A new case of translocation t(14;14)(q11;q32) in B lineage ALL

Elvira D Rodrigues Pereira Velloso, Priscila Pereira dos Santos Teixeira, Karina Prandi Melillo, Luciana J Rodrigues da Silva, Cristina Alonso Ratis, Daniela Borri, Cristóvão LP Mangueira  

Clinical Laboratory, Hospital Israelita Albert Einstein, Sao Paulo, Brazil (EDRPV, PPdST, LJRdS, CAR, DB, CLPM); CASE Intermedica, Sao Paulo, Brazil (KPM)

Previous history

Preleukaemia
-
Malignant disease
-
Inborn condition
-

Clinics case report

Age
43 yrs
Sex
M
Liver
+
Spleen
+
Lymph nodes
-
Cns involv
+

Blood data

Wbc
227
Hb
5,9
Platelets
51
Blasts
12
Bone marrow
aspirate: 90% lymphoblast

Cyto path

Cytology
LLA-L2
Immunophenotype
HLA-DR+, TdT+, CD79a+, CD19+, cyCD22+, CD20+, CD10+
Rearranged ig tcr
rearranged IGH (FISH)
Pathology
not done
Electron microscopy
not done
Precise diagnosis
common B-ALL

Survival data

Date diagnosis
02-2009
Treatment
Cancer and Leukemia Group B (CALGB) protocol
Complete remission
not evaluated
Treatment relat death
+ Neutropenia and lung infection
Relapse
-
Status
D
Date last follow
03-2009
Survival
20 days

Karyotype

Sample
Bone marrow
Culture time
24 and 48 hours without stimulating agents
Banding
G
Results
46,XY,t(14;14)(q11;q32.1)[20]
Karyotype relapse
not done
Mol cytogenet technics
FISH using IGH Break Apart Rearrangement Probe, Vysis
Mol cytogenet results
nuc ish(IGHx2)(5 IGH sep 3 IGHx1)[154/200]/ (5 IGHx2,3 IGHx1)(5 IGH con 3 IGHx1)[33/200]

Images

Atlas Image
G- banded partial karyotypes showing the t(14;14).
Atlas Image
Interphase FISH shows IGH gene rearrangements (IGH Dual Color, Break Apart Rearrangement probe).

Comments section

Comments
The t(14;14)(q11;q32) CEBPE/IGH may be associated with good prognosis in B-ALL. In 4 cases with clinical follow-up, complete remission was achieved and those were alive at the time of report. In the case described herein, the t(14;14) was the sole anomaly, IGH rearrangement was detected but CEBPE involvement was not studied. This patient has well known bad prognostic features as high WBC count and CNS involvement and died few days after diagnosis.

Bibliography

Pubmed IDLast YearTitleAuthors
152624342004IGH gene involvement in two cases of acute lymphoblastic leukemia with t(14;14)(q11;q32) identified by sequential R-banding and fluorescence in situ hybridization.Liu S et al
171701242007Five members of the CEBP transcription factor family are targeted by recurrent IGH translocations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL).Akasaka T et al
190274932008Translocation (14;14)(q11;q32) with simultaneous involvement of the IGH and CEBPE genes in B-lineage acute lymphoblastic leukemia.Han Y et al

Citation

Elvira D Rodrigues Pereira Velloso, Priscila Pereira dos Santos Teixeira, Karina Prandi Melillo, Luciana J Rodrigues da Silva, Cristina Alonso Ratis, Daniela Borri, Cristóvão LP Mangueira

A new case of translocation t(14;14)(q11;q32) in B lineage ALL

Atlas Genet Cytogenet Oncol Haematol. 2011-02-01

Online version: http://atlasgeneticsoncology.org/case-report/208846/a-new-case-of-translocation-t(14;14)(q11;q32)-in-b-lineage-all