EIF3F (eukaryotic translation initiation factor 3, subunit F)

2009-07-01 Jiaqi Shi , Mark A Nelson Affiliation

Department of Surgery, Arizona Cancer Center, University of Arizona, USA (JS); Department of Pathology, Arizona Cancer Center, University of Arizona, USA (JS, MN)

Identity

HGNC

EIF3F

LOCATION

11p15.4

LOCUSID

8665

ALIAS

EIF3S5,MRT67,eIF3-p47

DNA/RNA

Description

10.82kb long.

Transcription

8 exons; transcript length: 3,228 bps; 1073 bp coding sequence.

Pseudogene

Chromosome 2 from 58,332,079 to 58,333,164 bp (AC007250.3, Ensembl).

Proteins

Description

357 amino acids; 37,563.75 Da; Isoelectric point: 5.122; Mov34/MPN/PAD1 domain.

Expression

Ubiquitous.

Localisation

Cytoplasmic. There is also evidence of its existence in the nucleus.

Function

eIF3f is a subunit of eIF3 complex and is highly conserved among species. eIF3f interacts with the caspase-processed isoform of CDK11 (CDK11^p46), which appears to be a down-stream effector in apoptotic signaling. eIF3f can be phosphorylated at Ser46 and Thr119 by CDK11^p46 during apoptosis. The phosphorylation of eIF3f contributes to translation inhibition and apoptosis. The expression of eIF3f is significantly decreased in pancreatic cancer and melanoma. Loss of the eIF3f allele has been reported in pancreatic cancer and melanoma. Ectopic expression of eIF3f causes rRNA degradation, inhibits translation and cell proliferation, and induces apoptosis in pancreatic cancer and melanoma cells. On the other hand, knockdown of eIF3f prevents apoptosis in pancreatic cancer and melanoma cells. Recent studies also showed decreased cell growth, cell proliferation, colony formation and increased apoptosis in eIF3f-overexpressing NIH3T3 cells.

Homology

eIF3f is not found in S. cerevisiae; However it is found in Schizosaccharomyces pombe (SPBC4C3.07, 35% identity) and in Drosophila Melanogaster (CG9769, 49% identity).

Mutations

Note

No eIF3f mutations have been reported.

Implicated in

Entity name

Pancreatic cancer

Disease

Decreased eIF3f mRNA and protein was found in pancreatic cancer.

Oncogenesis

Decreased eIF3f gene expression may result from loss of eIF3f gene allele and down regulation of transcription. Overexpression of eIF3f in pancreatic cancer and melanoma cells can lead to 28S rRNA degradation, decreased translation and increased apoptosis. On the other hand, knockdown of eIF3f attenuates apoptosis in tumor cells.

Entity name

Melanoma

Disease

Decreased eIF3f mRNA and protein was found in melanoma.

Oncogenesis

See above

Entity name

Muscle atrophy

Disease

eIF3f is a key target of MAFbx, an E3 ubiquitin ligase, during muscle atrophy and has a major role in skeletal muscle hypertrophy. During muscle atrophy, MAFbx targets eIF3f for ubiquitination and degradation. Thus, eIF3f appears to be an attractive therapeutic target.

Article Bibliography

Pubmed ID	Last Year	Title	Authors
18381585	2008	Loss of the eukaryotic initiation factor 3f in melanoma.	Doldan A et al
18658124	2008	All translation elongation factors and the e, f, and h subunits of translation initiation factor 3 are encoded by 5'-terminal oligopyrimidine (TOP) mRNAs.	Iadevaia V et al
19289598	2009	mRNA retrotransposition coupled with 5' inversion as a possible source of new genes.	Kojima KK et al
18354498	2008	The initiation factor eIF3-f is a major target for atrogin1/MAFbx function in skeletal muscle atrophy.	Lagirand-Cantaloube J et al
17581632	2007	Reconstitution reveals the functional core of mammalian eIF3.	Masutani M et al
12446680	2003	The p34cdc2-related cyclin-dependent kinase 11 interacts with the p47 subunit of eukaryotic initiation factor 3 during apoptosis.	Shi J et al
19245811	2009	Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis.	Shi J et al
16532022	2006	Decreased expression of eukaryotic initiation factor 3f deregulates translation and apoptosis in tumor cells.	Shi J et al
19237569	2009	Inhibition of HIV-1 replication by eIF3f.	Valente ST et al

Other Information

Locus ID:

NCBI: 8665
MIM: 603914
HGNC: 3275
Ensembl: ENSG00000175390

Variants:

dbSNP: 8665
ClinVar: 8665
TCGA: ENSG00000175390
COSMIC: EIF3F

RNA/Proteins

Gene ID	Transcript ID	Uniprot
ENSG00000175390	ENST00000531329	E9PQV8
ENSG00000175390	ENST00000531572	H0YDT6
ENSG00000175390	ENST00000533626	O00303
ENSG00000175390	ENST00000640290	A0A1W2PP79
ENSG00000175390	ENST00000651655	O00303

Expression (GTEx)

100

150

Adipose - Subcutaneous

Adipose - Visceral

Adrenal Gland

Artery - Aorta

Artery - Tibial

Bladder

Brain - Amygdala

Brain - Anterior cingulate cortex

Brain - Caudate

Brain - Cerebellar Hemisphere

Brain - Cerebellum

Brain - Cortex

Brain - Frontal Cortex

Brain - Hippocampus

Brain - Hypothalamus

Brain - Nucleus accumbens

Brain - Putamen

Brain - Spinal cord

Brain - Substantia nigra

Breast - Mammary Tissue

Cells - Cultured fibroblasts

Cells - EBV - transformed lymphocytes

Cervix - Ectocervix

Cervix - Endocervix

Colon - Sigmoid

Colon - Transverse

Esophagus - Gastroesophageal Junction

Esophagus - Mucosa

Esophagus - Muscularis

Fallopian Tube

Heart - Atrial Appendage

Heart - Left Ventricle

Kidney - Cortex

Kidney - Medulla

Liver

Lung

Minor Salivary Gland

Muscle - Skeletal

Nerve - Tibial

Ovary

Pancreas

Pituitary

Prostate

Skin - Not Sun Exposed

Skin - Sun Exposed

Small Intestine - Terminal Ileum

Spleen

Stomach

Testis

Thyroid

Uterus

Vagina

Whole Blood

Pathways

Pathway	Source	External ID
RNA transport	KEGG	ko03013
RNA transport	KEGG	hsa03013
Metabolism of proteins	REACTOME	R-HSA-392499
Translation	REACTOME	R-HSA-72766
Eukaryotic Translation Initiation	REACTOME	R-HSA-72613
Cap-dependent Translation Initiation	REACTOME	R-HSA-72737
Formation of a pool of free 40S subunits	REACTOME	R-HSA-72689
Formation of the ternary complex, and subsequently, the 43S complex	REACTOME	R-HSA-72695
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S	REACTOME	R-HSA-72662
Translation initiation complex formation	REACTOME	R-HSA-72649
Ribosomal scanning and start codon recognition	REACTOME	R-HSA-72702
GTP hydrolysis and joining of the 60S ribosomal subunit	REACTOME	R-HSA-72706
L13a-mediated translational silencing of Ceruloplasmin expression	REACTOME	R-HSA-156827
Gene Expression	REACTOME	R-HSA-74160

Protein levels (Protein atlas)

Not detected

Low

Medium

High

References

Pubmed ID	Year	Title	Citations
37544925	2023	eIF3f Mediates SGOC Pathway Reprogramming by Enhancing Deubiquitinating Activity in Colorectal Cancer.	3
37544925	2023	eIF3f Mediates SGOC Pathway Reprogramming by Enhancing Deubiquitinating Activity in Colorectal Cancer.	3
33736665	2021	EIF3F-related neurodevelopmental disorder: refining the phenotypic and expanding the molecular spectrum.	3
33736665	2021	EIF3F-related neurodevelopmental disorder: refining the phenotypic and expanding the molecular spectrum.	3
31527668	2020	Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F.	11
31527668	2020	Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F.	11
30573685	2019	Estrogen receptor α promotes protein synthesis by fine-tuning the expression of the eukaryotic translation initiation factor 3 subunit f (eIF3f).	14
30573685	2019	Estrogen receptor α promotes protein synthesis by fine-tuning the expression of the eukaryotic translation initiation factor 3 subunit f (eIF3f).	14
26988917	2016	eIF3f reduces tumor growth by directly interrupting clusterin with anti-apoptotic property in cancer cells.	10
26988917	2016	eIF3f reduces tumor growth by directly interrupting clusterin with anti-apoptotic property in cancer cells.	10
26497985	2015	The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity.	2
26497985	2015	The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity.	2
24678890	2014	Decreased expression of eukaryotic initiation factor 3f is an adverse prognostic factor for stage I-III gastric cancer.	5
24732644	2014	Expression of eukaryotic initiation factor 3f is associated with prognosis in gastric carcinomas.	7
24678890	2014	Decreased expression of eukaryotic initiation factor 3f is an adverse prognostic factor for stage I-III gastric cancer.	5

Citation

Jiaqi Shi ; Mark A Nelson

EIF3F (eukaryotic translation initiation factor 3, subunit F)

Atlas Genet Cytogenet Oncol Haematol. 2009-07-01

Online version: http://atlasgeneticsoncology.org/gene/44407/meetings/favicon/css/card-gene.css