NET1 (neuroepithelial cell transforming gene 1)
2008-04-01 David Murray , Peter Doran AffiliationUCD School of Medicine, Medical Sciences, UCD-Mater Clinical Research Centre, Mater Misericordiae University Hospital, Dublin 7, Ireland
Identity
HGNC
LOCATION
10p15.1
LOCUSID
ALIAS
ARHGEF8,NET1A
FUSION GENES
DNA/RNA
Description
This gene, found on chromosome 10 at location 10p15 (5,444,518-5,490,401) encompasses 11832bp of genomic DNA. The gene has 10 exons and the mRNA transcript is 3414 nucleotides in length.
Proteins
Expression
This protein is widely expressed in mammalian tissue.
Localisation
It is localized to both the cytoplasm and the nucleus.
Function
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase.
Homology
Holomogy with Pan troglodytes (97%); Canis lupus familiaris (92%); Mus musculus (93%), Rattus norvegicus (92%), Gallus gallus (88%).
Description
The protein contains the following domains : a Rho guanine nucleotide exchange factor (RhoGEF) activity domain from amino acid 178 - 355 and a pleckstrin homology domain from amino acid 387 - 503.
The 596 amino acid sequence is :
1 mepelaaqkq prprrrsrra sglstegatg psadtsgsel dgrcslrrgs sftfltpgpn
61 wdftlkrkrr ekdddvvsls sldlkepsnk rvrplarvts lanlispvrn gavrrfgqti
121 qsftlrgdhr spasaqkfss rstvptpakr rssalwseml ditmkesltt reirrqeaiy
181 emsrgeqdli edlklarkay hdpmlklsim seeelthifg dldsyiplhe dlltrigeat
241 kpdgtveqig hilvswlprl nayrgycsnq laakalldqk kqdprvqdfl qrclespfsr
301 kldlwsfldi prsrlvkypl llkeilkhtp kehpdvqlle dailiiqgvl sdinlkkges
361 ecqyyidkle yldekqrdpr ieaskvllch gelrsksghk lyiflfqdil vltrpvtrne
421 rhsyqvyrqp ipvqelvled lqdgdvrmgg sfrgafsnse kaknifrirf hdpspaqsht
481 lqandvfhkq qwfnciraai apfqsagspp elqglpelhe ecegnhpsar kltaqrrast
541 vssvtqvevd enayrcgsgm qmaedskslk thqtqpgirr ardkalsggk rketlv
The 596 amino acid sequence is :
1 mepelaaqkq prprrrsrra sglstegatg psadtsgsel dgrcslrrgs sftfltpgpn
61 wdftlkrkrr ekdddvvsls sldlkepsnk rvrplarvts lanlispvrn gavrrfgqti
121 qsftlrgdhr spasaqkfss rstvptpakr rssalwseml ditmkesltt reirrqeaiy
181 emsrgeqdli edlklarkay hdpmlklsim seeelthifg dldsyiplhe dlltrigeat
241 kpdgtveqig hilvswlprl nayrgycsnq laakalldqk kqdprvqdfl qrclespfsr
301 kldlwsfldi prsrlvkypl llkeilkhtp kehpdvqlle dailiiqgvl sdinlkkges
361 ecqyyidkle yldekqrdpr ieaskvllch gelrsksghk lyiflfqdil vltrpvtrne
421 rhsyqvyrqp ipvqelvled lqdgdvrmgg sfrgafsnse kaknifrirf hdpspaqsht
481 lqandvfhkq qwfnciraai apfqsagspp elqglpelhe ecegnhpsar kltaqrrast
541 vssvtqvevd enayrcgsgm qmaedskslk thqtqpgirr ardkalsggk rketlv
Mutations
Note
NET1 is subject to the following misense mutations :
| Source ID: | rs34821922 | rs34658946 | rs34821949 |
| Wild type nucleotide: | A | T | A |
| Wild type amino acid: | K | I | K |
| Variant nucleotide: | G | C | G |
| Variant amino acid: | R | T | E |
| Amino Acid Position: | 28 | 363 | 466 |
Implicated in
Entity name
Gastric Cancer
Disease
Gastric or stomach cancer is a malignant epithelial malignancy of the stomach mucosa. The vast majority of gastric carcinomas are adenocarcinomas, arising from the gastric glandular epithelium.
Prognosis
Prognosis depends on the stage of the cancer. Because most stomach cancers are at an advanced stage upon diagnosis, the average 5 years survival rate is 12%. This is often due to the fact that the tumor has spread beyond the primary site.
Oncogenesis
NET1 has been shown to be up-regulated in Gastric Cancer (Leyden J et al., 2006). NET1 is a GEF exchange factor for Rho GTPase proteins (Alberts AS et al., 1998) and is therefore implicated in Rho-mediated processes such as cytoskeletal organization and cell migration. NET1 has been shown to drive gastric tumor cell invasion (Leyden J et al., 2006). It is this aggressive phenotype of the disease that accounts for the dismal prognosis as the majority of gastric tumors are at an advanced stage upon diagnosis where they have spread beyond the primary site.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 15684429 | 2005 | PAK1 negatively regulates the activity of the Rho exchange factor NET1. | Alberts AS et al |
| 8649828 | 1996 | Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. | Chan AM et al |
| 11350080 | 2001 | Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1. | Dobrosotskaya IY et al |
| 17938206 | 2007 | The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. | García-Mata R et al |
| 16344560 | 2006 | Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. | Kimura K et al |
| 16552434 | 2006 | Net1 and Myeov: computationally identified mediators of gastric cancer. | Leyden J et al |
| 15611121 | 2005 | Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1. | Qin H et al |
| 11278519 | 2001 | The activity of guanine exchange factor NET1 is essential for transforming growth factor-beta-mediated stress fiber formation. | Shen X et al |
Other Information
Locus ID:
NCBI: 10276
MIM: 606450
HGNC: 14592
Ensembl: ENSG00000173848
Variants:
dbSNP: 10276
ClinVar: 10276
TCGA: ENSG00000173848
COSMIC: NET1
RNA/Proteins
Expression (GTEx)
Pathways
Protein levels (Protein atlas)
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 37171948 | 2023 | Association between NET-1 Level in Placenta from Preeclampsia Pregnancies and Trophoblast Proliferation and Apoptosis: A Correlation Analysis. | 0 |
| 37171948 | 2023 | Association between NET-1 Level in Placenta from Preeclampsia Pregnancies and Trophoblast Proliferation and Apoptosis: A Correlation Analysis. | 0 |
| 34957935 | 2022 | Circ_0000745 promotes acute lymphoblastic leukemia progression through mediating miR-494-3p/NET1 axis. | 17 |
| 34957935 | 2022 | Circ_0000745 promotes acute lymphoblastic leukemia progression through mediating miR-494-3p/NET1 axis. | 17 |
| 31910717 | 2021 | Monoaminergic Genetic Variants, Prefrontal Cortex-Amygdala Circuit, and Emotional Symptoms in Children With ADHD: Exploration Based on the Gene-Brain-Behavior Relationship. | 2 |
| 33465404 | 2021 | Cdk1 phosphorylation negatively regulates the activity of Net1 towards RhoA during mitosis. | 3 |
| 33740419 | 2021 | Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling. | 16 |
| 33839702 | 2021 | NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway. | 3 |
| 31910717 | 2021 | Monoaminergic Genetic Variants, Prefrontal Cortex-Amygdala Circuit, and Emotional Symptoms in Children With ADHD: Exploration Based on the Gene-Brain-Behavior Relationship. | 2 |
| 33465404 | 2021 | Cdk1 phosphorylation negatively regulates the activity of Net1 towards RhoA during mitosis. | 3 |
| 33740419 | 2021 | Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling. | 16 |
| 33839702 | 2021 | NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway. | 3 |
| 30332929 | 2020 | Regulation of RhoA activation and cell motility by c-Jun N-terminal kinases and Net1. | 5 |
| 31451992 | 2020 | LncRNA CTC-497E21.4 promotes the progression of gastric cancer via modulating miR-22/NET1 axis through RhoA signaling pathway. | 0 |
| 32420320 | 2020 | Neuroepithelial Cell Transforming Gene 1 Acts as an Oncogene and Is Mediated by miR-22 in Human Non-Small-Cell Lung Cancer. | 1 |
Citation
David Murray ; Peter Doran
NET1 (neuroepithelial cell transforming gene 1)
Atlas Genet Cytogenet Oncol Haematol. 2008-04-01
Online version: http://atlasgeneticsoncology.org/gene/41526/net1-(neuroepithelial-cell-transforming-gene-1)
