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USP32 (ubiquitin specific peptidase 32)

Written2013-07Aysegul Sapmaz, Ayse Elif Erson-Bensan
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey (AS, AEEB)

(Note : for Links provided by Atlas : click)


Alias (NCBI)NY-REN-60
HGNC (Hugo) USP32
HGNC Alias symbNY-REN-60
HGNC Previous nameubiquitin specific protease 32
LocusID (NCBI) 84669
Atlas_Id 52046
Location 17q23.1  [Link to chromosome band 17q23]
Location_base_pair Starts at 60177328 and ends at 60392133 bp from pter ( according to GRCh38/hg38-Dec_2013)  [Mapping USP32.png]
Local_order Based on Mapviewer (Master Map: Gene on sequence, gene flanking USP32 oriented from centromere on 17q23.3 are:
- TBC1D3P1-DHX40P1 (17q23): TBC1D3P1-DHX40P1 read through transcribed pseudogene
- MIR4737: MicroRNA 4737
- HEATR6 (17q23.1): Heat repeat containing 6
- LOC100422693 (Chr.17): UDP-N-acetyl-alpha-D-galactosamine :polypeptide N-acetylgalactosamyltransferase 1 (GalNAc-T1) pseudogene
- LOC6456338 (17q23.1): WDNM1-like pseudogene
- LOC653653(17q23.1): adaptor-related protein complex 1 sigma 2 subunit pseudogene
- CA4 (17q23): carbonic anhydrase IV
- FAM106DP (Chr. 17): family with sequence similarity 106 memberD pseudogene
- SCARNA20 (17q23.2): small cajal body specific RNA 20
- RPL32P32 (17q23.2): ribosomal protein L32 pseudogene 32
- LOC100418753 (Chr.17): septin 7 pseudogene
- USP32 (17q23.3): ubiquitin specific protease 32
- LOC100506882 (Chr.17): uncharacterized LOC100506882
- C17orf64 (17q23.2): chromosome 17 open reading frame 64
- RPL12P38 (17q23.2): ribosomal protein L12 pseudogene 38
- HMGN2P42 (Chr.17) high mobility group nucleosomal binding domain 2 pseudogen 42
- APPBP2 (17q23.2): amyloid beta precursor protein (cytoplasmic tail binding protein 2.
  Figure 1. Genes flanking USP32 gene on 17q23.3. → stands for positive strand , ← stands for negative strand.
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)
AATK (17q25.3)::USP32 (17q23.1)CDC27 (17q21.32)::USP32 (17q23.1)CYB561 (17q23.3)::USP32 (17q23.1)
SLC25A10 (17q25.3)::USP32 (17q23.1)TUBD1 (17q23.1)::USP32 (17q23.1)USP32 (17q23.1)::APPBP2 (17q23.2)
USP32 (17q23.1)::ARHGAP39 (8q24.3)USP32 (17q23.1)::BCAS3 (17q23.2)USP32 (17q23.1)::CLTC (17q23.1)
USP32 (17q23.1)::CRYBA4 (22q12.1)USP32 (17q23.1)::CWC25 (17q12)USP32 (17q23.1)::DHX40 (17q23.1)
USP32 (17q23.1)::ELOF1 (19p13.2)USP32 (17q23.1)::EPHA1 (7q34)USP32 (17q23.1)::GPR137 (11q13.1)
USP32 (17q23.1)::MAP3K3 (17q23.3)USP32 (17q23.1)::MED1 (17q12)USP32 (17q23.1)::NLK (17q11.2)
USP32 (17q23.1)::PACSIN2 (22q13.2)USP32 (17q23.1)::PCTP (17q22)USP32 (17q23.1)::RAD51C (17q22)
USP32 (17q23.1)::UBE2O (17q25.1)USP32 (17q23.1)::UBN2 (7q34)USP32 (17q23.1)::UPF2 (10p14)
USP32 (17q23.1)::USP32 (17q23.1)USP32 (17q23.1)::ZDHHC7 (16q24.1)WIPF2 (17q21.1)::USP32 (17q23.1)
WIPF2 (17q21.2)::USP32 (17q23.1)


  Figure 2. 34 exons of USP32.
Description USP32 gene is located on a prominent gene amplification region, 17q23, in breast cancers (Bärlund et al., 1997; Erson et al., 2001) and has 34 exons (figure 2).
Transcription USP32 mRNA is 7026 bp long. Coding sequence of USP32 starts at the 287th bp and ends at the 5101st bp of the mRNA. Total length of USP32 coding sequence is 4815 bp.
Pseudogene No pseudogene has been reported for USP32.


Note Three independent studies reported USP32 to be phosphorylated at the 1173rd tyrosine and 1372nd serine residues (Dephoure et al., 2008; Rigbolt et al., 2011; Mayya et al., 2009).
Description USP32 (ubiquitin specific protease 32 - accession number: NT_010783 and mRNA accession number: NM_032582) encodes for a protein consisting of 1604 amino acids and the resulting protein's predicted molecular weight is approximately 182 kDa. The N-terminal region contains calcium binding domain with EF-hand and DUSP domains.
The EF-hand calcium binding domains, consisting of a helix (E), a loop and a second helix (F) motif, are generally found in calcium binding proteins.
DUSP domain is common among ubiquitin specific proteases. The function of this domain in USP32 remains unclear but is predicted be functional in protein-protein interactions. In addition, USP32 harbors Cys, His and Asp triad which is common in USP subfamily of DUBs (figure 3). Recently, active deubiquitination function of USP32 has been established (Akhavantabasi et al., 2010).
  Figure 3. Domains of USP32.
Expression Overexpressed transcript was detected in malignant breast ephitelium (Grigoriadis et al., 2006). Another study showed overexpression of USP32 in 50% (9 of 18) of breast cancer cell lines and 22% (9 of 41) of primary breast tumors compared to mammary epithelial cells (Akhavantabasi et al., 2010).
Localisation Golgi (Akhavantabasi et al., 2010).
Function USP32 is an active deubiquitinating enzyme (Akhavantabasi et al., 2010).
Homology C-terminal of USP32 shows 97% nucleotide homology with USP6 (ubiquitin specific protease 6 (Tre-2 oncogene)) (Paulding et al., 2003) (figure 4).


Note In the Parkinson disease, CNVs in USP32 gene were suggested. However, these variations in USP32 were not confirmed with Multiplex ligation-dependent probe amplification (MLPA) and real time PCR (Pankratz et al., 2011).

Implicated in

Entity Breast cancer
Note USP32 is located on 17q23 chromosomal region which is amplified in breast cancer (Sinclair et al., 2003; Haverty et al., 2008). Real-time PCR analysis in breast cancer cell lines determined that USP32 transcript is amplified more than two-fold in 50% of breast cancer cell lines and in 22% of (9 of 41) primary breast tumors compared to normal breast tissue samples. Moreover, silencing of USP32 leads to a decrease in the proliferation and migration properties of HeLa and MCF7 cells (Akhavantabasi et al., 2010).
In estrogen receptor (ER) positive tumors, USP32 may have a higher copy number than ER negative tumors (Zhang et al., 2009). Scafoglio et al. (2006) suggest USP32 to be an estrogen responsive gene.
Hybrid/Mutated Gene RT-PCR and transcriptome sequencing analysis determined USP32 to be one of the twelve expressed fusion genes in breast cancer cell line ZR-75-30. USP32 is found to be expressed with CCDC49 as a fusion transcript (Schulte et al., 2012).


USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers.
Akhavantabasi S, Akman HB, Sapmaz A, Keller J, Petty EM, Erson AE.
Mamm Genome. 2010 Aug;21(7-8):388-97. doi: 10.1007/s00335-010-9268-4. Epub 2010 Jun 13.
PMID 20549504
Increased copy number at 17q22-q24 by CGH in breast cancer is due to high-level amplification of two separate regions.
Barlund M, Tirkkonen M, Forozan F, Tanner MM, Kallioniemi O, Kallioniemi A.
Genes Chromosomes Cancer. 1997 Dec;20(4):372-6.
PMID 9408753
A quantitative atlas of mitotic phosphorylation.
Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP.
Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31.
PMID 18669648
Overexpressed genes/ESTs and characterization of distinct amplicons on 17q23 in breast cancer cells.
Erson AE, Niell BL, DeMers SK, Rouillard JM, Hanash SM, Petty EM.
Neoplasia. 2001 Nov-Dec;3(6):521-6.
PMID 11774034
Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data.
Grigoriadis A, Mackay A, Reis-Filho JS, Steele D, Iseli C, Stevenson BJ, Jongeneel CV, Valgeirsson H, Fenwick K, Iravani M, Leao M, Simpson AJ, Strausberg RL, Jat PS, Ashworth A, Neville AM, O'Hare MJ.
Breast Cancer Res. 2006;8(5):R56.
PMID 17014703
High-resolution genomic and expression analyses of copy number alterations in breast tumors.
Haverty PM, Fridlyand J, Li L, Getz G, Beroukhim R, Lohr S, Wu TD, Cavet G, Zhang Z, Chant J.
Genes Chromosomes Cancer. 2008 Jun;47(6):530-42. doi: 10.1002/gcc.20558.
PMID 18335499
Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK.
Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007.
PMID 19690332
Copy number variation in familial Parkinson disease.
Pankratz N, Dumitriu A, Hetrick KN, Sun M, Latourelle JC, Wilk JB, Halter C, Doheny KF, Gusella JF, Nichols WC, Myers RH, Foroud T, DeStefano AL; PSG-PROGENI and GenePD Investigators, Coordinators and Molecular Genetic Laboratories.
PLoS One. 2011;6(8):e20988. doi: 10.1371/journal.pone.0020988. Epub 2011 Aug 2.
PMID 21829596
The Tre2 (USP6) oncogene is a hominoid-specific gene.
Paulding CA, Ruvolo M, Haber DA.
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2507-11. Epub 2003 Feb 25.
PMID 12604796
System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B.
Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570.
PMID 21406692
Comparative gene expression profiling reveals partially overlapping but distinct genomic actions of different antiestrogens in human breast cancer cells.
Scafoglio C, Ambrosino C, Cicatiello L, Altucci L, Ardovino M, Bontempo P, Medici N, Molinari AM, Nebbioso A, Facchiano A, Calogero RA, Elkon R, Menini N, Ponzone R, Biglia N, Sismondi P, De Bortoli M, Weisz A.
J Cell Biochem. 2006 Aug 1;98(5):1163-84.
PMID 16514628
Structural analysis of the genome of breast cancer cell line ZR-75-30 identifies twelve expressed fusion genes.
Schulte I, Batty EM, Pole JC, Blood KA, Mo S, Cooke SL, Ng C, Howe KL, Chin SF, Brenton JD, Caldas C, Howarth KD, Edwards PA.
BMC Genomics. 2012 Dec 22;13:719. doi: 10.1186/1471-2164-13-719.
PMID 23260012
The 17q23 amplicon and breast cancer.
Sinclair CS, Rowley M, Naderi A, Couch FJ.
Breast Cancer Res Treat. 2003 Apr;78(3):313-22. (REVIEW)
PMID 12755490
Copy number alterations that predict metastatic capability of human breast cancer.
Zhang Y, Martens JW, Yu JX, Jiang J, Sieuwerts AM, Smid M, Klijn JG, Wang Y, Foekens JA.
Cancer Res. 2009 May 1;69(9):3795-801. doi: 10.1158/0008-5472.CAN-08-4596. Epub 2009 Mar 31.
PMID 19336569


This paper should be referenced as such :
Sapmaz, A ; Erson-Bensan, AE
USP32 (ubiquitin specific peptidase 32)
Atlas Genet Cytogenet Oncol Haematol. 2014;18(1):46-49.
Free journal version : [ pdf ]   [ DOI ]

External links


HGNC (Hugo)USP32   19143
Atlas Explorer : (Salamanque)USP32
Entrez_Gene (NCBI)USP32    ubiquitin specific peptidase 32
AliasesNY-REN-60; USP10
GeneCards (Weizmann)USP32
Ensembl hg19 (Hinxton)ENSG00000170832 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000170832 [Gene_View]  ENSG00000170832 [Sequence]  chr17:60177328-60392133 [Contig_View]  USP32 [Vega]
ICGC DataPortalENSG00000170832
TCGA cBioPortalUSP32
AceView (NCBI)USP32
Genatlas (Paris)USP32
SOURCE (Princeton)USP32
Genetics Home Reference (NIH)USP32
Genomic and cartography
GoldenPath hg38 (UCSC)USP32  -     chr17:60177328-60392133 -  17q23.1-q23.2   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)USP32  -     17q23.1-q23.2   [Description]    (hg19-Feb_2009)
GoldenPathUSP32 - 17q23.1-q23.2 [CytoView hg19]  USP32 - 17q23.1-q23.2 [CytoView hg38]
Genome Data Viewer NCBIUSP32 [Mapview hg19]  
Gene and transcription
Genbank (Entrez)AF155116 AF350251 AF533230 AK057301 AK091680
RefSeq transcript (Entrez)NM_032582
Consensus coding sequences : CCDS (NCBI)USP32
Gene ExpressionUSP32 [ NCBI-GEO ]   USP32 [ EBI - ARRAY_EXPRESS ]   USP32 [ SEEK ]   USP32 [ MEM ]
Gene Expression Viewer (FireBrowse)USP32 [ Firebrowse - Broad ]
GenevisibleExpression of USP32 in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)84669
GTEX Portal (Tissue expression)USP32
Human Protein AtlasENSG00000170832-USP32 [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ8NFA0   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtQ8NFA0  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProQ8NFA0
Catalytic activity : Enzyme3.4.19.12 [ Enzyme-Expasy ] [ IntEnz-EBI ] [ BRENDA ] [ KEGG ]   [ MEROPS ]
Domaine pattern : Prosite (Expaxy)DUSP (PS51283)    EF_HAND_1 (PS00018)    EF_HAND_2 (PS50222)    USP_1 (PS00972)    USP_2 (PS00973)    USP_3 (PS50235)   
Domains : Interpro (EBI)DUSP-like_sf    EF-hand-dom_pair    EF_Hand_1_Ca_BS    EF_hand_dom    Papain-like_cys_pep_sf    Pept_C19_DUSP    Peptidase_C19_UCH    USP_CS    USP_dom   
Domain families : Pfam (Sanger)DUSP (PF06337)    EF-hand_5 (PF13202)    UCH (PF00443)   
Domain families : Pfam (NCBI)pfam06337    pfam13202    pfam00443   
Domain families : Smart (EMBL)DUSP (SM00695)  EFh (SM00054)  
Conserved Domain (NCBI)USP32
AlphaFold pdb e-kbQ8NFA0   
Human Protein Atlas [tissue]ENSG00000170832-USP32 [tissue]
Protein Interaction databases
IntAct (EBI)Q8NFA0
Ontologies - Pathways
Ontology : AmiGOthiol-dependent deubiquitinase  thiol-dependent deubiquitinase  calcium ion binding  protein binding  Golgi apparatus  Golgi apparatus  cytosol  ubiquitin-dependent protein catabolic process  membrane  protein deubiquitination  
Ontology : EGO-EBIthiol-dependent deubiquitinase  thiol-dependent deubiquitinase  calcium ion binding  protein binding  Golgi apparatus  Golgi apparatus  cytosol  ubiquitin-dependent protein catabolic process  membrane  protein deubiquitination  
NDEx NetworkUSP32
Atlas of Cancer Signalling NetworkUSP32
Wikipedia pathwaysUSP32
Orthology - Evolution
GeneTree (enSembl)ENSG00000170832
Phylogenetic Trees/Animal Genes : TreeFamUSP32
Homologs : HomoloGeneUSP32
Homology/Alignments : Family Browser (UCSC)USP32
Gene fusions - Rearrangements
Fusion : MitelmanAATK::USP32 [17q25.3/17q23.1]  
Fusion : MitelmanCDC27::USP32 [17q21.32/17q23.1]  
Fusion : MitelmanCYB561::USP32 [17q23.3/17q23.1]  
Fusion : MitelmanSLC25A10::USP32 [17q25.3/17q23.1]  
Fusion : MitelmanTUBD1::USP32 [17q23.1/17q23.1]  
Fusion : MitelmanUSP32::APPBP2 [17q23.1/17q23.2]  
Fusion : MitelmanUSP32::ARHGAP39 [17q23.1/8q24.3]  
Fusion : MitelmanUSP32::BCAS3 [17q23.1/17q23.2]  
Fusion : MitelmanUSP32::CLTC [17q23.1/17q23.1]  
Fusion : MitelmanUSP32::CRYBA4 [17q23.1/22q12.1]  
Fusion : MitelmanUSP32::CWC25 [17q23.1/17q12]  
Fusion : MitelmanUSP32::DHX40 [17q23.1/17q23.1]  
Fusion : MitelmanUSP32::ELOF1 [17q23.1/19p13.2]  
Fusion : MitelmanUSP32::EPHA1 [17q23.1/7q34]  
Fusion : MitelmanUSP32::GPR137 [17q23.1/11q13.1]  
Fusion : MitelmanUSP32::MAP3K3 [17q23.1/17q23.3]  
Fusion : MitelmanUSP32::MED1 [17q23.1/17q12]  
Fusion : MitelmanUSP32::PACSIN2 [17q23.1/22q13.2]  
Fusion : MitelmanUSP32::PCTP [17q23.1/17q22]  
Fusion : MitelmanUSP32::RAD51C [17q23.1/17q22]  
Fusion : MitelmanUSP32::UBE2O [17q23.1/17q25.1]  
Fusion : MitelmanWIPF2::USP32 [17q21.1/17q23.1]  
Fusion : FusionHubAC098850.4--USP32    AC098850.5--USP32    AC107983.2--USP32    AK295707--USP32    ALG8--USP32    ATP10D--USP32    CACHD1--USP32    CCDC144A--USP32    CCDC49--USP32    CDC27--USP32   
CLTC--USP32    CWC25--USP32    CYB561--USP32    ENSG00000247508--USP32    EPHA1--USP32    FAM222B--USP32    LASS6--USP32    LPXN--USP32    MARCH10--USP32    MED1--USP32   
NPEPPS--USP32    RARA--USP32    RP11-219A15.1--USP32    SENP7--USP32    SLC25A10--USP32    SLC38A10--USP32    SMARCC1--USP32    SUZ12--USP32    TBC1D3--USP32    THRA--USP32   
TRIM22--USP32    TUBD1--USP32    USP32--ABCD3    USP32--AKAP11    USP32--APPBP2    USP32--ARHGAP39    USP32--BCAS3    USP32--C3ORF35    USP32--CCDC49    USP32--CD163L1   
USP32--CDK12    USP32--CIR1    USP32--CLTC    USP32--CREBBP    USP32--CRYBA4    USP32--DDX42    USP32--DHRS3    USP32--DHX40    USP32--ELOF1    USP32--EPHA1   
USP32--EXOSC5    USP32--GCA    USP32--GPR137    USP32--IKZF3    USP32--LINC00511    USP32--MAP2K6    USP32--MAP3K3    USP32--MGEA5    USP32--MKRN1    USP32--MOBP   
USP32--NLK    USP32--PAWR    USP32--PCTP    USP32--PDHA1    USP32--PGS1    USP32--PTGES3    USP32--RAD51C    USP32--RANBP10    USP32--RBFOX3    USP32--RN7SL606P   
USP32--SKAP1    USP32--SPANXN4    USP32--STT3B    USP32--TBC1D16    USP32--TBXAS1    USP32--TTC3    USP32--UBE2O    USP32--UBN2    USP32--UPF2    USP32--USP32   
USP32--USP32P1    USP32--USP6    USP32--VMP1    USP32--WDR13    USP32--ZMYND8    UTP18--USP32    WIPF2--USP32   
Fusion : QuiverUSP32
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerUSP32 [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)USP32
Exome Variant ServerUSP32
GNOMAD BrowserENSG00000170832
Varsome BrowserUSP32
ACMGUSP32 variants
Genomic Variants (DGV)USP32 [DGVbeta]
DECIPHERUSP32 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisUSP32 
ICGC Data PortalUSP32 
TCGA Data PortalUSP32 
Broad Tumor PortalUSP32
OASIS PortalUSP32 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICUSP32  [overview]  [genome browser]  [tissue]  [distribution]  
Somatic Mutations in Cancer : COSMIC3DUSP32
Mutations and Diseases : HGMDUSP32
LOVD (Leiden Open Variation Database)[gene] [transcripts] [variants]
DgiDB (Drug Gene Interaction Database)USP32
DoCM (Curated mutations)USP32
CIViC (Clinical Interpretations of Variants in Cancer)USP32
Impact of mutations[PolyPhen2] [Provean] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
Genetic Testing Registry USP32
NextProtQ8NFA0 [Medical]
Target ValidationUSP32
Huge Navigator USP32 [HugePedia]
Clinical trials, drugs, therapy
Protein Interactions : CTDUSP32
Pharm GKB GenePA134982542
Clinical trialUSP32
DataMed IndexUSP32
PubMed33 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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