CENPF (centromere protein F, 350/400ka (mitosin))
2008-04-01 Hideo Shigeishi , Koichiro Higashikawa , Nobuyuki Kamata AffiliationDepartment of Oral, Maxillofacial Surgery, Hiroshima University, Kasumi, Hiroshima 734-855, Japan
Identity
HGNC
LOCATION
1q41
LOCUSID
ALIAS
CENF,CILD31,PRO1779,STROMS,hcp-1
FUSION GENES
DNA/RNA
A schematic diagram of the CENPF gene. The exon numbers are labeled.
Description
The CENPF gene structure consists of twenty exons, ranging from 92 to 3,404 bp, and nineteen introns, ranging from approximately 1 to approximately 10 kb.
Transcription
10,294 bp mRNA; 9630 bp open reading frame.
Pseudogene
No pseudogene.
Proteins
A schematics representing the domain structure of full length CENPF. NLS, nuclear localization signal.
Description
The gene encodes a protein associated with the centromere-kinetochore complex, 3210 amino acids (aa), 367594 Da, containing internal repeats, coiled-coil (potential) and NLS (potential).
Expression
Breast, eye, gastro-intestinal tract, heart, liver, lymph node, ovary, placenta, skin, stomach, testis.
Localisation
Nucleus matrix, but not in the nucleolus, reorganization to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis.
Function
CenpF is recruited to kinetochore early in mitosis after recruitment of Bub1 and modulates kinetochore association of certain mitotic proteins including BubR1 for kinetochore assembly.
CenpF that has a CAAX motif in their C-terminal is target for farnesylation. This modification changes is necessary for CenpF function at the G2/M transition. CenpE and CenpF have a significant role in the antitumor activity of farnesyl transferase inhibitors due to their importance in normal cell division.
CenpF that has a CAAX motif in their C-terminal is target for farnesylation. This modification changes is necessary for CenpF function at the G2/M transition. CenpE and CenpF have a significant role in the antitumor activity of farnesyl transferase inhibitors due to their importance in normal cell division.
Homology
no
Implicated in
Entity name
Head and neck squamous cell carcinoma
Disease
CENPF gene amplification and overexpression were observed in head and neck squamous cell carcinoma (HNSCC). Up-regulation of CenpF, especially by gene amplification, suggests the possibility that increased CenpF protein levels could influence tumorigenesis particularly at early stages of tumor development. In addition, over-expression of CenpF is significantly correlated with poor prognosis of HNSCC. CenpF expression is able to use clinically as a proliferation marker in oral epithelia.
Entity name
Breast cancer
Disease
Over-expression of CENPF mRNA was associated with larger tumor size as well as estrogen receptor (ER) - negative, high grade tumors. CENPF mRNA expression correlated significantly with worse overall survival and a decreased probability of remaining metastasis-free. CenpF expression was also correlated with telomerase activity, cyclin E over-expression, c-Myc amplification and nuclear expression of surviving, indicating that CenpF is a good biomarker for proliferation of breast cancer. ver-expressing In addition, a significant proportion of brest cancer cells over-expressing CENPF were aneuploid, supporting evidence for the relation between CenpF expression and chromosomal instability.
Entity name
Astrocytic gliomas
Disease
In microarray and real-time RT-PCR analyses, CENPF mRNA levels significantly increased in primary astrocytic gliomas. Interestingly, secondary glioblastomas demonstrated higher CENPF mRNA levels than primary glioblastomas. However, amplification of the gene was not found.
Entity name
Salivary gland tumor
Disease
CenpF expression was significantly correlated with Ki-67 labeling index in primary malignant salivary gland tumor by immunohistochemical study. In addition, CENPF mRNA level was associated with clinical stage. The data suggests that CenpF expression is a candidate for biomarker of proliferation of salivary tumor.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 11302861 | 2001 | Cancer and autoimmunity: autoimmune and rheumatic features in patients with malignancies. | Abu-Shakra M et al |
| 10852915 | 2000 | Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules. | Ashar HR et al |
| 11640891 | 2001 | A multistep model for paclitaxel-induced apoptosis in human breast cancer cell lines. | Blajeski AL et al |
| 16252009 | 2005 | Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F. | Bomont P et al |
| 17389228 | 2007 | Bub1 is essential for assembly of the functional inner centromere. | Boyarchuk Y et al |
| 9763420 | 1998 | Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1. | Chan GK et al |
| 11154688 | 2001 | The farnesyltransferase inhibitor, FTI-2153, blocks bipolar spindle formation and chromosome alignment and causes prometaphase accumulation during mitosis of human lung cancer cells. | Crespo NC et al |
| 9499420 | 1998 | Localization of motor-related proteins and associated complexes to active, but not inactive, centromeres. | Faulkner NE et al |
| 16219694 | 2005 | Silencing Cenp-F weakens centromeric cohesion, prevents chromosome alignment and activates the spindle checkpoint. | Holt SV et al |
| 17485487 | 2007 | Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments. | Huang H et al |
| 9914370 | 1998 | The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis. | Jablonski SA et al |
| 15020684 | 2004 | Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression. | Johnson VL et al |
| 15062103 | 2004 | The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo. | Joseph J et al |
| 8875058 | 1996 | Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies. | Landberg G et al |
| 15654331 | 2005 | FoxM1 is required for execution of the mitotic programme and chromosome stability. | Laoukili J et al |
| 7542657 | 1995 | CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. | Liao H et al |
| 10745166 | 2000 | Markers of proliferation in normal and leukoplakic oral epithelia. | Liu SC et al |
| 12640463 | 2003 | Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis. | Liu ST et al |
| 8844395 | 1996 | Dynamic continuity of nuclear and mitotic matrix proteins in the cell cycle. | Mancini MA et al |
| 17205517 | 2007 | CENP-F expression is associated with poor prognosis and chromosomal instability in patients with primary breast cancer. | O'Brien SL et al |
| 16230362 | 2005 | Recent advances in understanding the antineoplastic mechanisms of farnesyltransferase inhibitors. | Pan J et al |
| 9336656 | 1997 | High frequency of neoplasia in patients with autoantibodies to centromere protein CENP-F. | Rattner JB et al |
| 10607828 | 2000 | Human centromeres and neocentromeres show identical distribution patterns of >20 functionally important kinetochore-associated proteins. | Saffery R et al |
| 15339662 | 2004 | Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis. | Salic A et al |
| 17431110 | 2007 | Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function. | Schafer-Hales K et al |
| 15927522 | 2005 | Correlation of CENP-F gene expression with tumor-proliferating activity in human salivary gland tumors. | Shigeishi H et al |
| 11003657 | 2000 | Nonperiodic activity of the human anaphase-promoting complex-Cdh1 ubiquitin ligase results in continuous DNA synthesis uncoupled from mitosis. | Sorensen CS et al |
| 15133482 | 2004 | Sgt1 is required for human kinetochore assembly. | Steensgaard P et al |
| 16565862 | 2006 | Cenp-F (mitosin) is more than a mitotic marker. | Varis A et al |
| 17600710 | 2007 | Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule motor complexes. | Vergnolle MA et al |
| 15870278 | 2005 | Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death. | Yang Z et al |
| 15494374 | 2004 | GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation. | Yang Z et al |
| 16233975 | 2005 | The kinetochore and cancer: what's the connection? | Yuen KW et al |
| 7642639 | 1995 | The C terminus of mitosin is essential for its nuclear localization, centromere/kinetochore targeting, and dimerization. | Zhu X et al |
| 11303627 | 2001 | CENP-F gene amplification and overexpression in head and neck squamous cell carcinomas. | de la Guardia C et al |
| 12937144 | 2003 | Characterization of gene expression profiles associated with glioma progression using oligonucleotide-based microarray analysis and real-time reverse transcription-polymerase chain reaction. | van den Boom J et al |
Other Information
Locus ID:
NCBI: 1063
MIM: 600236
HGNC: 1857
Ensembl: ENSG00000117724
Variants:
dbSNP: 1063
ClinVar: 1063
TCGA: ENSG00000117724
COSMIC: CENPF
RNA/Proteins
| Gene ID | Transcript ID | Uniprot |
|---|---|---|
| ENSG00000117724 | ENST00000366955 | P49454 |
| ENSG00000117724 | ENST00000614578 | A0A087WTY4 |
Expression (GTEx)
Pathways
Protein levels (Protein atlas)
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 37287300 | 2024 | CENPF Upregulation is Associated with Immunosuppressive Status and Poor Clinical Outcomes in Lung Adenocarcinoma Validated by qRT-PCR. | 0 |
| 37287300 | 2024 | CENPF Upregulation is Associated with Immunosuppressive Status and Poor Clinical Outcomes in Lung Adenocarcinoma Validated by qRT-PCR. | 0 |
| 36720923 | 2023 | CENPF knockdown inhibits adriamycin chemoresistance in triple-negative breast cancer via the Rb-E2F1 axis. | 0 |
| 36899270 | 2023 | Long Non-Coding RNA Small Nucleolar RNA Host Gene 4 Induced by Transcription Factor SP1 Promoted the Progression of Nasopharyngeal Carcinoma Through Modulating microRNA-510-5p/Centromere Protein F Axis. | 1 |
| 37247093 | 2023 | Integrated pan-cancer analysis of centromere protein F and experimental verification of its role and clinical significance in cholangiocarcinoma. | 0 |
| 37814871 | 2023 | [Heterogeneity analysis of pancreatic cancer and identification of molecular subtypes of tumor cells based on CEACAM5, LGALS1 and CENPF gene expression]. | 0 |
| 38002928 | 2023 | Novel Loss of Function Variants in CENPF Including a Large Intragenic Deletion in Patients with Strømme Syndrome. | 0 |
| 36720923 | 2023 | CENPF knockdown inhibits adriamycin chemoresistance in triple-negative breast cancer via the Rb-E2F1 axis. | 0 |
| 36899270 | 2023 | Long Non-Coding RNA Small Nucleolar RNA Host Gene 4 Induced by Transcription Factor SP1 Promoted the Progression of Nasopharyngeal Carcinoma Through Modulating microRNA-510-5p/Centromere Protein F Axis. | 1 |
| 37247093 | 2023 | Integrated pan-cancer analysis of centromere protein F and experimental verification of its role and clinical significance in cholangiocarcinoma. | 0 |
| 37814871 | 2023 | [Heterogeneity analysis of pancreatic cancer and identification of molecular subtypes of tumor cells based on CEACAM5, LGALS1 and CENPF gene expression]. | 0 |
| 38002928 | 2023 | Novel Loss of Function Variants in CENPF Including a Large Intragenic Deletion in Patients with Strømme Syndrome. | 0 |
| 34857915 | 2022 | Centromere protein F promotes progression of hepatocellular carcinoma through ERK and cell cycle-associated pathways. | 8 |
| 35123514 | 2022 | CENPF/CDK1 signaling pathway enhances the progression of adrenocortical carcinoma by regulating the G2/M-phase cell cycle. | 13 |
| 35189004 | 2022 | CENPF as an independent prognostic and metastasis biomarker corresponding to CD4+ memory T cells in cutaneous melanoma. | 15 |
Citation
Hideo Shigeishi ; Koichiro Higashikawa ; Nobuyuki Kamata
CENPF (centromere protein F, 350/400ka (mitosin))
Atlas Genet Cytogenet Oncol Haematol. 2008-04-01
Online version: http://atlasgeneticsoncology.org/gene/40057/cenpf
