A new case of t(8;14)(q11;q32) in an acute lymphoblastic leukemia

Benoit Quilichini, Helene Zattara, Franois Casalonga, Laure-Anne Bastide-Alliez, Catherine Curtillet, Chantal Fossat, Gérard Michel  

Cytogenetic Oncology Laboratory : CHU Timone 264, rue Saint-Pierre 13385 Marseille cedex 5, France ; Tel : +33-491387402 ï Fax : +33-491384663 e-mail : Helene.Cannoni@mail.ap-hm.fr, Benoit.Quilichini@mail.ap-hm.fr

Previous history

Preleukaemia
-
Malignant disease
-
Inborn condition
-

Clinics case report

Age
13 yrs
Sex
M
Liver
-
Spleen
-
Lymph nodes
-
Cns involv
-

Blood data

Wbc
21
Hb
: 9.1
Platelets
32
Blasts
79
Bone marrow
97

Cyto path

Cytology
neutrophils : 11 %; eosinophils : 0 %; basophils : 0%; lymphocytes : 9 %; monocytes : 1%
Immunophenotype
HLA DR+ (high), CD34+ (high), CD10+ (low), CD19+ (high), c mu -, CD79a+ (high). Blasts were myeloperoxydase negative.
Precise diagnosis
Diagnosis of precursor B-cell acute lymphoblastic leukemia (WHO classification), LAL-B II (EGIL classification).

Survival data

Date diagnosis
04-2003
Complete remission
+

Karyotype

Sample
Blood and bone marrow.
Culture time
Overnight unstimulated culture
Banding
R-BANDING
Results
45, X, - Y, t(8;14)(q11;q32) [ 16 ] / 46, XY [ 4 ]
Mol cytogenet technics
2- FISH was performed using a chimeric X/Y direct labelled coktail probe (Qbiogene) according to the manufacturer s instructions. FISH confirmed the loss of chromosome Y in 90% on interphase cells (data not shown).

Other molec studies

Results
: no rearrangement

Images

Atlas Image
Figure 1: Bone marrow (MGG-stained ) x 50: Increased cellularity, no megakaryocyte. Blasts : 97 % ; Neutrophils : 3%.
Atlas Image
Figure 2: Karyotype ( R-bands) : 45, X, -Y, t(8;14)(q11;q32).
Atlas Image
Figure 3: Fluorescence in situ hybridization using WCP 8 probe ( Spectrum Orange) and LSI-IgH locus probe ( FITC): partial karyotype results ( R-banding and FISH ).

Comments section

Comments
The breakpoint at 8q11 has not yet been cloned.

Bibliography

Pubmed IDLast YearTitleAuthors

Citation

Benoit Quilichini, Helene Zattara, Franois Casalonga, Laure-Anne Bastide-Alliez, Catherine Curtillet, Chantal Fossat, Gérard Michel

A new case of t(8;14)(q11;q32) in an acute lymphoblastic leukemia

Atlas Genet Cytogenet Oncol Haematol. 2003-05-01

Online version: http://atlasgeneticsoncology.org/case-report/208805/gene-fusions-explorer/