Translocation t(2;19)(p11;p12-p13) in childhood with acute myeloid leukemia

Benoit Quilichini, Helene Zattara, Elodie Cas, Laure-Anne Bastide-Alliez, Annie Blachere, Catherine Curtillet, Chantal Fossat, Gérard Michel  

Cytogenetic Oncology Laboratory Timone 264, rue Saint-Pierre 13385 Marseille cedex 5, France; Tel : +33-491387402 - Fax : +33-491384663 e-mail : Helene.Cannoni@mail.ap-hm.fr, Benoit.Quilichini@mail.ap-hm.fr

Previous history

Preleukaemia
No preleukemia.
Malignant disease
No previous solid tumors.

Clinics case report

Age
11 yrs
Sex
F
Liver
-
Spleen
-
Lymph nodes
-
Cns involv
-

Blood data

Wbc
194
Hb
8.6
Platelets
17
Blasts
66
Bone marrow
Granulopoiesis with myelodysplastic features.

Cyto path

Cytology
Blood: neutrophils: 17 %; eosinophils: 1%; basophils: 0%; lymphocytes: 9%; monocytes: 1% ; neutrophils myelocytes- metamyelocytes: 6%; blasts with Auer rods and myelodysplastic features.
Immunophenotype
Blasts were myeloperoxydase positive, butyrate acetate negative expressing CD 34, CD 33, CD 13, CD 64, CD 7 and HLA DR.
Precise diagnosis
Diagnosis of acute myeloid leukemia with multilineage dysplasia (WHO classification) subtype LAM- M2 (FAB classification).

Survival data

Date diagnosis
10-2002
Treatment
Induction treatment : Aracytine : 200 mg/m2/j x 7 days, Novantrone : 12 mg/m2/j x 5 days.
Complete remission
After induction (J30)
Relapse
-
Status
A
Survival
7 +

Karyotype

Sample
Blood and bone marrow.
Culture time
Overnight unstimulated culture and 72h stimulated culture with mitogen.
Banding
R-banding
Results
46, XX, t(2;19)(p11;p12-13) [30] / 46, XX [10]
Mol cytogenet technics
Fluorescence in situ hybridization (FISH) was performed using a chromosome 2-specific labelled FITC and a chromosome 19-specific labelled Spectrum Orange painting probes (Adgenix, USA) according to the manufacturer s instructions.
Mol cytogenet results
FISH confirmed the transocation t(2,19)

Other molec studies

Results
No rearrangment of gene.

Other findings

Note
Meningeal punction without blast cells.

Images

Atlas Image
Blasts cells (MGG-stained) with Auer rod and degranulated neutrophil (myelodysplastic features) (x100).
Atlas Image
Bone marrow (MGG-stained) (x50).
Atlas Image
Karyotype (R-bands) : 46, XX, t(2;19)(p11;p12-13)
Atlas Image
Fluorescence in situ hybridization using painting probes of chromosome 2 labelled FITC (WCP 2) and chromosome 19 labelled Spectrum Orange (WCP 19) (ADgenix, USA).

Comments section

Comments
We report a translocation t(2;19)(p11;p12-13) occuring in a childhood acute myeloid leukemia, subtype M2. Cytological particularity of this case was the presence of myelodysplastic signs. Rearrangement of 19p13 is a common feature in preB-ALL showing but not in AML. The t(1;19) involves in 19p13 gene which normally encodes an immunoglobulin enhancer binding proteins. One case was reported showing t(2 ;19) in acute leukemia. The case was an AML secondary to chemotherapy for ovarian cancer. Karyotype showed complex abnormalities including a t(2 ;19)(p11 ;p13) [2]. Variability of the breakpoints at 2p and 19q in our patient, compared with the case described in literature, could be due to the fact that the authors used G banding techniques.

Bibliography

Pubmed IDLast YearTitleAuthors
38623591985Proposed revised criteria for the classification of acute myeloid leukemia. A report of the French-American-British Cooperative Group.Bennett JM et al
31672101988Short remission durations in therapy-related leukemia despite cytogenetic complete responses to high-dose cytarabine.Larson RA et al
105778571999World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997.Harris NL et al

Citation

Benoit Quilichini, Helene Zattara, Elodie Cas, Laure-Anne Bastide-Alliez, Annie Blachere, Catherine Curtillet, Chantal Fossat, Gérard Michel

Translocation t(2;19)(p11;p12-p13) in childhood with acute myeloid leukemia

Atlas Genet Cytogenet Oncol Haematol. 2002-12-01

Online version: http://atlasgeneticsoncology.org/case-report/208804/translocation-t(2;19)(p11;p12-p13)-in-childhood-with-acute-myeloid-leukemia