Atlas of Genetics and Cytogenetics in Oncology and Haematology
CDC20 (cell division cycle 20 homolog (S. cerevisiae))
| Identity |
| Other names | CDC20A |
| MGC102824 | |
| P55CDC-LSB | |
| bA276H19.3 | |
| p55CDC | |
| HGNC | CDC20 |
| Location | 1p34.1 |
| DNA/RNA |
| Description | Start: 43,597,199 bp from pter End: 43,601,461 bp from pter Size: 4,262 bases Orientation: plus strand 13 exons (Entrez), 15 Exons (Ensembl) |
| Transcription | 1697 bp |
| Pseudogene | No |
| Protein |
 | |
| The N-terminal amino acids from 1-153 contains most of the functional domains consisting of the Kinetochore binding domain, Mad2 binding domain and the Anaphase promoting complex (APC) activation domain. Amino acids 129-499 contain the WD-repeat region. | |
| Description | Size: 499 amino acids; 54723 Da. Subunit: Interacts with MAD2L1. The phosphorylated form interacts with APC/C. Developmental stage: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. |
| Expression | It is expressed in all cells, no tissue specificity. |
| Localisation | Nucleus |
| Function | CDC20 is a key player in the Spindle Assembly Checkpoint (SAC). When a cell is dividing mitotically, the Metaphase to Anaphase transition is stringently monitored by SAC. After proper alignment of all the sister chromatids to the spindle fibers during metaphase, the Mitotic Checkpoint Complex detaches from CDC20 and free CDC20 protein activates the Anaphase promoting Complex (APC) Activated APC can then degrade Securin which frees the protease Separase. Free Separase can now degrade the Cohesin molecules binding the two sister chromatids together. Upon degradation of Cohesin, the two sister chromatids are free and can migrate to the two spindle poles, thus, initiating Anaphase. |
| Homology | The C-terminal half is highly conserved from humans to yeast. |
| Mutations |
| Germinal | No |
| Somatic | No |
| Implicated in |
| Entity | Various tumors |
| Disease | There have been few reports of overexpression of CDC20 in various tumors for eg, greater than 3% of Cdc20 expression was found in bladder cancer, breast cancer, cervical cancer, cholangiocellular carcinoma, AML, CML, colon and rectum carcinoma, esophageal cancer, gastric cancer, gastric cancer (diffuse type), liver cancer, lung cancer (NSCLC), lung cancer (SCLC), osteosarcoma, pancreatic cancer, prostate cancer, renal carcinoma, soft tissue tumor, testicular tumor (Kidoko T, et al., 2007), head and neck cancer (Mondal G, et al., 2007). |
| Prognosis | No |
| Cytogenetics | No |
| Hybrid/Mutated Gene | No |
| Abnormal Protein | No |
| Oncogenesis | Overexpression of CDC20 has been observed in several tumor tissues. |
| External links |
| Bibliography |
| Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1. |
| Weinstein J. |
| J Biol Chem. 1997 Nov 7;272(45):28501-11. |
| PMID 9353311 |
| Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. |
| Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ. |
| J Cell Biol. 1998 Jun 15;141(6):1393-406. |
| PMID 9628895 |
| Cdc20 associates with the kinase aurora2/Aik. |
| Farruggio DC, Townsley FM, Ruderman JV. |
| Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7306-11. |
| PMID 10377410 |
| p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase. |
| Wu H, Lan Z, Li W, Wu S, Weinstein J, Sakamoto KM, Dai W. |
| Oncogene. 2000 Sep 21;19(40):4557-62 |
| PMID 11030144 |
| Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. |
| Fraschini R, Beretta A, Sironi L, Musacchio A, Lucchini G, Piatti S. |
| EMBO J. 2001 Dec 3;20(23):6648-59. |
| PMID 11726501 |
| Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint. |
| Sironi L, Melixetian M, Faretta M, Prosperini E, Helin K, Musacchio A. |
| EMBO J. 2001 Nov 15;20(22):6371-82. |
| PMID 11707408 |
| The spindle checkpoint: structural insights into dynamic signalling. |
| Musacchio A, Hardwick KG. |
| Nat Rev Mol Cell Biol. 2002 Oct;3(10):731-41. (Review) |
| PMID 12360190 |
| Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. |
| Tang Z, Shu H, Oncel D, Chen S, Yu H. |
| Mol Cell. 2004 Nov 5;16(3):387-97. |
| PMID 15525512 |
| A new Mad2-interacting domain of Cdc20 is critical for the function of Mad2-Cdc20 complex in the spindle assembly checkpoint. |
| Mondal G, Baral RN, Roychoudhury S. |
| Biochem J. 2006 Jun 1;396(2):243-53. |
| PMID 16497171 |
| Overexpression of Cdc20 leads to impairment of the spindle assembly checkpoint and aneuploidization in oral cancer. |
| Mondal G, Sengupta S, Panda CK, Gollin SM, Saunders WS, Roychoudhury S. |
| Carcinogenesis. 2007 Jan;28(1):81-92. |
| PMID 16777988 |
| Cdc20: a WD40 activator for a cell cycle degradation machine. |
| Yu H. |
| Mol Cell. 2007 Jul 6;27(1):3-16. |
| PMID 17612486 |
| CDC20, a potential cancer therapeutic target, is negatively regulated by p53. |
| Kidokoro T, Tanikawa C, Furukawa Y, Katagiri T, Nakamura Y, Matsuda K. |
| Oncogene. 2008 Mar 6;27(11):1562-71. |
| PMID 17873905 |
| REVIEW articles | automatic search in PubMed |
| Last year publications | automatic search in PubMed |
| Contributor(s) |
| Written | 04-2008 | Susanta Roychoudhury, Taraswi Banerjee, Somsubhra Nath |
| Human Genetics and Genomics Division, Indian Institute of Chemical Biology, Kolkata-700 032, India |
| Citation |
| This paper should be referenced as such : |
| Roychoudhury S, Banerjee T, Nath S . CDC20 (cell division cycle 20 homolog (S. cerevisiae)). Atlas Genet Cytogenet Oncol Haematol. April 2008 . URL : http://AtlasGeneticsOncology.org/Genes/CDC20ID40003ch1p34.html |
| © Atlas of Genetics and Cytogenetics in Oncology and Haematology | indexed on : Mon Aug 11 21:12:49 2008 |
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