Atlas of Genetics and Cytogenetics in Oncology and Haematology
| CASE REPORTS in HAEMATOLOGY |
| Translocation t(2;19)(p11;p12-p13) in childhood with acute myeloid leukemia |
| Benoit Quilichini, Helene Zattara, Elodie Cas, Laure-Anne Bastide-Alliez, Annie Blachere, Catherine Curtillet, Chantal Fossat, Gérard Michel |
| Clinics |
| Age and sex : | 11 year(s) old female patient. |
| Organomegaly : | no hepatomegaly ; no splenomegaly ; no enlarged lymph nodes ; no central nervous system involvement |
| Blood |
| WBC : 194 x 109/l; Hb : 8.6 g/dl; platelets : 17 x 109/l; blasts : 66% |
| Cyto pathology classification |
| Cytology : Blood: neutrophils: 17 %; eosinophils: 1%; basophils: 0%; lymphocytes: 9%; monocytes: 1% ; neutrophils myelocytes- metamyelocytes: 6%; blasts with Auer rods and myelodysplastic features. |
| Immunophenotype : Blasts were myeloperoxydase positive, butyrate acetate negative expressing CD 34, CD 33, CD 13, CD 64, CD 7 and HLA DR. |
| Precise diagnosis : Diagnosis of acute myeloid leukemia with multilineage dysplasia (WHO classification) subtype LAM- M2 (FAB classification). |
| Survival |
| Date of diagnosis: 10-2002 |
| Treatment : Induction treatment : Aracytine : 200 mg/m2/j x 7 days, Novantrone : 12 mg/m2/j x 5 days. |
| Complete remission : None |
| Relapse : - |
| Status : Alive |
| Survival : 7 month(s) |
| Karyotype |
| Sample : Blood and bone marrow. ; culture time : Overnight unstimulated culture and 72h stimulated culture with mitogen. ; banding : R-banding |
| Results : 46, XX, t(2;19)(p11;p12-13) [30] / 46, XX [10] |
| Other molecular cytogenetics technics : Fluorescence in situ hybridization (FISH) was performed using a chromosome 2-specific labelled FITC and a chromosome 19-specific labelled Spectrum Orange painting probes (Adgenix, USA) according to the manufacturer's instructions. |
| Other molecular cytogenetics results : FISH confirmed the transocation t(2,19) |
| Other molecular studies |
| results : ETO / AML1: negative No rearrangment of MLL gene. |
| Other findings |
| results : Meningeal punction without blast cells. |
 |
| Blasts cells (MGG-stained) with Auer rod and degranulated neutrophil (myelodysplastic features) (x100). |
 |
| Bone marrow (MGG-stained) (x50). |
 |
| Karyotype (R-bands) : 46, XX, t(2;19)(p11;p12-13) |
 |
| Fluorescence in situ hybridization using painting probes of chromosome 2 labelled FITC (WCP 2) and chromosome 19 labelled Spectrum Orange (WCP 19) (ADgenix, USA). |
| Comments |
| We report a translocation t(2;19)(p11;p12-13) occuring in a childhood acute myeloid leukemia, subtype M2. Cytological particularity of this case was the presence of myelodysplastic signs. Rearrangement of 19p13 is a common feature in preB-ALL showing t(1;19)(q23;p13) but not in AML. The t(1;19) involves in 19p13 E2A gene which normally encodes an immunoglobulin enhancer binding proteins. One case was reported showing t(2 ;19) in acute leukemia. The case was an AML secondary to chemotherapy for ovarian cancer. Karyotype showed complex abnormalities including a t(2 ;19)(p11 ;p13) [2]. Variability of the breakpoints at 2p and 19q in our patient, compared with the case described in literature, could be due to the fact that the authors used G banding techniques. |
| Internal links |
| Atlas Card | t(2;19)(p11;p12-p13) |
| Bibliography |
| Proposed revised criteria for the classification of acute myeloid leukemia. A report of the French-American-British Cooperative Group. |
| Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR, Sultan C. |
| Ann Intern Med 1985; 103: 620-625. |
| PMID 3862359 |
| Short remission durations in therapy-related leukemia despite cytogenetic complete responses to hight-dose cytarabine. |
| Larson RA, Wernli M, Le Beau MM, Daly KM, Pape LH, Rowley JD, Vardiman JW. |
| Blood 1988; 72(4): 1333-1339. |
| PMID 3167210 |
| World health organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues : report of the clinical advisory committee meeting - Airlie House, Virginia, november 1997. |
| Harris NL, Jaffe EF, Diebold J, Flandrin G, Muller Hermelink HK, Vardiman J, Lister TA, Bloomfield CD. |
| Journal of clinical oncology 1999; 17(12): 3835-3849. |
| PMID 10577857 |
| WEB : Atlas of Genetics and Cytogenetics in Oncology and Haematology |
| The Cancer Genome Anatomy Project - Mitelman Cases Quick Searcher |
| Contributor(s) |
| Written | 12-2002 | Benoit Quilichini, Helene Zattara, Elodie Cas, Laure-Anne Bastide-Alliez, Annie Blachere, Catherine Curtillet, Chantal Fossat, Gérard Michel |
| Citation |
| This paper should be referenced as such : |
| Quilichini B, Zattara H, Cas E, Bastide-Alliez LA, Blachere A, Curtillet C, Fossat C, Michel G . Translocation t(2;19)(p11;p12-p13) in childhood with acute myeloid leukemia. Atlas Genet Cytogenet Oncol Haematol. December 2002 . URL : http://AtlasGeneticsOncology.org/Reports/0219QuilichiniID100006.html |
| © Atlas of Genetics and Cytogenetics in Oncology and Haematology | indexed on : Thu Apr 17 14:15:19 2008 |
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