RPRM (reprimo, TP53 dependent G2 arrest mediator candidate)
2011-11-01 Alejandro H Corvalan , Veronica A Torres AffiliationLaboratory of Molecular Pathology, Epidemiology, Department of Hemathology - Oncology, School of Medicine - P Universidad Catolica de Chile, 391 Marcoleta St - Santiago 8330074 Chile
DNA/RNA
Description
Reprimo gene consists of 1 exon. The gene spans 1,47 kb of genomic DNA on the chromosome 2 in the minus strand.
Transcription
The mRNA is 1496 bp in length.
Proteins
Description
The open reading frame encodes a 109 amino acid protein with an estimated molecular weight of 11774 Da. Reprimo is a highly glycosylated protein which has two sites in amino acids 7 and 18. The protein has a potential transmembrane site covering amino acids 56 to 76.
Expression
The expression of Reprimo is induced by tumor protein p53 following X-ray irradiation.
Localisation
When Reprimo is ectopically expressed, it is localized in the cytoplasm.
Function
Reprimo is a candidate tumor suppresor gene involved in the G2/M phase cell cycle arrest mediated by tumor protein p53. Reprimo induces cell cycle arrest by inhibiting the nuclear translocation of the Cdc2-Cyclin B1 complex.
Implicated in
Entity name
Various cancers
Note
The aberrant methylation of the promoter region of Reprimo is a common event that may contribute to the pathogenesis of some types of human cancer. Promoter methylation of Reprimo was found in pancreatic cancer (91%), gastric cancer (90%), gallbladder cancer (62%), lymphomas (57%), colorectal cancer (56%) and esophageal adenocarcinomas (40%). In breast cancer, leukemias and lung cancer, promoter methylation of Reprimo was found in less than 40% of tested cases.
Entity name
Gastric cancer
Disease
Aberrant hypermethylation of Reprimo is frequently found in primary gastric cancer as well as in pair plasma samples. In plasma from asymptomatic controls, Reprimo is infrequently methylated. Therefore, plasmatic detection of Reprimo is a putative biomarker for early detection of gastric cancer.
The above histogram represents the percentage of positive cases for Reprimo and other genes (APC, SHP1, CDH-1, ER, SEMA3B and 3OST2) in 43 prospectively collected gastric cancer cases and 31 asymptomatic age- and gender-matched controls. Only Reprimo shows a significant difference in plasma between gastric cancer and asymptomatic controls (Bernal et al., Clin Cancer Res. 2008;14:6264-9).
Entity name
Pancreatic cancer
Disease
Aberrant hypermethylation of Reprimo is also common in pancreatic cell lines (91%) and in pancreatic adenocarcinomas (66%). Reprimo methylation is correlated with poor prognosis in a large series of resected pancreatic cancers. This fact raises the possibility that aberrant methylation of Reprimo is an epigenetic event that may have a mechanistic role in pancreatic cancer.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 18829507 | 2008 | Reprimo as a potential biomarker for early detection in gastric cancer. | Bernal C et al |
| 17121882 | 2006 | Reprimo methylation is a potential biomarker of Barrett's-Associated esophageal neoplastic progression. | Hamilton JP et al |
| 10930422 | 2000 | Reprimo, a new candidate mediator of the p53-mediated cell cycle arrest at the G2 phase. | Ohki R et al |
| 16752411 | 2006 | Aberrant methylation of Reprimo correlates with genetic instability and predicts poor prognosis in pancreatic ductal adenocarcinoma. | Sato N et al |
| 15713514 | 2005 | Aberrant methylation of Reprimo in lung cancer. | Suzuki M et al |
| 15700311 | 2005 | Aberrant methylation of Reprimo in human malignancies. | Takahashi T et al |
Other Information
Locus ID:
NCBI: 56475
MIM: 612171
HGNC: 24201
Ensembl: ENSG00000177519
Variants:
dbSNP: 56475
ClinVar: 56475
TCGA: ENSG00000177519
COSMIC: RPRM
RNA/Proteins
| Gene ID | Transcript ID | Uniprot |
|---|---|---|
| ENSG00000177519 | ENST00000325926 | Q9NS64 |
Expression (GTEx)
Pathways
| Pathway | Source | External ID |
|---|---|---|
| p53 signaling pathway | KEGG | ko04115 |
| p53 signaling pathway | KEGG | hsa04115 |
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 35549646 | 2022 | Downregulated Reprimo by LINC00467 participates in the growth and metastasis of gastric cancer. | 1 |
| 35549646 | 2022 | Downregulated Reprimo by LINC00467 participates in the growth and metastasis of gastric cancer. | 1 |
| 29401170 | 2018 | Effect of Reprimo Down-regulation on Malignant Transformation of Intraductal Papillary Mucinous Neoplasm. | 3 |
| 29941787 | 2018 | The Reprimo Gene Family: A Novel Gene Lineage in Gastric Cancer with Tumor Suppressive Properties. | 12 |
| 29401170 | 2018 | Effect of Reprimo Down-regulation on Malignant Transformation of Intraductal Papillary Mucinous Neoplasm. | 3 |
| 29941787 | 2018 | The Reprimo Gene Family: A Novel Gene Lineage in Gastric Cancer with Tumor Suppressive Properties. | 12 |
| 28562620 | 2017 | Reprimo tissue-specific expression pattern is conserved between zebrafish and human. | 7 |
| 28809778 | 2017 | Reprimo, a Potential p53-Dependent Tumor Suppressor Gene, Is Frequently Hypermethylated in Estrogen Receptor α-Positive Breast Cancer. | 7 |
| 28562620 | 2017 | Reprimo tissue-specific expression pattern is conserved between zebrafish and human. | 7 |
| 28809778 | 2017 | Reprimo, a Potential p53-Dependent Tumor Suppressor Gene, Is Frequently Hypermethylated in Estrogen Receptor α-Positive Breast Cancer. | 7 |
| 27992600 | 2016 | Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer. | 10 |
| 27992600 | 2016 | Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer. | 10 |
| 25629980 | 2015 | tp53-dependent G2 arrest mediator candidate gene, Reprimo, is down-regulated by promoter hypermethylation in pediatric acute myeloid leukemia. | 4 |
| 25954972 | 2015 | Loss of Expression of Reprimo, a p53-induced Cell Cycle Arrest Gene, Correlates with Invasive Stage of Tumor Progression and p73 Expression in Gastric Cancer. | 24 |
| 25954972 | 2015 | Loss of Expression of Reprimo, a p53-induced Cell Cycle Arrest Gene, Correlates with Invasive Stage of Tumor Progression and p73 Expression in Gastric Cancer. | 24 |
Citation
Alejandro H Corvalan ; Veronica A Torres
RPRM (reprimo, TP53 dependent G2 arrest mediator candidate)
Atlas Genet Cytogenet Oncol Haematol. 2011-11-01
Online version: http://atlasgeneticsoncology.org/gene/42082/rprm
