PRXL2C (Peroxiredoxin like 2C)

2018-11-01   Jean Loup Huret 

jean-loup.huret@atlasgeneticsoncology.org

Identity

HGNC
AAED1
LOCATION
9q22.33
ALIAS
C9orf21,AAED1 (AhpC/TSA antioxidant enzyme domain containing 1)

Abstract

Review on PRXL2C, with data on DNA, on the protein encoded, and where the gene is implicated.

DNA/RNA

Atlas Image
Figure 1 PRXL2C gene

Description

Six exons, four splice forms, one of which codes for a protein:

Transcription

Coding transcript: 2863bp

Proteins

Description

A selenoprotein is a protein containing selenocysteines (Sec), with an atom of selenium SHE taking the place of the sulfur SH of the cysteine. Decoding of UGA into a selenocysteine (Sec) is alternative to the stop signal in the canonical genetic code. The human selenoproteins (SelU family) are composed of three Cys-containing members, one of which being PRXL2C (Ensembl ENSG00000158122) (Castellano et al., 2004). PRXL2C amino acids 68-71 are: CYIC, a CXXC motif.
According to InterPro http://www.ebi.ac.uk/interpro/protein/Q7RTV5 , PRXL2C signature matches with thioredoxin-like superfamily in amino acids 35-135 (or 21-90) (with a thioredoxin CXXC motif at aa 68-71 (CYIC); the two cysteines can forms a disulfide bond), and also matches with peroxiredoxin-like 2A/B/C in aa 86-196 (or 40-150). Peroxiredoxins and thioredoxins are involved in oxidation-reduction process (antioxidants). Peroxiredoxins form a family of thiol oxidoreductases and play a role in peroxides detoxification (see Figure2). A peroxidatic cysteine reduces the peroxide to water. Thioredoxin system reduce peroxiredoxins restoring to them their catalytically active form (West et al., 2018).
Atlas Image
Figure 2 Peroxides detoxification by Peroxiredoxins motifs -C-X-X-C-

Expression

PRXL2C is widely expressed (The Human Protein Atlas).

Function

Aaed1 was found to be one of the few primitive endoderm transition markers in preimplantation mouse embryos (Gerovska and Arauzo-Bravo, 2016).

Mutations

Somatic

According to Cosmic
Glioma astrocytoma grade IV amino acid (aa) mutation: p.V91A from CDS mutation c.272T>C
Papillary thyroid carcinomas aa mutation p.I81S (c.242T>G)
Thyroid carcinomas NOS aa mutations: p.V95L (c.283G>T); p.G183V (c.548G>T)
Lung small cell carcinoma aa mutation: p.E136K (c.406G>A) (Rudin et al. 2012)
Lung adenocarcinoma aa mutations: p.H145N (c.433C>A) (Imielinski et al. 2012); p.S152L (c.455C>T)
Skin melanoma aa mutations: p.M131I (c.393G>A); p.S148L (c.443C>T); p.W159* (c.476G>A)
Colon adenocarcinoma aa mutations: p.E76D (c.228G>T); p.L110Q (c.329T>A) (Giannakis et al. 2016); p.S143I (c.428G>T) (Giannakis et al. 2016); p.Q208H (c.624G>T) (Wood et al. 2007); p.P221H (c.662C>A)
Duodenum adenoma aa mutations: p.K201N (c.603A>C) (Yachida et al. 2016); p.T218A (c.652A>G) (Yachida et al. 2016)
Prostate adenocarcinoma aa mutation: p.H214R (c.641A>G)

Implicated in

Entity name
Gastric cancer
Note
(AhpC/TSA antioxidant enzyme domain containing 1), which is upregulated in gastric cancer cells. Silencing of PRXL2C inhibited cancer cell proliferation in vitro in gastric cancer cell lines. Possibly though MAPK signaling, PRXL2C upregulates HIF1A (hypoxia inducible factor 1 subunit alpha), a transcriptional activator of many genes, including glycolytic enzymes and glucose transporters in aerobic glycolysis (Zhang et al., 2018).
Entity name
Renal cancer
Note
PRXL2C High expression is related with an unfavourable prognosis according to the Human Protein Atlas https://www.proteinatlas.org/ENSG00000158122-AAED1/pathology
Entity name

Attention-deficit/hyperactivity disorder (ADHD)
Note
A rare variant of PRXL2C (rs151326868) amino acid p.H200D (c.598C>G) was found to segregate with ADHD in one of the families with an apparent dominant inheritance that was studied (Corominas et al., 2018).
PRXL2C binds PICK1 (Protein Kinase C-Alpha-Binding Protein) and PICK1 binds SLC6A3 (solute carrier family 6 member 3, also known as DAT, the dopamine transporter 1), regulating SLC6A3 trafficking in presynaptic sites of dopaminergic neurons, and DRD3 (dopamine D3 receptor 3). PICK1 also has a role in glutamate receptor regulation (Corominas et al., 2018).
Disease
ADHD is characterized by of lack of attention, impulsivity, hyperactivity and distractibility. It is a highly heritable (80%-90%) childhood behavioral disorder with a prevalence estimated at 5-7% in children, male predominance, and half of them have persisting symptoms in adulthood. In addition to genetic factors, environmental factors (adverse circumstances in maternal or children life) have been implicated in the etiology of ADHD.

Bibliography

Pubmed IDLast YearTitleAuthors
147101902004Reconsidering the evolution of eukaryotic selenoproteins: a novel nonmammalian family with scattered phylogenetic distribution.Castellano S et al
301160282020Identification of ADHD risk genes in extended pedigrees by combining linkage analysis and whole-exome sequencing.Corominas J et al
267400662016Does mouse embryo primordial germ cell activation start before implantation as suggested by single-cell transcriptomics dynamics?Gerovska D et al
271498422016Genomic Correlates of Immune-Cell Infiltrates in Colorectal Carcinoma.Giannakis M et al
229809752012Mapping the hallmarks of lung adenocarcinoma with massively parallel sequencing.Imielinski M et al
229411892012Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer.Rudin CM et al
304864892018Piecing Together How Peroxiredoxins Maintain Genomic Stability.West JD et al
179322542007The genomic landscapes of human breast and colorectal cancers.Wood LD et al
268063382016Genomic Sequencing Identifies ELF3 as a Driver of Ampullary Carcinoma.Yachida S et al
299012082018AAED1 modulates proliferation and glycolysis in gastric cancer.Zhang B et al

Citation

Jean Loup Huret

PRXL2C (Peroxiredoxin like 2C)

Atlas Genet Cytogenet Oncol Haematol. 2018-11-01

Online version: http://atlasgeneticsoncology.org/gene/60014/prxl2c