NONO (non-POU domain containing, octamer-binding)

1999-01-01   Jean-Loup Huret 

Genetics, Dept Medical Information, University of Poitiers, CHU Poitiers Hospital, F-86021 Poitiers, France

Identity

HGNC
LOCATION
Xq13.1
LOCUSID
FUSION GENES

DNA/RNA

Transcription

alternative splicing; 2.4 mRNA complete cds; coding sequence: CDS 129 ... 1550

Proteins

Atlas Image

Description

the protein encoded by NONO is called p54nrb (nuclear RNA binbing); 471 amino acids; 54 kDa; N-term glutamine/histidine rich region, tandem RNA binding domains (amino acids 75 to 228), helix-turn-helix implicated in DNA binding, and a proline rich region in C-term; N-term and C-term allow protein-protein interactions.

Expression

wide

Function

binds independantly to DNA and RNA; forms a heterodimer with PSF, a protein sharing vast homologies; both form complexes with DNA topoisomerase I, which renders this enzyme much more active; NONO also enhances the binding of several DNA-binding proteins (but not all).

Homology

with PSF and other proteins with a DBHS domain (Drosophila behaviour, human splicing) which includes the tandem RNA binding domains.

Implicated in

Entity name
inv(X)(p11.2q12) in renal cell carcinoma -->NONO/TFE3
Disease
only one case of papillary renal cell carcinoma
Hybrid gene
5 NONO- 3 TFE3
Atlas Image
Fusion protein
N-term NONO and most of it except the C-term proline rich sequence fused to the DNA binding domains of TFE3 (excluding the acidic transcriptional activation domain, including the C-term helix-loop-helix, and the leucine zipper); the reciprocal transcript is found

Bibliography

Pubmed IDLast YearTitleAuthors
93939821997Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma.Clark J et al
83719831993Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6.Dong B et al
97568481998The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction.Straub T et al
83557021993NonO, a non-POU-domain-containing, octamer-binding protein, is the mammalian homolog of Drosophila nonAdiss.Yang YS et al
91710771997NonO enhances the association of many DNA-binding proteins to their targets.Yang YS et al

Other Information

Locus ID:

NCBI: 4841
MIM: 300084
HGNC: 7871
Ensembl: ENSG00000147140

Variants:

dbSNP: 4841
ClinVar: 4841
TCGA: ENSG00000147140
COSMIC: NONO

RNA/Proteins

Gene IDTranscript IDUniprot
ENSG00000147140ENST00000276079Q15233
ENSG00000147140ENST00000276079A0A0S2Z4Z9
ENSG00000147140ENST00000373841Q15233
ENSG00000147140ENST00000373841A0A0S2Z4Z9
ENSG00000147140ENST00000373856Q15233
ENSG00000147140ENST00000373856A0A0S2Z4Z9
ENSG00000147140ENST00000413858C9JYS8
ENSG00000147140ENST00000418921H7C367
ENSG00000147140ENST00000420903C9J4X2
ENSG00000147140ENST00000450092C9JJ13
ENSG00000147140ENST00000454976C9IZL7
ENSG00000147140ENST00000535149Q15233

Expression (GTEx)

0
50
100
150
200
250

Protein levels (Protein atlas)

Not detected
Low
Medium
High

References

Pubmed IDYearTitleCitations
124172962002PSF and p54(nrb)/NonO--multi-functional nuclear proteins.144
176390832007The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination.102
161480432005P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner.94
150572752004p54(nrb) associates with the 5' splice site within large transcription/splicing complexes.82
204217352010Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response.78
129444872003PSF acts through the human immunodeficiency virus type 1 mRNA instability elements to regulate virus expression.68
191655272009Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.56
124034702002PSF and p54nrb bind a conserved stem in U5 snRNA.54
224161262012Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation.54
155906772005Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor.52

Citation

Jean-Loup Huret

NONO (non-POU domain containing, octamer-binding)

Atlas Genet Cytogenet Oncol Haematol. 1999-01-01

Online version: http://atlasgeneticsoncology.org/gene/168/nono-(non-pou-domain-containing-octamer-binding)