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CDC20 (cell division cycle 20 homolog (S. cerevisiae))

Written2008-04Susanta Roychoudhury, Taraswi Banerjee, Somsubhra Nath
Human Genetics, Genomics Division, Indian Institute of Chemical Biology, Kolkata-700 032, India

(Note : for Links provided by Atlas : click)

Identity

Other aliasCDC20A
MGC102824
P55CDC-LSB
bA276H19.3
p55CDC
LocusID (NCBI) 991
Atlas_Id 40003
Location 1p34.2  [Link to chromosome band 1p34]
Location_base_pair Starts at and ends at bp from pter
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)
CDC20 (1p34.2) / PIP4K2B (17q12)

DNA/RNA

Description Start: 43,597,199 bp from pter
End: 43,601,461 bp from pter
Size: 4,262 bases
Orientation: plus strand
13 exons (Entrez), 15 Exons (Ensembl)
Transcription 1697 bp
Pseudogene No

Protein

 
  The N-terminal amino acids from 1-153 contains most of the functional domains consisting of the Kinetochore binding domain, Mad2 binding domain and the Anaphase promoting complex (APC) activation domain. Amino acids 129-499 contain the WD-repeat region.
Description Size: 499 amino acids; 54723 Da. Subunit: Interacts with MAD2L1. The phosphorylated form interacts with APC/C. Developmental stage: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
Expression It is expressed in all cells, no tissue specificity.
Localisation Nucleus
Function CDC20 is a key player in the Spindle Assembly Checkpoint (SAC). When a cell is dividing mitotically, the Metaphase to Anaphase transition is stringently monitored by SAC. After proper alignment of all the sister chromatids to the spindle fibers during metaphase, the Mitotic Checkpoint Complex detaches from CDC20 and free CDC20 protein activates the Anaphase promoting Complex (APC) Activated APC can then degrade Securin which frees the protease Separase. Free Separase can now degrade the Cohesin molecules binding the two sister chromatids together. Upon degradation of Cohesin, the two sister chromatids are free and can migrate to the two spindle poles, thus, initiating Anaphase.
Homology The C-terminal half is highly conserved from humans to yeast.

Mutations

Germinal No
Somatic No

Implicated in

Note
  
Entity Various tumors
Disease There have been few reports of overexpression of CDC20 in various tumors for eg, greater than 3% of Cdc20 expression was found in bladder cancer, breast cancer, cervical cancer, cholangiocellular carcinoma, AML, CML, colon and rectum carcinoma, esophageal cancer, gastric cancer, gastric cancer (diffuse type), liver cancer, lung cancer (NSCLC), lung cancer (SCLC), osteosarcoma, pancreatic cancer, prostate cancer, renal carcinoma, soft tissue tumor, testicular tumor (Kidoko T, et al., 2007), head and neck cancer (Mondal G, et al., 2007).
Prognosis No
Cytogenetics No
Hybrid/Mutated Gene No
Abnormal Protein No
Oncogenesis Overexpression of CDC20 has been observed in several tumor tissues.
  

Bibliography

Cdc20 associates with the kinase aurora2/Aik.
Farruggio DC, Townsley FM, Ruderman JV.
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7306-11.
PMID 10377410
 
Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores.
Fraschini R, Beretta A, Sironi L, Musacchio A, Lucchini G, Piatti S.
EMBO J. 2001 Dec 3;20(23):6648-59.
PMID 11726501
 
Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events.
Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ.
J Cell Biol. 1998 Jun 15;141(6):1393-406.
PMID 9628895
 
CDC20, a potential cancer therapeutic target, is negatively regulated by p53.
Kidokoro T, Tanikawa C, Furukawa Y, Katagiri T, Nakamura Y, Matsuda K.
Oncogene. 2008 Mar 6;27(11):1562-71.
PMID 17873905
 
A new Mad2-interacting domain of Cdc20 is critical for the function of Mad2-Cdc20 complex in the spindle assembly checkpoint.
Mondal G, Baral RN, Roychoudhury S.
Biochem J. 2006 Jun 1;396(2):243-53.
PMID 16497171
 
Overexpression of Cdc20 leads to impairment of the spindle assembly checkpoint and aneuploidization in oral cancer.
Mondal G, Sengupta S, Panda CK, Gollin SM, Saunders WS, Roychoudhury S.
Carcinogenesis. 2007 Jan;28(1):81-92.
PMID 16777988
 
The spindle checkpoint: structural insights into dynamic signalling.
Musacchio A, Hardwick KG.
Nat Rev Mol Cell Biol. 2002 Oct;3(10):731-41. (Review)
PMID 12360190
 
Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.
Sironi L, Melixetian M, Faretta M, Prosperini E, Helin K, Musacchio A.
EMBO J. 2001 Nov 15;20(22):6371-82.
PMID 11707408
 
Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint.
Tang Z, Shu H, Oncel D, Chen S, Yu H.
Mol Cell. 2004 Nov 5;16(3):387-97.
PMID 15525512
 
Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1.
Weinstein J.
J Biol Chem. 1997 Nov 7;272(45):28501-11.
PMID 9353311
 
p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase.
Wu H, Lan Z, Li W, Wu S, Weinstein J, Sakamoto KM, Dai W.
Oncogene. 2000 Sep 21;19(40):4557-62
PMID 11030144
 
Cdc20: a WD40 activator for a cell cycle degradation machine.
Yu H.
Mol Cell. 2007 Jul 6;27(1):3-16.
PMID 17612486
 

Citation

This paper should be referenced as such :
Roychoudhury, S ; Banerjee, T ; Nath, S. CDC20 (cell division cycle 20 homolog (S
cerevisiae))
Atlas Genet Cytogenet Oncol Haematol. 2009;13(2):104-105.
Free journal version : [ pdf ]   [ DOI ]
On line version : http://AtlasGeneticsOncology.org/Genes/CDC20ID40003ch1p34.html


External links

Nomenclature
Cards
AtlasCDC20ID40003ch1p34.txt
Aliases
Genomic and cartography
Gene and transcription
RefSeq transcript (Entrez)
RefSeq genomic (Entrez)
SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
BioGPS (Tissue expression)991
Protein : pattern, domain, 3D structure
Domain families : Pfam (Sanger)
Domain families : Pfam (NCBI)
Protein Interaction databases
Ontologies - Pathways
Clinical trials, drugs, therapy
Miscellaneous
canSAR (ICR) (select the gene name)
Probes
Litterature
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed


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