Atlas of Genetics and Cytogenetics in Oncology and Haematology

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SPA17 (sperm autoantigenic protein 17)

Written2007-09Leri S. Faried, Ahmad Faried
Department of Gynecology, Reproductive Medicine, Graduate School of Medicine, Gunma University, Japan (FLS); Department of Surgery I, Gunma University, Japan (FA).

(Note : for Links provided by Atlas : click)


Alias (NCBI)SP17
HGNC (Hugo) SPA17
HGNC Alias symbSP17
HGNC Alias namecancer/testis antigen 22
LocusID (NCBI) 53340
Atlas_Id 42360
Location 11q24.2  [Link to chromosome band 11q24]
Location_base_pair Starts at 124673844 and ends at 124694791 bp from pter ( according to GRCh38/hg38-Dec_2013)  [Mapping SPA17.png]
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)


Description The SPA17 gene contains 5 exons and 4 introns and was predicted to span over approximately 23 kb of the genomic DNA. The first exon is encodes solely the 5' untranslated sequence. The exon 2 encodes the first 51 amino acid residue. Exon 3 and 4 are only 71 and 87 bp length and contain coding region. Exon 5 contains stop codon and followed by 3' untranslated sequence. The size of introns 2, 3 and 4 were 5970, 10.235 and 2520 bp, respectively.
Transcription Two transcript variants encoding the same protein have been identified for SP17-1 gene. This variant which was named SP17-1A and SP17-1B, both cDNA encodes identical protein sequence and only differed in 5' UTR part. One hundred percent homology can be detected from the poly A tail up to SP17-1 position -62 for variant A and -31 for variant B.
Pseudogene There is one pseudogene, SP17-2 intronless gene has all the hallmark of retro-position giving rise to a pseudogene, located on chromosome 10. SP17-2 gene had 61 point mutations, 4 nucleotide insertions and 2 deletions leading to internal stop codons and frame shifts which do not result in ORF encoding a full-length SPA17 polypeptide.


Description The SPA17 consists of 151 amino acids corresponding to a molecular weight of 17.4 kDa. SPA17 protein indicated to have functional role in cell regulation by its calmodulin binding site located at its C-terminal end. The N-terminal end of SPA17, similar to human cyclic adenosine 3'-5'-monophosphate (cAMP)-dependent protein kinase type II regulatory subunits (RII), may facilitate dimmer formation. SPA17's central domain is necessary for heparin-binding and exhibits the greatest sequence divergence of all three domains. This region plays an important role in cell-cell and/or cell matrix recognition process and/or cell regulation, possibly via its interaction with extra cellular heparin sulfate.
Expression The SPA17 expression was originally found to be expressed exclusively in the testis. Recently, this gene also found in other somatic tissues.
Localisation SPA17 is present throughout the cytoplasm and is particularly concentrated around the nucleus. It's also present on the surface of B-lymphocytes and granulocytes; T-lymphocytes and monocytes; most malignant B and T lymphocytes cell lines.
Function SPA17 is highly antigenic, testes specific antigen and known have function to bind sperm to the zona pellucida of the oocyte. In addition, SPA17 may also have an alternative role in highly proliferating and neoplastic cells, such as a mediator of signal transduction, protein synthesis and/or unregulated growth.
Homology The homologues of SPA17 protein sequences were found from eutherian and marsupial mammals, including rat, mouse, macaque, baboon, human, marmoset, rabbit, Monodelphis and wallaby.

Implicated in

Entity Immune system
Disease SPA17 is a highly immunogenic protein. More than 90% of male spontaneously developed B-cell immunity of SPA17 following vasectomy. SPA17 can be easily generated SPA17-specific cytotoxic T lymphocytes (CTLs) from peripheral blood of healthy donors and also from peripheral blood of cancer-bearing patients. SPA17 have been tested as tumor vaccines in a phase I study. In addition, SPA17 gene was described in human rheumatoid arthritis (RA).
Entity Malignancy
Disease SPA17 reported to be over-expressed in female reproduction organ, multiple myeloma, esthesioneuroblastoma, nervous system tumors and esophageal cancer.
Prognosis SPA17 expression closely related with poor prognosis in malignancy.
Oncogenesis SPA17 was identified as a candidate gene related to chemoresistance of clear cell adenocarcinoma of the ovary, which has a poor prognosis due to its chemoresistancy and early metastasis to the lymph nodes.


Genomic organization of an intron-containing sperm protein 17 gene (Sp17-1) and an intronless pseudogene (Sp17-2) in humans: a new model.
Buchli R, De Jong A, Robbins DL
Biochimica et biophysica acta. 2002 ; 1578 (1-3) : 29-42.
PMID 12393185
Sperm protein 17 expression defines 2 subsets of primary esthesioneuroblastoma.
Bumm K, Grizzi F, Franceschini B, Koch M, Iro H, Wurm J, Ceva-Grimaldi G, Dimmler A, Cobos E, Dioguardi N, Sinha UK, Kast WM, Chiriva-Internati M
Human pathology. 2005 ; 36 (12) : 1289-1293.
PMID 16311122
Successful generation of sperm protein 17 (Sp17)-specific cytotoxic T lymphocytes from normal donors: implication for tumour-specific adoptive immunotherapy following allogeneic stem cell transplantation for Sp17-positive multiple myeloma.
Chiriva-Internati M, Wang Z, Salati E, Wroblewski D, Lim SH
Scandinavian journal of immunology. 2002 ; 56 (4) : 429-433.
PMID 12234264
Characterization of sperm protein 17 in human somatic and neoplastic tissue.
De Jong A, Buchli R, Robbins D
Cancer letters. 2002 ; 186 (2) : 201-209.
PMID 12213290
Sperm protein 17 is expressed in human nervous system tumours.
Grizzi F, Gaetani P, Franceschini B, Di Ieva A, Colombo P, Ceva-Grimaldi G, Bollati A, Frezza EE, Cobos E, Rodriguez y Baena R, Dioguardi N, Chiriva-Internati M
BMC cancer. 2006 ; 6 : page 23.
PMID 16438728
Clinical significance of sperm protein 17 expression and immunogenicity in esophageal cancer.
Gupta G, Sharma R, Chattopadhyay TK, Gupta SD, Ralhan R
International journal of cancer. Journal international du cancer. 2007 ; 120 (8) : 1739-1747.
PMID 17230514
Sperm protein 17 is expressed on normal and malignant lymphocytes and promotes heparan sulfate-mediated cell-cell adhesion.
Lacy HM, Sanderson RD
Blood. 2001 ; 98 (7) : 2160-2165.
PMID 11568003
Autoimmunogenicity of the human sperm protein Sp17 in vasectomized men and identification of linear B cell epitopes.
Lea IA, Adoyo P, O'Rand MG
Fertility and sterility. 1997 ; 67 (2) : 355-361.
PMID 9022615
Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17.
Lea IA, Richardson RT, Widgren EE, O'Rand MG
Biochimica et biophysica acta. 1996 ; 1307 (3) : 263-266.
PMID 8688458
Idiotypic protein-pulsed dendritic cell vaccination in multiple myeloma.
Lim SH, Bailey-Wood R
International journal of cancer. Journal international du cancer. 1999 ; 83 (2) : 215-222.
PMID 10471530
Sperm protein 17 is a novel cancer-testis antigen in multiple myeloma.
Lim SH, Wang Z, Chiriva-Internati M, Xue Y
Blood. 2001 ; 97 (5) : 1508-1510.
PMID 11222401
Sperm protein 17 influences the tissue-specific malignancy of clear cell adenocarcinoma in human epithelial ovarian cancer.
Nakazato T, Kanuma T, Tamura T, Faried LS, Aoki H, Minegishi T
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society. 2007 ; 17 (2) : 426-432.
PMID 17309563
Characterization of the rabbit sperm membrane autoantigen, RSA, as a lectin-like zona binding protein.
O'Rand MG, Widgren EE, Fisher SJ
Developmental biology. 1988 ; 129 (1) : 231-240.
PMID 3410159
Expression of sperm protein 17 (Sp17) in ovarian cancer.
Straughn JM Jr, Shaw DR, Guerrero A, Bhoola SM, Racelis A, Wang Z, Chiriva-Internati M, Grizzle WE, Alvarez RD, Lim SH, Strong TV
International journal of cancer. Journal international du cancer. 2004 ; 108 (6) : 805-811.
PMID 14712480
cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes.
Taylor SS, Buechler JA, Yonemoto W
Annual review of biochemistry. 1990 ; 59 : 971-1005.
PMID 2165385
Characterization of Sp17: a ubiquitous three domain protein that binds heparin.
Wen Y, Richardson RT, Widgren EE, O'Rand MG
The Biochemical journal. 2001 ; 357 (Pt 1) : 25-31.
PMID 11415432


This paper should be referenced as such :
Faried, LS ; Faried, A
SPA17 (sperm autoantigenic protein 17)
Atlas Genet Cytogenet Oncol Haematol. 2008;12(2):159-160.
Free journal version : [ pdf ]   [ DOI ]

External links

HGNC (Hugo)SPA17   11210
Entrez_Gene (NCBI)SPA17    sperm autoantigenic protein 17
AliasesCT22; SP17; SP17-1
GeneCards (Weizmann)SPA17
Ensembl hg19 (Hinxton)ENSG00000064199 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000064199 [Gene_View]  ENSG00000064199 [Sequence]  chr11:124673844-124694791 [Contig_View]  SPA17 [Vega]
ICGC DataPortalENSG00000064199
TCGA cBioPortalSPA17
AceView (NCBI)SPA17
Genatlas (Paris)SPA17
SOURCE (Princeton)SPA17
Genetics Home Reference (NIH)SPA17
Genomic and cartography
GoldenPath hg38 (UCSC)SPA17  -     chr11:124673844-124694791 +  11q24.2   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)SPA17  -     11q24.2   [Description]    (hg19-Feb_2009)
GoldenPathSPA17 - 11q24.2 [CytoView hg19]  SPA17 - 11q24.2 [CytoView hg38]
genome Data Viewer NCBISPA17 [Mapview hg19]  
Gene and transcription
Genbank (Entrez)AF334735 AK307271 AK311806 BC019224 BC032457
RefSeq transcript (Entrez)NM_017425
RefSeq genomic (Entrez)
Consensus coding sequences : CCDS (NCBI)SPA17
Alternative Splicing GalleryENSG00000064199
Gene ExpressionSPA17 [ NCBI-GEO ]   SPA17 [ EBI - ARRAY_EXPRESS ]   SPA17 [ SEEK ]   SPA17 [ MEM ]
Gene Expression Viewer (FireBrowse)SPA17 [ Firebrowse - Broad ]
GenevisibleExpression of SPA17 in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)53340
GTEX Portal (Tissue expression)SPA17
Human Protein AtlasENSG00000064199-SPA17 [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ15506   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtQ15506  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProQ15506
Splice isoforms : SwissVarQ15506
Domaine pattern : Prosite (Expaxy)IQ (PS50096)   
Domains : Interpro (EBI)cAMP_dep_PK_reg_su_I/II_a/b    IQ_motif_EF-hand-BS    Sp17   
Domain families : Pfam (Sanger)IQ (PF00612)    RIIa (PF02197)   
Domain families : Pfam (NCBI)pfam00612    pfam02197   
Domain families : Smart (EMBL)IQ (SM00015)  RIIa (SM00394)  
Conserved Domain (NCBI)SPA17
Blocks (Seattle)SPA17
Human Protein Atlas [tissue]ENSG00000064199-SPA17 [tissue]
Peptide AtlasQ15506
Protein Interaction databases
IntAct (EBI)Q15506
Ontologies - Pathways
Ontology : AmiGOepithelial cilium movement  protein binding  calmodulin binding  extracellular region  cytoplasm  cytoplasm  cilium  cilium  spermatogenesis  single fertilization  binding of sperm to zona pellucida  external side of plasma membrane  motile cilium  sperm fibrous sheath  sperm fibrous sheath  sperm principal piece  sperm principal piece  
Ontology : EGO-EBIepithelial cilium movement  protein binding  calmodulin binding  extracellular region  cytoplasm  cytoplasm  cilium  cilium  spermatogenesis  single fertilization  binding of sperm to zona pellucida  external side of plasma membrane  motile cilium  sperm fibrous sheath  sperm fibrous sheath  sperm principal piece  sperm principal piece  
NDEx NetworkSPA17
Atlas of Cancer Signalling NetworkSPA17
Wikipedia pathwaysSPA17
Orthology - Evolution
GeneTree (enSembl)ENSG00000064199
Phylogenetic Trees/Animal Genes : TreeFamSPA17
Homologs : HomoloGeneSPA17
Homology/Alignments : Family Browser (UCSC)SPA17
Gene fusions - Rearrangements
Fusion : Fusion_HubSC5D--SPA17    SC5DL--SPA17   
Fusion : QuiverSPA17
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerSPA17 [hg38]
Exome Variant ServerSPA17
GNOMAD BrowserENSG00000064199
Varsome BrowserSPA17
Genomic Variants (DGV)SPA17 [DGVbeta]
DECIPHERSPA17 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisSPA17 
ICGC Data PortalSPA17 
TCGA Data PortalSPA17 
Broad Tumor PortalSPA17
OASIS PortalSPA17 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICSPA17  [overview]  [genome browser]  [tissue]  [distribution]  
Somatic Mutations in Cancer : COSMIC3DSPA17
Mutations and Diseases : HGMDSPA17
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
BioMutasearch SPA17
DgiDB (Drug Gene Interaction Database)SPA17
DoCM (Curated mutations)SPA17 (select the gene name)
CIViC (Clinical Interpretations of Variants in Cancer)SPA17 (select a term)
NCG6 (London) select SPA17
Cancer3DSPA17(select the gene name)
Impact of mutations[PolyPhen2] [Provean] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
Genetic Testing Registry SPA17
NextProtQ15506 [Medical]
Target ValidationSPA17
Huge Navigator SPA17 [HugePedia]
Clinical trials, drugs, therapy
Protein Interactions : CTD
Pharm GKB GenePA36047
Clinical trialSPA17
canSAR (ICR)SPA17 (select the gene name)
DataMed IndexSPA17
PubMed39 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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indexed on : Fri Feb 19 18:00:46 CET 2021

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