t(3;5)(q25;q35) as a sole anomaly in acute myeloid leukemia patient
2007-05-01 Adriana Zamecnikova AffiliationKuwait Cancer Control Center, Lab of Cancer Genetics, Department of Hem, Shuwaikh, POBox 42262, Shuwaikh, 70653 Kuwait
Previous history
Preleukaemia
-
Malignant disease
-
Inborn condition
-
Clinics case report
Age
36 yrs
Sex
F
Liver
-
Spleen
-
Lymph nodes
-
Cns involv
-
Blood data
Wbc
1.9
Hb
10.0
Platelets
41
Blasts
11
Bone marrow
Peroxidase positive.
Cyto path
Cytology
Acute Myeloid Leukemia.
Immunophenotype
Not available.
Rearranged ig tcr
-
Pathology
-
Electron microscopy
-
Precise diagnosis
Acute Myeloid Leukemia, M2.
Survival data
Date diagnosis
(03-2007)
Treatment
Left for treatment abroad.
Complete remission
+/-. Not known as the patient left for treatment abroad.
Treatment relat death
-
Relapse
-
Phenotype relapse
-
Status
L
Date last follow
(03-2007)
Survival
1 +
Karyotype
Sample
BM
Culture time
24
Banding
G-band
Results
46,XY,t(3;5)(q25;q35)
Mol cytogenet technics
Fluorescence in situ hybridisation (FISH), with LSI 5q EGR1 SO/D5S23 SG) and LSI BCL6 DC probes obtained from Vysis (Downers Grove IL, USA).
Mol cytogenet results
Analysis with LSI 5q EGR1 SO/D5S23 SG probe revealed one red and green signal on normal chromosome 5 and on der(5) chromosome. Analysis with LSI BCL6 DC probe revealed one fusion signal on normal chromosome 3 and a fusion signal on der(5) distal to EGR1 locus confirming the t(3;5).
Images
Karyotype of the patient demonstrating the t(3;5)(q25;q35).
LSI BCL6 DC, Break Apart Rearrangement Probe exhibiting one red/green fusion on normal chromosome 3 and fusion signal on der(5) distal to EGR1. Hybridization with LSI 5q EGR1 SO/D5S23 SG probe on metaphase showing one red and green signal on normal and der(5) chromosome.
Comments section
Comments
The chromosomal translocation t(3;5)(q25;q35) was observed only in individual cases. From the 5 described cases, 3 cases (2 male 1 female) were diagnosed with MDS and 2 cases with AML-M6 (1 male 1 female) suggesting the rearrangement with possible involvement of NPM/MLF1 genes is associated with myeloid malignancies.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 1450412 | 1992 | Clinical, morphologic, and cytogenetic characteristics of 26 patients with acute erythroblastic leukemia. | Olopade OI et al |
| 14506644 | 2003 | Detection of NPM/MLF1 fusion in t(3;5)-positive acute myeloid leukemia and myelodysplasia. | Arber DA et al |
| 16341035 | 2006 | Loss of the NPM1 gene in myeloid disorders with chromosome 5 rearrangements. | Berger R et al |
Citation
Adriana Zamecnikova
t(3;5)(q25;q35) as a sole anomaly in acute myeloid leukemia patient
Atlas Genet Cytogenet Oncol Haematol. 2007-05-01
Online version: http://atlasgeneticsoncology.org/case-report/208823/t(3
