Atlas of Genetics and Cytogenetics in Oncology and Haematology
POU2AF1 (POU domain, class 2, associating factor 1)
| Identity |
| Other names | OBF1 (Oct binding factor 1) |
| BOB1 | |
| OCA-B | |
| Hugo | POU2AF1 |
| Location | 11q23.1 |
| Local_order | telomeric to ATM |
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| POU2AF1 (11q23) - Courtesy Mariano Rocchi, Resources for Molecular Cytogenetics. Laboratories willing to validate the probes are welcome : contact | |
| DNA/RNA |
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| DNA Diagram | |
| Description | spans on a 30 kb genomic fragment; five exons; large fifth exon, with many 3' -UTR repetitive elements, two pyrimidine rich regions (a duplicated CT- rich region and a [CCTT]n tetranucleotide tandem repeat) and a 282 nucleotides long Alu element |
| Transcription | 3.4 kb mRNA; coding sequence: 770 bp, spanning from the end of exon 1 to the beginning of exon 5 |
| Protein |
| Description | 256 amino acids; 27.4 kDa; proline rich protein with no recognizable domain or motifs |
| Expression | constitutively expressed in B-cells and inducible in T-cells |
| Localisation | nuclear |
| Function | B-cell specific transcriptional coactivator: involved in the transcription of immunoglobulin genes through recruitment to the highly conserved octamer site of immunoglobulin promoters, mediated by either Oct-1 or Oct-2 transcription factor; forms a ternary complex on DNA together with either Oct-1 or Oct-2 transcription factor; is essential for the response of B-cells to antigens and is required for the formation of germinal centres no homology to known proteins. |
| Implicated in |
| Entity | t(3;11)(q27;q23.1)/ NHL --> BCL6 - OBF1 |
| Disease | NHL |
| Cytogenetics | found in complex caryotypes |
| Hybrid/Mutated Gene | 5' BOB1- 3' BCL6 and 5' BCL6 - 3'BOB1, leading to two fusion transcripts |
| Abnormal Protein | no fusion protein, but promoter exchange between both partner genes |
| External links |
| Bibliography |
| OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins. |
| Strubin M, Newell JW, Matthias P |
| Cell. 1995 ; 80 (3) : 497-506. |
| PMID 7859290 |
| A B-cell coactivator of octamer-binding transcription factors. |
| Gstaiger M, Knoepfel L, Georgiev O, Schaffner W, Hovens CM |
| Nature. 1995 ; 373 (6512) : 360-362. |
| PMID 7779176 |
| Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B. |
| Luo Y, Roeder RG |
| Molecular and cellular biology. 1995 ; 15 (8) : 4115-4124. |
| PMID 7623806 |
| Heterogeneity of breakpoints at the transcriptional co-activator gene, BOB-1, in lymphoproliferative disease. |
| Yuille MA, Galiegue-Zouitina S, Hiorns LR, Jadayel D, De Schouwer PJ, Catovsky D, Dyer MJ, Kerckaert JP |
| Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K. 1996 ; 10 (9) : 1492-1496. |
| PMID 8751468 |
| B-cell-specific coactivator OBF-1/OCA-B/Bob1 required for immune response and germinal centre formation. |
| Schubart DB, Rolink A, Kosco-Vilbois MH, Botteri F, Matthias P |
| Nature. 1996 ; 383 (6600) : 538-542. |
| PMID 8849727 |
| Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells. |
| Zwilling S, Dieckmann A, Pfisterer P, Angel P, Wirth T |
| Science (New York, N.Y.). 1997 ; 277 (5323) : 221-225. |
| PMID 9211847 |
| REVIEW articles | automatic search in PubMed |
| Last year publications | automatic search in PubMed |
| Contributor(s) |
| Written | 03-1998 | Sylvie Galiègue-Zouitina |
| Citation |
| This paper should be referenced as such : |
| Galiègue-Zouitina S . POU2AF1 (POU domain, class 2, associating factor 1). Atlas Genet Cytogenet Oncol Haematol. March 1998 . URL : http://AtlasGeneticsOncology.org/Genes/OBF94.html |
| © Atlas of Genetics and Cytogenetics in Oncology and Haematology | indexed on : Mon Jul 14 17:48:00 2008 |
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