| Description | 693 amino acids; 76 kDa;there are two variant forms of human GMP synthetase; homodimerization; GMP synthetase contains two functional domains: a glutamine amidotransferase (glutaminase domain, with a conserved Cys-His-Glu triad), responsible for glutamine hydrolysis, and a synthetase domain; responsible for ATP hydrolysis and GMP formation |
| Expression | higher in proliferating, transformed cells than in nontransformed cells; in normal cells, higher expression in fibroblasts, followed by bone marrow, leukocytes, erythrocytes, placenta, and liver |
| Localisation | cytoplasmic |
| Function | enzyme of the de novo synthesis of guanine nucleotides: amidotransferase that catalyzes the amination of xanthosine 5 prime monophosphate to form GMP in the presence of ATP and glutamine; GTP is also involved in many enzymatic reactions important for cell division. |
| Human GMP synthetase. |
| Page T, Bakay B, Nyhan WL |
| The International journal of biochemistry. 1984 ; 16 (1) : 117-120. |
| PMID 6698284 |
| |
| Human GMP synthetase. Protein purification, cloning, and functional expression of cDNA. |
| Hirst M, Haliday E, Nakamura J, Lou L |
| The Journal of biological chemistry. 1994 ; 269 (38) : 23830-23837. |
| PMID 8089153 |
| |
| High-level production from a baculovirus expression system and biochemical characterization of human GMP synthetase. |
| Lou L, Nakamura J, Tsing S, Nguyen B, Chow J, Straub K, Chan H, Barnett J |
| Protein expression and purification. 1995 ; 6 (4) : 487-495. |
| PMID 8527935 |
| |
| Biochemical characterization of human GMP synthetase. |
| Nakamura J, Lou L |
| The Journal of biological chemistry. 1995 ; 270 (13) : 7347-7353. |
| PMID 7706277 |
| |
| The glutamine hydrolysis function of human GMP synthetase. Identification of an essential active site cysteine. |
| Nakamura J, Straub K, Wu J, Lou L |
| The Journal of biological chemistry. 1995 ; 270 (40) : 23450-23455. |
| PMID 7559506 |
| |
| The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families. |
| Tesmer JJ, Klem TJ, Deras ML, Davisson VJ, Smith JL |
| Nature structural biology. 1996 ; 3 (1) : 74-86. |
| PMID 8548458 |
| |
| Assignment and ordering of twenty-three unique NotI-linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3. |
| Fedorova L, Kost-Alimova M, Gizatullin RZ, Alimov A, Zabarovska VI, Szeles A, Protopopov AI, Vorobieva NV, Kashuba VI, Klein G, Zelenin AV, Sheer D, Zabarovsky ER |
| European journal of human genetics : EJHG. 1997 ; 5 (2) : 110-116. |
| PMID 9195163 |
| |
| t(3;11)(q25;q23) fuses MLL with the GMPS (guanosine 5'-monophosphate synthetase) gene in treatment-related acute myeloid leukemia (AML). |
| Pegram LD, Megonigal MD, Lange BJ, Nowell PC, Rappaport EF, Felix CA |
| Blood. 1999 ; 94 (numero Suppl 1). |
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