Atlas of Genetics and Cytogenetics in Oncology and Haematology

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RPS27 (ribosomal protein S27)

Written2011-08Tiffany Pierson, Brendan C Stack Jr
Department of Otolaryngology-Head, Neck Surgery, University of Arkansas for Medical Sciences, AR 72205, USA

(Note : for Links provided by Atlas : click)


Alias_namesribosomal protein S27 (metallopanstimulin 1)
Alias_symbol (synonym)MPS-1
Other alias
HGNC (Hugo) RPS27
LocusID (NCBI) 6232
Atlas_Id 45550
Location 1q21.3  [Link to chromosome band 1q21]
Location_base_pair Starts at 153990755 and ends at 153992155 bp from pter ( according to hg19-Feb_2009)  [Mapping RPS27.png]
Local_order Human RPS27 is found on chromosome 1: 153963235 - 153964626 bp from pter. Information about the local order for RPS27 can be found at Four transcripts have been identified, but only the first will be discussed below.
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)
RPS27 (1q21.3) / RPS24 (10q22.3)


Description The RPS27 gene is comprised of 1.39 kb and consists of 4 exons. This gene is a member of the Human CCDS set: CCDS1059.
Transcription The transcript is 350 base pairs long.
Pseudogene Multiple RPS27 pseudogenes are dispersed throughout the genome. The RPS27L pseudogene, located at 15q22.2, is known to encode a protein that shares 96% of its amino acid sequence with RPS27 (Balasubramanian et al., 2009).


Description RPS27 is a 9461 Da protein composed of 84 amino acids. The protein contains a C4 zinc finger domain, similar to steroid and thyroid hormones, which enables DNA binding. RPS27 is found in both the cytoplasm and the nucleus.
Expression Ubiquitous expression. Expressed at high levels in actively dividing cells and in cancers of ectodermal origin, as well as in melanoma (Fernandez-Pol et al., 1993). When overexpressed, it is secreted into serum (Lee et al., 2004).
Function 1. Component of the 40S ribosomal subunit in the cytoplasm: ribosomes carry out translation of proteins. The eukaryotic ribosome is made up of a small 40S and a large 60S subunit. Together these subunits are comprised of 4 different rRNA species and almost 80 different RP's (ribosomal proteins). As a component of the 40S subunit, RPS27 is found near RPS18 and covalently bound to translation initiation factor eIF3.
2. A mediator of cellular proliferation and survival: expression is induced by a variety of growth factors and other signaling molecules, including TGF-beta and cAMP; RPS27 can bind to cAMP response elements of DNA (Fernandez-Pol et al., 1993).
3. Oncogenesis (see below).
Homology Member of the ribosomal protein S27e family.


Note Single nucleotide polymorphisms have been identified, but have not been linked to disease.

Implicated in

Entity Various carcinomas and melanoma
Note RPS27 overexpression has been reported in many cancers including prostate cancer (Fernandez-Pol et al., 1997), colorectal cancer (Ganger et al., 1997), liver cancer (Ganger et al., 2001), breast cancer (Atsuta et al., 2002), head and neck squamous cell cancer (HNSCC) (Stack et al., 1999; Stack et al., 2004; Lee et al., 2004), gastric cancer (Wang, et al., 2006), as well as, melanoma (Santa Cruz et al., 1997).
Since high serum levels of RPS27 have been found in cancer patients, especially in head and neck squamous cell carcinoma (HNSCC), the protein can be used as a tumor marker (Fernandez-Pol et al., 1996; Lee et al., 2004; Stack et al. 2004).
Prognosis It was reported that RPS27 levels correlate with tumor stage in patients with gastric cancer, thus high levels serve as a poor prognostic indicator (Wang et al., 2006).
Oncogenesis The mechanism behind RPS27 overexpression is currently under investigation. One explanation recently offered arises from the relationship between RPS27, MDM2 and p53: RPS27 is a p53 repressible protein (He and Sun, 2007; Li et al., 2007). A 2011 study found that it competes with p53 for a central acidic binding domain on MDM2. Once bound, MDM2 is stimulated to ubiquinate and degrade the RPS27 or p53, whichever it is bound to. When RPS27 levels are elevated, it can out-compete p53 for MDM2 binding and subsequent degradation, thus stabilizing p53 levels. This would be an appropriate cellular response to genotoxic stress. The same study also found that mutant p53 cannot suppress RPS27, only the wild-type can. Since mutated p53 is found in almost 50% of all human cancers, RPS27 overexpression logically follows. Furthermore, stabilization of mutant p53 levels associated with RPS27 abundance could provide malignant cells with a growth advantage (Xiong et al., 2011).
RPS27 knockdown was found to enhance spontaneous apoptosis of tumor cells via caspase-3 activation (Wang et al., 2006; Yang et al., 2011).
HNSCC: some have questioned if RPS27 overexpression is the cause or result of cancer. A 2010 study overexpressed RPS27 in a line of HNSCC cells to study the impact on tumor behavior. They found that RPS27 overexpression resulted in reduced cancer cell growth, proliferation rate and angiogenesis. RPS27 overexpression was also found to reduce the mRNA of Paxillin, a focal adhesion protein up regulated in HNSCC and many other cancer cells. RPS27 induced Paxillin repression offers a possible explanation for the decreased HNSCC growth (Dai et al., 2010).


Identification of metallopanstimulin-1 as a member of a tumor associated antigen in patients with breast cancer.
Atsuta Y, Aoki N, Sato K, Oikawa K, Nochi H, Miyokawa N, Hirata S, Kimura S, Sasajima T, Katagiri M.
Cancer Lett. 2002 Aug 8;182(1):101-7.
PMID 12175529
Comparative analysis of processed ribosomal protein pseudogenes in four mammalian genomes.
Balasubramanian S, Zheng D, Liu YJ, Fang G, Frankish A, Carriero N, Robilotto R, Cayting P, Gerstein M.
Genome Biol. 2009;10(1):R2. Epub 2009 Jan 5.
PMID 19123937
Extraribosomal function of metallopanstimulin-1: reducing paxillin in head and neck squamous cell carcinoma and inhibiting tumor growth.
Dai Y, Pierson SE, Dudney WC, Stack BC Jr.
Int J Cancer. 2010 Feb 1;126(3):611-9.
PMID 19642098
Expression of metallopanstimulin and oncogenesis in human prostatic carcinoma.
Fernandez-Pol JA, Fletcher JW, Hamilton PD, Klos DJ.
Anticancer Res. 1997 May-Jun;17(3A):1519-30.
PMID 9179190
Differential expression of metallopanstimulin/S27 ribosomal protein in hepatic regeneration and neoplasia.
Ganger DR, Hamilton PD, Klos DJ, Jakate S, McChesney L, Fernandez-Pol JA.
Cancer Detect Prev. 2001;25(3):231-6.
PMID 11425264
MDMX regulation of p53 response to ribosomal stress.
Gilkes DM, Chen L, Chen J.
EMBO J. 2006 Nov 29;25(23):5614-25. Epub 2006 Nov 16.
PMID 17110929
Ribosomal protein S27L is a direct p53 target that regulates apoptosis.
He H, Sun Y.
Oncogene. 2007 Apr 26;26(19):2707-16. Epub 2006 Oct 23.
PMID 17057733
A new assay to screen for head and neck squamous cell carcinoma using the tumor marker metallopanstimulin.
Lee WJ, Keefer K, Hollenbeak CS, Stack BC Jr.
Otolaryngol Head Neck Surg. 2004 Oct;131(4):466-71.
PMID 15467619
Ribosomal protein S27-like, a p53-inducible modulator of cell fate in response to genotoxic stress.
Li J, Tan J, Zhuang L, Banerjee B, Yang X, Chau JF, Lee PL, Hande MP, Li B, Yu Q.
Cancer Res. 2007 Dec 1;67(23):11317-26.
PMID 18056458
Differential expression of metallopanstimulin/S27 ribosomal protein in melanocytic lesions of the skin.
Santa Cruz DJ, Hamilton PD, Klos DJ, Fernandez-Pol JA.
J Cutan Pathol. 1997 Oct;24(9):533-42. (REVIEW)
PMID 9404850
Metallopanstimulin as a marker for head and neck cancer.
Stack BC Jr, Hollenbeak CS, Lee CM, Dunphy FR, Lowe VJ, Hamilton PD.
World J Surg Oncol. 2004 Dec 14;2:45.
PMID 15598348
In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity.
Wang YW, Qu Y, Li JF, Chen XH, Liu BY, Gu QL, Zhu ZG.
Clin Cancer Res. 2006 Aug 15;12(16):4965-73.
PMID 16914586
Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator.
Xiong X, Zhao Y, He H, Sun Y.
Oncogene. 2011 Apr 14;30(15):1798-811. Epub 2010 Dec 20.
PMID 21170087
Knockdown of metallopanstimulin-1 inhibits NF-?B signaling at different levels: The role of apoptosis induction of gastric cancer cells.
Yang ZY, Qu Y, Zhang Q, Wei M, Liu CX, Chen XH, Yan M, Zhu ZG, Liu BY, Chen GQ, Wu YL, Gu QL.
Int J Cancer. 2011 Jul 27. doi: 10.1002/ijc.26331. [Epub ahead of print]
PMID 21796632


This paper should be referenced as such :
Pierson, T ; Stack, BCJr
RPS27 (ribosomal protein S27)
Atlas Genet Cytogenet Oncol Haematol. 2012;16(2):103-105.
Free journal version : [ pdf ]   [ DOI ]
On line version :

External links

HGNC (Hugo)RPS27   10416
LRG (Locus Reference Genomic)LRG_1158
Entrez_Gene (NCBI)RPS27  6232  ribosomal protein S27
AliasesDBA17; MPS-1; MPS1; S27
GeneCards (Weizmann)RPS27
Ensembl hg19 (Hinxton)ENSG00000177954 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000177954 [Gene_View]  chr1:153990755-153992155 [Contig_View]  RPS27 [Vega]
ICGC DataPortalENSG00000177954
TCGA cBioPortalRPS27
AceView (NCBI)RPS27
Genatlas (Paris)RPS27
SOURCE (Princeton)RPS27
Genetics Home Reference (NIH)RPS27
Genomic and cartography
GoldenPath hg38 (UCSC)RPS27  -     chr1:153990755-153992155 +  1q21.3   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)RPS27  -     1q21.3   [Description]    (hg19-Feb_2009)
EnsemblRPS27 - 1q21.3 [CytoView hg19]  RPS27 - 1q21.3 [CytoView hg38]
Mapping of homologs : NCBIRPS27 [Mapview hg19]  RPS27 [Mapview hg38]
OMIM603702   617409   
Gene and transcription
Genbank (Entrez)AJ572583 AK312070 AV763942 BC002658 BC070219
RefSeq transcript (Entrez)NM_001030 NM_001349946 NM_001349947
RefSeq genomic (Entrez)
Consensus coding sequences : CCDS (NCBI)RPS27
Cluster EST : UnigeneHs.654475 [ NCBI ]
CGAP (NCI)Hs.654475
Alternative Splicing GalleryENSG00000177954
Gene ExpressionRPS27 [ NCBI-GEO ]   RPS27 [ EBI - ARRAY_EXPRESS ]   RPS27 [ SEEK ]   RPS27 [ MEM ]
Gene Expression Viewer (FireBrowse)RPS27 [ Firebrowse - Broad ]
SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
GenevestigatorExpression in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)6232
GTEX Portal (Tissue expression)RPS27
Human Protein AtlasENSG00000177954-RPS27 [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtP42677   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtP42677  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProP42677
Splice isoforms : SwissVarP42677
Domaine pattern : Prosite (Expaxy)RIBOSOMAL_S27E (PS01168)   
Domains : Interpro (EBI)Ribosomal_S27e    Ribosomal_S27e_Zn-bd_dom    Ribosomal_zn-bd   
Domain families : Pfam (Sanger)Ribosomal_S27e (PF01667)   
Domain families : Pfam (NCBI)pfam01667   
Domain structure : Prodom (Prabi Lyon)Ribosomal_S27e (PD004466)   
Conserved Domain (NCBI)RPS27
DMDM Disease mutations6232
Blocks (Seattle)RPS27
PDB (SRS)4UG0    4V6X    5A2Q    5AJ0    5FLX    5T2C   
PDB (PDBSum)4UG0    4V6X    5A2Q    5AJ0    5FLX    5T2C   
PDB (IMB)4UG0    4V6X    5A2Q    5AJ0    5FLX    5T2C   
PDB (RSDB)4UG0    4V6X    5A2Q    5AJ0    5FLX    5T2C   
Structural Biology KnowledgeBase4UG0    4V6X    5A2Q    5AJ0    5FLX    5T2C   
SCOP (Structural Classification of Proteins)4UG0    4V6X    5A2Q    5AJ0    5FLX    5T2C   
CATH (Classification of proteins structures)4UG0    4V6X    5A2Q    5AJ0    5FLX    5T2C   
Human Protein Atlas [tissue]ENSG00000177954-RPS27 [tissue]
Peptide AtlasP42677
Protein Interaction databases
IntAct (EBI)P42677
Ontologies - Pathways
Ontology : AmiGOribosomal small subunit assembly  nuclear-transcribed mRNA catabolic process, nonsense-mediated decay  DNA binding  RNA binding  structural constituent of ribosome  protein binding  nucleus  nucleoplasm  cytosol  ribosome  rRNA processing  rRNA processing  translation  translation  translation  translational initiation  SRP-dependent cotranslational protein targeting to membrane  sister chromatid cohesion  zinc ion binding  cell proliferation  viral transcription  cytosolic small ribosomal subunit  cytosolic small ribosomal subunit  
Ontology : EGO-EBIribosomal small subunit assembly  nuclear-transcribed mRNA catabolic process, nonsense-mediated decay  DNA binding  RNA binding  structural constituent of ribosome  protein binding  nucleus  nucleoplasm  cytosol  ribosome  rRNA processing  rRNA processing  translation  translation  translation  translational initiation  SRP-dependent cotranslational protein targeting to membrane  sister chromatid cohesion  zinc ion binding  cell proliferation  viral transcription  cytosolic small ribosomal subunit  cytosolic small ribosomal subunit  
Pathways : KEGGRibosome   
REACTOMEP42677 [protein]
REACTOME PathwaysR-HSA-975957 [pathway]   
NDEx NetworkRPS27
Atlas of Cancer Signalling NetworkRPS27
Wikipedia pathwaysRPS27
Orthology - Evolution
GeneTree (enSembl)ENSG00000177954
Phylogenetic Trees/Animal Genes : TreeFamRPS27
Homologs : HomoloGeneRPS27
Homology/Alignments : Family Browser (UCSC)RPS27
Gene fusions - Rearrangements
Fusion : QuiverRPS27
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerRPS27 [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)RPS27
Exome Variant ServerRPS27
ExAC (Exome Aggregation Consortium)ENSG00000177954
GNOMAD BrowserENSG00000177954
Genetic variants : HAPMAP6232
Genomic Variants (DGV)RPS27 [DGVbeta]
DECIPHERRPS27 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisRPS27 
ICGC Data PortalRPS27 
TCGA Data PortalRPS27 
Broad Tumor PortalRPS27
OASIS PortalRPS27 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICRPS27  [overview]  [genome browser]  [tissue]  [distribution]  
Mutations and Diseases : HGMDRPS27
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD - Leiden Open Variation Database
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
BioMutasearch RPS27
DgiDB (Drug Gene Interaction Database)RPS27
DoCM (Curated mutations)RPS27 (select the gene name)
CIViC (Clinical Interpretations of Variants in Cancer)RPS27 (select a term)
NCG5 (London)RPS27
Cancer3DRPS27(select the gene name)
Impact of mutations[PolyPhen2] [SIFT Human Coding SNP] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
OMIM603702    617409   
Genetic Testing Registry RPS27
NextProtP42677 [Medical]
Target ValidationRPS27
Huge Navigator RPS27 [HugePedia]
snp3D : Map Gene to Disease6232
BioCentury BCIQRPS27
Clinical trials, drugs, therapy
Chemical/Protein Interactions : CTD6232
Chemical/Pharm GKB GenePA34820
Clinical trialRPS27
canSAR (ICR)RPS27 (select the gene name)
PubMed70 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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