RUVBL2 (RuvB-like 2 (E. coli))
2009-03-01 Aude Grigoletto , Valérie Haurie , Jean Rosenbaum AffiliationINSERM U889, Universite Victor Segalen Bordeaux 2, 146 rue Leo Saignat, 33076 Bordeaux, France
Identity
HGNC
LOCATION
19q13.33
LOCUSID
ALIAS
CGI-46,ECP-51,ECP51,INO80J,REPTIN,RVB2,TAP54-beta,TIH2,TIP48,TIP49B
DNA/RNA
Description
15 exons, 14 introns (Parfait et al., 2000).
Transcription
1518bp mRNA with 463aa open reading frame.
Proteins
Description
463 amino acids, 52 kDa.
RUVBL2 belongs to the AAA+ ATPase superfamily (ATPases associated with diverse cellular activities) sharing conserved Walker A and B motifs, arginine fingers, and sensor domains. The monomers contain two domains, which are involved in ATP binding and hydrolysis respectively. RUVBL2 assembles into an hexameric structure with a central channel.
RUVBL2 interacts with RUVBL1 to form a dodecamer (Puri et al., 2007). This RUVBL1/RUVBL2 complex displays a significant ATPase activity and is likely one of the functional forms of the proteins.
Sumoylation of RUVBL2 has been reported on Lys456 in invasive prostate cancer cells (Kim et al., 2006).
RUVBL2 is phosphorylated on an ATM/ATR consensus site following DNA damage (Matsuoka et al., 2007).
RUVBL2 belongs to the AAA+ ATPase superfamily (ATPases associated with diverse cellular activities) sharing conserved Walker A and B motifs, arginine fingers, and sensor domains. The monomers contain two domains, which are involved in ATP binding and hydrolysis respectively. RUVBL2 assembles into an hexameric structure with a central channel.
RUVBL2 interacts with RUVBL1 to form a dodecamer (Puri et al., 2007). This RUVBL1/RUVBL2 complex displays a significant ATPase activity and is likely one of the functional forms of the proteins.
Sumoylation of RUVBL2 has been reported on Lys456 in invasive prostate cancer cells (Kim et al., 2006).
RUVBL2 is phosphorylated on an ATM/ATR consensus site following DNA damage (Matsuoka et al., 2007).
Expression
Expression of RUVBL2 is ubiquitous but especially abundant in thymus and testis (Salzer et al., 1999; Parfait et al., 2000).
RUVBL2 is overexpressed in hepatocellular carcinoma (Rousseau et al., 2007). Overexpression of RUVBL2 in several cancers and its possible role in human cancers has been reported (reviewed in Huber et al., 2008).
RUVBL2 is overexpressed in hepatocellular carcinoma (Rousseau et al., 2007). Overexpression of RUVBL2 in several cancers and its possible role in human cancers has been reported (reviewed in Huber et al., 2008).
Localisation
Cytoplasm and nucleus.
Function
RUVBL2 interacts with c-myc (Wood et al., 2000) and also modulates transcriptional regulation by the beta-catenin/TCF-LEF complex (Bauer et al., 2000) and ATF2 (Cho et al., 2001).
RUVBL2 participates in the remodelling of chromatin as a member of several complexes such as TIP60 (Ikura et al., 2000), INO80 (Jin et al., 2005), SRCAP (Cai et al., 2005).
It is also involved in transcriptional regulation (reviewed in Gallant, 2007), DNA repair (Gospodinov et al., 2008), snoRNP biogenesis (Watkins et al., 2002), and telomerase activity (Venteicher et al., 2008).
RUVBL2 silencing in fibroblasts induces a senescent phenotype (Chan et al., 2005).
RUVBL2 participates in the remodelling of chromatin as a member of several complexes such as TIP60 (Ikura et al., 2000), INO80 (Jin et al., 2005), SRCAP (Cai et al., 2005).
It is also involved in transcriptional regulation (reviewed in Gallant, 2007), DNA repair (Gospodinov et al., 2008), snoRNP biogenesis (Watkins et al., 2002), and telomerase activity (Venteicher et al., 2008).
RUVBL2 silencing in fibroblasts induces a senescent phenotype (Chan et al., 2005).
Implicated in
Entity name
Hepatocellular carcinoma (HCC)
Disease
RUVBL2 was found to be overexpressed in 75% of cases in a series of 96 human HCC studied with real-time RT-PCR (Rousseau et al., 2007). It was also increased in a smaller 15 cases series (Iizuka et al., 2006). No mutations in the coding sequence were identified (Rousseau et al., 2007).
Prognosis
Overexpression of RUVBL2 was an independent factor of poor prognosis (Rousseau et al., 2007).
Oncogenesis
RUVBL2 depletion with siRNAs led to HCC cell growth arrest and apoptosis, whereas overexpression in HCC cells allowed these cells to give rise to more progressive tumors in xenografts than control cells (Rousseau et al., 2007).
Entity name
Colon cancer
Disease
RUVBL2 was overexpressed in a series of 18 colon cancers (Graudens et al., 2006).
Entity name
Melanoma
Disease
RUVBL2 was overexpressed in a series of 45 melanomas (Talantov et al., 2005).
Entity name
Bladder carcinoma
Disease
RUVBL2 was overexpressed in a series of 108 bladder carcinomas (Sanchez-Carbayo et al., 2006).
Entity name
Prostate cancer
Oncogenesis
In conjunction with beta-catenin, RUVBL2 represses the expression of the anti-metastasis gene KAI-1 (Kim et al., 2005) and is involved in the invasive phenotype of cultured prostate cancer cells (Kim et al., 2006).
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 11080158 | 2000 | Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity. | Bauer A et al |
| 15647280 | 2005 | The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes. | Cai Y et al |
| 15655109 | 2005 | The p400 E1A-associated protein is a novel component of the p53 --> p21 senescence pathway. | Chan HM et al |
| 11713276 | 2001 | TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage. | Cho SG et al |
| 17320397 | 2007 | Control of transcription by Pontin and Reptin. | Gallant P et al |
| 18834951 | 2009 | RAD51 foci formation in response to DNA damage is modulated by TIP49. | Gospodinov A et al |
| 16542501 | 2006 | Deciphering cellular states of innate tumor drug responses. | Graudens E et al |
| 18757398 | 2008 | Pontin and reptin, two related ATPases with multiple roles in cancer. | Huber O et al |
| 16703398 | 2006 | Involvement of c-myc-regulated genes in hepatocellular carcinoma related to genotype-C hepatitis B virus. | Iizuka N et al |
| 10966108 | 2000 | Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis. | Ikura T et al |
| 16230350 | 2005 | A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex. | Jin J et al |
| 16699503 | 2006 | Roles of sumoylation of a reptin chromatin-remodelling complex in cancer metastasis. | Kim JH et al |
| 15829968 | 2005 | Transcriptional regulation of a metastasis suppressor gene by Tip60 and beta-catenin complexes. | Kim JH et al |
| 17525332 | 2007 | ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. | Matsuoka S et al |
| 10998447 | 2000 | Human TIP49b/RUVBL2 gene: genomic structure, expression pattern, physical link to the human CGB/LHB gene cluster on chromosome 19q13.3. | Parfait B et al |
| 17157868 | 2007 | Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. | Puri T et al |
| 17657734 | 2007 | Overexpression and role of the ATPase and putative DNA helicase RuvB-like 2 in human hepatocellular carcinoma. | Rousseau B et al |
| 10524211 | 1999 | Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins. | Salzer U et al |
| 16432078 | 2006 | Defining molecular profiles of poor outcome in patients with invasive bladder cancer using oligonucleotide microarrays. | Sanchez-Carbayo M et al |
| 16243793 | 2005 | Novel genes associated with malignant melanoma but not benign melanocytic lesions. | Talantov D et al |
| 18358808 | 2008 | Identification of ATPases pontin and reptin as telomerase components essential for holoenzyme assembly. | Venteicher AS et al |
| 12417735 | 2002 | Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP. | Watkins NJ et al |
| 16014379 | 2005 | The histidine triad protein Hint1 interacts with Pontin and Reptin and inhibits TCF-beta-catenin-mediated transcription. | Weiske J et al |
| 10882073 | 2000 | An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. | Wood MA et al |
Other Information
Locus ID:
NCBI: 10856
MIM: 604788
HGNC: 10475
Ensembl: ENSG00000183207
Variants:
dbSNP: 10856
ClinVar: 10856
TCGA: ENSG00000183207
COSMIC: RUVBL2
RNA/Proteins
Expression (GTEx)
Pathways
Protein levels (Protein atlas)
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 37962355 | 2023 | Adenovirus E1A binding to DCAF10 targets proteasomal degradation of RUVBL1/2 AAA+ ATPases required for quaternary assembly of multiprotein machines, innate immunity, and responses to metabolic stress. | 0 |
| 37962355 | 2023 | Adenovirus E1A binding to DCAF10 targets proteasomal degradation of RUVBL1/2 AAA+ ATPases required for quaternary assembly of multiprotein machines, innate immunity, and responses to metabolic stress. | 0 |
| 33367824 | 2021 | NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis. | 11 |
| 33865222 | 2021 | Novel HIF-2α interaction with Reptin52 impairs HIF-2 transcriptional activity and EPO secretion. | 4 |
| 33894417 | 2021 | HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma. | 3 |
| 34072766 | 2021 | RuvB-Like Protein 2 Interacts with the NS1 Protein of Influenza A Virus and Affects Apoptosis That Is Counterbalanced by Type I Interferons. | 1 |
| 33367824 | 2021 | NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis. | 11 |
| 33865222 | 2021 | Novel HIF-2α interaction with Reptin52 impairs HIF-2 transcriptional activity and EPO secretion. | 4 |
| 33894417 | 2021 | HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma. | 3 |
| 34072766 | 2021 | RuvB-Like Protein 2 Interacts with the NS1 Protein of Influenza A Virus and Affects Apoptosis That Is Counterbalanced by Type I Interferons. | 1 |
| 33205750 | 2020 | Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. | 7 |
| 33355144 | 2020 | Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. | 24 |
| 33205750 | 2020 | Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. | 7 |
| 33355144 | 2020 | Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. | 24 |
| 31018511 | 2019 | Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein. | 10 |
Citation
Aude Grigoletto ; Valérie Haurie ; Jean Rosenbaum
RUVBL2 (RuvB-like 2 (E. coli))
Atlas Genet Cytogenet Oncol Haematol. 2009-03-01
Online version: http://atlasgeneticsoncology.org/gene/42185/ruvbl2
