BLCAP (bladder cancer associated protein)

2008-05-01   Wu Xinxing , Zhao Min , Zuo Zehua 

School of Medicine, Wuhan University, Wuhan, Hubei, 430071, P.R. China

Identity

HGNC
LOCATION
20q11.23
LOCUSID
ALIAS
BC10
FUSION GENES

DNA/RNA

Description

The BLCAP gene located in chromosome 20q11.2-q12, consists of only one exon and the coding region spans 264 bp. The comparability between the sequences of BLCAP gene and Homo sapiens mRNA (DKFZP564M053) or BC10 was 99% and 87% respectively.

Proteins

Expression

Expression in human tissues with a relatively low level of expression in cervix, bladder, testis, placenta, spleen and peripheral blood leukocytes.

Function

BLCAP protein was a member of transmembrane protein 1. By analyzing the signal peptide of BLCAP protein, we found a cleavage site in 63-64 aa. It showed that there might be three phospholate sites: 68 aa, 73 aa and 78 aa. In 78-81 aa, a typical [ST]-X(2)-[DE] structure- the phospholate site of tyrosine protein kinase was found.

Homology

The comparability between the sequences of BLCAP gene and Homo sapiens mRNA (DKFZP564M053) or BC10 was 99% and 87% respectively.

Implicated in

Entity name
Cervical cancer
Disease
The bladder cancer-associated protein gene (BLCAP) was among the differentially expressed genes in cervical cancer. BLCAP is expressed highly in non cancerous cervical tissues, but it is greatly lost in primary cervical cancer tissue. After transfected BLCAP cDNA into HeLa cells, HeLa cells shows reduced cell growth and clone genicity compared to the vector-transfected cognate cells. BLCAP expression in HeLa cells leads to growth arrest and significantly enhanced apoptosis in vitro and reduced tumor formation in vivo.
Entity name
Bladder cancer
Disease
The bladder cancer-associated protein gene (BLCAP, bladder cancer, Mr 10 kDa) was exclusively expressed in the non invasive lesions of bladder cancer. The full-length bc10 cDNA contains a complete open reading frame (ORF) of 263 bp and encodes a protein composed of 87 amino acids that has no homology to any of the known protein families. Transient expression of bc10 cDNA in COS1 cells yielded a primary translation product with an apparent Mr of 9.8 kDa and pI of 6.7, in agreement with the theoretical calculated value. Comparison of mouse and human bc10 genomic loci revealed an intron less organization of the coding region in both species as well as a highly conserved structure having 91% and 100% identity at the DNA (coding region) and protein levels, respectively. Southern analysis did not reveal gross DNA rearrangements within the bc10 genomic locus in the invasive bladder tumors, implying that the differential expression of the gene most likely reflects alterations in messenger expression (transcription and/or mRNA decay). The downregulation of this novel marker in invasive tumors suggests a putative role in bladder cancer progression.
Entity name
Tongue carcinoma
Disease
Bladder cancer-associated protein gene (BLCAP) is a novel candidate tumor suppressor gene identified from the human bladder carcinoma. BLCAP overexpression could inhibit cell growth by inducing apoptosis in HeLa cells Such evidence suggests the alterations in BLCAP may play an important role in tumorigenesis. Overexpressed BLCAP resulted in growth inhibition of the human tongue cancer cell line Tca8113 in vitro, accompanied by S phase cell cycle arrest and apoptosis. The growth inhibition was correlated with up-regulation of p21 (WAF1/CIP1 ) expression and down-regulation of Bcl-XL and Bcl-2 expressions. However, p53 expression and NF-kappaB activity remained unchanged post infection. Furthermore, no changes in p53 phosphorylation at Ser46 and nuclear localization, which are critical to p53 function, were observed in BLCAP-overexpressed cells. Taken together, BLCAP may play a role not only in regulating cell proliferation but also in coordinating apoptosis and cell cycle via a novel way independent of p53 and NF-kappaB.
Entity name
Disease
The positive rate of BLCAP protein in primary osteosarcoma was very high, much higher than that in recurrent osteosarcoma. It may be helpful for evaluating the prognosis of osteosarcoma to study the relationship between expression of BLCAP protein and malignancy of osteosarcoma.

Bibliography

Pubmed IDLast YearTitleAuthors
157979042005A bioinformatic screen for novel A-I RNA editing sites reveals recoding editing in BC10.Clutterbuck DR et al
159018422005Comparative phylogenetic analysis of blcap/nnat reveals eutherian-specific imprinted gene.Evans HK et al
119206132002bc10: A novel human bladder cancer-associated protein with a conserved genomic structure downregulated in invasive cancer.Gromova I et al
184073642008Determination of editors at the novel A-to-I editing positions.Nishimoto Y et al
151696582003[Relationship between expression of BLCAP protein and malignancy of osteosarcoma].Su HC et al
170315752007Overexpression of BLCAP induces S phase arrest and apoptosis independent of p53 and NF-kappaB in human tongue carcinoma : BLCAP overexpression induces S phase arrest and apoptosis.Yao J et al
166759152006Functional analysis of bladder cancer-related protein gene: a putative cervical cancer tumor suppressor gene in cervical carcinoma.Zuo Z et al
168312692006[Inhibitory effect of bladder cancer related protein gene on HeLa cell proliferation].Zuo ZH et al

Other Information

Locus ID:

NCBI: 10904
MIM: 613110
HGNC: 1055
Ensembl: ENSG00000166619

Variants:

dbSNP: 10904
ClinVar: 10904
TCGA: ENSG00000166619
COSMIC: BLCAP

RNA/Proteins

Gene IDTranscript IDUniprot
ENSG00000166619ENST00000373537P62952
ENSG00000166619ENST00000397131P62952
ENSG00000166619ENST00000397134P62952
ENSG00000166619ENST00000397135P62952
ENSG00000166619ENST00000397137P62952
ENSG00000166619ENST00000414080A2A2K9
ENSG00000166619ENST00000414542P62952
ENSG00000166619ENST00000432507P62952
ENSG00000166619ENST00000445723A2A2K8
ENSG00000166619ENST00000456058A0A1Y8ELN7

Expression (GTEx)

0
10
20
30
40
50
60
70
80
90
100

References

Pubmed IDYearTitleCitations
199082602010Human BLCAP transcript: new editing events in normal and cancerous tissues.26
188362092009Transcript- and tissue-specific imprinting of a tumour suppressor gene.20
197837932010Bladder cancer-associated protein, a potential prognostic biomarker in human bladder cancer.17
170315752007Overexpression of BLCAP induces S phase arrest and apoptosis independent of p53 and NF-kappaB in human tongue carcinoma : BLCAP overexpression induces S phase arrest and apoptosis.13
254990812015RNA over-editing of BLCAP contributes to hepatocarcinogenesis identified by whole-genome and transcriptome sequencing.12
279385052017Upregulated miR-9-3p Promotes Cell Growth and Inhibits Apoptosis in Medullary Thyroid Carcinoma by Targeting BLCAP.9
284559602017A-to-I RNA editing of BLCAP lost the inhibition to STAT3 activation in cervical cancer.8
166759152006Functional analysis of bladder cancer-related protein gene: a putative cervical cancer tumor suppressor gene in cervical carcinoma.7
230499072012Immunoexpression analysis and prognostic value of BLCAP in breast cancer.5
306838072019Long non-coding RNA MALAT1 regulates BLCAP mRNA expression through binding to miR-339-5p and promotes poor prognosis in breast cancer.4

Citation

Wu Xinxing ; Zhao Min ; Zuo Zehua

BLCAP (bladder cancer associated protein)

Atlas Genet Cytogenet Oncol Haematol. 2008-05-01

Online version: http://atlasgeneticsoncology.org/gene/801/blcap