, Röpke A, Kujat A, Krajewska-Walasek M, Hughes H, Fryns JP, Bankier A, Amor D, Schlicker M, Hansmann I
    European journal of human genetics : EJHG. 2001 ; 9 (3) : 209-216.
    PMID 11313761
     
    DHPLC mutation analysis of Jagged1 (JAG1) reveals six novel mutations in Australian alagille syndrome patients.
    Heritage ML, MacMillan JC, Anderson GJ
    Human mutation. 2002 ; 20 (6) : page 481.
    PMID 12442286
     
    JAGGED1 expression in human embryos: correlation with the Alagille syndrome phenotype.
    Jones EA, Clement-Jones M, Wilson DI
    Journal of medical genetics. 2000 ; 37 (9) : 658-662.
    PMID 10978356
     
    Twelve novel JAG1 gene mutations in Polish Alagille syndrome patients.
    Jurkiewicz D, Popowska E, Gläser C, Hansmann I, Krajewska-Walasek M
    Human mutation. 2005 ; 25 (3) : page 321.
    PMID 15712272
     
    Spectrum and frequency of jagged1 (JAG1) mutations in Alagille syndrome patients and their families.
    Krantz ID, Colliton RP, Genin A, Rand EB, Li L, Piccoli DA, Spinner NB
    American journal of human genetics. 1998 ; 62 (6) : 1361-1369.
    PMID 9585603
     
    Familial deafness, congenital heart defects, and posterior embryotoxon caused by cysteine substitution in the first epidermal-growth-factor-like domain of jagged 1.
    Le Caignec C, Lefevre M, Schott JJ, Chaventre A, Gayet M, Calais C, Moisan JP
    American journal of human genetics. 2002 ; 71 (1) : 180-186.
    PMID 12022040
     
    Alagille syndrome is caused by mutations in human Jagged1, which encodes a ligand for Notch1.
    Li L, Krantz ID, Deng Y, Genin A, Banta AB, Collins CC, Qi M, Trask BJ, Kuo WL, Cochran J, Costa T, Pierpont ME, Rand EB, Piccoli DA, Hood L, Spinner NB
    Nature genetics. 1997 ; 16 (3) : 243-251.
    PMID 9207788
     
    Conditional JAG1 mutation shows the developing heart is more sensitive than developing liver to JAG1 dosage.
    Lu F, Morrissette JJ, Spinner NB
    American journal of human genetics. 2003 ; 72 (4) : 1065-1070.
    PMID 12649809
     
    Defective intracellular transport and processing of JAG1 missense mutations in Alagille syndrome.
    Morrissette JD, Colliton RP, Spinner NB
    Human molecular genetics. 2001 ; 10 (4) : 405-413.
    PMID 11157803
     
    Identification and cloning of the human homolog (JAG1) of the rat Jagged1 gene from the Alagille syndrome critical region at 20p12.
    Oda T, Elkahloun AG, Meltzer PS, Chandrasekharappa SC
    Genomics. 1997 ; 43 (3) : 376-379.
    PMID 9268641
     
    Identification of 36 novel Jagged1 (JAG1) mutations in patients with Alagille syndrome.
    Röpke A, Kujat A, Gräber M, Giannakudis J, Hansmann I
    Human mutation. 2003 ; 21 (1) : page 100.
    PMID 12497640
     
    Jagged1 mutations in alagille syndrome.
    Spinner NB, Colliton RP, Crosnier C, Krantz ID, Hadchouel M, Meunier-Rotival M
    Human mutation. 2001 ; 17 (1) : 18-33.
    PMID 11139239
     
    The DSL domain in mutant JAG1 ligand is essential for the severity of the liver defect in Alagille syndrome.
    Yuan ZR, Okaniwa M, Nagata I, Tazawa Y, Ito M, Kawarazaki H, Inomata Y, Okano S, Yoshida T, Kobayashi N, Kohsaka T
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    Citation

    This paper should be referenced as such :
    Meunier-Rotival, M ; Driancourt, C ; Boyer-Di, Ponio J
    JAG1 (jagged 1 (Alagille syndrome))
    Atlas Genet Cytogenet Oncol Haematol. 2006;10(2):79-82.
    Free journal version : [ pdf ]   [ DOI ]
    On line version : http://AtlasGeneticsOncology.org/Genes/JAG1ID41029ch20p12.html


    Other Solid tumors implicated (Data extracted from papers in the Atlas) [ 1 ]
      Breast: Ductal carcinoma


    Other Cancer prone implicated (Data extracted from papers in the Atlas) [ 1 ]
      Alagille syndrome (AGS)


    External links

    Nomenclature
    Cards
    AtlasJAG1ID41029ch20p12.txt
    Aliases
    Genomic and cartography
    Gene and transcription
    RefSeq transcript (Entrez)
    RefSeq genomic (Entrez)
    SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
    BioGPS (Tissue expression)182
    Protein : pattern, domain, 3D structure
    Domain families : Pfam (Sanger)
    Domain families : Pfam (NCBI)
    Protein Interaction databases
    Ontologies - Pathways
    Clinical trials, drugs, therapy
    Miscellaneous
    canSAR (ICR) (select the gene name)
    Probes
    Litterature
    REVIEW articlesautomatic search in PubMed
    Last year publicationsautomatic search in PubMed


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    indexed on : Thu Oct 18 17:40:19 CEST 2018

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