| Hybrid Gene |
| Note | The fusion occurs as a result of a cryptic, intrachromosomal rearrangement in tumors with apparently normal karyotypes. |
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| Map of the 8q11.2-q12 region including the PLAG1 and TCEA1 genes (not drawn to scale). Exons are shown as boxes and the start and stop codons are shown as asterisks and arrowheads, respectively. Reprinted partially from publication CHCHD7-PLAG1 and TCEA1-PLAG1 gene fusions resulting from cryptic, intrachromosomal 8q rearrangements in pleomorphic salivary gland adenomas, Genes Chromosomes Cancer, Vol. 45, No. 9, 2006, 820-828. Copyright 2006 Wiley-Liss, Inc. Reprinted with permission of Wiley-Liss, Inc. |
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| Description | The TCEA1-PLAG1 fusion transcript is formed by fusion of exon 1 of TCEA1 to exon 2 or 3 of PLAG1. |
| Detection | 1) RT-PCR using total RNA extracted from frozen tumor tissue. The TCEA1-PLAG1 fusion transcript was amplified by nested RT-PCR using the first round primers SII-UP, 5¹-CATGCGGTGGTGGGGGTTGCT-3', and MV5, 5'-CAGGAGAATGAGTAGCCATGTGC-3', and the second round primers S2-764S, 5'-GGGGTCGCTCCTGCTGTGTCT3' and MV6, 5'-TGCACTTGTAGGGCCTCTCTCCTG-3'. Fusion transcripts of 557 bp and 662 bp were detected. 2) Dual-color FISH and fiber-FISH on metaphase chromosomes using the BAC clones RP11-140I16 (PLAG1) and RP11-410P17 (TCEA1) as probes. |
| Fusion Protein |
| Expression Localisation | Nucleus. |
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| Salivary gland tumours. A review of 2410 cases with particular reference to histological types, site, age and sex distribution. |
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| CHCHD7-PLAG1 and TCEA1-PLAG1 gene fusions resulting from cryptic, intrachromosomal 8q rearrangements in pleomorphic salivary gland adenomas. |
| Asp J, Persson F, Kost-Alimova M, Stenman G. |
| Genes Chromosomes Cancer. 2006; 45:820-828. |
| PMID 16736500 |
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