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ERCC4 (xeroderma pigmentosum, complementation group F)

Written2001-05Anne Stary, Alain Sarasin
Laboratory of Genetic Instability, Cancer, UPR2169 CNRS, Institut de Recherches sur le Cancer, 7, rue guy Moquet, BP 8, 94801 VILLEJUIF, France

(Note : for Links provided by Atlas : click)


excision repair cross-complementing rodent repair deficiency, complementation group 4
excision repair cross-complementation group 4
Alias_symbol (synonym)RAD1
Other aliasXPF (xeroderma pigmentosum, complementation group F)
LocusID (NCBI) 2072
Atlas_Id 299
Location 16p13.12  [Link to chromosome band 16p13]
Location_base_pair Starts at 13920157 and ends at 13952348 bp from pter ( according to hg19-Feb_2009)  [Mapping ERCC4.png]
  XPF (16p13) - Courtesy Mariano Rocchi, Resources for Molecular Cytogenetics.
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)
FAM213A (10q23.1) / ERCC4 (16p13.12)


Description 28.2 kb
Transcription 2881 bp =D0 11 exons


  • xeroderma pigmentosum group F complementing factor; DNA-repair protein complementing XPF cells 905 amino acids; form a stable complex with the ERCC1 protein; The XPF protein and the ERCC1 protein form a complex that exhibits structure specific endonuclease activity that is responsible for the 5' incision during the NER reaction. XPF-ERCC1 also binds to XPA (through ERCC1) and to RPA (through XPF) but not preferentially to damaged DNA. At the site of a lesion Nucleotide Excision Repair (NER) proteins create a DNA bubble structure over a length of approximately 25 nucleotides and the XPG protein incises the damaged DNA strand 0-2 nucleotides 3' to the ssDNA-dsDNA junction. In most studies the 3'-incision made by the XPG protein appeared to be made prior to and independently of the 5'-incision by XPF-ERCC1. XP-F patients have a relatively mild XP phenotype without neurological abnormalities. Cells from XP-F patients are slightly UV-sensitive and exhibit low levels of repair initially after UV-irradiation.
  • The XPF protein is a single-stranded DNA endonuclease that is also involved in a pathway of recombination repair of DNA interstrand crosslinks.
  • Homology Substantial homology with the eucaryotic DNA repair and recombination proteins MEI-9 (Drosophila melanogaster), Rad16 (Saccharomyces cerevisae) and Rad1 (Schizosaccharomyces pombe).


    Germinal 9 point mutations ; 3 small deletions ; 1 small insertion ; 1 gross deletion

    Implicated in

    Entity xeroderma pigmentosum, XP group F
    Disease Early skin tumours in XPF patients


    Mammalian DNA nucleotide excision repair reconstituted with purified protein components.
    Aboussekhra A, Biggerstaff M, Shivji MK, Vilpo JA, Moncollin V, Podust VN, Protić M, Hübscher U, Egly JM, Wood RD
    Cell. 1995 ; 80 (6) : 859-868.
    PMID 7697716
    Nucleotide excision repair of DNA with recombinant human proteins: definition of the minimal set of factors, active forms of TFIIH, and modulation by CAK.
    Araújo SJ, Tirode F, Coin F, Pospiech H, Syväoja JE, Stucki M, Hübscher U, Egly JM, Wood RD
    Genes & development. 2000 ; 14 (3) : 349-359.
    PMID 10673506
    Reconstitution of human excision nuclease with recombinant XPF-ERCC1 complex.
    Bessho T, Sancar A, Thompson LH, Thelen MP
    The Journal of biological chemistry. 1997 ; 272 (6) : 3833-3837.
    PMID 9013642
    Co-correction of the ERCC1, ERCC4 and xeroderma pigmentosum group F DNA repair defects in vitro.
    Biggerstaff M, Szymkowski DE, Wood RD
    The EMBO journal. 1993 ; 12 (9) : 3685-3692.
    PMID 8253090
    Excision-repair patch lengths are similar for transcription-coupled repair and global genome repair in UV-irradiated human cells.
    Bowman KK, Smith CA, Hanawalt PC
    Mutation research. 1997 ; 385 (2) : 95-105.
    PMID 9447231
    ERCC4 (XPF) encodes a human nucleotide excision repair protein with eukaryotic recombination homologs.
    Brookman KW, Lamerdin JE, Thelen MP, Hwang M, Reardon JT, Sancar A, Zhou ZQ, Walter CA, Parris CN, Thompson LH
    Molecular and cellular biology. 1996 ; 16 (11) : 6553-6562.
    PMID 8887684
    Genetic polymorphisms in DNA repair genes and risk of lung cancer.
    Butkiewicz D, Rusin M, Enewold L, Shields PG, Chorazy M, Harris CC
    Carcinogenesis. 2001 ; 22 (4) : 593-597.
    PMID 11285194
    Mechanism of open complex and dual incision formation by human nucleotide excision repair factors.
    Evans E, Moggs JG, Hwang JR, Egly JM, Wood RD
    The EMBO journal. 1997 ; 16 (21) : 6559-6573.
    PMID 9351836
    Activity of individual ERCC1 and XPF subunits in DNA nucleotide excision repair.
    Gaillard PH, Wood RD
    Nucleic acids research. 2001 ; 29 (4) : 872-879.
    PMID 11160918
    ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines.
    Hayashi T, Takao M, Tanaka K, Yasui A
    Mutation research. 1998 ; 407 (3) : 269-276.
    PMID 9653453
    Action of DNA repair endonuclease ERCC1/XPF in living cells.
    Houtsmuller AB, Rademakers S, Nigg AL, Hoogstraten D, Hoeijmakers JH, Vermeulen W
    Science (New York, N.Y.). 1999 ; 284 (5416) : 958-961.
    PMID 10320375
    Late onset of skin cancers in 2 xeroderma pigmentosum group F siblings and a review of 30 Japanese xeroderma pigmentosum patients in groups D, E and F.
    Kondo S, Mamada A, Miyamoto C, Keong CH, Satoh Y, Fujiwara Y
    Photo-dermatology. 1989 ; 6 (2) : 89-95.
    PMID 2664729
    Regional mapping of human DNA excision repair gene ERCC4 to chromosome 16p13.13-p13.2.
    Liu P, Siciliano J, White B, Legerski R, Callen D, Reeders S, Siciliano MJ, Thompson LH
    Mutagenesis. 1993 ; 8 (3) : 199-205.
    PMID 8332082
    Characterization of molecular defects in xeroderma pigmentosum group F in relation to its clinically mild symptoms.
    Matsumura Y, Nishigori C, Yagi T, Imamura S, Takebe H
    Human molecular genetics. 1998 ; 7 (6) : 969-974.
    PMID 9580660
    Replication protein A confers structure-specific endonuclease activities to the XPF-ERCC1 and XPG subunits of human DNA repair excision nuclease.
    Matsunaga T, Park CH, Bessho T, Mu D, Sancar A
    The Journal of biological chemistry. 1996 ; 271 (19) : 11047-11050.
    PMID 8626644
    Domain mapping of the DNA binding, endonuclease, and ERCC1 binding properties of the human DNA repair protein XPF.
    McCutchen-Maloney SL, Giannecchini CA, Hwang MH, Thelen MP
    Biochemistry. 1999 ; 38 (29) : 9417-9425.
    PMID 10413517
    A case of xeroderma pigmentosum complementation group F with neurological abnormalities.
    Moriwaki S, Nishigori C, Imamura S, Yagi T, Takahashi C, Fujimoto N, Takebe H
    The British journal of dermatology. 1993 ; 128 (1) : 91-94.
    PMID 8427828
    Reaction mechanism of human DNA repair excision nuclease.
    Mu D, Hsu DS, Sancar A
    The Journal of biological chemistry. 1996 ; 271 (14) : 8285-8294.
    PMID 8626523
    ERCC1/ERCC4 5'-endonuclease activity as a determinant of hypoxic cell radiosensitivity.
    Murray D, Macann A, Hanson J, Rosenberg E
    International journal of radiation biology. 1996 ; 69 (3) : 319-327.
    PMID 8613681
    Xeroderma pigmentosum patients belonging to complementation group F and efficient liquid-holding recovery of ultraviolet damage.
    Nishigori C, Fujisawa H, Uyeno K, Kawaguchi T, Takebe H
    Photodermatology, photoimmunology & photomedicine. 1991 ; 8 (4) : 146-150.
    PMID 1814424
    Terminally differentiated human neurons repair transcribed genes but display attenuated global DNA repair and modulation of repair gene expression.
    Nouspikel T, Hanawalt PC
    Molecular and cellular biology. 2000 ; 20 (5) : 1562-1570.
    PMID 10669734
    Purification and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease.
    Park CH, Bessho T, Matsunaga T, Sancar A
    The Journal of biological chemistry. 1995 ; 270 (39) : 22657-22660.
    PMID 7559382
    Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins.
    Park CH, Sancar A
    Proceedings of the National Academy of Sciences of the United States of America. 1994 ; 91 (11) : 5017-5021.
    PMID 8197175
    Human chromosome 15 confers partial complementation of phenotypes to xeroderma pigmentosum group F cells.
    Saxon PJ, Schultz RA, Stanbridge EJ, Friedberg EC
    American journal of human genetics. 1989 ; 44 (4) : 474-485.
    PMID 2929593
    Homozygous R788W point mutation in the XPF gene of a patient with xeroderma pigmentosum and late-onset neurologic disease.
    Sijbers AM, van Voorst Vader PC, Snoek JW, Raams A, Jaspers NG, Kleijer WJ
    The Journal of investigative dermatology. 1998 ; 110 (5) : 832-836.
    PMID 9579555
    Molecular cloning of the human nucleotide-excision-repair gene ERCC4.
    Thompson LH, Brookman KW, Weber CA, Salazar EP, Reardon JT, Sancar A, Deng Z, Siciliano MJ
    Proceedings of the National Academy of Sciences of the United States of America. 1994 ; 91 (15) : 6855-6859.
    PMID 8041709
    The non-catalytic function of XPG protein during dual incision in human nucleotide excision repair.
    Wakasugi M, Reardon JT, Sancar A
    The Journal of biological chemistry. 1997 ; 272 (25) : 16030-16034.
    PMID 9188507
    Disruption of mouse ERCC1 results in a novel repair syndrome with growth failure, nuclear abnormalities and senescence.
    Weeda G, Donker I, de Wit J, Morreau H, Janssens R, Vissers CJ, Nigg A, van Steeg H, Bootsma D, Hoeijmakers JH
    Current biology : CB. 1997 ; 7 (6) : 427-439.
    PMID 9197240
    Novel functional interactions between nucleotide excision DNA repair proteins influencing the enzymatic activities of TFIIH, XPG, and ERCC1-XPF.
    Winkler GS, Sugasawa K, Eker AP, de Laat WL, Hoeijmakers JH
    Biochemistry. 2001 ; 40 (1) : 160-165.
    PMID 11141066
    DNA damage recognition during nucleotide excision repair in mammalian cells.
    Wood RD
    Biochimie. 1999 ; 81 (1-2) : 39-44.
    PMID 10214908
    Sensitivity of group F xeroderma pigmentosum cells to UV and mitomycin C relative to levels of XPF and ERCC1 overexpression.
    Yagi T, Katsuya A, Koyano A, Takebe H
    Mutagenesis. 1998 ; 13 (6) : 595-599.
    PMID 9862190
    Complete restoration of normal DNA repair characteristics in group F xeroderma pigmentosum cells by over-expression of transfected XPF cDNA.
    Yagi T, Matsumura Y, Sato M, Nishigori C, Mori T, Sijbers AM, Takebe H
    Carcinogenesis. 1998 ; 19 (1) : 55-60.
    PMID 9472693
    Analysis of point mutations in an ultraviolet-irradiated shuttle vector plasmid propagated in cells from Japanese xeroderma pigmentosum patients in complementation groups A and F.
    Yagi T, Tatsumi-Miyajima J, Sato M, Kraemer KH, Takebe H
    Cancer research. 1991 ; 51 (12) : 3177-3182.
    PMID 2039995
    A low content of ERCC1 and a 120 kDa protein is a frequent feature of group F xeroderma pigmentosum fibroblast cells.
    Yagi T, Wood RD, Takebe H
    Mutagenesis. 1997 ; 12 (1) : 41-44.
    PMID 9025096
    Xeroderma pigmentosum fibroblasts are more sensitive to asbestos fibers than are normal human fibroblasts.
    Yang LL, Kouri RE, Curren RD
    Carcinogenesis. 1984 ; 5 (2) : 291-294.
    PMID 6321052
    DNA structural elements required for ERCC1-XPF endonuclease activity.
    de Laat WL, Appeldoorn E, Jaspers NG, Hoeijmakers JH
    The Journal of biological chemistry. 1998 ; 273 (14) : 7835-7842.
    PMID 9525876
    Partial characterization of the DNA repair protein complex, containing the ERCC1, ERCC4, ERCC11 and XPF correcting activities.
    van Vuuren AJ, Appeldoorn E, Odijk H, Humbert S, Moncollin V, Eker AP, Jaspers NG, Egly JM, Hoeijmakers JH
    Mutation research. 1995 ; 337 (1) : 25-39.
    PMID 7596355


    This paper should be referenced as such :
    Stary, A ; Sarasin, A
    XPF (xeroderma pigmentosum, complementation group F)
    Atlas Genet Cytogenet Oncol Haematol. 2001;5(3):180-182.
    Free journal version : [ pdf ]   [ DOI ]
    On line version :

    Other Cancer prone implicated (Data extracted from papers in the Atlas) [ 2 ]
      Fanconi anemia Xeroderma pigmentosum

    External links

    HGNC (Hugo)ERCC4   3436
    LRG (Locus Reference Genomic)LRG_463
    Entrez_Gene (NCBI)ERCC4  2072  ERCC excision repair 4, endonuclease catalytic subunit
    AliasesERCC11; FANCQ; RAD1; XFEPS; 
    GeneCards (Weizmann)ERCC4
    Ensembl hg19 (Hinxton)ENSG00000175595 [Gene_View]
    Ensembl hg38 (Hinxton)ENSG00000175595 [Gene_View]  ENSG00000175595 [Sequence]  chr16:13920157-13952348 [Contig_View]  ERCC4 [Vega]
    ICGC DataPortalENSG00000175595
    TCGA cBioPortalERCC4
    AceView (NCBI)ERCC4
    Genatlas (Paris)ERCC4
    SOURCE (Princeton)ERCC4
    Genetics Home Reference (NIH)ERCC4
    Genomic and cartography
    GoldenPath hg38 (UCSC)ERCC4  -     chr16:13920157-13952348 +  16p13.12   [Description]    (hg38-Dec_2013)
    GoldenPath hg19 (UCSC)ERCC4  -     16p13.12   [Description]    (hg19-Feb_2009)
    GoldenPathERCC4 - 16p13.12 [CytoView hg19]  ERCC4 - 16p13.12 [CytoView hg38]
    Mapping of homologs : NCBIERCC4 [Mapview hg19]  ERCC4 [Mapview hg38]
    OMIM133520   278760   610965   615272   
    Gene and transcription
    Genbank (Entrez)AI470593 AK289726 AK301930 AK308341 BC020741
    RefSeq transcript (Entrez)NM_005236
    RefSeq genomic (Entrez)
    Consensus coding sequences : CCDS (NCBI)ERCC4
    Alternative Splicing GalleryENSG00000175595
    Gene ExpressionERCC4 [ NCBI-GEO ]   ERCC4 [ EBI - ARRAY_EXPRESS ]   ERCC4 [ SEEK ]   ERCC4 [ MEM ]
    Gene Expression Viewer (FireBrowse)ERCC4 [ Firebrowse - Broad ]
    SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
    BioGPS (Tissue expression)2072
    GTEX Portal (Tissue expression)ERCC4
    Human Protein AtlasENSG00000175595-ERCC4 [pathology]   [cell]   [tissue]
    Protein : pattern, domain, 3D structure
    Domain families : Pfam (Sanger)
    Domain families : Pfam (NCBI)
    Conserved Domain (NCBI)ERCC4
    DMDM Disease mutations2072
    Blocks (Seattle)ERCC4
    Human Protein Atlas [tissue]ENSG00000175595-ERCC4 [tissue]
    IPIIPI00219179   IPI00657932   
    Protein Interaction databases
    Ontologies - Pathways
    Huge Navigator ERCC4 [HugePedia]
    snp3D : Map Gene to Disease2072
    BioCentury BCIQERCC4
    Clinical trials, drugs, therapy
    Chemical/Protein Interactions : CTD2072
    Chemical/Pharm GKB GenePA27850
    Clinical trialERCC4
    canSAR (ICR)ERCC4 (select the gene name)
    DataMed IndexERCC4
    PubMed237 Pubmed reference(s) in Entrez
    GeneRIFsGene References Into Functions (Entrez)
    REVIEW articlesautomatic search in PubMed
    Last year publicationsautomatic search in PubMed

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    indexed on : Wed Feb 19 16:29:48 CET 2020

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