Atlas of Genetics and Cytogenetics in Oncology and Haematology


Home   Genes   Leukemias   Solid Tumours   Cancer-Prone   Deep Insight   Case Reports   Journals  Portal   Teaching   

X Y 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 NA

TFF3

Identity

Other namesITF (Intestinal Trefoil Factor)
Hugo TFF3
Location 21q22.3
Local_order Belongs to the TFF cluster

DNA/RNA

Description 3.3 kb gene, 3 exons
Transcription 600 bp

Protein

Description precursor 74 amino acids, mature peptide 51 aminoacids, trefoil domain from aminoacids residues 24 to 67. The 74 aminoacids TFF3 protein precusor contains a signal peptide. The mature secreted peptide of 51 aminoacids contains one TFF (TreFoil Factor) domain and one acidic C-terminal domain. The acidic C-terminal domain contains a free cystein residue that promotes homodimerization and heterodimerization.
Expression Under normal conditions TFF3 is expressed by gobblet cell of the intesine and the colon. TFF3 expression was found in human respiratory tract, human conjunctival goblet cells and in human salivarygland. In addition TFF3 peptive was found in human hypothalamus.
Localisation In secreterory epithelia TFF3 is expressed by mucin-producing cells. In the brain TFF3 is expressed by a population of neurons of the human hypothamic paraventricular and supraoptic nuclei.
Function TFF3 promotes migration of epithelial cells in vitro and enhance mucosal healing and epithelial restitutions in vivo in the gastrointestinal mucosa. TFF3 deficient mice aresuceptible to colonic injury induced by standard agents and restitution is impaired. In addition TFF3 deficient mice have an increase in colonocyte apoptosis. The protective action of TFF3 involves activation of both EGF-R and PI3K-Akt pathways. The role of TFF3 in the brain is not clear yet.
Homology TFF3 belongs to the Trefoil peptide family (TFF) and possesse one TFF1 motif homologous to the TFF motif of TFF1 and TFF2. The TFF motif spans about 40 amino acids and is formed by 6 conserved cysteines residues involved in specific disulfites bridges.

Implicated in

Entity Inflamatory bowel diseases, TFF3 is expressed in cancer cells derived from the gastrointestinal tract, in hepatocellular carcinoma cells and in small cell lung carcinoma cells. Like TFF1, TFF3 is expressed in oestrogen-responsive breast cancer cell line and is expressed in breast cancer.
  

External links

Nomenclature
HugoTFF3
GDBTFF3
Entrez_GeneTFF3  7033  trefoil factor 3 (intestinal)
Cards
AtlasTFF3ID265
GeneCardsTFF3
EnsemblTFF3 [Search_View]   ENSG00000160180 [Gene_View]
GenatlasTFF3
GeneLynxTFF3
eGenomeTFF3
euGene7033
Genomic and cartography
GoldenPathTFF3  -  21q22.3   chr21:42605233-42608775 -  21q22.3   [Description]    (hg18-Mar_2006)
EnsemblTFF3 - 21q22.3 [CytoView]
NCBIMapview
OMIMDisease map [OMIM]
HomoloGeneTFF3
Gene and transcription
GenbankAF432265 [ ENTREZ ]
GenbankBC017859 [ ENTREZ ]
GenbankBQ016069 [ ENTREZ ]
GenbankDQ891963 [ ENTREZ ]
GenbankDQ895153 [ ENTREZ ]
RefSeqNM_003226 [ SRS ]    NM_003226 [ ENTREZ ]
RefSeqAC_000064 [ SRS ]    AC_000064 [ ENTREZ ]
RefSeqNC_000021 [ SRS ]    NC_000021 [ ENTREZ ]
RefSeqNT_030188 [ SRS ]    NT_030188 [ ENTREZ ]
RefSeqNW_927384 [ SRS ]    NW_927384 [ ENTREZ ]
AceViewTFF3 AceView - NCBI
UnigeneHs.82961 [ SRS ]    Hs.82961 [ NCBI ]     HS82961 [ spliceNest ]
Fast-db3395 (alternative variants)
Protein : pattern, domain, 3D structure
SwissProtQ07654 [ SRS]    Q07654 [ EXPASY ]     Q07654 [ INTERPRO ]
PrositePS00025 P_TREFOIL [ SRS ]    PS00025 P_TREFOIL [ Expasy ]
InterproIPR000519 P_trefoil [ SRS ]    IPR000519 P_trefoil [ EBI ]
CluSTrQ07654
PfamPF00088 Trefoil [ SRS ]    PF00088 Trefoil [ Sanger ]    pfam00088 [ NCBI-CDD ]
SmartSM00018 PD [EMBL]
BlocksQ07654
PDB1E9T [ SRS ]    1E9T [ PdbSum ],   1E9T [ IMB ]   1E9T [ RSDB ]
PDB1PE3 [ SRS ]    1PE3 [ PdbSum ],   1PE3 [ IMB ]   1PE3 [ RSDB ]
HPRD02797
Protein Interaction databases
DIPQ07654
IntActQ07654
Polymorphism : SNP, mutations, diseases
OMIM600633    [ map ]   
GENECLINICS600633
SNPTFF3 [dbSNP-NCBI]  
SNPNM_003226 [SNP-NCI]  
SNPTFF3 [GeneSNPs - Utah]  TFF3] [HGBASE - SRS]
HAPMAPTFF3 [HAPMAP]  
HGMDTFF3
General knowledge
Family BrowserTFF3 [UCSC Family Browser]
SOURCENM_003226
SMDHs.82961
SAGEHs.82961
GOextracellular region [Amigo]  extracellular region
GOdefense response [Amigo]  defense response
GOdigestion [Amigo]  digestion
GOsecretory granule [Amigo]  secretory granule
BIOCARTATrefoil Factors Initiate Mucosal Healing    [Genes]
PubGeneTFF3
TreeFamTFF3
CTD7033 [Comparative ToxicoGenomics Database]
Other databases
Probes
ProbeTFF3 Related clones (RZPD - Berlin)
PubMed
PubMed41 Pubmed reference(s) in LocusLink

Bibliography

hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus.
Hauser F, Poulsom R, Chinery R, Rogers LA, Hanby AM, Wright NA, Hoffmann W
Proceedings of the National Academy of Sciences of the United States of America. 1993 ; 90 (15) : 6961-6965.
PMID 8346203
 
Physiologic concentrations of glucose regulate fatty acid synthase activity in HepG2 cells by mediating fatty acid synthase mRNA stability.
Semenkovich CF, Coleman T, Goforth R
The Journal of biological chemistry. 1993 ; 268 (10) : 6961-6970.
PMID 8463228
 
Characterisation of the single copy trefoil peptides intestinal trefoil factor and pS2 and their ability to form covalent dimers.
Chinery R, Bates PA, De A, Freemont PS
FEBS letters. 1995 ; 357 (1) : 50-54.
PMID 8001677
 
Trefoil peptide protection of intestinal epithelial barrier function: cooperative interaction with mucin glycoprotein.
Kindon H, Pothoulakis C, Thim L, Lynch-Devaney K, Podolsky DK
Gastroenterology. 1995 ; 109 (2) : 516-523.
PMID 7615201
 
Oral trefoil peptides protect against ethanol- and indomethacin-induced gastric injury in rats.
Babyatsky MW, deBeaumont M, Thim L, Podolsky DK
Gastroenterology. 1996 ; 110 (2) : 489-497.
PMID 8566596
 
Impaired defense of intestinal mucosa in mice lacking intestinal trefoil factor.
Mashimo H, Wu DC, Podolsky DK, Fishman MC
Science (New York, N.Y.). 1996 ; 274 (5285) : 262-265.
PMID 8824194
 
The trefoil peptide family.
Sands BE, Podolsky DK
Annual review of physiology. 1996 ; 58 : 253-273.
PMID 8815795
 
Expression of human intestinal trefoil factor in malignant cells and its regulation by oestrogen in breast cancer cells.
May FE, Westley BR
The Journal of pathology. 1997 ; 182 (4) : 404-413.
PMID 9306961
 
Intestinal trefoil factor (TFF 3) and pS2 (TFF 1), but not spasmolytic polypeptide (TFF 2) mRNAs are co-expressed in normal, hyperplastic, and neoplastic human breast epithelium.
Poulsom R, Hanby AM, Lalani EN, Hauser F, Hoffmann W, Stamp GW
The Journal of pathology. 1997 ; 183 (1) : 30-38.
PMID 9370944
 
The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3.
Seib T, Blin N, Hilgert K, Seifert M, Theisinger B, Engel M, Dooley S, Zang KD, Welter C
Genomics. 1997 ; 40 (1) : 200-202.
PMID 9070946
 
Trefoil peptides: from structure to function.
Thim L
Cellular and molecular life sciences : CMLS. 1997 ; 53 (11-12) : 888-903.
PMID 9447240
 
Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells.
Efstathiou JA, Noda M, Rowan A, Dixon C, Chinery R, Jawhari A, Hattori T, Wright NA, Bodmer WF, Pignatelli M
Proceedings of the National Academy of Sciences of the United States of America. 1998 ; 95 (6) : 3122-3127.
PMID 9501226
 
Intestinal trefoil factor induces inactivation of extracellular signal-regulated protein kinase in intestinal epithelial cells.
Kanai M, Mullen C, Podolsky DK
Proceedings of the National Academy of Sciences of the United States of America. 1998 ; 95 (1) : 178-182.
PMID 9419349
 
Secretory peptides TFF1 and TFF3 synthesized in human conjunctival goblet cells.
Langer G, Jagla W, Behrens-Baumann W, Walter S, Hoffmann W
Investigative ophthalmology & visual science. 1999 ; 40 (10) : 2220-2224.
PMID 10476786
 
Localization of TFF3, a new mucus-associated peptide of the human respiratory tract.
Wiede A, Jagla W, Welte T, Kˆhnlein T, Busk H, Hoffmann W
American journal of respiratory and critical care medicine. 1999 ; 159 (4 Pt 1) : 1330-1335.
PMID 10194185
 
Co-localization of TFF3 peptide and oxytocin in the human hypothalamus.
Jagla W, Wiede A, Dietzmann K, Rutkowski K, Hoffmann W
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2000 ; 14 (9) : 1126-1131.
PMID 10834934
 
Distinct pathways of cell migration and antiapoptotic response to epithelial injury: structure-function analysis of human intestinal trefoil factor.
Kinoshita K, Taupin DR, Itoh H, Podolsky DK
Molecular and cellular biology. 2000 ; 20 (13) : 4680-4690.
PMID 10848594
 
Mechanisms of regulatory peptide action in the gastrointestinal tract: trefoil peptides.
Podolsky DK
Journal of gastroenterology. 2000 ; 35 Suppl 12 : 69-74.
PMID 10779222
 
Intestinal trefoil factor confers colonic epithelial resistance to apoptosis.
Taupin DR, Kinoshita K, Podolsky DK
Proceedings of the National Academy of Sciences of the United States of America. 2000 ; 97 (2) : 799-804.
PMID 10639160
 
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

Search in all EBI   NCBI

Contributor(s)

Written03-2001Catherine Tomasetto

Citation

This paper should be referenced as such :
Tomasetto C . TFF3. Atlas Genet Cytogenet Oncol Haematol. March 2001 .
URL : http://AtlasGeneticsOncology.org/Genes/TFF3ID265.html

© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Wed Jul 2 08:27:36 2008


Home   Genes   Leukemias   Solid Tumours   Cancer-Prone   Deep Insight   Case Reports   Journals  Portal   Teaching   

For comments and suggestions or contributions, please contact us

j.l.huret@chu-poitiers.fr.