EEF1A2 (eukaryotic translation elongation factor 1 alpha 2)

2008-04-01   Sanna Noubir , Jonathan M Lee 

Department of Biochemistry, Microbiology, Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5.

Identity

HGNC
LOCATION
20q13.33
LOCUSID
ALIAS
DEE33,EEF1AL,EF-1-alpha-2,EF1A,EIEE33,HS1,MRD38,STN,STNL
FUSION GENES

DNA/RNA

Description

8 exons and 7 introns.

Transcription

5 alternatives for transcript splicing.

Proteins

Atlas Image

Description

50kDa; 463 amino acids contains tRNA binding sites, GTP binding and hydrolysis sites and putative actin binding sites (see figure above).

Expression

In mice and humans, eEF1A2 is expressed only in normal tissues of the brain, heart and skeletal muscle. Expression is found in tumours of the breast, lung and ovary.

Localisation

Diffusely cytoplasmic and nuclear.

Function

This protein is an isoform of the alpha subunit of the elongation factor-1 complex. There are two known isoforms of protein elongation factor eEF1A (eEF1A1 and eEF1A2). eEF1A proteins are GTP-binding proteins that interact with amino-acylated tRNA and recruit them to the ribosome during the elongation phase of protein translation. In addition, eEF1A2 binds to and stimulates the lipid kinase activity of phosphatidylinositol 4-kinase beta, the enzyme that catalyzes the generation of phosphatidylinositol 4-phosphate from phosphatidylinositol. eEF1A2 also activates the Akt serine/threonine kinase and can stimulate actin remodeling and increase the rate of cell invasion and migration in vitro.

Homology

EEF1A2 is highly homologous to the EEF1A1. EEF1A2 and EEF1A1 share greater than 90% DNA sequence and amino acid identity. The GTP binding and hydrolysis domains also have homology to the Ras GTPase.

Mutations

Note

Inactivation of mouse EEF1A2 leads to immunodeficiency, neuromuscular abnormalities and death by 30 days of age.

Implicated in

Entity name
Tumorigenesis
Disease
Breast, lung and ovarian cancer. EEF1A2 overexpression is found in approximatively 60% of breast, 30% of ovary and 40% of lung tumours.
Prognosis
High eEF1A2 protein expression is associated with good prognosis in both breast and ovarian cancer. High eEF1A2 protein expression predicts poor prognosis in lung cancer.
Cytogenetics
Fluorescence in situ hybridization (FISH), showed high copy numbers of EEF1A2 in 25% ovarian tumors.
Oncogenesis
The eEF1A2 gene is transforming and increases the in-vitro growth rate of mouse and human cells grown in-vitro and also enhances their tumorigenicity in mouse xenograft models.

Bibliography

Pubmed IDLast YearTitleAuthors
171308422007eEF1A2 activates Akt and stimulates Akt-dependent actin remodeling, invasion and migration.Amiri A et al
120531772002Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer.Anand N et al
170882552007Binding of elongation factor eEF1A2 to phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and phosphatidylinositol 4-phosphate generation.Jeganathan S et al
168974282007Expression of protein elongation factor eEF1A2 predicts favorable outcome in breast cancer.Kulkarni G et al
181647512008The prognostic significance of elongation factor eEF1A2 in ovarian cancer.Pinke DE et al
174370102007Expression of eEF1A2 is associated with clear cell histology in ovarian carcinomas: overexpression of the gene is not dependent on modifications at the EEF1A2 locus.Tomlinson VA et al

Other Information

Locus ID:

NCBI: 1917
MIM: 602959
HGNC: 3192
Ensembl: ENSG00000101210

Variants:

dbSNP: 1917
ClinVar: 1917
TCGA: ENSG00000101210
COSMIC: EEF1A2

RNA/Proteins

Gene IDTranscript IDUniprot
ENSG00000101210ENST00000217182Q05639
ENSG00000101210ENST00000298049A0A2U3TZH3
ENSG00000101210ENST00000642899A0A2R8Y488
ENSG00000101210ENST00000645357A0A2R8Y660
ENSG00000101210ENST00000646335A0A2R8YDN5

Expression (GTEx)

0
500
1000
1500
2000
2500

Pathways

PathwaySourceExternal ID
RNA transportKEGGko03013
RNA transportKEGGhsa03013
LegionellosisKEGGko05134
LegionellosisKEGGhsa05134
Metabolism of proteinsREACTOMER-HSA-392499
TranslationREACTOMER-HSA-72766
Eukaryotic Translation ElongationREACTOMER-HSA-156842
Gene ExpressionREACTOMER-HSA-74160

Protein levels (Protein atlas)

Not detected
Low
Medium
High

References

Pubmed IDYearTitleCitations
120531772002Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer.62
171308422007eEF1A2 activates Akt and stimulates Akt-dependent actin remodeling, invasion and migration.42
161568882005Translation elongation factor eEF1A2 is a potential oncoprotein that is overexpressed in two-thirds of breast tumours.41
163694912006Identification of putative oncogenes in lung adenocarcinoma by a comprehensive functional genomic approach.40
189782492008Effects of cardiovascular medications on rate of functional decline in Alzheimer disease.37
246972192015De novo EEF1A2 mutations in patients with characteristic facial features, intellectual disability, autistic behaviors and epilepsy.30
236085342013Degradation of newly synthesized polypeptides by ribosome-associated RACK1/c-Jun N-terminal kinase/eukaryotic elongation factor 1A2 complex.28
183228132008Statin use and prostate cancer risk in a large population-based setting.27
208645122010A panel of sputum-based genomic marker for early detection of lung cancer.26
205057612010Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomas.21

Citation

Sanna Noubir ; Jonathan M Lee

EEF1A2 (eukaryotic translation elongation factor 1 alpha 2)

Atlas Genet Cytogenet Oncol Haematol. 2008-04-01

Online version: http://atlasgeneticsoncology.org/gene/40408/eef1a2-(eukaryotic-translation-elongation-factor-1-alpha-2)