+6 or trisomy 6

2004-06-01   Edmond SK Ma , Thomas SK Wan 

1.Hematology Division, Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, P.R. China

Clinics and Pathology

Note

Trisomy 6 (+6) has been reported as the sole cytogenetics aberration in 14 cases of acute myeloid leukaemia (AML) and five cases of myelodysplastic syndrome. Three out of four AML patients with +6 in one series showed AML-M1 morphology and expression of stem cell antigen CD34 on the leukaemic blasts, suggesting that +6 may be associated with a more primitive form of AML. Nevertheless, other reported cases represent a broader spectrum of AML and it is therefore premature to draw conclusion as to whether special morphological and phenotypic features exist in AML with +6.

Intriguingly, +6 as the sole cytogenetics abnormality have been reported in 12 cases of haematological disorders characterized by peripheral blood cytopenia and hypoplastic bone marrow. Among the 12 cases, eight cases showed dysplastic change in the haemopoietic cells, whereas four did not. In two cases of aplastic anaemia with dyserythropoiesis and +6, FISH showed +6 in erythroid as well as myeloid cells, suggesting involvement of an early haemopoietic progenitor cell. The overall survival ranges from 9 - 48 months. In all cases, normal cells without +6 are present, and the percentage of metaphases with +6 ranges from 6.9% to 85%. It is questionable whether such cases should be classified as hypoplastic MDS, based on clonal cytogenetic change of +6, or aplastic anaemia. The pathogenesis of aplastic anaemia is heterogeneous. While an immunological basis for this disorder is established based on response to immunosuppressive therapy, there is also evidence for clonal nature resulting from damage to the haemopoietic stem cell compartment. Indeed clonal chromosomal abnormalities are reported in otherwise typical aplastic anaemia. Furthermore, aplastic anaemia evolving into acute leukaemia is well documented. This is exemplified by the development of AML in a case of aplastic anaemia with +6 and no dysplasia 15 months after initial diagnosis. Although patients with +6 and marrow aplasia were uniformly non-responsive to treatments by steroids and anti-thymocyte globulin (ATG), clinical response to cyclosporine was seen in two cases. There was improvement of platelet count in one case and complete clinical response in another. Taken together, it is plausible that +6 defines a distinctive subtype of aplastic anaemia with mild dysplastic changes, poor response to steroids and ATG therapy, and a propensity for AML transformation.

Rare instances of +6 may be encountered in childhood acute mixed lineage leukaemia, lymphoblastic transformation of chronic myeloid leukaemia, and chronic myeloproliferative disorder.

Phenotype stem cell origin

Three out of four AML patients with +6 in one series showed AML-M1 morphology and expression of stem cell antigen CD34 on the leukaemic blasts, suggesting that +6 may be associated with a more primitive form of AML.

Evolution

A case of aplastic anaemia with +6 and no dysplasia showed transformation into AML at 15 months after initial diagnosis.

Prognosis

Trisomy 6 may define a distinctive subtype of aplastic anaemia with mild dysplastic changes, poor response to steroids and ATG therapy, and a propensity for AML transformation. More cases need to be collected to substantiate this contention.

Cytogenetics

Atlas Image
Complete karyotype showing 47,XY,+6. G-banding with trypsin/Giemsa. The patient is an 81-year old Chinese man who was admitted for myocardial infarction and was found to be anaemic. Physical examination was unremarkable. Blood counts showed: haemoglobin 8.6 g/dL, white cell count 3.2 X 109/L, and platelet count 174 X 109/L. Bone marrow morphology, cytochemistry and immunophenotyping results were consistent with a diagnosis of acute myeloid leukaemia. Chromosome analysis on bone marrow cells showed: 47,XY,+6[2]/46,XY[5].

Bibliography

Pubmed IDLast YearTitleAuthors
14057151992Primary, single, autosomal trisomies associated with haematological disorders. United Kingdom Cancer Cytogenetics Group (UKCCG).
33157241987Clonal cytogenetic abnormalities in patients with otherwise typical aplastic anemia.Appelbaum FR et al
2894241979Correlation between prognosis and bone marrow chromosomal patterns in children with acute nonlymphocytic leukemia: similarities and differences compared to adults.Benedict WF et al
13941021992Cytogenetic analysis of hematologic malignancies in Hong Kong. A study of 98 cases.Chan LC et al
10021601976Trisomy 6 associated with aplastic anemia.Geraedts JP et al
80199611994Trisomy 6 as the sole chromosome abnormality in myeloid disorders.Jonveaux P et al
96899311998Trisomy 6 is the hallmark of a dysplastic clone in bone marrow aplasia.La Starza R et al
38013191986Multiple chromosomally distinct cell populations in myelodysplastic syndromes and their possible significance in the evolution of the disease.Mecucci C et al
97977811998Trisomy 6 as a primary karyotypic aberration in hematologic disorders.Mohamed AN et al
20014621991Trisomy 6: a recurring cytogenetic abnormality associated with marrow hypoplasia.Moormeier JA et al
98933021998Trisomy 6 in a childhood acute mixed lineage leukemia.Onodera N et al
69295651980Chromosome aberrations and prognosis in preleukaemia.Panani A et al
97977881998Trisomy 6 and double minute chromosomes in a case of chronic myelomonocytic leukemia.Sambani C et al
38552851985Chromosomal alterations in acute leukemia patients studied with improved culture methods.Testa JR et al
34221671988Prognostic significance of chromosome analysis in de novo acute myeloid leukemia (AML).Weh HJ et al
150368972004A rapidly progressive chronic myeloproliferative disease with isolated trisomy 6.Wong KF et al
128854702003Trisomy 6 acquired in lymphoid blast transformation of chronic myelocytic leukemia with t(9;22).Yilmaz Y et al

Citation

Edmond SK Ma ; Thomas SK Wan

+6 or trisomy 6

Atlas Genet Cytogenet Oncol Haematol. 2004-06-01

Online version: http://atlasgeneticsoncology.org/haematological/1013/+6-or-trisomy-6