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BLCAP (bladder cancer associated protein)

Identity

Other namesBC10
HGNC (Hugo) BLCAP
LocusID (NCBI) 10904
Location 20q11.23
Location_base_pair Starts at 36145819 and ends at 36156333 bp from pter ( according to hg19-Feb_2009)  [Mapping]
Note Bladder cancer associated protein is a protein which is believed to be involved in the cancercinogenesis. Lower levels of BLCAP have been identified in advanced bladder cancer and cervical carcinoma, suggesting a correlation between lower-expression of BLCAP and tumor development.

DNA/RNA

Description The BLCAP gene located in chromosome 20q11.2-q12, consists of only one exon and the coding region spans 264 bp. The comparability between the sequences of BLCAP gene and Homo sapiens mRNA (DKFZP564M053) or BC10 was 99% and 87% respectively.

Protein

Description The protein encoded by BLCAP were composite of Leu(19.5%), Pro(9.19%), ser(8.04%), cys(8.04%) and other amino acids. The N-terminal of the protein which was encoded by BLCAP gene was an alpha helix. At the C-terminal, it was beta sheet and in the middle, it was coil. The two terminal regions were more hydrophobile than the middleregion. Between 45-65 aa, there was a transmembrane region.
Expression Expression in human tissues with a relatively low level of expression in cervix, bladder, testis, placenta, spleen and peripheral blood leukocytes.
Function BLCAP protein was a member of transmembrane protein 1. By analyzing the signal peptide of BLCAP protein, we found a cleavage site in 63-64 aa. It showed that there might be three phospholate sites: 68 aa, 73 aa and 78 aa. In 78-81 aa, a typical [ST]-X(2)-[DE] structure- the phospholate site of tyrosine protein kinase was found.
Homology The comparability between the sequences of BLCAP gene and Homo sapiens mRNA (DKFZP564M053) or BC10 was 99% and 87% respectively.

Implicated in

Entity Cervical cancer
Disease The bladder cancer-associated protein gene (BLCAP) was among the differentially expressed genes in cervical cancer. BLCAP is expressed highly in non cancerous cervical tissues, but it is greatly lost in primary cervical cancer tissue. After transfected BLCAP cDNA into HeLa cells, HeLa cells shows reduced cell growth and clone genicity compared to the vector-transfected cognate cells. BLCAP expression in HeLa cells leads to growth arrest and significantly enhanced apoptosis in vitro and reduced tumor formation in vivo.
  
Entity Bladder cancer
Disease The bladder cancer-associated protein gene (BLCAP, bladder cancer, Mr 10 kDa) was exclusively expressed in the non invasive lesions of bladder cancer. The full-length bc10 cDNA contains a complete open reading frame (ORF) of 263 bp and encodes a protein composed of 87 amino acids that has no homology to any of the known protein families. Transient expression of bc10 cDNA in COS1 cells yielded a primary translation product with an apparent Mr of 9.8 kDa and pI of 6.7, in agreement with the theoretical calculated value. Comparison of mouse and human bc10 genomic loci revealed an intron less organization of the coding region in both species as well as a highly conserved structure having 91% and 100% identity at the DNA (coding region) and protein levels, respectively. Southern analysis did not reveal gross DNA rearrangements within the bc10 genomic locus in the invasive bladder tumors, implying that the differential expression of the gene most likely reflects alterations in messenger expression (transcription and/or mRNA decay). The downregulation of this novel marker in invasive tumors suggests a putative role in bladder cancer progression.
  
Entity Tongue carcinoma
Disease Bladder cancer-associated protein gene (BLCAP) is a novel candidate tumor suppressor gene identified from the human bladder carcinoma. BLCAP overexpression could inhibit cell growth by inducing apoptosis in HeLa cells Such evidence suggests the alterations in BLCAP may play an important role in tumorigenesis. Overexpressed BLCAP resulted in growth inhibition of the human tongue cancer cell line Tca8113 in vitro, accompanied by S phase cell cycle arrest and apoptosis. The growth inhibition was correlated with up-regulation of p21 (WAF1/CIP1 ) expression and down-regulation of Bcl-XL and Bcl-2 expressions. However, p53 expression and NF-kappaB activity remained unchanged post infection. Furthermore, no changes in p53 phosphorylation at Ser46 and nuclear localization, which are critical to p53 function, were observed in BLCAP-overexpressed cells. Taken together, BLCAP may play a role not only in regulating cell proliferation but also in coordinating apoptosis and cell cycle via a novel way independent of p53 and NF-kappaB.
  
Entity Osteosarcoma
Disease The positive rate of BLCAP protein in primary osteosarcoma was very high, much higher than that in recurrent osteosarcoma. It may be helpful for evaluating the prognosis of osteosarcoma to study the relationship between expression of BLCAP protein and malignancy of osteosarcoma.
  

External links

Nomenclature
HGNC (Hugo)BLCAP   1055
Cards
AtlasBLCAPID801ch20q11
Entrez_Gene (NCBI)BLCAP  10904  bladder cancer associated protein
GeneCards (Weizmann)BLCAP
Ensembl (Hinxton)ENSG00000166619 [Gene_View]  chr20:36145819-36156333 [Contig_View]  BLCAP [Vega]
ICGC DataPortalENSG00000166619
cBioPortalBLCAP
AceView (NCBI)BLCAP
Genatlas (Paris)BLCAP
WikiGenes10904
SOURCE (Princeton)NM_001167820 NM_001167821 NM_001167822 NM_001167823 NM_006698
Genomic and cartography
GoldenPath (UCSC)BLCAP  -  20q11.23   chr20:36145819-36156333 -  20q11.23   [Description]    (hg19-Feb_2009)
EnsemblBLCAP - 20q11.23 [CytoView]
Mapping of homologs : NCBIBLCAP [Mapview]
OMIM613110   
Gene and transcription
Genbank (Entrez)AA780553 AF053470 AI920860 AK074498 AK124822
RefSeq transcript (Entrez)NM_001167820 NM_001167821 NM_001167822 NM_001167823 NM_006698
RefSeq genomic (Entrez)AC_000152 NC_000020 NC_018931 NT_011362 NW_001838665 NW_004929418
Consensus coding sequences : CCDS (NCBI)BLCAP
Cluster EST : UnigeneHs.472651 [ NCBI ]
CGAP (NCI)Hs.472651
Alternative Splicing : Fast-db (Paris)GSHG0019170
Alternative Splicing GalleryENSG00000166619
Gene ExpressionBLCAP [ NCBI-GEO ]     BLCAP [ SEEK ]   BLCAP [ MEM ]
Protein : pattern, domain, 3D structure
UniProt/SwissProtP62952 (Uniprot)
NextProtP62952  [Medical]
With graphics : InterProP62952
Splice isoforms : SwissVarP62952 (Swissvar)
Domains : Interpro (EBI)BC10 [organisation]   BC10-like [organisation]  
Related proteins : CluSTrP62952
Domain families : Pfam (Sanger)BC10 (PF06726)   
Domain families : Pfam (NCBI)pfam06726   
Domain structure : Prodom (Prabi Lyon)BC10-like (PD062192)   
DMDM Disease mutations10904
Blocks (Seattle)P62952
Human Protein AtlasENSG00000166619 [gene] [tissue] [antibody] [cell] [cancer]
Peptide AtlasP62952
HPRD16552
IPIIPI00030916   IPI00794784   IPI00794431   IPI00793823   
Protein Interaction databases
DIP (DOE-UCLA)P62952
IntAct (EBI)P62952
FunCoupENSG00000166619
BioGRIDBLCAP
InParanoidP62952
Interologous Interaction database P62952
IntegromeDBBLCAP
STRING (EMBL)BLCAP
Ontologies - Pathways
Ontology : AmiGOprotein binding  cell cycle  integral component of membrane  apoptotic nuclear changes  
Ontology : EGO-EBIprotein binding  cell cycle  integral component of membrane  apoptotic nuclear changes  
Protein Interaction DatabaseBLCAP
Wikipedia pathwaysBLCAP
Gene fusion - rearrangments
Polymorphisms : SNP, mutations, diseases
SNP Single Nucleotide Polymorphism (NCBI)BLCAP
snp3D : Map Gene to Disease10904
SNP (GeneSNP Utah)BLCAP
SNP : HGBaseBLCAP
Genetic variants : HAPMAPBLCAP
Exome VariantBLCAP
1000_GenomesBLCAP 
ICGC programENSG00000166619 
Somatic Mutations in Cancer : COSMICBLCAP 
CONAN: Copy Number AnalysisBLCAP 
Mutations and Diseases : HGMDBLCAP
Mutations and Diseases : intOGenBLCAP
Genomic VariantsBLCAP  BLCAP [DGVbeta]
dbVarBLCAP
ClinVarBLCAP
Pred. of missensesPolyPhen-2  SIFT(SG)  SIFT(JCVI)  Align-GVGD  MutAssessor  Mutanalyser  
Pred. splicesGeneSplicer  Human Splicing Finder  MaxEntScan  
Diseases
OMIM613110   
MedgenBLCAP
GENETestsBLCAP
Disease Genetic AssociationBLCAP
Huge Navigator BLCAP [HugePedia]  BLCAP [HugeCancerGEM]
General knowledge
Homologs : HomoloGeneBLCAP
Homology/Alignments : Family Browser (UCSC)BLCAP
Phylogenetic Trees/Animal Genes : TreeFamBLCAP
Chemical/Protein Interactions : CTD10904
Chemical/Pharm GKB GenePA25367
Clinical trialBLCAP
Cancer Resource (Charite)ENSG00000166619
Other databases
Probes
Litterature
PubMed16 Pubmed reference(s) in Entrez
CoreMineBLCAP
iHOPBLCAP
OncoSearchBLCAP

Bibliography

bc10: A novel human bladder cancer-associated protein with a conserved genomic structure downregulated in invasive cancer.
Gromova I, Gromov P, Celis JE.
Int J Cancer. 2002 Apr 1;98(4):539-46.
PMID 11920613
 
Relationship between expression of BLCAP protein and malignancy of osteosarcoma.
Su HC, Zhao YH, Fan DG, Fan QY, Zhang P, Wen YH, Liu YY.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Sep;19(5):465-6.
PMID 15169658
 
A bioinformatic screen for novel A-I RNA editing sites reveals recoding editing in BC10.
Clutterbuck DR, Leroy A, O'Connell MA, Semple CA.
Bioinformatics. 2005 Jun 1;21(11):2590-5.
PMID 15797904
 
Comparative phylogenetic analysis of blcap/nnat reveals eutherian-specific imprinted gene.
Evans HK, Weidman JR, Cowley DO, Jirtle RL.
Mol Biol Evol. 2005 Aug;22(8):1740-8.
PMID 15901842
 
Inhibitory effect of bladder cancer related protein gene on hela cell proliferation.
Zuo ZH, Zhao M, Liu J, Wei Y, Wu XX.
Ai Zheng. 2006 Jul;25(7):811-7.
PMID 16831269
 
Functional analysis of bladder cancer-related protein gene: a putative cervical cancer tumor suppressor gene in cervical carcinoma.
Zuo Z, Zhao M, Liu J, Gao G, Wu X.
Tumour Biol. 2006;27(4):221-6.
PMID 16675915
 
Overexpression of BLCAP induces S phase arrest and apoptosis independent of p53 and NF-kappaB in human tongue carcinoma : BLCAP overexpression induces S phase arrest and apoptosis.
Yao J, Duan L, Fan M, Yuan J, Wu X.
Mol Cell Biochem. 2007 Mar;297(1-2):81-92.
PMID 17031575
 
Determination of editors at the novel A-to-I editing positions.
Nishimoto Y, Yamashita T, Hideyama T, Tsuji S, Suzuki N, Kwak S.
Neurosci Res. 2008 Jun;61(2):201-6.
PMID 18407364
 
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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Contributor(s)

Written05-2008Wu Xinxing, Zhao Min, Zuo Zehua
School of Medicine, Wuhan University, Wuhan, Hubei, 430071, P.R. China

Citation

This paper should be referenced as such :
Xinxing, W ; Min, Z ; Zehua, Z
BLCAP (bladder cancer associated protein)
Atlas Genet Cytogenet Oncol Haematol. 2009;13(4):258-259.
Free online version   Free pdf version   [Bibliographic record ]
URL : http://AtlasGeneticsOncology.org/Genes/BLCAPID801ch20q11.html

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indexed on : Sat Oct 4 12:58:59 CEST 2014

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