| Written | 2008-08 | Serkan Tuna, Ayse Elif Erson |
| Department of Biology, Middle East Technical University, Ankara, Turkey |
| Identity |
| Alias (NCBI) | MIRN125A (microRNA 125a) | hsa-mir-125a | miR-125a |
| HGNC (Hugo) | MIR125A |
| HGNC Alias symb | hsa-mir-125a |
| HGNC Previous name | MIRN125A |
| LocusID (NCBI) | 406910 |
| Atlas_Id | 44325 |
| Location | 19q13.41 [Link to chromosome band 19q13] |
| Location_base_pair | Starts at 51693254 and ends at 51693339 bp from pter ( according to hg19-Feb_2009) [Mapping MIR125A.png] |
| Local_order | Based on Mapviewer (Master Map: Genes on sequence), genes flanking miR-125a oriented from centromere to telomere on 19q13.33 are: SIGLEC5 (19q13.3): Sialic acid binding Ig-like lectin 5 SIGLEC14 (19q13.4): Sialic acid binding Ig-like lectin 14 miR-99B (19q13.33): microRNA 99b miR-LET7E (19q13.33): microRNA let-7e miR-125a (19q13.33): microRNA 125a LOC147650 (19q13.33): Hypothetical protein LOC147650 HAS1 (19q13.4): Hyaluronan Synthase 1 FPR1 (19q13.4): Formyl Peptide Receptor 1 FPRL1 (19q13.3-q13.4): Formyl Peptide Receptor-like 1 FPRL2 (19q13.3-q13.4): Formyl peptide receptor-like 2 |
| Fusion genes (updated 2017) | Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands) |
| DNA/RNA |
 | |
| Stem-loop structure of miR-125a | |
| Description | miR-125a gene is located in an intergenic region and it is close to let-7e and miR-99B. The gene cluster coordinates are: miR-99B 19: 56887677-56887746 [+] let-7e 19: 56887851-56887929 [+] miR-125a 19: 56888319-56888404 [+] |
| Transcription | MicroRNA genes are generally transcribed by RNA Pol II but can also be transcribed by RNA Pol III, if located downstream of repetitive Alu elements, 5S rRNA, tRNA and U6 snRNA genes. Transcription start site is not known for this microRNA. miR-125a is transcribed as a cluster with let-7e and miR-99b. Pre-miRNA Pre-miR Length: 86 bases Mature miR-125a |
| Pseudogene | No reported pseudogenes. |
| Protein |
| Note | miRNAs are not translated into amino acids. |
| Implicated in |
| Note | |
| Entity | Breast Cancer |
| Note | miR-125a and its homolog miR-125b were identified to be significantly downregulated in ERBB2-amplified and overexpressing breast cancers. Ectopic expression of miR-125a and miR-125b in the ERBB2 dependent human breast cancer line, SKBR3, caused suppression of its anchorage-dependent growth and inhibition of its mobility and invasive capabilities. Ectopic expression miR-125a and miR-125b in non-transformed and ERBB2-independent MCF10a cells produced inhibitory effects on its anchorage-dependent growth and no significant impact on the mobility of these non-invasive human breast epithelial cells. Furthermore, miR-125a and miR-125b targets, ERBB2 and ERBB3, were downregulated when these two microRNAs were expressed in SKBR3 cells. Downregulation of ERBB2 and ERBB3 decreased the motility and invasiveness features of SKBR3 cells. |
| Entity | Prostate Cancer |
| Note | MicroRNA levels were examined by microarrays in 10 benign peripheral zone tissues and 16 prostate cancer tissues. Widespread downregulation of miR-125b was shown in prostate cancer tissues. These results were also verified by qRT-PCR. Among 328 known and 152 novel human microRNAs, miR-125b was one of the most downregulated microRNAs in prostate cancer. Some bioinformatically predicted targets of miR-125b were found to be upregulated in prostate cancer, shown by microarray analysis ( EIF4EBP1, RPL29, MGC16063 and PAPB) and immunohistochemistry ( RAS, E2F3, BCL-2 and MCL-1). Increased expression EIF4EBP1 was also confirmed through qRT-PCR, in 61 human prostate tumors and 19 normal tissues. Several microRNA paralogous groups, having high levels of sequence similarity, were also found to be downregulated in prostate cancer. Along with miR-125a, and miR-125b, other members of let-7 family microRNAs were also downregulated. This finding indicated that these microRNAs with similar sequences might potentially target similar mRNAs. |
| Entity | Neuroblastoma |
| Note | miR-125a and miR-125b transcription was elevated in response to retinoic acid (RA) treatment in human neuroblastoma cell line (SK-N-BE), confirmed by Northern blot and qRT-PCR. Neurotrophin Receptor Tropomyosin-Related Kinase C ( NTRK3 ) is a key regulator protein of the neuroblastoma cell proliferation. Only the truncated form of NTRK3 was found to be a target of both miR-125a and miR-125b. Downregulation of tNTRK3 is critical for growth of neuroblastoma cells. Ectopic expression of miR-125a and miR-125b in primary neuroblastoma cells, (SK-N-BE), resulted in the downregulation of tNTRK3. Downregulation of these microRNAs in neuroblastoma cells resulted in tumor formation whereas upregulation of them resulted in in-vitro neuronal differentiation. |
| Bibliography |
| RNA polymerase III transcribes human microRNAs. |
| Borchert GM, Lanier W, Davidson BL. |
| Nat Struct Mol Biol. 2006 Dec;13(12):1097-101. Epub 2006 Nov 12. |
| PMID 17099701 |
| The interplay between microRNAs and the neurotrophin receptor tropomyosin-related kinase C controls proliferation of human neuroblastoma cells. |
| Laneve P, Di Marcotullio L, Gioia U, Fiori ME, Ferretti E, Gulino A, Bozzoni I, Caffarelli E. |
| Proc Natl Acad Sci U S A. 2007 May 8;104(19):7957-62. Epub 2007 May 1. |
| PMID 17483472 |
| Widespread deregulation of microRNA expression in human prostate cancer. |
| Ozen M, Creighton CJ, Ozdemir M, Ittmann M. |
| Oncogene. 2008 Mar 13;27(12):1788-93. Epub 2007 Sep 24. |
| PMID 17891175 |
| Coordinate suppression of ERBB2 and ERBB3 by enforced expression of micro-RNA miR-125a or miR-125b. |
| Scott GK, Goga A, Bhaumik D, Berger CE, Sullivan CS, Benz CC. |
| J Biol Chem. 2007 Jan 12;282(2):1479-86. Epub 2006 Nov 16. |
| PMID 17110380 |
| Citation |
| This paper should be referenced as such : |
| Tuna, S ; Erson, AE |
| MIR125A (microRNA 125a) |
| Atlas Genet Cytogenet Oncol Haematol. 2009;13(7):485-486. |
| Free journal version : [ pdf ] [ DOI ] |
| External links |
| REVIEW articles | automatic search in PubMed |
| Last year publications | automatic search in PubMed |
| © Atlas of Genetics and Cytogenetics in Oncology and Haematology | indexed on : Mon Sep 14 14:50:05 CEST 2020 |
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