| Note | Ninein (GSK3B interacting protein) |
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| | Schematic representation of NIN. LZ: leuzine-zipper domain. Coiled-coil domains are indicated with asterisks. |
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| Description | Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain, ait also interacts with C14ORF166 preventing its phosphorylation by GSK3-beta. NIN is a component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. In interphase cells, it is localized in the centrosome. Decreases in metaphase and anaphase and reappears in telophase. |
| Expression | Ubiquitous. Highly expressed in heart and skeletal muscle. |
| Localisation | Centrosome. |
| Function | The centrosome is the major microtubulin-organizing center (MTOC) in most vertebrate cells. In a typical somatic cell, the centrosome is composed of a pair of centrioles surrounded by a mass of amorphous pericentriolar material (PCM) that may be involved in the complex of Y-tubulin, centrin, pericentrin and ninein. This protein play an important role as a microtubule minus-end capping, centriole position, anchoring factor and as a centrosome maturation factor. Localization of this protein to the centrosome requires three of its four leucine zippers in the central coiled-coil domain. In addition it has been suggested that ninein could play a role to ensure equal chromosome segregation prior to telophase/cytokinesis in the division cycle. |
| Homology | It presents homology in various species. It also belongs to ENSF00000001557 protein family. |
| Pericentrin, a highly conserved centrosome protein involved in microtubule organization. |
| Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M |
| Cell. 1994 ; 76 (4) : 639-650. |
| PMID 8124707 |
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| Molecular characterisation of ninein, a new coiled-coil protein of the centrosome. |
| Bouckson-Castaing V, Moudjou M, Ferguson DJ, Mucklow S, Belkaid Y, Milon G, Crocker PR |
| Journal of cell science. 1996 ; 109 ( Pt 1) : 179-190. |
| PMID 8834802 |
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| Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta. |
| Hong YR, Chen CH, Chang JH, Wang S, Sy WD, Chou CK, Howng SL |
| Biochimica et biophysica acta. 2000 ; 1492 (2-3) : 513-516. |
| PMID 11004522 |
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| Genomic organization and molecular characterization of the human ninein gene. |
| Hong YR, Chen CH, Chuo MH, Liou SY, Howng SL |
| Biochemical and biophysical research communications. 2000 ; 279 (3) : 989-995. |
| PMID 11162463 |
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| Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein. |
| Mogensen MM, Malik A, Piel M, Bouckson-Castaing V, Bornens M |
| Journal of cell science. 2000 ; 113 ( Pt 17) : 3013-3023. |
| PMID 10934040 |
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| A subset of centrosomal proteins are arranged in a tubular conformation that is reproduced during centrosome duplication. |
| Ou Y, Rattner JB |
| Cell motility and the cytoskeleton. 2000 ; 47 (1) : 13-24. |
| PMID 11002307 |
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| Re-evaluating centrosome function. |
| Doxsey S |
| Nature reviews. Molecular cell biology. 2001 ; 2 (9) : 688-698. |
| PMID 11533726 |
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| Assembly of centrosomal proteins and microtubule organization depends on PCM-1. |
| Dammermann A, Merdes A |
| The Journal of cell biology. 2002 ; 159 (2) : 255-266. |
| PMID 12403812 |
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| CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation. |
| Ou YY, Mack GJ, Zhang M, Rattner JB |
| Journal of cell science. 2002 ; 115 (Pt 9) : 1825-1835. |
| PMID 11956314 |
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| Molecular characterization of human ninein protein: two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle. |
| Chen CH, Howng SL, Cheng TS, Chou MH, Huang CY, Hong YR |
| Biochemical and biophysical research communications. 2003 ; 308 (4) : 975-983. |
| PMID 12927815 |
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| A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3beta and is highly expressed in brain tumors. |
| Howng SL, Hsu HC, Cheng TS, Lee YL, Chang LK, Lu PJ, Hong YR |
| FEBS letters. 2004 ; 566 (1-3) : 162-168. |
| PMID 15147888 |
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| Human ninein is a centrosomal autoantigen recognized by CREST patient sera and plays a regulatory role in microtubule nucleation. |
| Stillwell EE, Zhou J, Joshi HC |
| Cell cycle (Georgetown, Tex.). 2004 ; 3 (7) : 923-930. |
| PMID 15190203 |
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| NIN, a gene encoding a CEP110-like centrosomal protein, is fused to PDGFRB in a patient with a t(5;14)(q33;q24) and an imatinib-responsive myeloproliferative disorder. |
| Vizmanos JL, Novo FJ, Romˆ°n JP, Baxter EJ, Lahortiga I, Larrˆ°yoz MJ, Odero MD, Giraldo P, Calasanz MJ, Cross NC |
| Cancer research. 2004 ; 64 (8) : 2673-2676. |
| PMID 15087377 |
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