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ZNF217 (zinc finger protein 217)

Identity

Other names13009
FLJ14031
ZABC1
HGNC (Hugo) ZNF217
LocusID (NCBI) 7764
Location 20q13.2
Location_base_pair Starts at 52183610 and ends at 52199636 bp from pter ( according to hg19-Feb_2009)  [Mapping]

DNA/RNA

 
  Genomic organization of ZNF217. (A) The genomic organization of the five exons with encoded initiation and termination codons that make up ZNF217. Hatched boxes represent known 59- and 39-untranslated regions (UTR) in the cDNA. The sizes of exons and introns appear below and above the map, respectively. (B) The map of the 5632-bp ZNF217 cDNA. Vertical bars represent exon boundaries. The relative positions of the predicted eight C2H2 Kruppel-like zinc finger motifs are indicated by white circles. The position of the proline-rich putative transcription activator domain is shown as a hatched oval. AUUUA motifs are indicated in the 39-untranslated region. The relative locations of three ESTs are shown in boxes.
Description 5 exons
Transcription Exon 4 encodes a TGA termination codon and is alternatively processed.
Pseudogene None

Protein

 
  Eight C2H2 zinc fingers and a proline-rich domain. Conserved linker sequence, TGEKP, reported to bind DNA with high affinity
Description Full-length ZNF217 cDNAs encode two open reading frames of 1,062 and 1,108 amino acids, due to alternative splicing of exon 4.  Each predicted protein has eight C2H2 zinc fingers and a proline-rich domain.  Sequence analysis of ZNF217 indicates a strong resemblance to members of the Kruppel-like family of zinc finger proteins. The eight zinc finger domains in ZNF217 are interspersed throughout the ZNF217 sequence and their pattern does not appear to fall into one of the three classes of C2H2 zinc finger proteins; triple-C2H2, multiple­adjacent, and separated-paired fingers. The sixth and seventh zinc fingers in ZNF217 are separated by the conserved linker sequence, TGEKP, reported to bind DNA with high affinity.  Database analysis indicates that this paired zinc finger region aligns with those in several members of the Delta-EF1/ZFH-1 family of two-handed zinc-finger homeodomain proteins, including Smad-Interacting Protein 1 (SIP-1).
Expression ZNF217 is expressed at low levels in normal tissues.
Localisation Nuclear
Function ZNF217 protein localizes to the nucleus and co-immunoprecipitates with complexes containing the transcriptional corepressors CoREST and CtBP, histone deacetylases HDAC1 and HDAC2, and histone methyltransferases G9a and Eu-HMTase1.  This strongly suggests that ZNF217 may function as part of a transcriptional repressor complex.

Implicated in

Entity The findings that ZNF217 can immortalize human mammary epithelial cells, and that its amplification is associated with poor prognosis, suggest that it may play roles in both early and late stage breast cancer. ZNF217 can attenuate apoptotic signals resulting from telomere dysfunction as well as from doxorubicin-induced DNA damage, while silencing ZNF217 with siRNA restores sensitivity to doxorubicin. Moreover, elevated ZNF217 leads to increased phosphorylation of Akt, whereas inhibition of the phosphatidylinositol 3 kinase pathway and Akt phosphorylation decreases ZNF217 protein levels and increases sensitivity to doxorubicin. These results suggest that ZNF217 may promote neoplastic transformation by increasing cell survival during telomeric crisis, and may promote later stages of malignancy by increasing cell survival during chemotherapy.
  

External links

Nomenclature
HGNC (Hugo)ZNF217   13009
Cards
AtlasZNF217ID42875ch20q13
Entrez_Gene (NCBI)ZNF217  7764  zinc finger protein 217
GeneCards (Weizmann)ZNF217
Ensembl (Hinxton)ENSG00000171940 [Gene_View]  chr20:52183610-52199636 [Contig_View]  ZNF217 [Vega]
ICGC DataPortalENSG00000171940
cBioPortalZNF217
AceView (NCBI)ZNF217
Genatlas (Paris)ZNF217
WikiGenes7764
SOURCE (Princeton)NM_006526
Genomic and cartography
GoldenPath (UCSC)ZNF217  -  20q13.2   chr20:52183610-52199636 -  20q13.2   [Description]    (hg19-Feb_2009)
EnsemblZNF217 - 20q13.2 [CytoView]
Mapping of homologs : NCBIZNF217 [Mapview]
OMIM602967   
Gene and transcription
Genbank (Entrez)AF041259 AK290350 BC039055 BC113427
RefSeq transcript (Entrez)NM_006526
RefSeq genomic (Entrez)AC_000152 NC_000020 NC_018931 NT_011362 NW_001838666 NW_004929418
Consensus coding sequences : CCDS (NCBI)ZNF217
Cluster EST : UnigeneHs.711564 [ NCBI ]
CGAP (NCI)Hs.711564
Alternative Splicing : Fast-db (Paris)GSHG0019268
Alternative Splicing GalleryENSG00000171940
Gene ExpressionZNF217 [ NCBI-GEO ]     ZNF217 [ SEEK ]   ZNF217 [ MEM ]
Protein : pattern, domain, 3D structure
UniProt/SwissProtO75362 (Uniprot)
NextProtO75362  [Medical]
With graphics : InterProO75362
Splice isoforms : SwissVarO75362 (Swissvar)
Domaine pattern : Prosite (Expaxy)ZINC_FINGER_C2H2_1 (PS00028)    ZINC_FINGER_C2H2_2 (PS50157)   
Domains : Interpro (EBI)Znf_C2H2 [organisation]   Znf_C2H2-like [organisation]   Znf_C2H2/integrase_DNA-bd [organisation]  
Related proteins : CluSTrO75362
Domain families : Pfam (Sanger)zf-C2H2 (PF00096)   
Domain families : Pfam (NCBI)pfam00096   
Domain families : Smart (EMBL)ZnF_C2H2 (SM00355)  
DMDM Disease mutations7764
Blocks (Seattle)O75362
PDB (SRS)2HU2    3UK3    4F2J    4IS1   
PDB (PDBSum)2HU2    3UK3    4F2J    4IS1   
PDB (IMB)2HU2    3UK3    4F2J    4IS1   
PDB (RSDB)2HU2    3UK3    4F2J    4IS1   
Human Protein AtlasENSG00000171940 [gene] [tissue] [antibody] [cell] [cancer]
Peptide AtlasO75362
HPRD04272
IPIIPI00001150   IPI00979581   IPI00791663   
Protein Interaction databases
DIP (DOE-UCLA)O75362
IntAct (EBI)O75362
FunCoupENSG00000171940
BioGRIDZNF217
InParanoidO75362
Interologous Interaction database O75362
IntegromeDBZNF217
STRING (EMBL)ZNF217
Ontologies - Pathways
Ontology : AmiGOhistone deacetylase complex  sequence-specific DNA binding transcription factor activity  protein binding  nucleoplasm  transcription, DNA-templated  regulation of transcription, DNA-templated  transcription regulatory region DNA binding  negative regulation of transcription, DNA-templated  metal ion binding  
Ontology : EGO-EBIhistone deacetylase complex  sequence-specific DNA binding transcription factor activity  protein binding  nucleoplasm  transcription, DNA-templated  regulation of transcription, DNA-templated  transcription regulatory region DNA binding  negative regulation of transcription, DNA-templated  metal ion binding  
Protein Interaction DatabaseZNF217
Wikipedia pathwaysZNF217
Gene fusion - rearrangments
Polymorphisms : SNP, mutations, diseases
SNP Single Nucleotide Polymorphism (NCBI)ZNF217
snp3D : Map Gene to Disease7764
SNP (GeneSNP Utah)ZNF217
SNP : HGBaseZNF217
Genetic variants : HAPMAPZNF217
Exome VariantZNF217
1000_GenomesZNF217 
ICGC programENSG00000171940 
Somatic Mutations in Cancer : COSMICZNF217 
CONAN: Copy Number AnalysisZNF217 
Mutations and Diseases : HGMDZNF217
Mutations and Diseases : intOGenZNF217
Genomic VariantsZNF217  ZNF217 [DGVbeta]
dbVarZNF217
ClinVarZNF217
Pred. of missensesPolyPhen-2  SIFT(SG)  SIFT(JCVI)  Align-GVGD  MutAssessor  Mutanalyser  
Pred. splicesGeneSplicer  Human Splicing Finder  MaxEntScan  
Diseases
OMIM602967   
MedgenZNF217
GENETestsZNF217
Disease Genetic AssociationZNF217
Huge Navigator ZNF217 [HugePedia]  ZNF217 [HugeCancerGEM]
General knowledge
Homologs : HomoloGeneZNF217
Homology/Alignments : Family Browser (UCSC)ZNF217
Phylogenetic Trees/Animal Genes : TreeFamZNF217
Chemical/Protein Interactions : CTD7764
Chemical/Pharm GKB GenePA37588
Clinical trialZNF217
Cancer Resource (Charite)ENSG00000171940
Other databases
Probes
Litterature
PubMed40 Pubmed reference(s) in Entrez
CoreMineZNF217
iHOPZNF217
OncoSearchZNF217

Bibliography

Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma.
Collins C, Rommens JM, Kowbel D, Godfrey T, Tanner M, Hwang SI, Polikoff D, Nonet G, Cochran J, Myambo K, Jay KE, Froula J, Cloutier T, Kuo WL, Yaswen P, Dairkee S, Giovanola J, Hutchinson GB, Isola J, Kallioniemi OP, Palazzolo M, Martin C, Ericsson C, Pinkel D, Albertson D, Li WB, Gray JW
Proceedings of the National Academy of Sciences of the United States of America. 1998 ; 95 (15) : 8703-8708.
PMID 9671742
 
The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells.
Nonet GH, Stampfer MR, Chin K, Gray JW, Collins CC, Yaswen P
Cancer research. 2001 ; 61 (4) : 1250-1254.
PMID 11245413
 
In situ analyses of genome instability in breast cancer.
Chin K, de Solorzano CO, Knowles D, Jones A, Chou W, Rodriguez EG, Kuo WL, Ljung BM, Chew K, Myambo K, Miranda M, Krig S, Garbe J, Stampfer M, Yaswen P, Gray JW, Lockett SJ
Nature genetics. 2004 ; 36 (9) : 984-988.
PMID 15300252
 
The candidate oncogene ZNF217 is frequently amplified in colon cancer.
Rooney PH, Boonsong A, McFadyen MC, McLeod HL, Cassidy J, Curran S, Murray GI
The Journal of pathology. 2004 ; 204 (3) : 282-288.
PMID 15476264
 
Detection of Her2/neu, c-MYC and ZNF217 gene amplification during breast cancer progression using fluorescence in situ hybridization.
Shimada M, Imura J, Kozaki T, Fujimori T, Asakawa S, Shimizu N, Kawaguchi R
Oncology reports. 2005 ; 13 (4) : 633-641.
PMID 15756435
 
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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Contributor(s)

Written09-2005Paul Yaswen, Colin Collins
Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Bldg 977-225A, Berkeley, CA 94720-8174, USA

Citation

This paper should be referenced as such :
Yaswen, P ; Collins, C
ZNF217 (zinc finger protein 217)
Atlas Genet Cytogenet Oncol Haematol. 2006;10(1):7-8.
Free online version   Free pdf version   [Bibliographic record ]
URL : http://AtlasGeneticsOncology.org/Genes/ZNF217ID42875ch20q13.html

© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Sat Oct 4 12:44:56 CEST 2014

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