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| | The N-terminal amino acids from 1-153 contains most of the functional domains consisting of the Kinetochore binding domain, Mad2 binding domain and the Anaphase promoting complex (APC) activation domain. Amino acids 129-499 contain the WD-repeat region. |
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| Description | Size: 499 amino acids; 54723 Da. Subunit: Interacts with MAD2L1. The phosphorylated form interacts with APC/C. Developmental stage: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. |
| Expression | It is expressed in all cells, no tissue specificity. |
| Localisation | Nucleus |
| Function | CDC20 is a key player in the Spindle Assembly Checkpoint (SAC). When a cell is dividing mitotically, the Metaphase to Anaphase transition is stringently monitored by SAC. After proper alignment of all the sister chromatids to the spindle fibers during metaphase, the Mitotic Checkpoint Complex detaches from CDC20 and free CDC20 protein activates the Anaphase promoting Complex (APC) Activated APC can then degrade Securin which frees the protease Separase. Free Separase can now degrade the Cohesin molecules binding the two sister chromatids together. Upon degradation of Cohesin, the two sister chromatids are free and can migrate to the two spindle poles, thus, initiating Anaphase. |
| Homology | The C-terminal half is highly conserved from humans to yeast. |
| Cdc20 associates with the kinase aurora2/Aik. |
| Farruggio DC, Townsley FM, Ruderman JV. |
| Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7306-11. |
| PMID 10377410 |
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| Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. |
| Fraschini R, Beretta A, Sironi L, Musacchio A, Lucchini G, Piatti S. |
| EMBO J. 2001 Dec 3;20(23):6648-59. |
| PMID 11726501 |
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| Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. |
| Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ. |
| J Cell Biol. 1998 Jun 15;141(6):1393-406. |
| PMID 9628895 |
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| CDC20, a potential cancer therapeutic target, is negatively regulated by p53. |
| Kidokoro T, Tanikawa C, Furukawa Y, Katagiri T, Nakamura Y, Matsuda K. |
| Oncogene. 2008 Mar 6;27(11):1562-71. |
| PMID 17873905 |
| |
| A new Mad2-interacting domain of Cdc20 is critical for the function of Mad2-Cdc20 complex in the spindle assembly checkpoint. |
| Mondal G, Baral RN, Roychoudhury S. |
| Biochem J. 2006 Jun 1;396(2):243-53. |
| PMID 16497171 |
| |
| Overexpression of Cdc20 leads to impairment of the spindle assembly checkpoint and aneuploidization in oral cancer. |
| Mondal G, Sengupta S, Panda CK, Gollin SM, Saunders WS, Roychoudhury S. |
| Carcinogenesis. 2007 Jan;28(1):81-92. |
| PMID 16777988 |
| |
| The spindle checkpoint: structural insights into dynamic signalling. |
| Musacchio A, Hardwick KG. |
| Nat Rev Mol Cell Biol. 2002 Oct;3(10):731-41. (Review) |
| PMID 12360190 |
| |
| Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint. |
| Sironi L, Melixetian M, Faretta M, Prosperini E, Helin K, Musacchio A. |
| EMBO J. 2001 Nov 15;20(22):6371-82. |
| PMID 11707408 |
| |
| Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. |
| Tang Z, Shu H, Oncel D, Chen S, Yu H. |
| Mol Cell. 2004 Nov 5;16(3):387-97. |
| PMID 15525512 |
| |
| Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1. |
| Weinstein J. |
| J Biol Chem. 1997 Nov 7;272(45):28501-11. |
| PMID 9353311 |
| |
| p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase. |
| Wu H, Lan Z, Li W, Wu S, Weinstein J, Sakamoto KM, Dai W. |
| Oncogene. 2000 Sep 21;19(40):4557-62 |
| PMID 11030144 |
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| Cdc20: a WD40 activator for a cell cycle degradation machine. |
| Yu H. |
| Mol Cell. 2007 Jul 6;27(1):3-16. |
| PMID 17612486 |
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