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CDC20 (cell division cycle 20 homolog (S. cerevisiae))

Written2008-04Susanta Roychoudhury, Taraswi Banerjee, Somsubhra Nath
Human Genetics, Genomics Division, Indian Institute of Chemical Biology, Kolkata-700 032, India

(Note : for Links provided by Atlas : click)

Identity

Alias_namesCDC20 (cell division cycle 20, S. cerevisiae, homolog)
CDC20 cell division cycle 20 homolog (S. cerevisiae)
cell division cycle 20 homolog (S. cerevisiae)
Alias_symbol (synonym)p55CDC
CDC20A
Other aliasMGC102824
P55CDC-LSB
bA276H19.3
HGNC (Hugo) CDC20
LocusID (NCBI) 991
Atlas_Id 40003
Location 1p34.2  [Link to chromosome band 1p34]
Location_base_pair Starts at 43358955 and ends at 43363202 bp from pter ( according to hg19-Feb_2009)  [Mapping CDC20.png]
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)
CDC20 (1p34.2) / PIP4K2B (17q12)

DNA/RNA

Description Start: 43,597,199 bp from pter
End: 43,601,461 bp from pter
Size: 4,262 bases
Orientation: plus strand
13 exons (Entrez), 15 Exons (Ensembl)
Transcription 1697 bp
Pseudogene No

Protein

 
  The N-terminal amino acids from 1-153 contains most of the functional domains consisting of the Kinetochore binding domain, Mad2 binding domain and the Anaphase promoting complex (APC) activation domain. Amino acids 129-499 contain the WD-repeat region.
Description Size: 499 amino acids; 54723 Da. Subunit: Interacts with MAD2L1. The phosphorylated form interacts with APC/C. Developmental stage: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
Expression It is expressed in all cells, no tissue specificity.
Localisation Nucleus
Function CDC20 is a key player in the Spindle Assembly Checkpoint (SAC). When a cell is dividing mitotically, the Metaphase to Anaphase transition is stringently monitored by SAC. After proper alignment of all the sister chromatids to the spindle fibers during metaphase, the Mitotic Checkpoint Complex detaches from CDC20 and free CDC20 protein activates the Anaphase promoting Complex (APC) Activated APC can then degrade Securin which frees the protease Separase. Free Separase can now degrade the Cohesin molecules binding the two sister chromatids together. Upon degradation of Cohesin, the two sister chromatids are free and can migrate to the two spindle poles, thus, initiating Anaphase.
Homology The C-terminal half is highly conserved from humans to yeast.

Mutations

Germinal No
Somatic No

Implicated in

Note
  
Entity Various tumors
Disease There have been few reports of overexpression of CDC20 in various tumors for eg, greater than 3% of Cdc20 expression was found in bladder cancer, breast cancer, cervical cancer, cholangiocellular carcinoma, AML, CML, colon and rectum carcinoma, esophageal cancer, gastric cancer, gastric cancer (diffuse type), liver cancer, lung cancer (NSCLC), lung cancer (SCLC), osteosarcoma, pancreatic cancer, prostate cancer, renal carcinoma, soft tissue tumor, testicular tumor (Kidoko T, et al., 2007), head and neck cancer (Mondal G, et al., 2007).
Prognosis No
Cytogenetics No
Hybrid/Mutated Gene No
Abnormal Protein No
Oncogenesis Overexpression of CDC20 has been observed in several tumor tissues.
  

Bibliography

Cdc20 associates with the kinase aurora2/Aik.
Farruggio DC, Townsley FM, Ruderman JV.
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7306-11.
PMID 10377410
 
Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores.
Fraschini R, Beretta A, Sironi L, Musacchio A, Lucchini G, Piatti S.
EMBO J. 2001 Dec 3;20(23):6648-59.
PMID 11726501
 
Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events.
Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ.
J Cell Biol. 1998 Jun 15;141(6):1393-406.
PMID 9628895
 
CDC20, a potential cancer therapeutic target, is negatively regulated by p53.
Kidokoro T, Tanikawa C, Furukawa Y, Katagiri T, Nakamura Y, Matsuda K.
Oncogene. 2008 Mar 6;27(11):1562-71.
PMID 17873905
 
A new Mad2-interacting domain of Cdc20 is critical for the function of Mad2-Cdc20 complex in the spindle assembly checkpoint.
Mondal G, Baral RN, Roychoudhury S.
Biochem J. 2006 Jun 1;396(2):243-53.
PMID 16497171
 
Overexpression of Cdc20 leads to impairment of the spindle assembly checkpoint and aneuploidization in oral cancer.
Mondal G, Sengupta S, Panda CK, Gollin SM, Saunders WS, Roychoudhury S.
Carcinogenesis. 2007 Jan;28(1):81-92.
PMID 16777988
 
The spindle checkpoint: structural insights into dynamic signalling.
Musacchio A, Hardwick KG.
Nat Rev Mol Cell Biol. 2002 Oct;3(10):731-41. (Review)
PMID 12360190
 
Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.
Sironi L, Melixetian M, Faretta M, Prosperini E, Helin K, Musacchio A.
EMBO J. 2001 Nov 15;20(22):6371-82.
PMID 11707408
 
Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint.
Tang Z, Shu H, Oncel D, Chen S, Yu H.
Mol Cell. 2004 Nov 5;16(3):387-97.
PMID 15525512
 
Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1.
Weinstein J.
J Biol Chem. 1997 Nov 7;272(45):28501-11.
PMID 9353311
 
p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase.
Wu H, Lan Z, Li W, Wu S, Weinstein J, Sakamoto KM, Dai W.
Oncogene. 2000 Sep 21;19(40):4557-62
PMID 11030144
 
Cdc20: a WD40 activator for a cell cycle degradation machine.
Yu H.
Mol Cell. 2007 Jul 6;27(1):3-16.
PMID 17612486
 

Citation

This paper should be referenced as such :
Roychoudhury, S ; Banerjee, T ; Nath, S. CDC20 (cell division cycle 20 homolog (S
cerevisiae))
Atlas Genet Cytogenet Oncol Haematol. 2009;13(2):104-105.
Free journal version : [ pdf ]   [ DOI ]
On line version : http://AtlasGeneticsOncology.org/Genes/CDC20ID40003ch1p34.html


External links

Nomenclature
HGNC (Hugo)CDC20   1723
Cards
AtlasCDC20ID40003ch1p34
Entrez_Gene (NCBI)CDC20  991  cell division cycle 20
AliasesCDC20A; bA276H19.3; p55CDC
GeneCards (Weizmann)CDC20
Ensembl hg19 (Hinxton)ENSG00000117399 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000117399 [Gene_View]  chr1:43358955-43363202 [Contig_View]  CDC20 [Vega]
ICGC DataPortalENSG00000117399
TCGA cBioPortalCDC20
AceView (NCBI)CDC20
Genatlas (Paris)CDC20
WikiGenes991
SOURCE (Princeton)CDC20
Genetics Home Reference (NIH)CDC20
Genomic and cartography
GoldenPath hg38 (UCSC)CDC20  -     chr1:43358955-43363202 +  1p34.2   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)CDC20  -     1p34.2   [Description]    (hg19-Feb_2009)
EnsemblCDC20 - 1p34.2 [CytoView hg19]  CDC20 - 1p34.2 [CytoView hg38]
Mapping of homologs : NCBICDC20 [Mapview hg19]  CDC20 [Mapview hg38]
OMIM603618   
Gene and transcription
Genbank (Entrez)AF099644 AK225504 AK302539 AK303844 AK312780
RefSeq transcript (Entrez)NM_001255
RefSeq genomic (Entrez)
Consensus coding sequences : CCDS (NCBI)CDC20
Cluster EST : UnigeneHs.524947 [ NCBI ]
CGAP (NCI)Hs.524947
Alternative Splicing GalleryENSG00000117399
Gene ExpressionCDC20 [ NCBI-GEO ]   CDC20 [ EBI - ARRAY_EXPRESS ]   CDC20 [ SEEK ]   CDC20 [ MEM ]
Gene Expression Viewer (FireBrowse)CDC20 [ Firebrowse - Broad ]
SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
GenevestigatorExpression in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)991
GTEX Portal (Tissue expression)CDC20
Human Protein AtlasENSG00000117399-CDC20 [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ12834   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtQ12834  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProQ12834
Splice isoforms : SwissVarQ12834
PhosPhoSitePlusQ12834
Domaine pattern : Prosite (Expaxy)WD_REPEATS_1 (PS00678)    WD_REPEATS_2 (PS50082)    WD_REPEATS_REGION (PS50294)   
Domains : Interpro (EBI)Apc4_WD40_dom    CDC20    Cdc20/Fizzy    WD40/YVTN_repeat-like_dom    WD40_repeat    WD40_repeat_CS    WD40_repeat_dom   
Domain families : Pfam (Sanger)ANAPC4_WD40 (PF12894)    WD40 (PF00400)   
Domain families : Pfam (NCBI)pfam12894    pfam00400   
Domain families : Smart (EMBL)WD40 (SM00320)  
Conserved Domain (NCBI)CDC20
DMDM Disease mutations991
Blocks (Seattle)CDC20
PDB (SRS)4GGA    4GGC    4GGD    4N14    5G04    5KHR    5LCW   
PDB (PDBSum)4GGA    4GGC    4GGD    4N14    5G04    5KHR    5LCW   
PDB (IMB)4GGA    4GGC    4GGD    4N14    5G04    5KHR    5LCW   
PDB (RSDB)4GGA    4GGC    4GGD    4N14    5G04    5KHR    5LCW   
Structural Biology KnowledgeBase4GGA    4GGC    4GGD    4N14    5G04    5KHR    5LCW   
SCOP (Structural Classification of Proteins)4GGA    4GGC    4GGD    4N14    5G04    5KHR    5LCW   
CATH (Classification of proteins structures)4GGA    4GGC    4GGD    4N14    5G04    5KHR    5LCW   
SuperfamilyQ12834
Human Protein Atlas [tissue]ENSG00000117399-CDC20 [tissue]
Peptide AtlasQ12834
HPRD04686
IPIIPI00329526   IPI00909326   IPI00382768   
Protein Interaction databases
DIP (DOE-UCLA)Q12834
IntAct (EBI)Q12834
FunCoupENSG00000117399
BioGRIDCDC20
STRING (EMBL)CDC20
ZODIACCDC20
Ontologies - Pathways
QuickGOQ12834
Ontology : AmiGOspindle pole  protein binding  nucleoplasm  anaphase-promoting complex  centrosome  spindle  cytosol  cell cycle  sister chromatid cohesion  mitotic sister chromatid cohesion  protein C-terminus binding  positive regulation of cell proliferation  anaphase-promoting complex binding  protein deubiquitination  enzyme binding  anaphase-promoting complex-dependent catabolic process  anaphase-promoting complex-dependent catabolic process  positive regulation of synaptic plasticity  regulation of meiotic nuclear division  protein ubiquitination involved in ubiquitin-dependent protein catabolic process  histone deacetylase binding  proteasome-mediated ubiquitin-dependent protein catabolic process  protein complex  perinuclear region of cytoplasm  regulation of dendrite development  cell division  negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle  positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition  regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle  positive regulation of synapse maturation  mitotic spindle assembly  ubiquitin-protein transferase activator activity  positive regulation of ubiquitin protein ligase activity  
Ontology : EGO-EBIspindle pole  protein binding  nucleoplasm  anaphase-promoting complex  centrosome  spindle  cytosol  cell cycle  sister chromatid cohesion  mitotic sister chromatid cohesion  protein C-terminus binding  positive regulation of cell proliferation  anaphase-promoting complex binding  protein deubiquitination  enzyme binding  anaphase-promoting complex-dependent catabolic process  anaphase-promoting complex-dependent catabolic process  positive regulation of synaptic plasticity  regulation of meiotic nuclear division  protein ubiquitination involved in ubiquitin-dependent protein catabolic process  histone deacetylase binding  proteasome-mediated ubiquitin-dependent protein catabolic process  protein complex  perinuclear region of cytoplasm  regulation of dendrite development  cell division  negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle  positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition  regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle  positive regulation of synapse maturation  mitotic spindle assembly  ubiquitin-protein transferase activator activity  positive regulation of ubiquitin protein ligase activity  
Pathways : KEGGCell cycle    Oocyte meiosis    Ubiquitin mediated proteolysis    HTLV-I infection    Viral carcinogenesis   
REACTOMEQ12834 [protein]
REACTOME PathwaysR-HSA-983168 [pathway]   
NDEx NetworkCDC20
Atlas of Cancer Signalling NetworkCDC20
Wikipedia pathwaysCDC20
Orthology - Evolution
OrthoDB991
GeneTree (enSembl)ENSG00000117399
Phylogenetic Trees/Animal Genes : TreeFamCDC20
HOVERGENQ12834
HOGENOMQ12834
Homologs : HomoloGeneCDC20
Homology/Alignments : Family Browser (UCSC)CDC20
Gene fusions - Rearrangements
Tumor Fusion PortalCDC20
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerCDC20 [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)CDC20
dbVarCDC20
ClinVarCDC20
1000_GenomesCDC20 
Exome Variant ServerCDC20
ExAC (Exome Aggregation Consortium)ENSG00000117399
GNOMAD BrowserENSG00000117399
Genetic variants : HAPMAP991
Genomic Variants (DGV)CDC20 [DGVbeta]
DECIPHERCDC20 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisCDC20 
Mutations
ICGC Data PortalCDC20 
TCGA Data PortalCDC20 
Broad Tumor PortalCDC20
OASIS PortalCDC20 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICCDC20  [overview]  [genome browser]  [tissue]  [distribution]  
Mutations and Diseases : HGMDCDC20
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD - Leiden Open Variation Database
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
BioMutasearch CDC20
DgiDB (Drug Gene Interaction Database)CDC20
DoCM (Curated mutations)CDC20 (select the gene name)
CIViC (Clinical Interpretations of Variants in Cancer)CDC20 (select a term)
intoGenCDC20
NCG5 (London)CDC20
Cancer3DCDC20(select the gene name)
Impact of mutations[PolyPhen2] [SIFT Human Coding SNP] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
Diseases
OMIM603618   
Orphanet
DisGeNETCDC20
MedgenCDC20
Genetic Testing Registry CDC20
NextProtQ12834 [Medical]
TSGene991
GENETestsCDC20
Target ValidationCDC20
Huge Navigator CDC20 [HugePedia]
snp3D : Map Gene to Disease991
BioCentury BCIQCDC20
ClinGenCDC20
Clinical trials, drugs, therapy
Chemical/Protein Interactions : CTD991
Chemical/Pharm GKB GenePA26257
Clinical trialCDC20
Miscellaneous
canSAR (ICR)CDC20 (select the gene name)
Probes
Litterature
PubMed239 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
CoreMineCDC20
EVEXCDC20
GoPubMedCDC20
iHOPCDC20
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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