A new case of t(8;14)(q11;q32) in an acute lymphoblastic leukemia

Benoit Quilichini, Helene Zattara, Franois Casalonga, Laure-Anne Bastide-Alliez, Catherine Curtillet, Chantal Fossat, Gérard Michel  

Cytogenetic Oncology Laboratory : CHU Timone 264, rue Saint-Pierre 13385 Marseille cedex 5, France ; Tel : +33-491387402 ï Fax : +33-491384663 e-mail : Helene.Cannoni@mail.ap-hm.fr, Benoit.Quilichini@mail.ap-hm.fr

Previous history

Preleukaemia
-
Malignant disease
-
Inborn condition
-

Clinics case report

Age
13 yrs
Sex
M
Liver
-
Spleen
-
Lymph nodes
-
Cns involv
-

Blood data

Wbc
21
Hb
: 9.1
Platelets
32
Blasts
79
Bone marrow
97

Cyto path

Cytology
neutrophils : 11 %; eosinophils : 0 %; basophils : 0%; lymphocytes : 9 %; monocytes : 1%
Immunophenotype
HLA DR+ (high), CD34+ (high), CD10+ (low), CD19+ (high), c mu -, CD79a+ (high). Blasts were myeloperoxydase negative.
Precise diagnosis
Diagnosis of precursor B-cell acute lymphoblastic leukemia (WHO classification), LAL-B II (EGIL classification).

Survival data

Date diagnosis
04-2003
Complete remission
+

Karyotype

Sample
Blood and bone marrow.
Culture time
Overnight unstimulated culture
Banding
R-BANDING
Results
45, X, - Y, t(8;14)(q11;q32) [ 16 ] / 46, XY [ 4 ]
Mol cytogenet technics
2- FISH was performed using a chimeric X/Y direct labelled coktail probe (Qbiogene) according to the manufacturer s instructions. FISH confirmed the loss of chromosome Y in 90% on interphase cells (data not shown).

Other molec studies

Results
: no rearrangement

Images

Atlas Image
Figure 1: Bone marrow (MGG-stained ) x 50: Increased cellularity, no megakaryocyte. Blasts : 97 % ; Neutrophils : 3%.
Atlas Image
Figure 2: Karyotype ( R-bands) : 45, X, -Y, t(8;14)(q11;q32).
Atlas Image
Figure 3: Fluorescence in situ hybridization using WCP 8 probe ( Spectrum Orange) and LSI-IgH locus probe ( FITC): partial karyotype results ( R-banding and FISH ).

Comments section

Comments
The breakpoint at 8q11 has not yet been cloned.

Bibliography

Pubmed IDLast YearTitleAuthors
75645261995Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL).Bene MC et al
105778571999World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997.Harris NL et al
106737392000Acute lymphoblastic leukemia with an unusual t(8;14)(q11.2;q32): a Pediatric Oncology Group Study.Kaleem Z et al
125818912003Acute lymphoblastic leukemia characterized by t(8;14)(q11.2;q32).Moore S et al

Citation

Benoit Quilichini, Helene Zattara, Franois Casalonga, Laure-Anne Bastide-Alliez, Catherine Curtillet, Chantal Fossat, Gérard Michel

A new case of t(8;14)(q11;q32) in an acute lymphoblastic leukemia

Atlas Genet Cytogenet Oncol Haematol. 2003-05-01

Online version: http://atlasgeneticsoncology.org/case-report/208805/a-new-case-of-t(8;14)(q11;q32)-in-an-acute-lymphoblastic-leukemia