CENPW (centromere protein W)
2013-06-01 Seyoung Jeon , Soojin Lee AffiliationDepartment of Microbiology, Molecular Biology, Chungnam National Institute, 305-764, Daejeon, Republic of Korea
DNA/RNA
Figure 1. A genomic structure of the CENPW gene.
Description
Exons: 3, coding exons: 3, introns: 2.
Transcription
Transcript length: 750 bps, open reading frame: 264 bps.
Pseudogene
No pseudogene.
Proteins
Figure 2. Schematic representation of CENPW protein. Each domain is represented with the unique color and name. One nuclear localization signal (NLS) sequence of 17 amino acids starts at amino acid 13.
Description
CENP-W gene encodes 88-amino acid protein which shows ~27% of amino acid sequence similarity to a general transcription repressor, human DR1. It contains a typical NLS sequence and histone fold region which is critical for binding to centromeric DNA.
Expression
Widely expressed; kidney, colon, breast, liver, lung, ovary, prostate, thyroid, etc.
Localisation
Intracellular, nucleus, centromere, kinetochore, nucleus matrix, nucleolus.
Function
CENP-W was originally identified as a cancer-upregulated gene 2 (CUG2) which is commonly overexpressed in various human cancer tissues. Although it has high oncogenic activities, CENP-W also induces cell apoptosis when overexpressed in certain cell lines. After it was revealed that CENP-W forms a stable heterodimer with CENP-T and is localized in kinetochores during mitosis, CENP-W become recognized as a new member of the inner centromere protein complex. Subsequent studies have also shown that CENP-T-W-S-X forms a unique centromeric nucleosome-like heterotetramer structure which binds to and supercoils DNA. As a nucleolus-associated protein, CENP-W may play critical role in the formation of functional kinetochore, possibly by facilitating the recruitment of other centromeric components during interphase, which is required for proper chromosome segregation during mitosis.
Homology
According to NCBI-HomoloGene:
Pan troglodytes (chimpanzee): centromere protein W (NP_001012525.1, 88 aa)
Macacamulatta (Rhesus monkey): centromere protein W (XP_001107034.2, 88 aa)
Canis lupus familiaris (dog): centromere protein W-like (XP_003638832.1, 88 aa)
Bostaurus (cattle): centromere protein W (NP_001104731.1, 88 aa)
Mus musculus (house mouse): centromere protein W (NP_001103217.1, 86 aa)
Rattus norvegicus (Norway rat): centromere protein W (XP_001070657.1, 86 aa).
Pan troglodytes (chimpanzee): centromere protein W (NP_001012525.1, 88 aa)
Macacamulatta (Rhesus monkey): centromere protein W (XP_001107034.2, 88 aa)
Canis lupus familiaris (dog): centromere protein W-like (XP_003638832.1, 88 aa)
Bostaurus (cattle): centromere protein W (NP_001104731.1, 88 aa)
Mus musculus (house mouse): centromere protein W (NP_001103217.1, 86 aa)
Rattus norvegicus (Norway rat): centromere protein W (XP_001070657.1, 86 aa).
Mutations
Note
Over expressed in various cancer tissues; notably high in the ovary (6.3-fold), liver (6.0), lung (4.9), and pancreas (3.8).
Figure 3. mRNA expression of CUG2 in various tumors. DNA chip microarray profile in tumors (closed bar) and normal (open bar) tissues from the Gene Express Oncology DatasuiteTM of Gene Logic Inc.
Implicated in
Entity name
Various human cancers
Oncogenesis
CENP-W has been found to be upregulated in several tumor tissues. When the expression level of CENP-W was examined by DNA chip microarray and RT-PCR in tumors, six tissues (ovary, liver, lung, pancreas, colon, and stomach) showed high-fold increases in expression profiles. Also, CENP-W-transformed NIH3T3 cells exhibited exceptionally prominent cancerous phenotypes in vitro as well as in vivo tumor forming assays.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 22002061 | 2011 | New centromeric component CENP-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein. | Chun Y et al |
| 19533040 | 2009 | Cancer-upregulated gene 2 (CUG2), a new component of centromere complex, is required for kinetochore function. | Kim H et al |
| 20180024 | 2010 | Sp1 and Sp3 mediate basal and serum-induced expression of human CENP-W. | Kim H et al |
| 21838932 | 2011 | Cug2 is essential for normal mitotic control and CNS development in zebrafish. | Kim HT et al |
| 17610844 | 2007 | Molecular cloning and functional analysis of a novel oncogene, cancer-upregulated gene 2 (CUG2). | Lee S et al |
| 20648695 | 2010 | Cancer-upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV-3 cells. | Lee S et al |
| 22304917 | 2012 | CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold. | Nishino T et al |
| 20075984 | 2010 | CUG2, a novel oncogene confers reoviral replication through Ras and p38 signaling pathway. | Park EH et al |
| 21695110 | 2011 | Premitotic assembly of human CENPs -T and -W switches centromeric chromatin to a mitotic state. | Prendergast L et al |
Other Information
Locus ID:
NCBI: 387103
MIM: 611264
HGNC: 21488
Ensembl: ENSG00000203760
Variants:
dbSNP: 387103
ClinVar: 387103
TCGA: ENSG00000203760
COSMIC: CENPW
RNA/Proteins
| Gene ID | Transcript ID | Uniprot |
|---|---|---|
| ENSG00000203760 | ENST00000368325 | Q5EE01 |
| ENSG00000203760 | ENST00000368326 | A0A0A0MRK5 |
| ENSG00000203760 | ENST00000368328 | Q5EE01 |
Expression (GTEx)
Pathways
References
| Pubmed ID | Year | Title | Citations |
|---|---|---|---|
| 35000669 | 2022 | Translocalization of enhanced PKM2 protein into the nucleus induced by cancer upregulated gene 2 confers cancer stem cell-like phenotypes. | 0 |
| 35000669 | 2022 | Translocalization of enhanced PKM2 protein into the nucleus induced by cancer upregulated gene 2 confers cancer stem cell-like phenotypes. | 0 |
| 33973496 | 2021 | Knockdown of CENPW Inhibits Hepatocellular Carcinoma Progression by Inactivating E2F Signaling. | 6 |
| 34416157 | 2021 | Brain-trait-associated variants impact cell-type-specific gene regulation during neurogenesis. | 24 |
| 33973496 | 2021 | Knockdown of CENPW Inhibits Hepatocellular Carcinoma Progression by Inactivating E2F Signaling. | 6 |
| 34416157 | 2021 | Brain-trait-associated variants impact cell-type-specific gene regulation during neurogenesis. | 24 |
| 30267325 | 2019 | Two major alternative splice variants of beta-TrCP1 interact with CENP-W with different binding preferences. | 1 |
| 30771899 | 2019 | Cancer upregulated gene (CUG)2 elevates YAP1 expression, leading to enhancement of epithelial-mesenchymal transition in human lung cancer cells. | 15 |
| 30968157 | 2019 | CGK062, a small chemical molecule, inhibits cancer upregulated gene 2‑induced oncogenesis through NEK2 and β‑catenin. | 7 |
| 31113615 | 2019 | Cancer upregulated gene 2 (CUG2), a novel oncogene, promotes stemness-like properties via the NPM1-TGF-β signaling axis. | 11 |
| 30267325 | 2019 | Two major alternative splice variants of beta-TrCP1 interact with CENP-W with different binding preferences. | 1 |
| 30771899 | 2019 | Cancer upregulated gene (CUG)2 elevates YAP1 expression, leading to enhancement of epithelial-mesenchymal transition in human lung cancer cells. | 15 |
| 30968157 | 2019 | CGK062, a small chemical molecule, inhibits cancer upregulated gene 2‑induced oncogenesis through NEK2 and β‑catenin. | 7 |
| 31113615 | 2019 | Cancer upregulated gene 2 (CUG2), a novel oncogene, promotes stemness-like properties via the NPM1-TGF-β signaling axis. | 11 |
| 30226605 | 2018 | STAT1‑HDAC4 signaling induces epithelial‑mesenchymal transition and sphere formation of cancer cells overexpressing the oncogene, CUG2. | 23 |
Citation
Seyoung Jeon ; Soojin Lee
CENPW (centromere protein W)
Atlas Genet Cytogenet Oncol Haematol. 2013-06-01
Online version: http://atlasgeneticsoncology.org/gene/51452/cenpw-(centromere-protein-w)
