t(9;9)(p13;p24) PAX5/JAK2
del(9)(p13p24) PAX5/JAK2
inv(9)(p13p24) PAX5/JAK2

2014-03-01   Jean-Loup Huret  

1.Genetics, Dept Medical Information, University of Poitiers, CHU Poitiers Hospital, F-86021 Poitiers, France

Clinics and Pathology

Disease

B-cell acute lymphoblastic leukemia (B-ALL)

Phenotype stem cell origin

Three cases were CD10+, and one case was Cμ.

Embryonic origin

Only four cases to date (Nebral et al., 2009; Coyaud et al., 2010; Roberts et al., 2012).

Epidemiology

This chromosome abnormality has only been found so far in childhood B-ALL: there were 2 male and 2 female patients, aged 7, 10, 13 and 14 years.

Prognosis

Two of three patients were considered as being at high risk, and one at intermediate risk. One patient was in complete remission (CR) 65 months after diagnosis, and another one was in a second CR at 10 months+ (Nebral et al., 2009).

Genes Involved and Proteins

Gene name
JAK2 (janus kinase 2)
Location
9p24.1
Protein description
1132 amino acids (aa); from N-term to C-term, JAK2 contains: an interaction region with cytokine/interferon/growth hormone receptors: aa 1-239, a FERM domain: aa 37-380, a SH2 domain: aa 401-482, two protein kinase domains: aa 545-809 and 849-1124, an ATP nucleotide binding site: aa 855-863, and a loop structure: aa 1056-1078 (JAK2 kinase insertion loop). JAK homology domains are the following: JH7: aa 25-137; JH6: aa 144-284; JH5: aa 288-309; JH4: aa 322-440; JH3: aa 451-538; JH2: aa 543-824; JH1: 836-1123. Phosphotyrosines are located at aa 119, 372, 373, 523, 813, 868, 966, 972, 1007, and 1008 (Harpur et al., 1992; Saltzman et al., 1998; Lucet et al., 2006). Protein tyrosine kinase of the non-receptor type that associates with the intracellular domains of cytokine receptors; Mediates signaling transduction.
Gene name
PAX5 (paired box gene 5)
Location
9p13.2
Protein description
391 amino acids; from N-term to C-term, PAX5 contains: a paired domain (aa: 16-142); an octapeptide (aa: 179-186); a partial homeodomain (aa: 228-254); a transactivation domain (aa: 304-359); and an inhibitory domain (aa: 359-391). Lineage-specific transcription factor; recognizes the concensus recognition sequence GNCCANTGAAGCGTGAC, where N is any nucleotide. Involved in B-cell differentiation. Entry of common lymphoid progenitors into the B cell lineage depends on E2A, EBF1, and PAX5; activates B-cell specific genes and repress genes involved in other lineage commitments. Activates the surface cell receptor CD19 and repress FLT3. Pax5 physically interacts with the RAG1/RAG2 complex, and removes the inhibitory signal of the lysine-9-methylated histone H3, and induces V-to-DJ rearrangements. Genes repressed by PAX5 expression in early B cells are restored in their function in mature B cells and plasma cells, and PAX5 repressed (Fuxa et al., 2004; Johnson et al., 2004; Zhang et al., 2006; Cobaleda et al., 2007; Medvedovic et al., 2011).

Result of the Chromosomal Anomaly

Description

Fusion of PAX5 exon 5 to JAK2 exon 19 in each case. Opposite direction is noted by Coyaud et al., 2010.
Atlas Image
PAX5/JAK2 fusion protein.

Description

522 amino acids (201 from PAX5 and 321 from JAK2). The predicted fusion protein contains the DNA binding paired domain of PAX5 and the Protein kinase 2 domain from JAK2 (breakpoint at aa 811 or 812 in JAK2).

Highly cited references

Pubmed IDYearTitleCitations
255159602015The role of the Janus-faced transcription factor PAX5-JAK2 in acute lymphoblastic leukemia.25
295070762018Prognostic impact of kinase-activating fusions and IKZF1 deletions in pediatric high-risk B-lineage acute lymphoblastic leukemia.12
278701512017Ph-like acute lymphoblastic leukemia with a novel PAX5-KIDINS220 fusion transcript.5
351567272022The PAX5-JAK2 translocation acts as dual-hit mutation that promotes aggressive B-cell leukemia via nuclear STAT5 activation.3

Article Bibliography

Pubmed IDLast YearTitleAuthors

Summary

Fusion gene

PAX5/JAK2 PAX5 (9p13.2) JAK2 (9p24.1) COF 1071 1072|PAX5/JAK2 PAX5 (9p13.2) JAK2 (9p24.1) M del(9)(p13) del(9)(p13p24) del(9)(p24) inv(9)(p13p24) t(9;9)(p13;p24)|PAX5/JAK2 PAX5 (9p13.2) JAK2 (9p24.1) TIC

Citation

Jean-Loup Huret

t(9;9)(p13;p24) PAX5/JAK2
del(9)(p13p24) PAX5/JAK2
inv(9)(p13p24) PAX5/JAK2

Atlas Genet Cytogenet Oncol Haematol. 2014-03-01

Online version: http://atlasgeneticsoncology.org/haematological/1559/js/lib/img/welcome