| Written | 2007-04 | Clelia Tiziana Storlazzi, Fredrik Mertens |
| Department of Genetics, Microbiology, University of Bari, Via Amendola 165/A, 70126 Bari, Italy (CTS);, Dept of Clinical Genetics, Lund University Hospital, SE-221 85 Lund, Sweden (FM). |
| Identity |
| ICD-Topo | C470-C476,C478-C479,C490-C496,C498-C499 CONNECTIVE & SOFT TISSUE |
| ICD-Morpho | 9040/3 Synovial sarcoma, NOS |
| Atlas_Id | 5464 |
| Phylum | Soft Tissues::Synovial sarcoma |
| Clinics and Pathology |
| Disease | Biphasic Synovial Sarcoma |
| Pathology | The excised tumor consisted of a single 3-cm nodulus with relatively well-defined borders and a grey cut surface. No necrosis was seen. In histological sections stained with H and E, the tumor was mainly composed of uniform, closely packed spindle cells, with a high nuclear/cytoplasmic ratio and finely dispersed chromatin. The tumor cells were arranged in fascicles and whorls, with small foci of calcification. The mitotic activity was low. In addition, small and irregular glandular formations lined by flattened cuboidal cells were noted in the peripheral parts of the tumor, supporting the diagnosis of biphasic synovial sarcoma. These cells, as well as many spindle cells, expressed cytokeratins and epithelial membrane antigen (EMA). |
| Treatment | The patient was given postoperative chemo- and radiotherapy. |
| Evolution | Four months postoperatively, the patient is alive without any sign of local recurrence or metastatic disease. |
| Cytogenetics |
| Cytogenetics Morphological | 48,XY,+mar1,+mar2[5]/47,idem,dic(19;22)(q13;q13)[3] . |
 | |
| Top: Partial karyotype showing G-banded chromosomes X, 18, 20 and markers 1 and 2. Bottom: FISH cohybridization using a pool of RP11-552E4 and RP11-344N17 (red), RP5-1005F21 (purple), and pZ20 (green) as probes for chromosomes X, 20, and the two markers. The results on mar2 are shown as a three-color image (left), as well as separately for each of the probes (right). | |
| Cytogenetics Molecular | With a pool of SSX BAC probes, one and two signals were seen on mar1 and mar2, respectively. On the latter marker chromosome, one of the signals co-localized with the signal from RP5-1005F21, suggesting the presence of a fusion gene between SS18L1 and one of the SSX genes. |
| Probes | RP5-1005F21 (SS18L1), RP11-552E4 and RP11-344N17 (pool of SSX genes). |
| Genes involved and Proteins |
| Gene Name | SS18L1 |
| Location | 20q13.33 |
| Note | Paralogous gene to SS18 (18q11.2). |
| Dna / Rna | Genomic (chr20:60,169,869-60,189,352). Transcript of 4566 bp (NM_198935). |
| Gene Name | SSX1 |
| Location | Xp11.23 |
| Note | Gene belonging to the SSX gene family. |
| Dna / Rna | Genomic (chrX:47,999,741-48,011,823). Transcript of 1271 bp (NM_005635). |
| Protein | 188 amino acids (Q16384). |
| Result of the chromosomal anomaly |
| Hybrid Gene | |
| Description | Nucleotide 1216 (exon 10) of SS18L1 (Accession Number XM_037202) was fused in-frame with nt 422 (exon 6) of SSX1 (Accession Number X86174). |
| Fusion Protein | |
| Description | In the putative SS18L1/SSX1 chimeric protein, the last 8 amino acid residues of the SS18L1 protein are replaced by 78 amino acids from the COOH-terminal part of SSX1. By analogy with what is presumed to be the case for the SS18/SSX fusion protein, SS18L1/SSX1 is likely to show an altered transcriptional pattern, with the COOH-terminal SSX domain redirecting the SS18L1 activation domain to new target promoters. |
| Bibliography |
| A novel fusion gene, SS18L1/SSX1, in synovial sarcoma. |
| Storlazzi CT, Mertens F, Mandahl N, Gisselsson D, Isaksson M, Gustafson P, Domanski HA, Panagopoulos I |
| Genes, chromosomes & cancer. 2003 ; 37 (2) : 195-200. |
| PMID 12696068 |
| Citation |
| This paper should be referenced as such : |
| Storlazzi, Clelia T |
| Soft Tissue Tumors: t(X;20)(p11.23;q13.33) in Biphasic Synovial Sarcoma |
| Atlas Genet Cytogenet Oncol Haematol. 2007;11(4):340-339. |
| Free journal version : [ pdf ] [ DOI ] |
| On line version : http://AtlasGeneticsOncology.org/Tumors/SynovialSarcomtX20ID5464.html |
| Translocations implicated (Data extracted from papers in the Atlas) |
| t(X;20)(p11;q13) SS18L1/SSX1 | |
| External links |
| REVIEW articles | automatic search in PubMed |
| Last year articles | automatic search in PubMed |
| © Atlas of Genetics and Cytogenetics in Oncology and Haematology | indexed on : Fri Jun 30 11:25:12 CEST 2017 |
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